Abstract：
:The effects of acetylcholine (ACh) and physostigmine (PHY) on thoracic ganglion neurons of Locusta migratoria were investigated using whole-cell and cell-attached voltage clamp. ACh activated whole-cell currents with variable amplitudes, time course and ion channel block between cells, suggesting differential expression of nicotinic acetylcholine receptor (nAChR) subtypes. This was supported by selective block of the peak of the currents by the alpha7-specific alpha-conotoxin ImI. PHY at 100 microM evoked smaller whole-cell currents with variable amplitudes and marginal desensitization. The PHY/ACh amplitude ratio varied between cells, and was positively related to the time constant of decay of the ACh response. EC50 values for the peak amplitude of the ACh- and PHY-induced currents were 50 microM and 3 microM, respectively. Both agonists activated nAChR, indicated by equal voltage-dependence and reversal potentials and the same pharmacological properties of ACh and PHY responses. In addition, PHY and ACh induced ion channel block. Co-application and cross-desensitization experiments showed that ACh and PHY activate the same nAChR subpopulations. Both agonists activated nicotinic single channels with three conductance levels, which were equal for ACh and PHY, indicating activation of the same nAChR subtypes by both agonists. However, for all levels PHY displayed a lower open probability than ACh. Taken together, different whole-cell responses appear to originate from differential activation, desensitization and ion channel block by ACh and PHY of distinct nAChR populations.
journal_name
Brain Resjournal_title
Brain researchauthors
van den Beukel I,van Kleef RG,Zwart R,Oortgiesen Mdoi
10.1016/s0006-8993(98)00007-9subject
Has Abstractpub_date
1998-04-13 00:00:00pages
263-73issue
2eissn
0006-8993issn
1872-6240pii
S0006-8993(98)00007-9journal_volume
789pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The effect of (S)alpha-fluoromethylhistidine (FMH), a specific inhibitor of histamine synthesis from histidine, on ischemic damage was examined in gerbil brain after forebrain ischemia. Two h after subcutaneous FMH injection, the histamine content of the brain was significantly reduced. Neuronal loss in the CA2 region...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)90784-6
更新日期：1994-12-15 00:00:00
abstract：:Dopamine neurons are overstimulated by drugs of abuse and suffer molecular alterations that lead to addiction behavior. Nurr1 is a transcription factor crucial for dopamine neurons survival and dopamine production, activating the transcription of key genes like tyrosine hydroxylase (TH). Interestingly, nuclear factor-...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.09.031
更新日期：2016-12-01 00:00:00
abstract：:Vanadate (VO-3), an essential trace element with insulin-mimetic actions, produces systemic hyperglycemia following central administration in mice. The hyperglycemic effect is due to specific action of vanadate or its reduced form, vanadyl (VO2+); other ions of similar atomic weight (Cr, Mn) or structure (phosphate) h...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90615-9
更新日期：1987-09-01 00:00:00
abstract：:In the present immunohistochemical study the distribution of nitric oxide synthase (NOS) was studied in various autonomic ganglia and in related peripheral tissues of the rat. For comparison some other neuronal markers including acetylcholinesterase and tyrosine hydroxylase as well as several neuropeptides were analys...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91483-4
更新日期：1994-09-12 00:00:00
abstract：:The ultrastructure of the dorsal column nucleus (DCN) has been investigated at the level of the obex region in normal and experimental toads. Large 'isolated' neurons (greater than 20 micrometer) and clusters of small neurons (less than 20 micrometer) have been identified in this region. Synaptic profiles have been cl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(78)90905-8
更新日期：1978-03-10 00:00:00
abstract：:Based on electrophysiological data showing that repeated cocaine administration produces persistent enhancement of D1 dopamine (DA) receptor-mediated responses in nucleus accumbens (NAc), we investigated whether changes in neurochemical properties of these receptors resulted when rats were injected with cocaine (15 mg...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90783-6
更新日期：1992-02-28 00:00:00
abstract：:Strong evidence obtained from in vivo and ex-vivo studies suggests the existence of interaction between dopaminergic and nitrergic systems. Some of the observations suggest a possible implication of nitric oxide (NO) in dopamine (DA) uptake mechanism. The present work investigated the interaction between both systems ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.12.054
