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Characterization of the ovalbumin-specific TCR transgenic line OT-I: MHC elements for positive and negative selection.

Abstract：

:The present report provides the first extensive characterization of the OT-I TCR transgenic line, which produces MHC class I-restricted, ovalbumin-specific, CD8+ T cells (OT-I cells). These cells are shown to be positively selected in vivo in H-2b C57BL/6 mice and in bm5 mice, which express the Kbm5 mutant molecule. In contrast, OT-I cells were not selected by mutant Kb molecules in bm1, bm3, bm8, bm10, bm11 or bm23 mice. Interestingly, however, when positive selection was examined in vitro in foetal thymic organ culture (FTOC), bm1 and bm8 were still poorly selective, but the bm3 haplotype now selected as efficiently as B6. The ability to select in vitro correlated with the capacity to present the ovalbumin (OVA) peptide to OT-I cells, as measured by induction of an OVA-specific proliferative response. These results suggest that a lower affinity TCR:MHC interaction may be necessary for positive selection in FTOC compared with selection in situ.

journal_name

Immunol Cell Biol

journal_title

Immunology and cell biology

authors

Clarke SR,Barnden M,Kurts C,Carbone FR,Miller JF,Heath WR

doi

10.1046/j.1440-1711.2000.00889.x

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

110-7

issue

2

eissn

0818-9641

issn

1440-1711

pii

icb889

journal_volume

78

pub_type

杂志文章

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