Abstract：
:The number, distribution and surface phenotype of dividing cells in the thymus, and differences between the cell cycle status of thymocyte subpopulations, were studied in fetal and post-natal lambs using double-labelling techniques. Dividing cells were labelled in vivo for various periods with 5-bromo-2-deoxyuridine (BrdU). The proportions of constituent thymocyte subpopulations that had synthesized DNA during the labelling period were measured by flow cytometry or immunohistochemistry using a panel of monoclonal antibodies (mAbs) specific for sheep lymphocyte differentiation antigens and MHC class I and class II antigens in conjunction with an anti-BrdU mAb. The proportion of thymocytes that incorporated BrdU during a 1-hr labelling period varied with age, and levels of 30%, 13% and 9% were measured, respectively, in 40- and 125-day-old fetuses and 8-week-old lambs. Eight percent of the thymocytes in lambs were synthesizing DNA, with 4% entering the G2 phase per hour, and a substantial number of thymocytes (21%) had a G2 + M phase DNA content. A small subset of thymocytes (1-3%) recognized by mAb E-79 localized to the subcapsular region of the cortex and displayed the highest level of BrdU incorporation. Cortical-type thymocytes (CD1+) comprised 50-70% of thymocytes; however, few of these incorporated BrdU and the proportion in the G2 + M phase of the cell cycle was higher than for other thymocyte subpopulations. The 197+CD4-CD8- T cells also showed no evidence of in vivo division.
journal_name
Immunologyjournal_title
Immunologyauthors
McClure S,Dudler L,Thorpe D,Hein WRsubject
Has Abstractpub_date
1988-11-01 00:00:00pages
393-9issue
3eissn
0019-2805issn
1365-2567journal_volume
65pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:Staphylococcus aureus is a major pathogen that can cause a broad spectrum of serious infections including skin infections, pneumonia and sepsis. Peritoneal mast cells have been implicated in the host response towards various bacterial insults and to provide mechanistic insight into the role of mast cells in intraperit...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12297
更新日期：2014-10-01 00:00:00
abstract：:Donor T cells are crucial for target organ injury in graft-versus-host disease (GVHD). We examined the effects of donor T cells on the target organs using a parent-into-F1 model of acute and chronic GVHD. Donor T cells showed engraftment in the spleen, small intestine and liver of mice with acute GVHD, causing typical...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2001.01240.x
更新日期：2001-07-01 00:00:00
abstract：:Thoracic duct lymphocytes (TDL) from (AS2 x AS)F1 rats previously injected with human serum albumin (HSA) were transferred to 900 r irradiated syngeneic recipients which were challenged with various doses of soluble HSA (s-HSA). TDL from partially tolerant rats, which were deficient in high avidity B cells, produced a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1977-10-01 00:00:00
abstract：:The effect of corticosteroid administration on the redistribution of sirculating lymphocytes was studied in the guinea-pig, since this species closely resembles man in its relative resistance to the lymphopenic effect of corticosteroids. A single intravenous injection of hydrocortisone (either 10 mg or 100 mg/kg) cau...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-04-01 00:00:00
abstract：:The uptake by human blood monocytes of sheep erythrocytes treated with rabbit anti-sheep antibody with or without mouse complement was assessed by a radioactive method to discover whether immune complexes would inhibit this reaction. It was found that sera from SLE patients inhibited uptake whereas normal sera enhance...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:FTY720, a novel immunosuppressant, sequesters circulating mature lymphocytes, especially T cells, within lymph nodes and Peyer's patches by accelerating lymphocyte homing, and thereby causes lymphocyte depletion in the blood. The FTY720-induced acceleration of lymphocyte homing appears to be mediated by lymphocyte hom...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1998.00639.x
更新日期：1998-12-01 00:00:00
abstract：:Natural infection by mouse hepatitis virus (MHV) can affect interpretation of immunological studies in mice. MHV, a collective term describing a group of corona viruses, is found in natural infections in over 70% of laboratory mouse populations in the U.S.A. and Canada. Natural outbreaks of MHV in our animal colony af...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:Trypanosoma cruzi, the aetiological agent of Chagas disease, has a highly efficient detoxification system to deal with the oxidative burst imposed by its host. One of the antioxidant enzymes involved is the cytosolic tryparedoxin peroxidase (c-TXNPx), which catalyses the reduction to hydrogen peroxide, small-chain org...
