Abstract：
:The major site of B-cell genesis in the sheep is the ileal Peyer's patch (PP). The B cells in the ileal PP undergo both extensive proliferation and massive death in association with an ongoing diversification of the immunoglobulin repertoire by somatic hypermutation. Most, if not all, the B-cell death in the ileal PP is due to apoptosis. When placed in culture, ileal PP B cells undergo rapid apoptosis. Here, we investigated the expression of the proto-oncogene bcl-2 in ileal PP cells in situ and in culture. Bcl-2 expression has been correlated with the prevention of apoptosis in many cell types. Western blotting, using anti-Bcl-2 monoclonal antibodies, revealed that a Bcl-2-reactive protein of 26,000 MW was expressed in ileal mesenteric lymph node cells, splenocytes and thymocytes from sheep, but was barely detectable in ileal PP B cells in situ or in culture. However, Bcl-2 expression could be markedly induced in ileal PP B cells cultured with phorbol ester and Ca2+ ionophore, a procedure that is known to rescue these cells from apoptosis. We hypothesize that those few B cells that survive a selection event in the ileal PP may begin to express elevated levels of Bcl-2 as they escape from the apoptotic pathway.
journal_name
Immunologyjournal_title
Immunologyauthors
Motyka B,Reynolds JDsubject
Has Abstractpub_date
1995-03-01 00:00:00pages
383-7issue
3eissn
0019-2805issn
1365-2567journal_volume
84pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:Systemic lupus erythematosus (SLE) is an autoimmune disease in which abnormal immune responses are mediated by tissue-binding autoantibodies and immune complex deposition. Because most SLE patients are women of child-bearing age, oestrogen has been suggested to play an important role in SLE pathogenesis. One proposed ...
journal_title：Immunology
pub_type： 临床试验,杂志文章
doi：10.1111/imm.12263
更新日期：2014-08-01 00:00:00
abstract：:A subpopulation of human peripheral blood natural killer (NK) cells, defined by sedimentation at Percoll high buoyant densities (P greater than 1.0635-1.0640 g/ml) and unresponsiveness to interleukin-2 (IL-2), contained two distinct populations based on the intensity of CD16 (FcR gamma III) expression, namely CD16dim ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-01-01 00:00:00
abstract：:The cloning and production of a human anti-rhesus (Rh) D monoclonal antibody (mAb) using the baculovirus-insect cell expression system is described. This monoclonal recombinant antibody R.D7C2 derived from a human parental IgM lambda immunoglobulin was obtained after immortalization of lymphocytes by Epstein-Barr viru...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00219.x
更新日期：1997-05-01 00:00:00
abstract：:Osteopontin (OPN) is important for the function of fibroblasts, macrophages and lymphocytes during inflammation and wound healing. In recent studies of experimental colitis we demonstrated exacerbated tissue destruction in OPN-null mice, associated with reduced tumour necrosis factor-alpha expression and increased mye...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02682.x
更新日期：2007-12-01 00:00:00
abstract：:The levels of IgM, IgA and IgG secreting cells were examined in control, Corynebacterium parvum-stimulated and tumour-bearing, normal and athymic (Nu/Nu) mice. The percentage of IgA to IgM or IgG secreting cells is relatively higher in peripheral blood than in the spleen or peritoneum of normal mice. Within tumours, i...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-05-01 00:00:00
abstract：:Interleukin-2 (IL-2) is a mainstay for current immunotherapeutic protocols but its usefulness in patients is reduced by severe toxicities and because IL-2 facilitates regulatory T (Treg) cell development. IL-21 is a type I cytokine acting as a potent T-cell co-mitogen but less efficient than IL-2 in sustaining T-cell ...
