Abstract:
:Deficiency in gammadelta T cells aggravates colitis in animal models suggesting that gammadelta T cells have regulatory properties. Therefore, proliferation, suppression and cytokine secretion of human gammadelta T cells were determined in vitro. Human peripheral gammadelta T cells were isolated from the whole blood of healthy donors by magnetic antibody cell sorting technology. The proliferation after CD3/CD28 stimulation was measured by (3)[H]thymidine incorporation. Interferon-gamma (IFN-gamma), interleukin-2 (IL-2), transforming growth factor-beta (TGF-beta) and IL-10 concentrations were measured by enzyme-linked immunosorbent assay; TGF-beta messenger RNA was also measured by reverse transcription-polymerase chain reaction. The expression of latency associated peptide (LAP), a TGF-beta complex component, intracellular cytokine content and T helper cell proliferation were measured by flow cytometry. Human gammadelta T cells showed poor proliferation upon CD3/CD28 stimulation and suppressed T helper cell growth stronger than CD4(+) CD25(+) T cells, although gammadelta T cells were FOXP3 negative. They secreted little IL-2 but high concentrations of IFN-gamma, IL-10 and TGF-beta. When looking at LAP expression the Vdelta1 subset was found to be the main TGF-beta producer compared to Vdelta2 T cells. Taken together, peripheral gammadelta T cells have in vitro a more potent regulatory potential than CD4(+) CD25(+) cells regarding T helper cell suppression. This is most likely the result of strong TGF-beta secretion, particularly by the Vdelta1 subset.
journal_name
Immunologyjournal_title
Immunologyauthors
Kühl AA,Pawlowski NN,Grollich K,Blessenohl M,Westermann J,Zeitz M,Loddenkemper C,Hoffmann JCdoi
10.1111/j.1365-2567.2009.03162.xsubject
Has Abstractpub_date
2009-12-01 00:00:00pages
580-8issue
4eissn
0019-2805issn
1365-2567pii
IMM3162journal_volume
128pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::The process by which naive T cells become activated, differentiate into effector cells and ultimately generate long-lived memory cells is dependent upon a number of factors, including the costimulatory signals received by the T cell. To best understand the multiple events involved, it is important to understand the po...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02396.x
更新日期:2006-08-01 00:00:00
abstract::The humoral and mucosal immune responses to oral immunization with xenogeneic antibodies were studied using an animal model in which female rabbits were fed daily doses of the MOPC-315 murine IgA antibody, and were mated during the course of the feeding programme. Serum and colostrum samples were assayed for the prese...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-08-01 00:00:00
abstract::Mice injected at birth with semi-allogeneic lymphoid cells develop a lupus-like autoimmune syndrome in which donor B cells are polyclonally activated by host alloreactive CD4+ T cells, producing autoantibodies and immune complex-mediated glomerulonephritis. It has been demonstrated that the recognition of major histoc...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Antiserum specific for hamster thymus-derived lymphocytes, prepared by immunization of rabbits with brain tissue from MHA/ssLAK hamsters, was, in the presence of guinea-pig complement, cytotoxic to hamster thymocytes greater than lymph node cells greater than spleen cells, while virtually unreactive against bone marro...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-07-01 00:00:00
abstract::A murine anti-B monoclonal antibody was obtained by the hybridoma technique. This antibody called anti-B (b-183) is of IgM nature; it is capable of agglutinating normal B, B3, Bx, cis AB and some acquired B red cells. Its association constant is 1.1 X 10(8) l/mol, and appears high compared to those of the monoclonal a...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::The aim of this study is to investigate the clinical significance of the ratio between interleukin-17 (IL-17) secreting cell and FOXP3-positive regulatory T cell (FOXP3(+) Treg) infiltration in renal allograft tissues with acute T-cell-mediated rejection (ATCMR). Fifty-six patients with biopsy-proven ATCMR were includ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2012.03588.x
更新日期:2012-07-01 00:00:00