journal_title：Immunology
pub_type： 临床试验,杂志文章
doi：10.1111/imm.12979
更新日期：2018-11-01 00:00:00
abstract：:The Ig subunit structure of murine B lymphocytes was studied by employing different radiolabelling techniques in combination with chemical cross-linking. The main membrane structure of IgM was a half molecule that was disulphide-linked to proteins with MW 30,000, 45,000 and 55,000, respectively. Small amounts of mu 2L...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-06-01 00:00:00
abstract：:Adrenaline is a catecholamine hormone secreted by the adrenal medulla in response to acute stress. Previous studies have shown that adrenaline suppresses the nitric oxide (NO) response of murine macrophages (M phi s) stimulated in vitro with lipopolysaccharide (LPS). We have now extended these studies to examine the e...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2001.01332.x
更新日期：2001-12-01 00:00:00
abstract：:Factors that influence the generation and maintenance of memory CD8+ T cells are not fully understood. The homeostasis of memory T cells is highly dynamic and tightly regulated by various stimuli, including cytokines and antigen-major histocompatibility complex ligands. We characterized the hepatitis C virus (HCV)-spe...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2003.01769.x
更新日期：2003-12-01 00:00:00
abstract：:Syngeneic (CB20) thymus and spleen T cells transferred to SCID mice, and shown to be present by flow cytometric analysis and functional by their ability to reject third-party skin grafts, do not lead to a detectable failure of syngeneic or allogeneic pregnancy. Unless the small number of B cells contaminating the T-ce...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:Leukosialin or CD43 is a heavily O-glycosylated transmembrane protein expressed on all cells of the haematopoietic cell lineage with the exception of red blood cells and mature B cells. This antigen has been identified in human, mouse and rat with monoclonal antibodies. Although orthologues of many human and rodent le...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00604.x
更新日期：1999-01-01 00:00:00
abstract：:Natural killer (NK) cells have a specialized function in peripheral organs, which is determined by the organ-specific niches. We have attempted to explore whether lung NK cells display a particular phenotype according to their function in the unique pulmonary environment in health or during respiratory infection in mi...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2012.03607.x
更新日期：2012-09-01 00:00:00
abstract：:MUC1 is a transmembrane mucin that is expressed on ductal epithelial cells and epithelial malignancies and has been proposed as a target antigen for immunotherapy. The expression of MUC1 has recently been reported on T and B cells. In this study we demonstrate that following activation in vivo or activation by differe...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2003.01562.x
更新日期：2003-01-01 00:00:00
abstract：:The purpose of this study was to investigate the effect of cyclophosphamide (CY) and 6-mercaptopurine (6-MP) on the production of the two IgG antibody subclasses in guinea-pigs. Following the intradermal application of dinitrophenylated bovine gamma globulin (DNP20-BGG) emulsified in Freund's incomplete adjuvant (FIA)...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
abstract：:Mouse peritoneal macrophages (M phi) expressed enhanced tumoricidal activity upon in vitro stimulation either with the lymphokine M phi-activating factor (MAF) or with fibroblast interferon (IFN-beta). In contrast, M phi suppressive activity on lymphoproliferation was not affected by MAF pretreatment, but was drastica...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1983-11-01 00:00:00
abstract：:Degradation of the extracellular matrix plays an important role in rheumatoid articular destruction. Rheumatoid synovial fibroblasts secrete a large amount of matrix-degrading metalloproteinases (MMPs), which initiate tissue damage by proteolytic degradation of collagens and proteoglycans. Cytokines, such as interleuk...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1996.d01-789.x
更新日期：1996-12-01 00:00:00
abstract：:A suppressive B-cell factor (SBF)-producing hybridoma termed TS-4.44 was established by fusion of B cells which possessed receptors for the Fc portion of IgG (FcR gamma + B cells) and thymidine kinase defective fibroblasts, 3T3-4E cells. The biological properties of hybridoma-produced SBF (Hyb-SBF) are almost the same...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1983-12-01 00:00:00
