当前位置： SCI文献检索 > VACCINE期刊下所有文献 > Carrier-dependent specificity of antibodies to a conserved peptide determinant of gp120.

Carrier-dependent specificity of antibodies to a conserved peptide determinant of gp120.

Abstract：

:Amino acid residues 421-436 constitute a comparatively conserved determinant of gp120 that participates in the binding of host cell CD4 receptors by HIV-1. We compared the immunogenicity of synthetic Cys-gp120 (421-436) conjugated to KLH via the N terminal Cys residue (KLH-I) and gp120 (421-436) extended at its N terminus with a 15 residue tetanus toxoid T cell epitope (T-I) in non-autoimmune mice (BALB/cstrain) and Fas-defective autoimmune mice (MRL/lpr strain). Both immunogens elicited high titer Abs detected as the binding to gp120 (421-436) conjugated to bovine serum albumin (BSA-I) immobilized in ELISA plates. Abs from KLH-I immunized mice displayed binding to full-length gp120 but the Abs from T-I immunized mice did not. Proteins unrelated in sequence to gp120 did not bind the Abs. Soluble I and T-I failed to compete with immobilized BSA-I for binding to anti-KLH-I Abs, whereas these peptides inhibited anti-T-I Ab binding by BSA-I (rank potency order: BSA-I > T-I > I). These results indicate the influence of the carrier protein on the specificity of Abs to synthetic I. Low level BSA-I and gp120 binding Abs were detected in sera from non-immunized MRL/lpr mice. Similar Ab binding titers and specificity profiles were evident in MRL/lpr and BALB/c mice following immunization with KLH-I and T-I, indicating that pre-existing immunity to gp120 in the former strain does not influence the magnitude or specificity of the Ab response.

journal_name

Vaccine

journal_title

Vaccine

authors

Karle S,Nishiyama Y,Taguchi H,Zhou YX,Luo J,Planque S,Hanson C,Paul S

doi

10.1016/s0264-410x(02)00504-2

subject

Has Abstract

pub_date

2003-03-07 00:00:00

pages

1213-8

issue

11-12

eissn

0264-410X

issn

1873-2518

pii

S0264410X02005042

journal_volume

21

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Analysis of adenovirus-induced immunity to infection with Listeria monocytogenes: Fading protection coincides with declining CD8 T cell numbers and phenotypic changes.

    abstract：:Defining correlates of T cell mediated protection is important in order to accelerate the development of efficient T cell based vaccines conferring long-term immunity. Extensive studies have provided important insight regarding the characteristics and functional properties of the effector and memory CD8 T cells induce...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.03.080

    authors： Jahn ML,Steffensen MA,Christensen JP,Thomsen AR

    更新日期：2018-05-11 00:00:00

  • Effect of emergency FMD vaccine antigen payload on protection, sub-clinical infection and persistence following direct contact challenge of cattle.

    abstract：:Previous work, in sheep vaccinated with emergency foot-and-mouth disease (FMD) vaccine, indicated the benefit of increasing the antigen payload in inhibiting local virus replication and consequently persistence following an indirect aerosol challenge with a virus homologous to the vaccine strain. The work presented he...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.01.037

    authors： Cox SJ,Voyce C,Parida S,Reid SM,Hamblin PA,Hutchings G,Paton DJ,Barnett PV

    更新日期：2006-04-12 00:00:00

  • HSP65 DNA as therapeutic strategy to treat experimental paracoccidioidomycosis.

    abstract：:The conventional treatment for paracoccidioidomycosis, the most prevalent mycosis in Latin America, involves long periods of therapy resulting in sequels and high frequency of relapses. The search for new alternatives of treatment is necessary. Previously, we have demonstrated that the hsp65 gene from Mycobacterium le...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.11.062

    authors： Ribeiro AM,Bocca AL,Amaral AC,Souza AC,Faccioli LH,Coelho-Castelo AA,Figueiredo F,Silva CL,Felipe MS

    更新日期：2010-02-10 00:00:00

  • The tuberculosis vaccine H4:IC31 is safe and induces a persistent polyfunctional CD4 T cell response in South African adults: A randomized controlled trial.