journal_title：Immunology
pub_type： 临床试验,杂志文章
doi：10.1111/imm.12061
更新日期：2013-05-01 00:00:00
abstract：:Guinea-pigs immunized with homogenates of unfed larvae and nymphs of the tick Rhipicephalus appendiculatus developed significant levels of protective immunity to infestation with adults of this species. The mean engorged weight of female ticks feeding on immunized animals (181.96 +/- 05.63 mg and 170.11 +/- 11.54 mg) ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-09-01 00:00:00
abstract：:Previous studies have shown that depletion of Langerhans' cells (LC) from murine epidermis by the superantigen, staphylococcal enterotoxin A (SEA) involves interleukin-1alpha (IL-1alpha) and is inhibitable by agents that block G-protein-associated kinases. The purpose of this study was to determine whether specific ki...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00680.x
更新日期：1999-02-01 00:00:00
abstract：:An examination was made of the effects of eosinophilia on acute inflammation. Sprague Dawley rats were infected with Trichinella spiralis larvae which resulted in a blood eosinophilia. Groups of rats with the induced eosinophilia, and untreated rats without the eosinophilia, were treated with carrageenan, bradykinin o...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-07-01 00:00:00
abstract：:We investigated the function of peripheral blood mononuclear cells (PBMC) in 16 patients with active psoriasis, in 15 patients with static psoriasis and in 27 healthy volunteers, by examining in vitro proliferation and antigen- and mitogen-stimulated production of interleukin-2 (IL-2) and IL-4. Plasma levels of the ne...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:The complement fragment C5a is a potent leucocyte chemoattractant and activator, mediating its effects through a G-protein-coupled receptor. Whilst the C-terminal domain of this receptor has been shown to be essential for receptor desensitization and internalization, it is not known which domains couple to the recepto...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2004.01919.x
更新日期：2004-08-01 00:00:00
abstract：:The studies were designed to investigate the effect of ageing and low protein (4%) diet on antibody-dependent cell-mediated cytotoxicity (ADCC) and IgA concentration in the intestine. Both ADCC activity and IgA concentration were shown to reach mature levels at 17 weeks old. The effect of ageing was greater on ADCC ac...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:Iron-withholding by the chelating agent desferrioxamine abrogates the proliferative response of human peripheral blood mononuclear cells (PBMC) to phytohaemagglutinin (PHA). The present study investigated whether desferrioxamine operates late in the activation process or, as recently suggested, at an early stage, by i...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-10-01 00:00:00
abstract：:We quantified immunoglobulin E (IgE) on peritoneal mast cells of interleukin-4 (IL-4)-gene knockout (-/-) mice and wild-type (+/+) controls using a cytofluorometric method, and examined the expression of IgE receptors, estimated by quantifying the total binding of IgE on the mast cells of IL-4 (-/-) mice. The mast cel...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00727.x
更新日期：1999-04-01 00:00:00
abstract：:The human primary and secondary IgG subclass antibody responses to keyhole limpet haemocyanin (KLH) have been measured by ELISA using IgG subclass-specific monoclonal antibodies. KLH-specific IgG1 and IgG2 antibodies were detected 3 weeks after primary immunization, and IgG1, IgG2 and IgG4 antibodies after secondary i...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-03-01 00:00:00
abstract：:Changes in B-cell phenotype during development of ileal and jejunal Peyer's patches (PP) of sheep were investigated using flow cytometry and immunoperoxidase-stained cryosections. On Day 104 of gestation (term at 150 days) B-cell clusters were identified in the lamina propria of the ileum. These clusters were composed...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:Dendritic cells (DC) are the initiators of immune responses and are present in most tissues in vivo. To generate myeloid DC from monocytes (MoDC) in vitro the necessary cytokines are granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Using degenerated primers delineated from other spec...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2005.02319.x
更新日期：2006-04-01 00:00:00
abstract：:The role of oral-associated lymphoid tissues during induction of oral tolerance still remains elusive. Therefore, the aim was to compare T-cell activation and induction of tolerance to ovalbumin (OVA) presented through either of two routes; deposited into the oral cavity, or the stomach, thereby bypassing the oral cav...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12633
更新日期：2016-09-01 00:00:00