更新日期：2008-03-10 00:00:00
abstract：:Chemotherapy-induced pain is the most common treatment-limiting complication encountered by cancer patients receiving taxane-, vinca alkaloid- or platin-based chemotherapy. Several lines of evidence indicate that activation of pro-inflammatory cascades involving the release of cytokines including tumor necrosis factor...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.07.001
更新日期：2008-09-10 00:00:00
abstract：:Proechimys, a rodent living in the Amazon region, has shown resistance to developing chronic epilepsy when submitted to different experimental models. Recently, many studies have attributed a potent anticonvulsant action to cannabinoid receptor CB1. This study investigated the distribution and expression of the CB1 re...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2010.03.031
更新日期：2010-06-04 00:00:00
abstract：:The present study examined dopamine-immunoreactive neuronal structures using immunohistochemistry in conjunction with an anti-dopamine antiserum, following injection of l-3,4-dihydroxyphenylalanine (L-DOPA) with or without an inhibitor of monoamine oxidase (Pargyline) in the cat brain. L-DOPA injection made it possibl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.05.081
更新日期：2007-09-05 00:00:00
abstract：:The visual system in the pigeon is composed of the tectofugal, thalamofugal and accessory optic pathways. Though their anatomy and physiology have been extensively studied, the functional interactions between these pathways are largely unknown. The present study shows by using multiple electrophysiological techniques ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.11.063
更新日期：2006-01-19 00:00:00
abstract：:Stroke-prone spontaneously hypertensive rats (SHRSP) are a well-characterized, genetic model for stroke. We showed earlier that the structure and function of the tight junctions in SHRSP blood-brain barrier endothelial cells is disturbed prior to stroke. To investigate the molecular events leading to endothelial dysfu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02670-1
更新日期：2001-08-10 00:00:00
abstract：:In the preceding series of articles, the history of vertebrate motoneuron and motor unit neurobiological studies has been discussed. In this article, we select a few examples of recent advances in neuroscience and discuss their application or potential application to the study of motoneurons and the control of movemen...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2011.06.008
更新日期：2011-08-29 00:00:00
abstract：:Changes in the partial pressure of arterial CO2 (PaCO2) regulates cerebrovascular tone and dynamic cerebral autoregulation (CA). Elevations in PaCO2 also increases autonomic neural activity and may alter the arterial baroreflex. We hypothesized that hypercapnia would impair, and hypocapnia would improve, dynamic CA an...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.07.048
更新日期：2008-09-16 00:00:00
abstract：:The effects of 2-day and 7-day cortisol treatment on immunoreactive corticotropin (ACTH) and beta-endorphin concentrations were measured in the cerebral cortex, hippocampus, hypothalamus, and cerebellum in male rats. Plasma ACTH, beta-endorphin, corticosterone, and cortisol levels were also measured in parallel. Corti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90853-6
更新日期：1989-06-12 00:00:00
abstract：:A series of experiments were performed to study the effects of learning upon proteins in the split-brain pigeon. With this preparation, one side of the brain was trained in a color discrimination task while the other side served as a naive control. Proteins from both sides of the brain were separated by one- and two-d...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90349-2
更新日期：1980-09-29 00:00:00
abstract：:Aging is accompanied by a general deterioration of fluid cognitive processes and a reduction in resting cerebral blood flow (CBF). While the two phenomena have been observed independently, it is uncertain whether individual differences in cerebral blood flow are reliably associated with cognitive functioning in older ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2009.12.020
更新日期：2010-02-22 00:00:00
abstract：:Development of noradrenergic responses in the rat dorsal motor nucleus of the vagus (DMV) from postnatal days 0 to 22 was studied by a conventional microelectrode technique using brain slice preparations. Between postnatal days 0 and 6, noradrenaline (NA) caused only alpha 1-adrenoceptor-mediated depolarizations. By p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91170-0
更新日期：1989-07-31 00:00:00
