Secondary in vitro B lymphocyte (antibody) response to microbial antigens: use in appraisal of vaccine immunogenicity and cytokine immunoregulation.


:In order to perform preliminary evaluations of subunit vaccine candidates before extensive trials in large food-producing animals, an in vitro B-lymphocyte response assay, based on the principles of an ELISA, was established. The assay was developed in detail for the porcine system using antigens from the Gram-negative bacterium Actinobacillus pleuropneumoniae, but is shown to be applicable to other species and antigens, including viral components. It is further shown that B-cell activity in the assay is dependent on T-helper cells as well as macrophages and/or their secretory products. Thus, in addition to providing a tool for evaluation of T and B memory cell activity, the system also lends itself to dissection of T-B cell collaboration and the regulatory functions of interleukins in secondary (in vitro) antibody responses.






Ohmann HB,McDougall L,Potter A




Has Abstract


1991-03-01 00:00:00
















  • Hepatitis A and B in children and adolescents--what can we learn from Puglia (Italy) and Catalonia (Spain)?

    abstract::Viral hepatitis remains a major contributor to the global disease burden. Mass immunisation strategies against hepatitis B have been adopted by more than 90 developing and industrialised countries. Countries with low hepatitis A endemicity are experiencing cyclical outbreaks and an epidemiological shift, with larger n...


    pub_type: 杂志文章


    authors: Lopalco PL,Salleras L,Barbuti S,Germinario C,Bruguera M,Buti M,Domínguez A

    更新日期:2000-10-15 00:00:00

  • Platform technology to deliver prophylactic molecules orally: an example using the Class A select agent Yersinia pestis.

    abstract::Consumed for centuries, lactic acid bacteria are excellent candidates for the development of safe mucosal delivery vehicles for prophylactic and therapeutic molecules. We have recently reported that the immune response to an effective OspA-expressing L. plantarum vaccine for Lyme disease is modulated by the lipid modi...


    pub_type: 杂志文章


    authors: del Rio B,Fuente JL,Neves V,Dattwyler R,Seegers JF,Gomes-Solecki M

    更新日期:2010-09-24 00:00:00

  • Immunopathological responses and kinetics of Brucella melitensis Rev 1 infection after subcutaneous or conjunctival vaccination in rams.

    abstract::The innocuousness of the Brucella melitensis Rev 1 live attenuated vaccine strain has never been fully assessed in rams. The immunopathological responses and the kinetics and distribution of the infection induced by this strain were determined after subcutaneous or conjunctival vaccination in both young (3-4 months ol...


    pub_type: 杂志文章


    authors: Muñoz PM,de Miguel MJ,Grilló MJ,Marín CM,Barberán M,Blasco JM

    更新日期:2008-05-19 00:00:00

  • Immunization with PfEMP1-DBL1alpha generates antibodies that disrupt rosettes and protect against the sequestration of Plasmodium falciparum-infected erythrocytes.

    abstract::A family of parasite antigens known as Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is believed to play an important role in the binding of infected erythrocytes to host receptors in the micro-vasculature. Available data advocates the existence of a subset of very adhesive (rosetting, auto-agglutinati...


    pub_type: 杂志文章


    authors: Chen Q,Pettersson F,Vogt AM,Schmidt B,Ahuja S,Liljeström P,Wahlgren M

    更新日期:2004-07-29 00:00:00

  • Human airway epithelial cells present antigen to influenza virus-specific CD8+ CTL inefficiently after incubation with viral protein together with ISCOMATRIX.

    abstract::In the present paper, an in vitro model was established in which the interaction between influenza virus-specific CD8+ T cells and human airway epithelial cells can be studied. To this end, the human lung epithelial cell line A549 was transduced with the HLA-A*0201 gene. This MHC class I allele is involved in the pres...


    pub_type: 杂志文章


    authors: Rimmelzwaan GF,Boon AC,Geelhoed-Mieras MM,Voeten JT,Fouchier RA,Osterhaus AD

    更新日期:2004-07-29 00:00:00

  • Development and phase 1 clinical testing of a conjugate vaccine against meningococcus A and C.

    abstract::A conjugate vaccine against meningococcus A and C was prepared using the non-toxic mutant of diphtheria toxin CRM 197 as a carrier protein. Capsular polysaccharides of Neisseria meningitidis group A and C were hydrolysed and the resulting oligosaccharides were then coupled to CRM 197 in order to obtain conjugates with...


    pub_type: 临床试验,杂志文章


    authors: Costantino P,Viti S,Podda A,Velmonte MA,Nencioni L,Rappuoli R

    更新日期:1992-01-01 00:00:00

  • Antigenic differences among Newcastle disease virus strains of different genotypes used in vaccine formulation affect viral shedding after a virulent challenge.

    abstract::Strains of Newcastle disease virus (NDV) can be separated into genotypes based on genome differences even though they are antigenically considered to be of a single serotype. It is widely recognized that an efficacious Newcastle disease (ND) vaccine made with any NDV does induce protection against morbidity and mortal...


