当前位置： SCI文献检索 > IMMUNOLOGY期刊下所有文献 > Mast cells are activated by Staphylococcus aureus in vitro but do not influence the outcome of intraperitoneal S. aureus infection in vivo.

Mast cells are activated by Staphylococcus aureus in vitro but do not influence the outcome of intraperitoneal S. aureus infection in vivo.

Abstract：

:Staphylococcus aureus is a major pathogen that can cause a broad spectrum of serious infections including skin infections, pneumonia and sepsis. Peritoneal mast cells have been implicated in the host response towards various bacterial insults and to provide mechanistic insight into the role of mast cells in intraperitoneal bacterial infection we here studied the global effects of S. aureus on mast cell gene expression. After co-culture of peritoneal mast cells with live S. aureus we found by gene array analysis that they up-regulate a number of genes. Many of these corresponded to pro-inflammatory cytokines, including interleukin-3, interleukin-13 and tumour necrosis factor-α. The cytokine induction in response to S. aureus was confirmed by ELISA. To study the role of peritoneal mast cells during in vivo infection with S. aureus we used newly developed Mcpt5-Cre(+) × R-DTA mice in which mast cell deficiency is independent of c-Kit. This is in contrast to previous studies in which an impact of mast cells on bacterial infection has been proposed based on the use of mice whose mast cell deficiency is a consequence of defective c-Kit signalling. Staphylococcus aureus was injected intraperitoneally into mast-cell-deficient Mcpt5-Cre(+) × R-DTA mice using littermate mast-cell-sufficient mice as controls. We did not observe any difference between mast-cell-deficient and control mice with regard to weight loss, bacterial clearance, inflammation or cytokine production. We conclude that, despite peritoneal mast cells being activated by S. aureus in vitro, they do not influence the in vivo manifestations of intraperitoneal S. aureus infection.

journal_name

Immunology

journal_title

Immunology

authors

Rönnberg E,Johnzon CF,Calounova G,Garcia Faroldi G,Grujic M,Hartmann K,Roers A,Guss B,Lundequist A,Pejler G

doi

10.1111/imm.12297

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

155-63

issue

2

eissn

0019-2805

issn

1365-2567

journal_volume

143

pub_type

杂志文章

相关文献

IMMUNOLOGY文献大全
  • Mycobacterium tuberculosis conserved hypothetical protein rRv2626c modulates macrophage effector functions.

    abstract：:Secretory proteins of Mycobacterium tuberculosis are the major immunomodulators of the host immune response. Open reading frame (ORF) Rv2626c, encoding a conserved hypothetical protein eliciting a strong humoral immune response in patients with tuberculosis (TB), was shown to be up-regulated upon infection in mice und...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2009.03196.x

    authors： Bashir N,Kounsar F,Mukhopadhyay S,Hasnain SE

    更新日期：2010-05-01 00:00:00

  • Wheat germ agglutinin potentiates uptake of bacteria by murine peritoneal macrophages.

    abstract：:Exposure of thioglycollate-elicited murine peritoneal macrophages to wheat germ agglutinin (WGA) increased markedly the uptake of six different bacteria, which have surface receptors for the lectin. Uptake of Staphylococcus aureus H was higher by 3-5-fold, of S. aureus 52A2 by 1.8-fold, of S. aureus 52A5 by 1.7-fold, ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Gallily R,Vray B,Stain I,Sharon N

    更新日期：1984-08-01 00:00:00

  • The expression and function of Nod-like receptors in neutrophils.

    abstract：:Neutrophils make up an essential part of the innate immune system, and are involved both in the initial responses to pathogens, and in orchestrating later immune responses. Neutrophils recognize pathogens through pattern-recognition receptors (PRRs), which are activated by microbial motifs. The Nod-like receptors (nuc...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2009.03212.x

    authors： Ekman AK,Cardell LO

    更新日期：2010-05-01 00:00:00

  • Increased sensitivity to antigen in high avidity CD8(+) T cells results from augmented membrane proximal T-cell receptor signal transduction.

    abstract：:The functional avidity of a cytotoxic T lymphocyte (CTL) is known to be a critical determinant of the efficacy with which it clears pathogens. High avidity cells, which are by definition highly sensitive to peptide antigen, are superior for elimination of viruses and tumours. Our studies have established the ability o...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2011.03440.x

    authors： Sharma SK,Alexander-Miller MA

    更新日期：2011-07-01 00:00:00

  • PI3K-Akt pathway enhances the differentiation of interleukin-27-induced type 1 regulatory T cells.

