Abstract：
:Polyclonal rabbit anti-idiotypic (Ab2) antibodies raised against the antiprogesterone mAb DB3 (Ab1) were used to induce an Ab3 antiprogesterone response in BALB/c mice. While the affinity of Ab3 sera for progesterone was 10-50-times lower than that of DB3, their steroid-binding specificity showed considerable similarity to DB3. Two immunoglobulin M (IgM) Ab3 monoclonal antibodies (mAbs), 1A4 and 3B11, were obtained, both of which bound progesterone conjugated to bovine serum albumin (progesterone-BSA). 1A4 also bound free progesterone, although with low affinity and very broad cross-reactivity. Like DB3, 1A4 is encoded by a heavy-chain variable region (VH) gene segment from the small VGAM3.8 family, a restriction that is characteristic of antibodies raised against progesterone-11alpha-BSA. In contrast, 3B11 binds progesterone-11alpha-BSA but not free progesterone and is encoded by an unrelated VH gene from the J558 family. The light chain variable region (VL) of 1A4 lacks the intradomain disulphide bridge owing to replacement of CysL23 by Tyr. Both the 1A4 and 3B11 heavy chains have extremely short complementarity determining region (CDR) H3 loops, comprising three and four amino acids, respectively. Modelling of the combining site of 1A4 from the X-ray crystallographic structure of DB3 indicates that the short H3 loop is a major factor in the loss of affinity and specificity for steroid.
journal_name
Immunologyjournal_title
Immunologyauthors
Kirsch RD,Beale D,He M,Corper AL,Krawinkel-Brenig U,Taussig MJdoi
10.1046/j.1365-2567.2000.00006.xkeywords:
subject
Has Abstractpub_date
2000-06-01 00:00:00pages
152-64issue
2eissn
0019-2805issn
1365-2567pii
imm006journal_volume
100pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:Previous studies have highlighted the importance of CD4+ T cells in regulation of IgA responses and have indicated a functional heterogeneity among these cells between inductive (Peyer's patch) and effector (lamina propria) sites in the intestine. To determine whether these functional differences could be accounted fo...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-12-01 00:00:00
abstract：:Chemokine-mediated signalling involves the activation of a Janus kinase (Jak) pathway. We have previously shown that Jak3 mediates CCR9 and CXCR4 signalling in response to CCL25 and CXCL12 in BM progenitors and thymocytes. The lack of peripheral lymph nodes and Peyer's patches observed in Jak3(-/-) mice suggested a po...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02634.x
更新日期：2007-10-01 00:00:00
abstract：:Contact sensitization by epicutaneous application of picryl chloride causes in mice a significant reduction of the antibody responses to immunogenic TNP-conjugates. This split unresponsiveness is along-lasting. It was found that hapten applied on the skin became attached to the serum proteins and the transfer of such ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-08-01 00:00:00
abstract：:The epigenetic regulation of transcription factor genes is critical for T-cell lineage specification. A specific methylation pattern within a conserved region of the lineage specifying transcription factor gene FOXP3, the Treg-specific demethylated region (TSDR), is restricted to regulatory T (Treg) cells and is requi...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12261
更新日期：2014-07-01 00:00:00
abstract：:Mice simultaneously injected intravenously and subcutaneously with herpes simplex virus fail to adoptively transfer delayed hypersensitivity (DH) to syngeneic recipients. The transferred lymph node cells also failed to rapidly eliminate infectious herpes from the pinna, despite the presence of cytotoxic T cells in the...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-04-01 00:00:00
abstract：:In the present paper it is shown that the non-species specific determinant of the antigenic thymus-brain system previously detected in murine and human brain also exists in human thymus. However, in contrast to the situation in mice and rats this antigenic structure is not expressed on suspended thymic lymphocytes, bu...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
abstract：:In the study described here, the effect of human tears and purified lactoferrin was investigated on the deposition of complement components on solid-phase bound immune complexes. After incubating immune complexes with fresh normal human serum, the deposition of complement components (C3, C4 and C5) was measured with a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
