Abstract：
:EG95 is a recombinant vaccine protein that elicits protection against hydatid disease in sheep. Previous studies have shown that the host-protective epitopes on EG95 depend on correct conformation and cannot be represented by simple "linear" peptides. By screening random peptide phage display libraries with polyclonal antibodies directed against conformation-dependant epitopes of EG95, we have selected a number of peptides that mimic these epitopes. The selected peptides did not show sequence homology to EG95. Antigen binding assays involving these peptides have provided evidence of at least four conformationally-dependant epitope regions on EG95. One of the selected peptides, E100, has been used to purify antibodies from anti-sera raised in sheep vaccinated with EG95. This yielded monospecific antibodies capable of recognizing recombinant EG95 in ELISA and native EG95 in Western blot assays. This antibody was demonstrated to be effective in antibody-dependant complement-mediated in vitro killing of Echinococcus granulosus oncospheres. Peptide E100 may represent the basis for a quality control assay for EG95 production, and has the potential to become a component of a synthetic peptide-based vaccine against E. granulosus.
journal_name
Vaccinejournal_title
Vaccineauthors
Read AJ,Casey JL,Coley AM,Foley M,Gauci CG,Jackson DC,Lightowlers MWdoi
10.1016/j.vaccine.2008.11.096subject
Has Abstractpub_date
2009-02-11 00:00:00pages
1024-31issue
7eissn
0264-410Xissn
1873-2518pii
S0264-410X(08)01681-2journal_volume
27pub_type
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