abstract::Mice were primed with TNP-derivatized insulin, or TNP-Mycobacteria, and lymph node cells were challenged in vitro with haptenated and unhaptenated antigens. Using either priming antigen, T-cell proliferative responses could be obtained to TNP-insulin. In B10 (H-2b), mice, which are responders to beef insulin (BI), but...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Thioglycollate-stimulated, adherence and density gradient-enriched peritoneal exudate cells were used for the preparation of a specific, highly active antimacrophage reagent. After absorption with mouse lymphoid cells, an antimacrophage globulin (AMG) fraction was shown to be cytotoxic for peritoneal exudate macrophag...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::Studies of tuberculosis have suggested a shift in dominance from a T helper type 1 (Th1) towards a Th2 immune response that is associated with suppressed cell-mediated immune (CMI) responses and increased humoral responses as the disease progresses. In this study a natural host disease model was used to investigate th...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2004.02003.x
更新日期:2005-01-01 00:00:00
abstract::Factors that influence the generation and maintenance of memory CD8+ T cells are not fully understood. The homeostasis of memory T cells is highly dynamic and tightly regulated by various stimuli, including cytokines and antigen-major histocompatibility complex ligands. We characterized the hepatitis C virus (HCV)-spe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2003.01769.x
更新日期:2003-12-01 00:00:00
abstract::Free malarial merozoites isolated from in vitro cultures of P. knowlesi and emulsified with Freund's complete (FCA) or incomplete (FIA) adjuvant were used to vaccinate twelve Rhesus monkeys against the uniformly lethal infection caused by P. knowlesi. Initial challenge of six monkeys with the same parasite variant as ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::The studies were designed to investigate the effect of ageing and low protein (4%) diet on antibody-dependent cell-mediated cytotoxicity (ADCC) and IgA concentration in the intestine. Both ADCC activity and IgA concentration were shown to reach mature levels at 17 weeks old. The effect of ageing was greater on ADCC ac...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::Nod-like receptors are a family of innate immune receptors that link cytosolic sensing of microbial and danger stimuli to the activation of immune responses. Two Nod-like receptor family members, Nod1 and Nod2, recognize bacterial peptidoglycan and activate immune responses via nuclear factor-κB (NF-κB) and mitogen-ac...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12307
更新日期:2014-10-01 00:00:00
abstract::The functional avidity of a cytotoxic T lymphocyte (CTL) is known to be a critical determinant of the efficacy with which it clears pathogens. High avidity cells, which are by definition highly sensitive to peptide antigen, are superior for elimination of viruses and tumours. Our studies have established the ability o...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2011.03440.x
更新日期:2011-07-01 00:00:00
abstract::Attempts were made to break the tolerance of lymph node B cells to deaggregated human gamma globulin. Using allotype-congenic mice, lymph node cells from virgin or tolerant (5 mg) CBA/Igb donors were transferred tonormal CBA/Iga recipients and the proportion of responding donor B cells estimated 1 and 13 days later. T...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1977-11-01 00:00:00
abstract::Mouse lymph node cells cultured with Concanavalin A (Con A) in serum-free medium containing 59Fe-transferrin took up 59Fe more rapidly than cells cultured without Con-A. Uptake of iron commenced rapidly and preceded the onset of DNA synthesis in stimulated cells. Total uptake of transferrin during culture was much low...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::The influence of antigens of the major histocompatibility complex (MHC) on the production of interferon (IFN) alpha or IFN gamma by human peripheral blood leucocytes (PBL) in vitro has been studied. Synthesis of IFN gamma by PBL stimulated with purified phytohaemagglutinin (PHA-P) or protein A of Staphylococcus aureus...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-06-01 00:00:00
abstract::The non-specific normal immunosuppressive protein (Nip) has been described in our laboratory and its biological activity was extensively studied. In the present study, further purification analyses of Nip were conducted. Fractionation of Nip by Ultrogel AcA 34 column resulted in peak (I) that displayed Nip activity in...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::The Ig subunit structure of murine B lymphocytes was studied by employing different radiolabelling techniques in combination with chemical cross-linking. The main membrane structure of IgM was a half molecule that was disulphide-linked to proteins with MW 30,000, 45,000 and 55,000, respectively. Small amounts of mu 2L...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-06-01 00:00:00