    abstract：BACKGROUND:New, more effective vaccines to prevent tuberculosis (TB) disease are needed urgently. H4:IC31 is an investigational vaccine that contains a fusion protein of the immunodominant antigens TB10.4 and Ag85B, formulated in IC31 adjuvant. We assessed the safety and immunogenicity of H4:IC31 in South African adult...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2015.05.036

    authors： Geldenhuys H,Mearns H,Miles DJ,Tameris M,Hokey D,Shi Z,Bennett S,Andersen P,Kromann I,Hoff ST,Hanekom WA,Mahomed H,Hatherill M,Scriba TJ,H4:IC31 Trial Study Group.,van Rooyen M,Bruce McClain J,Ryall R,de Bruyn G,H4:

    更新日期：2015-07-09 00:00:00

  • Anti-tumor effect of DNA-based vaccination and dSLIM immunomodulatory molecules in mice with Ph+ acute lymphoblastic leukaemia.

    abstract：:Since the prognosis of patients with Philadelphia chromosome positive acute lymphoblastic leukaemia (Ph+ ALL) still remains poor, new relapse prevention strategies are needed. We evaluated the pre-immunization of mice with DNA-based vaccines subsequently challenged by the syngeneic Ph+ ALL cell line BM185. Ballistic t...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.06.094

    authors： Köchling J,Prada J,Bahrami M,Stripecke R,Seeger K,Henze G,Wittig B,Schmidt M

    更新日期：2008-08-26 00:00:00

  • Seroprevalence of anti-polio antibodies in a population 7 months to 39 years of age in Uruguay: Implications for future polio vaccination strategies.

    abstract：:This study evaluated the seroprevalence of poliovirus types 1, 2 and 3 antibodies and vaccination coverage in 780 subjects aged between 7 months and 39 years in Montevideo, Uruguay, where oral polio vaccine (OPV) is used. Antibody titers were measured and seroprotection rates and geometric mean titers (GMTs) were comp...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.02.042

    authors： Pírez MC,Olivera I,Diabarboure H,Montano A,Barañano R,Badía F,Bonnet MC

    更新日期：2009-05-05 00:00:00

  • Mucosal immunogenicity of genetically detoxified derivatives of heat labile toxin from Escherichia coli.

    abstract：:Using a fixed dose of antigen, the immune response to detoxified mutants of LT-WT following intranasal (i.n.), subcutaneous (s.c.) and oral (i.g.) immunisation has been studied. When given i.n., both LT-WT and mutant toxin, K63, generated significant levels of toxin-specific IgG in the serum, and the levels of IgA in ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)80100-x

    authors： Douce G,Giuliani MM,Giannelli V,Pizza MG,Rappuoli R,Dougan G

    更新日期：1998-07-01 00:00:00

  • The efficacy of recombinant turkey herpesvirus vaccines targeting the H5 of highly pathogenic avian influenza virus from the 2014-2015 North American outbreak.

    abstract：:The outbreak of highly pathogenic avian influenza virus in North American poultry during 2014 and 2015 demonstrated the devastating effects of the disease and highlighted the need for effective emergency vaccine prevention and control strategies targeted at currently circulating strains. This study evaluated the effic...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.11.026

    authors： Balzli CL,Bertran K,Lee DH,Killmaster L,Pritchard N,Linz P,Mebatsion T,Swayne DE

    更新日期：2018-01-02 00:00:00

  • Capacity for a global vaccine safety system: the perspective of national regulatory authorities.

    abstract：:Confidence in vaccine safety is critical to national immunization strategies and to global public health. To meet the Millenium Development Goals, and buoyed by the success of new vaccines produced in developing countries, the World Health Organization has been developing a strategy to establish a global system for ef...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.05.045

    authors： Graham JE,Borda-Rodriguez A,Huzair F,Zinck E

    更新日期：2012-07-13 00:00:00

  • Molecular characterisation of pneumococcal serotype 16F: Established predominant carriage and otitis media serotype in the 7vPCV era.

    abstract：:Young Australian Aboriginal children in remote communities experience very high rates of pneumococcal carriage and otitis media. Prior to introduction of the 7-valent pneumococcal conjugate vaccine (7vPCV, Prevenar), serotype 16F was an important type found in nasal and ear discharge swabs. Since commencement of pneum...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.09.016

    authors： Marsh RL,Smith-Vaughan H,Beissbarth J,Hare K,Kennedy M,Wigger C,Mellon G,Stubbs E,Gadil JR,Pettit A,Mackenzie G,Tipakalippa P,Morris PS,Leach AJ