    abstract：:Interleukin 27 (IL-27) has been identified as a potent cytokine in the differentiation of type 1 regulatory T (Tr1) cells through interactions with several key elements, including transcription factors such as aryl hydrocarbon receptor and IL-21. Autocrine production of IL-21 is known to be important for maintaining I...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/imm.12789

    authors： Nadya NA,Tezuka H,Ohteki T,Matsuda S,Azuma M,Nagai S

    更新日期：2017-11-01 00:00:00

  • A nitric oxide synthase inhibitor reduces inflammation, down-regulates inflammatory cytokines and enhances interleukin-10 production in carrageenin-induced oedema in mice.

    abstract：:Mice injected in the footpad with carrageenin developed local inflammation which peaked at 48 hr. This delayed-type footpad swelling was significantly reduced in mice injected intraperitoneally (i.p.) with a specific nitric oxide (NO) synthase inhibitor, L-NGmonomethyl-arginine (L-NMMA). The draining lymph node (DLN) ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Ianaro A,O'Donnell CA,Di Rosa M,Liew FY

    更新日期：1994-07-01 00:00:00

  • Titration and circulation of cells mediating delayed type hypersensitivity in normal and cyclophosphamide treated mice during response to sheep red blood cells.

    abstract：:The delayed-type-hypersensitivity (DTH) reaction observed in mice primed i.v. with low doses of sheep red blood cells is not detectable after priming with large doses. Estimation of the number of DTH mediating cells (DTH-C) was performed by titration (D50 determination) using local adoptive transfer of these cells mix...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Marchal G,Milon G,Hurtrel B,Lagrance PH

    更新日期：1978-12-01 00:00:00

  • Phenotypic analysis of peripheral CD4+ CD8+ T cells in the rat.

    abstract：:Among peripheral T cells, the expression of CD4 and CD8 is almost mutually exclusive. However, here we show, using flow cytometric analysis, that ex vivo approximately 6% of rat T cells stained for both CD4 and CD8. These double positive cells were also detected by confocal microscopy. Only around 50% of double positi...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.2000.00071.x

    authors： Kenny E,Mason D,Pombo A,Ramírez F

    更新日期：2000-10-01 00:00:00

  • Analysis of immunoglobulin E VH transcripts in a bronchial biopsy of an asthmatic patient confirms bias towards VH5, and indicates local clonal expansion, somatic mutation and isotype switch events.

    abstract：:Immunoglobulin E (IgE)-dependent mechanisms play a pivotal role in mediating allergic disease. Previously, VH-Cepsilon transcripts from blood or spleen of atopic asthmatics have been analysed for VH gene usage and patterns of somatic mutation. An over-representation of the minor VH5 family has been observed, consisten...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.1999.00910.x

    authors： Snow RE,Djukanovic R,Stevenson FK

    更新日期：1999-12-01 00:00:00

  • The immunoelectron-microscopical demonstration of antibodies against endoplasmic reticulum (microsomes) in chronic aggressive hepatitis and liver cirrhosis.

    abstract：:Electron microscopic findings made on microsomal fractions of rat liver after treatment with microsomal antibodies obtained from the blood of patients diagnosed as having chronic aggressive hepatitis and liver cirrhosis and after incubation with peroxidase-labelled antihuman IgG are described and presented in this pap...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Storch W,Cossel L,Dargel R

    更新日期：1977-06-01 00:00:00

  • Chemerin partly mediates tumor-inhibitory effect of all-trans retinoic acid via CMKLR1-dependent natural killer cell recruitment.

    abstract：:Down-regulated chemerin expression has been reported to correlate with poor prognosis of several types of cancer including melanoma. All-trans retinoic acid (atRA) is a potent inducer of chemerin, and we previously reported that atRA inhibited murine melanoma growth through enhancement of anti-tumor T-cell immunity. H...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/imm.13065

    authors： Song Y,Yin W,Dan Y,Sheng J,Zeng Y,He R

    更新日期：2019-07-01 00:00:00

  • Residues within the alpha subunit sequence 304-322 of muscle acetylcholine receptor forming autoimmune CD4+ epitopes in BALB/c mice.

    abstract：:BALB/c mice develop myasthenic symptoms after immunization with rodent acetylcholine receptor (AChR). After immunization with Torpedo AChR (TAChR), their CD4+ cells become strongly sensitized against a conserved region of the TAChR alpha subunit sequence (residues alpha 304-322), and cross-react vigorously with the ho...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Karachunski PI,Ostlie N,Conti-Tronconi BM,Bellone M

    更新日期：1994-05-01 00:00:00

  • Biosynthesis and secretion of the third component of complement by human endothelial cells in vitro.

    abstract：:The third component of complement (C3) is synthesized and released by cultured human capillary endothelial cells. After the incubation of cells in a methionine-free medium containing 35S-methionine for 48 hr, culture supernatants were immunoprecipitated with anti-human C3 serum. SDS solubilization and 2-ME reduction o...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Ueki A,Sai T,Oka H,Tabata M,Hosokawa K,Mochizuki Y