abstract：:Purified human and murine monocytes can be activated in vitro by human recombinant IL-2 to secrete tumour necrosis factor (TNF). TNF mRNA is detected within 4 h of addition of IL-2. Signal transduction does not appear to require expression of the Tac peptide of the IL-2 receptor. IL-2 does, however, induce Tac mRNA an...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-08-01 00:00:00
abstract：:Macrophage (MΦ) polarization is triggered during the innate immune response to defend against microbial pathogens, but can also contribute to disease pathogenesis. In a previous study, we found that interleukin-15 (IL-15) -derived classically activated macrophages (M1 MΦ) have enhanced antimicrobial activity, whereas ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.13013
更新日期：2019-02-01 00:00:00
abstract：:Immunization of mice with cognate cancer-derived heat-shock protein (hsp) preparations leads to protection from cancer growth. As hsp used for vaccination or therapy are derived from autologous cancers, questions of pathological autoimmunity are of immense significance for the ongoing translation of this approach to t...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00127.x
更新日期：2000-11-01 00:00:00
abstract：:The immunosuppression exhibited by a water-soluble condensate of tobacco smoke (WSC) has been studied in vivo and in vitro. When multiple sublethal doses of WSC were injected into C57Bl/6 mice, their ability to respond to immunization with sheep erythrocytes by the formation of plaque-forming cells was severely inhibi...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-08-01 00:00:00
abstract：:Histamine not only mediates immediate allergic reactions, it also regulates cellular immune responses. H4R is the most recently identified histamine receptor (HR). In the present study, we examined the in vitro effect of histamine and H4R agonists on the responses of human T cells to purified protein derivative from M...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02574.x
更新日期：2007-06-01 00:00:00
abstract：:Incubation of sensitized mononuclear cells from patients with schistosomiasis with specific antigen containing a suspension of viable Schistosoma haematobium ova resulted in the release of a soluble factor which was chemotactic for eosinophils. Production of this substance was detectable at 24 h and demonstrated peak ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:Montelukast is a leukotriene receptor antagonist that is known to prevent allergic rhinitis and asthma. Blocking the Cysteinyl leukotrienes receptor (CysLTR1), one of the primary receptors of leukotrienes, has been demonstrated to be efficacious in ameliorating experimental autoimmune encephalomyelitis (EAE), an anima...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.13308
更新日期：2021-01-22 00:00:00
abstract：:Natural killer (NK) cells have a specialized function in peripheral organs, which is determined by the organ-specific niches. We have attempted to explore whether lung NK cells display a particular phenotype according to their function in the unique pulmonary environment in health or during respiratory infection in mi...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2012.03607.x
更新日期：2012-09-01 00:00:00
abstract：:The cellular prion protein (PrPc) is a glycosyl-phosphatidylinositol (GPI)-linked cell surface protein, which is expressed at high density on nervous tissues and at lower levels on most other solid-organ tissues. It is also expressed on peripheral blood mononuclear cells (PBMC) of all lineages. In lymphocytes, its lev...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2001.01166.x
更新日期：2001-02-01 00:00:00
abstract：:The expression of adhesion molecules on vascular endothelial cells determines the pattern of migration and extravasation of leucocytes in inflammation and immunity. Here we show that costimulation with CD40 ligand (CD40L) and interleukin (IL)-4 (or IL-13) gives rise to a unique pattern of adhesion molecule expression ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00061.x
更新日期：2000-08-01 00:00:00
abstract：:It is believed that self-reactive forbidden T-cell clones are generated by 'failure' of the pathway of T-cell differentiation in the thymus, if it is disturbed. We examined how such forbidden clones are generated under immunosuppressive conditions. Mice were treated with an injection of deoxyspergualin, FK506, or cycl...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00222.x
更新日期：1997-05-01 00:00:00
abstract：:Tumour necrosis factor-alpha (TNF-alpha) and cis-urocanic acid (UCA) have recently been implicated in the process by which ultraviolet B radiation (UVB) impairs the induction of contact hypersensitivity when dinitrofluorobenzene (DNFB) is painted on UVB-exposed skin. The evidence supports the hypothesis that UVB radia...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-05-01 00:00:00
