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Phenotypic analysis of peripheral CD4+ CD8+ T cells in the rat.

Abstract：

:Among peripheral T cells, the expression of CD4 and CD8 is almost mutually exclusive. However, here we show, using flow cytometric analysis, that ex vivo approximately 6% of rat T cells stained for both CD4 and CD8. These double positive cells were also detected by confocal microscopy. Only around 50% of double positive cells expressed the CD8beta chain, the remaining cells expressed the CD8alpha chain alone. Double positive cells were blast-like with a phenotype, distinct from that of either CD4 or CD8 single positive cells, suggestive of an activated state. Previous reports of double positive T cells have also suggested that coexpression of CD4 and CD8 is linked to the activation state of the cell. There was an indication that priming animals with a hapten-carrier complex increased the ratio of CD8alphaalpha : alphabeta expressing double positive T cells, although we did not detect an increase in the frequency of double positive T cells following priming. We also show that the frequency of double positive cells was reduced following thymectomy and with age. In conclusion, these studies show that peripheral T cells expressing both CD4 and CD8 can be detected in the rat and that they are phenotypically distinct from CD4 and CD8 single positive T cells.

journal_name

Immunology

journal_title

Immunology

authors

Kenny E,Mason D,Pombo A,Ramírez F

doi

10.1046/j.1365-2567.2000.00071.x

keywords:

subject

Has Abstract

pub_date

2000-10-01 00:00:00

pages

178-84

issue

2

eissn

0019-2805

issn

1365-2567

pii

imm071

journal_volume

101

pub_type

杂志文章

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