    pub_type: 杂志文章


    authors: Miller PJ,King DJ,Afonso CL,Suarez DL

    更新日期:2007-10-10 00:00:00

  • Socioeconomic factors play a more important role in childhood vaccination coverage than parental perceptions: a cross-sectional study in Greece.

    abstract::To identify predictive factors of complete and age-appropriate vaccination status in Greece, we conducted a cross-sectional study, using stratified cluster sampling, among children attending the first year of the Greek Grammar school (about 6 years of age) and their parents/guardians. Almost 88% (N=3878) of pupils in ...


    pub_type: 杂志文章


    authors: Danis K,Georgakopoulou T,Stavrou T,Laggas D,Panagiotopoulos T

    更新日期:2010-02-17 00:00:00

  • Immunology of scorpion toxins and perspectives for generation of anti-venom vaccines.

    abstract::Scorpions and other venomous animals contain concentrates of biologically active substances developed to block vital physiological and biochemical functions of the victims. These have contrasting human health concerns, provide important pharmacological raw material and pose a serious threat to human life and health in...


    pub_type: 杂志文章,评审


    authors: Gazarian KG,Gazarian T,Hernández R,Possani LD

    更新日期:2005-05-16 00:00:00

  • A strategy of antigen incorporation into exosomes: comparing cross-presentation levels of antigens delivered by engineered exosomes and by lentiviral virus-like particles.

    abstract::Among strategies aimed at developing new nanoparticle-based vaccines, exosomes hold much promise. They are nanovesicles released by basically all eukaryotic cell types originating from intraluminal vesicles which accumulate in multivesicular bodies. Exosomes have immunogenic properties whose strength correlates with t...


    pub_type: 杂志文章


    authors: Lattanzi L,Federico M

    更新日期:2012-11-26 00:00:00

  • Hepatitis A vaccination coverage among adolescents (13-17 years) in the United States, 2008-2016.

    abstract:BACKGROUND:The hepatitis A (HepA) vaccine was recommended by the Advisory Committee on Immunization Practices (ACIP) incrementally from 1996 to 1999. In 2006, HepA vaccine was recommended (1) universally for children aged 12-23 months, (2) for persons who are at increased risk for infection, or (3) for any person wishi...


    pub_type: 历史文章,杂志文章


    authors: Nelson NP,Yankey D,Singleton JA,Elam-Evans LD

    更新日期:2018-03-14 00:00:00

  • Combined vaccination against IL-5 and eotaxin blocks eosinophilia in mice.

    abstract::Interleukin-5 (IL-5) is a cytokine which is essential for the maturation of eosinophils in bone marrow and for their release into the blood. Eotaxin is a CC type chemokine implicated in the recruitment of eosinophils in a variety of inflammatory disorders. Since eosinophil-activity is governed by these two pathways, w...


    pub_type: 杂志文章


    authors: Zou Y,Sonderegger I,Lipowsky G,Jennings GT,Schmitz N,Landi M,Kopf M,Bachmann MF

    更新日期:2010-04-19 00:00:00

  • Development of an in vitro-based potency assay for anthrax vaccine.

    abstract::The potency assay currently used to evaluate consistency of manufacture for the anthrax vaccine is contingent upon meeting specified parameters after statistical analysis of the percent survival and time to death of vaccinated guinea pigs after challenge with spores of a virulent strain of Bacillus anthracis. During t...


    pub_type: 杂志文章


    authors: Little SF,Webster WM,Ivins BE,Fellows PF,Norris SL,Andrews GP

    更新日期:2004-07-29 00:00:00

  • Influenza vaccination coverage one year after the A(H1N1) influenza pandemic, France, 2010-2011.

    abstract::We report influenza vaccination coverage in target groups for the 2010-2011 influenza season, one year after the A(H1N1) pandemic. Data were collected through a one-stage cross-sectional national random telephone survey conducted in January 2011 among a sample of the population of mainland France connected to a land t...


    pub_type: 杂志文章


    authors: Guthmann JP,Fonteneau L,Bonmarin I,Lévy-Bruhl D

    更新日期:2012-02-01 00:00:00

  • Economics of polio vaccination in the post-eradication era: should OPV-using countries adopt IPV?

    abstract::The continued use of oral polio vaccine (OPV) poses a threat to polio virus eradication. Stopping all polio vaccination in the post-certification era is no longer considered to be a practical option. Policy makers agree that OPV use must stop immediately after certification. Therefore, the pragmatic alternative is for...


    pub_type: 杂志文章


    authors: Khan MM

    更新日期:2008-04-07 00:00:00

  • Is there a causal link between hepatitis B vaccination and multiple sclerosis?

    abstract::After the publication of case reports of hepatitis B vaccinees with onset or relapse of multiple sclerosis (MS), followed by a media-driven scare campaign in France, the perception that hepatitis B vaccine causes MS has developed. This has led to a fall in the acceptance of hepatitis B vaccination particularly in Fren...