abstract：:In rats under urethane, iontophoretic applications of GABA (30-60 nA) in the str. pyramidale of CA1, showed a rapidly fading inhibitory effect. By contrast, GABA had a well-maintained inhibitory effect in str. radiatum. During iontophoresis of nipecotic acid (30-85 nA) identical applications of GABA in str. pyramidale...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)91309-0
更新日期：1986-02-26 00:00:00
abstract：:The two neurosteroids 3alpha-hydroxy-5alpha-pregnane-20-one (allopregnanolone; AlloP) and pregnenolone sulfate (PregS) affect neuronal GABA(A) receptors differently. While AlloP mainly potentiates the currents through GABA(A) receptors, PregS reduces such currents. The present study aimed at clarifying the interaction...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.11.020
更新日期：2005-02-01 00:00:00
abstract：:This study examined the projection from the rostral nucleus of the solitary tract (rNST) to the medial parabrachial nucleus (mPBN) in male Wistar rats using DiI as a retrograde tracer and biotinylated dextran as an anterograde tracer. Following successful unilateral injection of DiI into the mPBN (n = 8), retrogradely...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00736-6
更新日期：1996-10-21 00:00:00
abstract：:Extracellular recordings were made from 29 respiration-phased neurons in the upper cervical spinal cord (C1-C3) in nine anesthetized rats while ipsilateral and contralateral vagi were stimulated via platinum hook electrodes. Neuronal responses to vagal stimulation were recorded using peristimulus histograms. An accumu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)91143-3
更新日期：1992-10-30 00:00:00
abstract：:Recent studies have demonstrated the neuroprotective properties of the novel imidazoquinoline benzodiazepine receptor partial agonist, PNU-101017, in the gerbil forebrain ischemia model. The compound effectively reduces delayed post-ischemic (5 min bilateral carotid occlusion) hippocampal CA1 neuronal degeneration eve...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00478-8
更新日期：1998-07-06 00:00:00
abstract：:We examined the electrophysiological properties of cholinergic and non-cholinergic neurons in the medial septum-diagonal band complex (MSDB) of the rat in the in vitro slice preparation. Cells were identified electrophysiologically, filled with Lucifer yellow, fixed and processed for immunohistochemistry with fluoresc...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91106-q
更新日期：1990-04-09 00:00:00
abstract：:An interaction between angiotensin II (Ang II) receptors and alpha 2-adrenoceptors was evaluated in the nucleus tractus solitarii (NTS) of the normotensive Wistar-Kyoto rat (WKY) and of the spontaneously hypertensive rat (SHR) using quantitative receptor autoradiography and cardiovascular analysis. In the WKY rat, Ang...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00220-k
更新日期：1995-05-08 00:00:00
abstract：:Extracellular signal-regulated kinase 1/2 (ERK1/2) plays diverse roles in the central nervous system. Activation of ERK1/2 has been observed in various types of neuronal excitation, including seizure activity in vivo and in vitro. However, studies examining ERK1/2 activity and its substrate phosphorylation in parallel...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.08.023
更新日期：2015-11-02 00:00:00
abstract：:Lateral ventricular injection of antibodies to dynorphin A(1-13) was previously shown to elevate lateral hypothalamic stimulation frequency threshold for eliciting feeding behavior. The antibodies utilized in that study cross-react completely with dynorphin A(1-17) and, to a lesser extent, dynorphin A(1-8). In the pre...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90284-i
更新日期：1990-01-22 00:00:00
abstract：:Samples of 14 brains of autopsied patients suffering from dementia of the Alzheimer type were examined. In Congo red-hemalum stained 14-microns-thick sections of the hippocampus 15 tangle-bearing and 25-30 unaffected nerve cells of area CA1 per case were measured for cell size and nucleolar size utilizing an interacti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90843-3
更新日期：1989-05-22 00:00:00
abstract：:Aging represents a major risk factor for numerous illnesses that are of increasing importance to society, including two of the most prevalent: diabetes and Alzheimer's disease. Studies have shown that diabetes is a risk factor for spontaneous Alzheimer's disease. While these studies suggest that diabetes can contribut...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2015.04.026
更新日期：2015-07-30 00:00:00
abstract：:Wheat germ agglutinin conjugated to horseradish peroxidase (WGA-HRP) was used to study transneuronal transport in the developing rat visual pathways. Intraocular injections of WGA-HRP were made in neonatal albino rat pups at different ages from the day of birth, postnatal day 0 (P0), to one month of age. Transneuronal...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(88)90087-9
更新日期：1988-01-01 00:00:00