    更新日期：1987-05-01 00:00:00

  • Suppression of IgE responses by antigen inhalation: failure of tolerance mechanism(s) in newborn rats.

    abstract：:The natural response of immunocompetent adult animals to antigen inhalation is the development of T-lymphocyte mediated tolerance, particularly in the IgE-antibody class (Holt & Sedgwick, 1987). It has been suggested that this process functions as a protective mechanism to limit sensitization to aeroallergens. In the ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Holt PG,Vines J,Britten D

    更新日期：1988-04-01 00:00:00

  • Activation of B lymphocytes by mycoplasma mitogen(s).

    abstract：:Various strains of the murine mycoplasma M. neurolyticum have been shown to induce extensive blast transformation of mouse lymphocytes, comparable in strength to mitogenicity exerted by these mycoplasma species on rat lymphocytes. The data summarized in this report demonstrate that this mitogenic effect is non-specifi...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Naot Y,Ginsburg H

    更新日期：1978-04-01 00:00:00

  • Differential effects of osteopontin on the cytotoxic activity of macrophages from young and old mice.

    abstract：:Osteopontin (OPN) is a secreted phosphoprotein found in body fluids (e.g. plasma, urine, milk) and in mineralized tissues. Its expression is increased in many transformed cells and in normal cells exposed to various cytokines. When stimulated with the inflammatory mediators lipopolysaccharide and interferon-gamma, mou...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.1996.d01-691.x

    authors： Rollo EE,Denhardt DT

    更新日期：1996-08-01 00:00:00

  • Decay-accelerating factor induction by tumour necrosis factor-alpha, through a phosphatidylinositol-3 kinase and protein kinase C-dependent pathway, protects murine vascular endothelial cells against complement deposition.

    abstract：:We have shown that human endothelial cells (EC) are protected against complement-mediated injury by the inducible expression of decay-accelerating factor (DAF). To understand further the importance of DAF regulation, we characterized EC DAF expression on murine EC in vitro and in vivo using a model of glomerulonephrit...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.2003.01733.x

    authors： Ahmad SR,Lidington EA,Ohta R,Okada N,Robson MG,Davies KA,Leitges M,Harris CL,Haskard DO,Mason JC

    更新日期：2003-10-01 00:00:00

  • Time-dependence and selectivity of immunosuppressive agents.

    abstract：:The effects of a variety of agents on the mouse antibody response to sheep red cells (SRC) and Escherichia coli lipopolysaccharide (LPS) were investigated. Agents formed two broad groups according to the time-dependence of their effects. Class I agents inhibited responses if given at any time over a broad period from ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Berenbaum MC

    更新日期：1979-02-01 00:00:00

  • Comparison of cervical and blood T-cell responses to human papillomavirus-16 in women with human papillomavirus-associated cervical intraepithelial neoplasia.

    abstract：:Human papillomaviruses (HPVs) are obligate epithelial pathogens and typically cause localized mucosal infections. We therefore hypothesized that T-cell responses to HPV antigens would be greater at sites of pathology than in the blood. Focusing on HPV-16 because of its association with cervical cancer, the magnitude o...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2567.2006.02465.x

    authors： Passmore JA,Milner M,Denny L,Sampson C,Marais DJ,Allan B,Gumbi PP,Hitzeroth II,Rybicki EP,Williamson AL

    更新日期：2006-12-01 00:00:00

  • Immunological unresponsiveness in mice. II. Cellular basis of immunological unresponsiveness induced in foetal and neonatal mice by transfer of human gamma-globulin by the maternal route.

    abstract：:The cellular basis of the mechanism of immunological tolerance to human gamma-globulin (H gamma G) induced in foetal and neonatal mice by materno-foetal or materno-neonatal transfer after a single injection of tolerogen (deaggregated H gamma G) into the mothers was investigated using a cell transfer system and assays ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Shinka S,Komatsu T,Dohi Y,Amano T

    更新日期：1979-05-01 00:00:00

  • Characterization of a monoclonal antibody against infective larvae of Brugia malayi.

    abstract：:Monoclonal antibodies were produced following immunization of mice with live infective larvae of Brugia malayi. One of these, 46.08.76, is an antibody that promotes adherence of mouse peritoneal macrophages and human peripheral blood leucocytes to the infective larvae of B. malayi and Wuchereria bancrofti, respectivel...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Parab PB,Rajasekariah GR,Chandrashekar R,Alkan SS,Braun DG,Subrahmanyam D

    更新日期：1988-05-01 00:00:00

  • The 5' coding sequence of IL-2 receptor alpha chain mRNA mediates mRNA stabilization by HTLV-1 Rex.