    更新日期：2007-03-22 00:00:00

  • The use of transgenic Plasmodium berghei expressing the Plasmodium vivax antigen P25 to determine the transmission-blocking activity of sera from malaria vaccine trials.

    abstract：:P25 is a major surface protein of Plasmodium ookinetes. Antibodies against P25 prevent the formation of oocysts in the mosquito and thereby block transmission of the parasite through an endemic population. Plasmodium vivax transmission-blocking vaccines based on Pv25 have undergone human trials and inhibit transmissio...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.09.035

    authors： Ramjanee S,Robertson JS,Franke-Fayard B,Sinha R,Waters AP,Janse CJ,Wu Y,Blagborough AM,Saul A,Sinden RE

    更新日期：2007-01-15 00:00:00

  • Knowledge, attitudes and beliefs related to seasonal influenza vaccine among pregnant women in Thailand.

    abstract：BACKGROUND:In 2009, Thailand recommended pregnant women be prioritized for influenza vaccination. Vaccine uptake among Thai pregnant women is lower than other high-risk groups. METHODS:During December 2012-April 2013, we conducted a cross-sectional survey of a convenience sample of Thai pregnant women aged ≥ 15 years ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2016.01.056

    authors： Ditsungnoen D,Greenbaum A,Praphasiri P,Dawood FS,Thompson MG,Yoocharoen P,Lindblade KA,Olsen SJ,Muangchana C

    更新日期：2016-04-19 00:00:00

  • Induction of autoantibody production but not autoimmune disease in HEL transgenic mice vaccinated with HEL in combination with CpG or control oligodeoxynucleotides.

    abstract：:CpG oligodeoxynucleotides (ODN) are synthetic DNA sequences that mimic bacterial DNA, and bind to the TLR9 receptor. The cells that express TLR9, B cells and dendritic cells, are stimulated by CpG ODN and induce innate and acquired immune responses. Because CpG ODN induce antigen-independent immune activation there ha...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2003.11.055

    authors： Wang Y,Krieg AM

    更新日期：2004-06-30 00:00:00

  • The purification and protective capacity of Bordetella pertussis outer membrane proteins.

    abstract：:The whole cell vaccine (WCV) of Bordetella pertussis is protective in the intracerebral (i.c.) mouse protection assay. We found a correlation between the i.c. mouse protection assay potency and the presence of the virulence-associated outer membrane proteins (OMPs) in outer membrane complexes (OMC). The virulence-asso...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(94)00040-t

    authors： Hamstra HJ,Kuipers B,Schijf-Evers D,Loggen HG,Poolman JT

    更新日期：1995-01-01 00:00:00

  • Development of an oral vaccine for immunisation of rainbow trout (Oncorhynchus mykiss) against viral haemorrhagic septicaemia.

    abstract：:In the European Union Viral Haemorrhagic Septicaemia (VHS) eradication is still based on stamping out. Due to the lack of effective low cost vaccines immune prophylaxis is currently not used to combat VHS. This paper describes a new oral delivery method for immunisation of trout with attenuated virus. The vaccine cons...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.11.065

    authors： Adelmann M,Köllner B,Bergmann SM,Fischer U,Lange B,Weitschies W,Enzmann PJ,Fichtner D

    更新日期：2008-02-06 00:00:00

  • Expression of Cathepsin S in BCG converts it into a pro-apoptotic and highly immunogenic strain.

    abstract：BACKGROUND:BCG vaccine, introduced almost 100years ago, is the only option to prevent TB disease. It effectively protects newborns from meningeal TB but fails to prevent adult pulmonary TB. TB kills 1.3million people annually in areas where BCG vaccination is widely practiced. Thus, more effective TB vaccines are urgen...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.02.065

    authors： Lau A,Singh V,Soualhine H,Hmama Z

    更新日期：2017-04-11 00:00:00

  • Structural analysis and immunogenicity of recombinant major envelope protein (rA27L) of buffalopox virus, a zoonotic Indian vaccinia-like virus.