abstract：:Free fatty acids (FFA) were tested for their effects on in vitro thymus-dependent (TD) and thymus-independent (TI) immune responses. Murine T cells proliferating in one-way mixed leucocyte reactions (MLR) were extremely sensitive to inhibition by exogenous stearic acid (18:0) but were only moderately affected by oleic...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-12-01 00:00:00
abstract：:Cells subjected to nucleoside incorporation studies using radiolabelled materials may suffer radiation damage that can alter the results. We did scintimetric and cytofluorographic analysis to confirm this and to determine the optimal experimental doses of, and exposure times to, [3H]-TdR, in order to prevent or minimi...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-12-01 00:00:00
abstract：:Lipoprotein lipase (LPL) is the key enzyme in the metabolism of triglyceride-rich lipoproteins. The patterns of LPL mRNA expression and secretion of the enzyme have not yet been established in natural killer (NK) cells. We show in the present communication that CD3- CD16+ cells (NK cells) transcribe LPL mRNA, express ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-10-01 00:00:00
abstract：:Rabbit IgG and its Fab, Fc and pFc' fragments, prepared by papain or peptic digestion, were assayed for binding to homologous peritoneal macrophages. The binding affinity of IgG for the peritoneal macrophages (Ka = 5.9 +/- 1.6 x 10(5) L/M) was comparable to that recorded with alveolar macrophages (7.6 +/- 1.8 x 10(5) ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-03-01 00:00:00
abstract：:Compared with non-transgenic controls, mice bearing an Fv4 murine retroviral env transgene resist infection and do not become immunosuppressed when inoculated with Friend virus (FV). When immunized with FV antigens in the absence of infectious virus, they make antibodies and cytotoxic lymphocytes (CTL) to FV comparabl...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00157.x
更新日期：1997-02-01 00:00:00
abstract：:Immunohistological techniques have been used to study the stromal cells of the human placenta in both the chorionic villous mesenchyme and the connective tissue underlying the amnion. Throughout gestation many of these cells express an antigen (3C10) that is found on mononuclear phagocytes but not on dendritic cells o...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-05-01 00:00:00
abstract：:MRL/Mp-lpr/lpr (lpr/lpr) mice but not the congenic MRL/Mp-+/+ (+/+) mice, develop a generalized lymph node (LN) hypertrophy reflecting the expansion of a T-cell population that acts as an enhancing factor for autoimmunity. In order to characterize better this T-cell population, we investigated some of its surface prop...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:Several functional parameters and surface markers were studied serially in rnu/rnu rats and compared to rnu/+ controls. Expressed as a percentage of labelled splenocytes, rnu/rnu animals had decreased W3/13 (P less than 0.01) and W3/25 (P less than 0.01) positive cells. Both W3/13 and W3/25 antigens peaked at Week 16 ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-08-01 00:00:00
abstract：:Degradation of the extracellular matrix plays an important role in rheumatoid articular destruction. Rheumatoid synovial fibroblasts secrete a large amount of matrix-degrading metalloproteinases (MMPs), which initiate tissue damage by proteolytic degradation of collagens and proteoglycans. Cytokines, such as interleuk...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1996.d01-789.x
更新日期：1996-12-01 00:00:00
abstract：:Humans and other mammals coexist with a diverse array of microbes colonizing the intestine, termed the microflora. The relationship is symbiotic, with the microbes benefiting from a stable environment and nutrient supply, and the host gaining competitive exclusion of pathogens and continuously maintenance of the gut i...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02687.x
更新日期：2008-02-01 00:00:00
abstract：:Cutaneous delayed hypersensitivity reactions to collagen in guinea-pigs were partially but specifically suppressed if the animals had been pretreated with collagen and Freund's incomplete adjuvant. Such animals responded normally to skin-reactive factor prepared with ovalbumin. Lymphoid cells from animals with normal ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1976-07-01 00:00:00
abstract：:Previous studies have shown that depletion of Langerhans' cells (LC) from murine epidermis by the superantigen, staphylococcal enterotoxin A (SEA) involves interleukin-1alpha (IL-1alpha) and is inhibitable by agents that block G-protein-associated kinases. The purpose of this study was to determine whether specific ki...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00680.x