    abstract：:The interleukin-2 receptor alpha chain (IL-2R alpha) is a T-cell growth factor receptor and is known to be induced in helper T cells by infection with human T-cell leukaemia virus type-1 (HTLV-1). The Rex protein of HTLV-1 has been shown to stabilize IL-2R alpha mRNA. Although the 3' untranslated region of many RNA ha...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Kanamori H,Kodama T,Yazaki Y

    更新日期：1995-12-01 00:00:00

  • Mechanisms of clonal abortion tolerogenesis. II. Clonal behaviour of immature B cells following exposure to anti-mu chain antibody.

    abstract：:This paper uses B-lymphocyte cloning methods to quantify the effects of anti-mu chain antibody on immature and mature B cells. Nude mouse spleen lymphocytes were incubated with various concentrations of sheep anti-mouse mu chain antibody for times varying from 10 min to 24 h. They were then washed and plated in the ag...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Nossal GJ,Pike BL,Battye FL

    更新日期：1979-05-01 00:00:00

  • Cyclosporine facilitates B-cell membrane immunoglobulin capping.

    abstract：:The immunomodulatory molecule cyclosporine was found to cause an early acceleration of the capping of mouse B-cell membrane immunoglobulin. This was most marked when the capping process was slightly retarded by keeping the cells at 32 degrees. This is a sign that the drug can cause B-cell membrane alterations. At lowe...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Mosbach-Ozmen L,Humez S,Koponen M,Fonteneau P,Loor F

    更新日期：1986-04-01 00:00:00

  • Langerhans' cell expression of the selectin ligand, sialyl Lewis x.

    abstract：:Cellular adhesion molecules play a central role in leucocyte migration through peripheral blood and tissues. A crucial stage in these events in selectin-mediated adhesion involving E-selectin expressed on activated endothelium interacting with a range of carbohydrate ligands expressed by specific subpopulations of leu...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Ross EL,Barker JN,Allen MH,Chu AC,Groves RW,MacDonald DM

    更新日期：1994-02-01 00:00:00

  • The influence of cyclophosphamide and 6-mercaptopurine on the IgG1 and IgG2 immune response in guinea-pigs.

    abstract：:The purpose of this study was to investigate the effect of cyclophosphamide (CY) and 6-mercaptopurine (6-MP) on the production of the two IgG antibody subclasses in guinea-pigs. Following the intradermal application of dinitrophenylated bovine gamma globulin (DNP20-BGG) emulsified in Freund's incomplete adjuvant (FIA)...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Drössler K,Klima F,Ambrosius H

    更新日期：1981-09-01 00:00:00

  • Soluble FcgammaRIIa inhibits rheumatoid factor binding to immune complexes.

    abstract：:Soluble low-affinity receptors for IgG are known to inhibit immune complex (IC)-mediated inflammation, and expression by leukocytes is elevated in several inflammatory diseases. Immunoglobulin M (IgM) rheumatoid factors (RF), anti-Fc autoantibodies, are found in autoimmune diseases, such as rheumatoid arthritis (RA), ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2567.2003.01652.x

    authors： Wines BD,Gavin A,Powell MS,Steinitz M,Buchanan RR,Mark Hogarth P

    更新日期：2003-06-01 00:00:00

  • Carrier-induced suppression of the antibody response to a 'self' hapten.

    abstract：:Immunization of male rats and monkeys with gonadotropin-releasing hormone (GnRH) conjugated to a carrier results in a dramatic atrophy of the prostate. GnRH, linked to either diphtheria toxoid or tetanus toxoid as carrier, is now being evaluated for its use in the immunotherapy of hormone-dependent prostate enlargemen...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Sad S,Gupta HM,Talwar GP,Raghupathy R

    更新日期：1991-10-01 00:00:00

  • Myeloperoxidase is more efficient than eosinophil peroxidase in the in vitro killing of newborn larvae of Trichinella spiralis.

    abstract：:Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) catalyse the formation of hypochlorite (OCl-) from chloride ions (OCl-) and hydrogen peroxide (H2O2). OCl- proved to be highly toxic for Trichinella spiralis newborn larvae (NBL) in in vitro assays. Using purified human MPO and EPO it was found that even at neutral...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：

    authors： Buys J,Wever R,Ruitenberg EJ

    更新日期：1984-03-01 00:00:00

  • T-cells expressing a chimeric-PD1-Dap10-CD3zeta receptor reduce tumour burden in multiple murine syngeneic models of solid cancer.

    abstract：:Adoptive transfer of T-cells is a promising therapy for many cancers. To enhance tumour recognition by T-cells, chimeric antigen receptors (CARs) consisting of signalling domains fused to receptors that recognize tumour-associated antigens can be expressed in T-cells. While CAR T-cells have shown clinical success for ...

    journal_title：Immunology

    pub_type： 杂志文章

    doi：10.1111/imm.13187

    authors： Parriott G,Deal K,Crean S,Richardson E,Nylen E,Barber A

    更新日期：2020-07-01 00:00:00