    abstract：:Buffalopox virus (BPXV), an Indian variant of vaccinia virus (VACV), is a zoonotic agent and affects buffaloes, cattle and humans. A27L is one of the conserved major immuno-dominant envelope proteins of orthopox viruses (OPVs) involved in viral entry/maturation and elicits neutralizing antibodies. In this study, the A...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.08.058

    authors： Kumar A,Yogisharadhya R,Bhanuprakash V,Venkatesan G,Shivachandra SB

    更新日期：2015-10-05 00:00:00

  • Comparison of pertussis surveillance systems in Europe.

    abstract：:We compared pertussis surveillance systems of 16 European countries in the period 1998-2002. In twelve out of sixteen countries the system covered the general population. Ten countries relied on WHO case definition for surveillance of pertussis. Eleven countries applied laboratory tests, and eight of them used PCR for...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.07.023

    authors： Tozzi AE,Pandolfi E,Celentano LP,Massari M,Salmaso S,Ciofi degli Atti ML,EUVAC-NET Study Group.

    更新日期：2007-01-04 00:00:00

  • Secondary in vitro B lymphocyte (antibody) response to microbial antigens: use in appraisal of vaccine immunogenicity and cytokine immunoregulation.

    abstract：:In order to perform preliminary evaluations of subunit vaccine candidates before extensive trials in large food-producing animals, an in vitro B-lymphocyte response assay, based on the principles of an ELISA, was established. The assay was developed in detail for the porcine system using antigens from the Gram-negativ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(91)90149-z

    authors： Ohmann HB,McDougall L,Potter A

    更新日期：1991-03-01 00:00:00

  • Fish soluble Toll-like receptor (TLR)5 amplifies human TLR5 response via physical binding to flagellin.

    abstract：:Fish has a soluble form of TLR5 ortholog (TLR5S), which does not exist in mammals. We identified TLR5S from rainbow trout and named rtTLR5S, which was about 38% homologous to the extracellular domains of human (hu) and mouse TLR5. Adjuvancy of rtTLR5S to flagellin response by human TLR5 (huTLR5) was tested in this stu...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.11.003

    authors： Tsujita T,Ishii A,Tsukada H,Matsumoto M,Che FS,Seya T

    更新日期：2006-03-15 00:00:00

  • Assessment of potential miRNA biomarkers of VERO-cell tumorigenicity in a new line (AGMK1-9T7) of African green monkey kidney cells.

    abstract：:Patterns of microRNA expression appear to delineate the process of spontaneous neoplastic development-transformation (SPNDT) occurring in the African green monkey kidney (AGMK) VERO cell line (Teferedegne et al., 2010). Analysis of microarray data identified 6 microRNAs whose high-level of expression peaked when the W...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.04.004

    authors： Teferedegne B,Rotroff DM,Macauley J,Foseh G,Lewis G,Motsinger-Rief A,Lewis AM Jr

    更新日期：2017-10-04 00:00:00

  • Low doses of flagellin-L2 multimer vaccines protect against challenge with diverse papillomavirus genotypes.

    abstract：:Genetically modified bacterial flagellin (Fla), a Toll-like receptor-5 (TLR5) ligand, was evaluated as a fusion partner for human papillomavirus (HPV) L2-based immunogens in two animal challenge models; either cutaneous inoculation of rabbits with HPV 'quasivirions' containing cottontail rabbit papillomavirus (CRPV) g...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2014.04.032

    authors： Kalnin K,Tibbitts T,Yan Y,Stegalkina S,Shen L,Costa V,Sabharwal R,Anderson SF,Day PM,Christensen N,Schiller JT,Jagu S,Roden RB,Almond J,Kleanthous H

    更新日期：2014-06-12 00:00:00

  • Comparison of DNA application methods to reduce BRSV shedding in cattle.

    abstract：:We compared the protection afforded by three different DNA application methods against bovine respiratory syncytial virus (BRSV) infection in cattle. A synthetic gene that codes for the G protein of BRSV was inserted into a eukaryotic vector and was used in the vaccine. Intradermal (i.d.) application with a needleless...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(97)00198-9

    authors： Schrijver RS,Langedijk JP,Keil GM,Middel WG,Maris-Veldhuis M,Van Oirschot JT,Rijsewijk FA