abstract：:A major role has been recently ascribed to the neutrophil in the resistance to infection by Listeria monocytogenes (L. monocytogenes). Here we evaluated whether such neutrophils played a role in the non-specific resistance to listeriosis that develops in hosts infected by mycobacteria. We found that the depletion of n...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1996.d01-758.x
更新日期：1996-11-01 00:00:00
abstract：:The immunosuppressive effect of cyclosporin A (CS-A) was investigated in RIC-Sprague-Dawley rats. In vivo, CS-A totally abolished the formation of antibodies to the hapten dinitrophenyl (DNP) in rats immunized with DNP-keyhole limpet haemocyanin. In vitro, the effect of CS-A was investigated in spleen cell cultures st...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-04-01 00:00:00
abstract：:Oral administration of antigen induces a state of tolerance that is associated with activation of CD8+ T cells that can transfer unresponsiveness to naïve syngeneic hosts. These T cells are not lytic, but they inhibit development of antibody, CD4+ T helper cell, and CD8+ cytotoxic T lymphocyte (CTL) responses upon ado...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.0019-2805.2003.01793.x
更新日期：2004-02-01 00:00:00
abstract：:In an attempt to produce monoclonal antibodies against human large granular lymphocytes (LGL), the effector cells of natural killer (NK) and killer (K) activity, a monoclonal IgM antibody (VEP10) has been obtained. This antibody is reactive by indirect membrane immunofluorescence (IMF) with 14.7 +/- 8.5% peripheral bl...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1983-06-01 00:00:00
abstract：:The immunological basis for the production of autoantibodies characteristic of systemic lupus erythematosus (SLE) against a wide range of antigens remains obscure. The specificity of (NZB x NZW)F1 (BWF1) or MRL/Mp-lpr/lpr (MRL/lpr) mouse monoclonal antibodies (mAb) was examined by immunofluorescence, immunoblotting an...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-12-01 00:00:00
abstract：:The absence of complement receptor 1 (CR1) related gene/protein y (Crry) leads to embryonic lethality as a result of unrestricted complement activation and concomitant neutrophil infiltration. Here we used Crry(-/-)C3(+/-) mice to investigate the role of Crry in the pathogenesis of immune complex glomerulonephritis (G...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2009.03102.x
更新日期：2009-09-01 00:00:00
abstract：:The in vitro frequency and proliferative capacity of B cells and B-cell precursors in old mice was assessed. The mitogenic response to the B-cell mitogens lipopolysaccharide (LPS) and dextran sulphate decline later and at a slower rate than the T-cell response to phytohaemagglutinin and concanavalin A. The response to...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-06-01 00:00:00
abstract：:T cells do not generally express Fc receptors (FcRs). However, we report here that C8166 cells, a human CD4+ T lymphoblastoid cell line, widely used in research into the human immunodeficiency virus type 1 (HIV-1), expressed CD64 (Fc gamma RI), CD32 (Fc gamma RII), and CD16 (Fc gamma RIII) on the plasma membrane as sh...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00116.x
更新日期：1997-01-01 00:00:00
abstract：:Antigen-induced allergic airway inflammation is mediated by T helper type 2 (Th2) cells and their cytokines, but the mechanism that initiates the Th2 immunity is not fully understood. Recent studies show that basophils play important roles in initiating Th2 immunity in some inflammatory models. Here we explored the ro...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12240
更新日期：2014-06-01 00:00:00
abstract：:Resident glomerular macrophages from normal rats were isolated and grown through long-term cultures in order to obtain confluent cell monolayers. These cells have a secretory function as revealed by the production of a cytokine with a molecular weight similar to interleukin-1 (IL-1). The demonstration that rat glomeru...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-07-01 00:00:00
abstract：:CD4(+) FOXP3(+) regulatory T (Treg) cells constitute a heterogeneous and plastic T-cell lineage that plays a pivotal role in maintaining immune homeostasis and immune tolerance. However, the fate of human Treg cells after loss of FOXP3 expression and the epigenetic mechanisms contributing to such a phenotype switch re...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12198
更新日期：2014-03-01 00:00:00