abstract::MUC1 is a transmembrane mucin that is expressed on ductal epithelial cells and epithelial malignancies and has been proposed as a target antigen for immunotherapy. The expression of MUC1 has recently been reported on T and B cells. In this study we demonstrate that following activation in vivo or activation by differe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01562.x
更新日期:2003-01-01 00:00:00
abstract::The generation of effective immunity requires that antigen-specific T cells are activated, clonally expanded and ultimately eliminated by apoptosis. The involvement of CD95-mediated apoptosis in T-cell elimination is well established, but the conditions which regulate the death pathway under normal circumstances are s...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00925.x
更新日期:1999-12-01 00:00:00
abstract::Bacillus Calmette-Guérin (BCG) inoculation has been shown to inhibit certain immune functions. To determine whether this inhibition adversely affects host defences against infection, the effect of BCG on Listeria infection in mice was investigated. Mice were injected intravenously (i.v.) with Listeria monocytogenes an...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-11-01 00:00:00
abstract::Four types of lymphocyte chemotactic factor (LCF-a, -b, -c and -d) could be isolated from extract of 24-hr-old delayed-type hypersensitivity (DTH) skin reaction sites induced with purified protein derivative (PPD) in guinea-pigs by gel filtration on Sephadex G-100 followed by chromatography with DEAE-Sephadex. Partial...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract::Among peripheral T cells, the expression of CD4 and CD8 is almost mutually exclusive. However, here we show, using flow cytometric analysis, that ex vivo approximately 6% of rat T cells stained for both CD4 and CD8. These double positive cells were also detected by confocal microscopy. Only around 50% of double positi...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00071.x
更新日期:2000-10-01 00:00:00
abstract::To date, the most potent mucosal vaccine adjuvants to be identified have been bacterial toxins. The present data demonstrate that the type 2 ribosome-inactivating protein (type 2 RIP), mistletoe lectin I (ML-I) is a strong mucosal adjuvant of plant origin. A number of plant lectins were investigated as intranasal (i.n...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01157.x
更新日期:2001-01-01 00:00:00
abstract::The present study aimed to determine whether the frequency of double positive (DP) thymocytes expressing alphabeta T-cell receptor (TCR) clonotypes at the time of selection regulates peripheral CD4 T-cell compartment size. Scid recipients were inoculated with various ratios of TCR Calpha(0/0) and wild-type bone marrow...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02240.x
更新日期:2005-11-01 00:00:00
abstract::The specific recognition of the acetylcholine receptor and its alpha-subunit by T cells derived from patients with myasthenia gravis or mice with experimental autoimmune myasthenia gravis raises the question of the role of autoreactive T cells in the myasthenic process. Sequences of the acetylcholine receptor alpha-su...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1990-04-01 00:00:00
abstract::Aspergillus fumigatus is often associated in asthmatic patients with the exacerbation of asthma symptoms. The pathomechanism of this phenomenon has not been fully understood. Here, we evaluated the immunological mechanisms and the role of the prostaglandin D2 / Chemoattractant Receptor-Homologous Molecule Expressed on...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.12234
更新日期:2014-05-01 00:00:00
abstract::Protectin (CD59) is a glycolipid-anchored inhibitor of the membrane attack complex (MAC) of human complement (C) that protects blood cells, endothelial cells and various epithelial cells from C-mediated lysis. Because of its activities protectin is a candidate molecule for use in the treatment of paroxysmal nocturnal ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-07-01 00:00:00
abstract::This study addresses the question of whether human peripheral CD4+ CD45RA+ T cells possess antigen-specific immune memory. CD4+ CD45RA+ T cells were isolated by a combination of positive and negative selection. Putative CD4+ CD45RA+ cells expressed CD45RA (98.9%) and contained < 0.1% CD4+ CD45RO+ and < 0.5% CD4+ CD45R...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00274.x
更新日期:1997-07-01 00:00:00