    更新日期：1998-01-01 00:00:00

  • Immunogenicity of influenza vaccines administered to pregnant women in randomized clinical trials in Mali and South Africa.

    abstract：BACKGROUND:A key consideration for expanding recommendations for influenza vaccination is a robust assessment of immunogenicity and efficiency of transplacental antibody transfer after maternal vaccination. METHODS:We pooled data from two trials of maternal influenza vaccination to analyze vaccine immunogenicity with ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2020.07.020

    authors： Amin AB,Nunes MC,Tapia MD,Madhi SA,Cutland CL,Wairagkar N,Omer SB,for BMGF Supported Maternal Influenza Immunization Trials Investigators Group.

    更新日期：2020-09-22 00:00:00

  • Vaccination with recombinant tick antigens for the control of Ixodes scapularis adult infestations.

    abstract：:Antigens protective against Ixodes scapularis infestations were identified by cDNA expression library immunization (ELI) and analysis of expressed sequenced tags (EST). Three cDNAs protective against larval tick infestations, 4F8, with homology to a nucleotidase, and 4D8 and 4E6 of unknown function, were characterized...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.08.004

    authors： Almazán C,Kocan KM,Blouin EF,de la Fuente J

    更新日期：2005-11-16 00:00:00

  • Characterization of the protective immune response to Yersinia pseudotuberculosis infection in mice vaccinated with an LcrV-secreting strain of Lactococcus lactis.

    abstract：BACKGROUND:Pseudotuberculosis is an infection caused by the bacterial enteropathogen Yersinia pseudotuberculosis and is considered to be a significant problem in veterinary medicine. We previously found that intranasal administration of a recombinant Lactococcus lactis strain that secretes the low-calcium response V (L...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2016.09.060

    authors： Daniel C,Titecat M,Poiret S,Cayet D,Boutillier D,Simonet M,Sirard JC,Lemaître N,Sebbane F

    更新日期：2016-11-11 00:00:00

  • Targeted expression of HTLV-I envelope proteins in muscle by DNA immunization of mice.

    abstract：:We have compared two types of plasmids for DNA immunization against HTLV-I envelope glycoproteins. One type of plasmid contains the coding DNA of the complete envelope gene of HTLV-I under the control of the CMV promoter with (CMVenvLTR) or without (CMVenv) the tax/rex genes. The second type contains the coding DNA of...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(99)00565-4

    authors： Armand MA,Grange MP,Paulin D,Desgranges C

    更新日期：2000-04-28 00:00:00

  • Group B streptococcal conjugate vaccines elicit functional antibodies independent of strain O-acetylation.

    abstract：:Recently, it was discovered that sialic acid residues on group B streptococcal (GBS) capsular polysaccharides (CPS) are O-acetylated. Since GBS vaccine development has focused on de-O-acetylated CPS, it became germane to investigate the influence of de-O-acetylated GBS vaccine formulations on functional activity of se...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.05.039

    authors： Pannaraj PS,Edwards MS,Ewing KT,Lewis AL,Rench MA,Baker CJ

    更新日期：2009-07-16 00:00:00

  • Role of different genes in the CS6 operon for surface expression of Enterotoxigenic Escherichia coli colonization factor CS6.

    abstract：:Coli surface antigen 6, CS6, is one of the most prevalent colonization factors (CFs) associated with Enterotoxigenic Escherichia coli (ETEC) bacteria, the most common cause of diarrhea among infants and children in developing countries. The CS6 operon encodes two structural subunit proteins, CssA and CssB, a chaperon,...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.07.091

    authors： Tobias J,Lebens M,Källgård S,Nicklasson M,Svennerholm AM

    更新日期：2008-10-03 00:00:00

  • The role of IL-10 and IgG1 in the protection and granulomatous response in Schistosoma mansoni P24-immunized mice.

    abstract：:Previous work by our laboratory identified a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, designated PIII, able to elicit significant in vitro cell proliferation, and lower in vitro and in vivo granuloma formation. In the present work, we investigated some biological activities of P24, an ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(00)00265-6

    authors： Zouain CS,Gustavson S,Oliveira SC,Azevedo V,Alves JB,Goes AM

    更新日期：2000-12-08 00:00:00