更新日期：1999-02-01 00:00:00
abstract：:The linker protein LAT is expressed mainly in T and natural killer (NK) cells. LAT-deficient mice have an arrest of intrathymic T-cell development at the CD4+ CD8+ stage and lack mature T cells in the periphery. However, no gross abnormality in development and function of the B and NK cells has been described. Here we...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2003.01671.x
更新日期：2003-07-01 00:00:00
abstract：:Tuberculin-active peptide-reactive T-cell lines were established from the popliteal lymph node of BALB/cA mice immunized with heat-killed Mycobacterium tuberculosis to investigate the cellular mechanisms in the protective immunity of tuberculosis. These T-cell lines, consisting mainly of L3T4 surface antigen-positive ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
abstract：:Adoptive transfer of T-cells is a promising therapy for many cancers. To enhance tumour recognition by T-cells, chimeric antigen receptors (CARs) consisting of signalling domains fused to receptors that recognize tumour-associated antigens can be expressed in T-cells. While CAR T-cells have shown clinical success for ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.13187
更新日期：2020-07-01 00:00:00
abstract：:In the past 15 years, regulatory T cell (Treg) suppression has graduated from a phenomenon that 'dare not speak its name' to a field at the centre of a global research effort. It is now accepted that Tregs can target numerous cell populations to elicit potent immunosuppression. Intriguingly, emerging data suggest that...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2009.03053.x
更新日期：2009-04-01 00:00:00
abstract：:Regeneration of the thymus was studied in rats that were treated with a single oral dose of the organotin compound di-n-butyltin dichloride (DBTC). After an initial maximum depletion of cortical BrdU+ thymocytes on day 2 after treatment, repopulation appeared to start on day 3 as indicated by an increased number of Br...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:The defined antigen substrate spheres (DASS) system was employed for analysing reactions between solid state antibody or antigen and soluble immune complexes. Sepharose beads covalently coupled with ovalbumin were used to represent tumour cells and beads coupled with antibody against ovalbumin were used to represent a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-06-01 00:00:00
abstract：:Examples suggesting that all or part of the V(H) segment of a rearranged V(H)DJ(H) may be replaced by all or part of another V(H) have been appearing since the 1980s. Evidence has been presented of two rather different types of replacement. One of these has gained acceptance and has now been clearly demonstrated to oc...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2567.2004.02084.x
更新日期：2005-02-01 00:00:00
abstract：:It has previously been shown in mice that activated peritoneal macrophages, when first removed from the peritoneal cavity, suppress mitogen-stimulated lymphocyte transformation in vitro as measured by [3H]-thymidine incorporation. In this study, it is demonstrated that activated macrophages that have been incubated al...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-06-01 00:00:00
abstract：:To determine the influence of anti-idiotypic antibodies on the complementary idiotype (Id), the clearance kinetics, tissue distribution and fate of idiotype-anti-idiotype complexes were investigated in mice. The complexes were prepared by mixing purified radiolabelled monomeric (mIgA) or polymeric IgA (pIgA) with mono...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-04-01 00:00:00
abstract：:Mice of the strains A.TH and A.TL were rendered neonatally tolerant to class II major histocompatibility complex (MHC) by the injection of (A.TH x A.TL)F1 spleen and bone marrow cells within 24 hr of birth. Spleen and thymus cells from adult tolerant mice bearing long-term surviving skin grafts were compared with thos...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:In order to explore the role of glutathione in cell-mediated cytotoxicity, we have examined the effect of the sulphydryl-reactive and glutathione-depleting agent 2-cyclohexene-1-one on antibody-dependent cellular cytotoxicity, spontaneous cell-mediated cytotoxicity, and cell-mediated lympholysis by human peripheral bl...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
