Abstract:
:Vaccines against group B meningococcal infection tested in several field trials have all been extracts of the outer membrane of the bacteria. We have developed a single component vaccine based on the class 1 outer membrane protein P1 produced in a heterologous host Bacillus subtilis, and describe here its immunizing properties. The purified and denatured protein BacP1 was solubilized in SDS, followed by addition of an excess of a second detergent (Zwittergent 3-14 or Triton X-100). Immunization of mice showed that this process led to at least partial reconstitution of the native epitopes of the P1 protein. The immunogenicity of these BacP1 detergent preparations was further improved when administered together with adjuvants (aluminium hydroxide or monophosphoryl lipid A); high titers of antibodies were thus obtained with vaccine doses as low as 2 micrograms of protein. The antibodies elicited were essentially of IgG and reactive with protective epitopes present on the surface of meningococci. The bactericidal activity of the sera showed a good correlation to antibodies of the IgG1 and IgG2 isotypes, concomitantly increased in most sera.
journal_name
Vaccinejournal_title
Vaccineauthors
Idänpään-Heikkilä I,Wahlström E,Muttilainen S,Nurminen M,Käyhty H,Sarvas M,Mäkelä PHdoi
10.1016/0264-410x(95)00263-zsubject
Has Abstractpub_date
1996-06-01 00:00:00pages
886-91issue
9eissn
0264-410Xissn
1873-2518pii
0264410X9500263Zjournal_volume
14pub_type
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