    pub_type: 杂志文章


    authors: Monteyne P,André FE

    更新日期:2000-04-03 00:00:00

  • Assessment of preparation time with fully-liquid versus non-fully liquid paediatric hexavalent vaccines. A time and motion study.

    abstract:BACKGROUND AND AIMS:Simplified vaccine preparation steps would save time and reduce potential immunisation errors. The aim of the study was to assess vaccine preparation time with fully-liquid hexavalent vaccine (DTaP-IPV-HB-PRP-T, Sanofi Pasteur MSD) versus non-fully liquid hexavalent vaccine that needs reconstitution...


    pub_type: 杂志文章


    authors: De Coster I,Fournie X,Faure C,Ziani E,Nicolas L,Soubeyrand B,Van Damme P

    更新日期:2015-07-31 00:00:00

  • In search of the active PZP epitope in white-tailed deer immunocontraception.

    abstract::Native porcine zona pellucida (PZP) has been shown to be highly effective as an immunocontraceptive in white-tailed deer. However, the immunogenicity of PZP extracted from pig ovaries may vary from lot to lot and the extract has the potential of containing either viral or pathogenic material. Determination of the immu...


    pub_type: 杂志文章


    authors: Miller LA,Killian GJ

    更新日期:2002-06-21 00:00:00

  • Use of protamine sulphate for elimination of substrate DNA in poliovaccines produced on continuous cell lines.

    abstract::Cell substrate DNA was shown to be an abundant contaminant in the clarified preparations of the Sabin type 1, 2 and 3 poliovaccines produced on a continuous cell line (4647). The size of the DNA, as evaluated for the Sabin type 1 poliovaccine, was highly heterogeneous, ranging from 100 to 20,000 base pairs. In view of...


    pub_type: 杂志文章


    authors: Amosenko FA,Svitkin YV,Popova VD,Terletskaya EN,Timofeev AV,Elbert LB,Lashkevich VA,Drozdov SG

    更新日期:1991-03-01 00:00:00

  • Health and economic benefits of single-dose HPV vaccination in a Gavi-eligible country.

    abstract:BACKGROUND:Although guidelines for prophylactic human papillomavirus (HPV) vaccination recommend two doses for girls ages 9-14 years, several studies have demonstrated similar protection with one dose. Our objective was to evaluate the long-term health and economic impacts of routine one-dose HPV vaccination compared t...


    pub_type: 杂志文章


    authors: Burger EA,Campos NG,Sy S,Regan C,Kim JJ

    更新日期:2018-08-06 00:00:00

  • Serum anti-toxin B antibody correlates with protection from recurrent Clostridium difficile infection (CDI).

    abstract:BACKGROUND:Previous studies have demonstrated a correlation between Clostridium difficile anti-toxin A serum antibodies and protection against symptomatic disease and recurrence. METHODS:A neutralizing monoclonal antibody to C. difficile toxin A (CDA1) developed by MBL and Medarex, Inc. was studied in a phase II, rand...


    pub_type: 杂志文章,随机对照试验


    authors: Leav BA,Blair B,Leney M,Knauber M,Reilly C,Lowy I,Gerding DN,Kelly CP,Katchar K,Baxter R,Ambrosino D,Molrine D

    更新日期:2010-01-22 00:00:00

  • Indicators to assess National Immunization Technical Advisory Groups (NITAGs).

    abstract::A National Immunization Technical Advisory Group (NITAG) is an expert advisory committee that provides evidence-based recommendations to the Ministry of Health (MoH) to guide immunization programs and policies. The World Health Organization (WHO), the Initiative for Supporting National Independent Immunization and Vac...


    pub_type: 杂志文章


    authors: Blau J,Sadr-Azodi N,Clementz M,Abeysinghe N,Cakmak N,Duclos P,Janusz C,Jauregui B,Mihigo R,Mosina L,Takashima Y,Senouci K

    更新日期:2013-05-28 00:00:00

  • Prevention of Coxiella burnetii shedding in infected dairy herds using a phase I C. burnetii inactivated vaccine.

    abstract::The main objective of this study was to assess the efficacy of a monovalent inactivated vaccine containing phase I Coxiella burnetii to prevent Coxiella shedding in susceptible dairy cows within infected herds in comparison to a placebo. A total of 336 dairy cows and heifers, from six spontaneously infected herds, wer...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Guatteo R,Seegers H,Joly A,Beaudeau F

    更新日期:2008-08-12 00:00:00

  • Genetic immunization with GPI-anchored anthrax protective antigen raises combined CD1d- and MHC II-restricted antibody responses by natural killer T cell-mediated help.

    abstract::Studies have demonstrated that lipid rafts ultimately regulate the endocytosis of anthrax toxin via clathrin dependent pathway. Interestingly, GPI-anchored protein rich rafts have also been shown to be transported down to the endocytic pathway to reducing late endosomes. Taking advantage of this parallelism, we tried ...


    pub_type: 杂志文章


    authors: Midha S,Bhatnagar R

    更新日期:2009-03-10 00:00:00

  • A randomised vaccine field trial in Kenya demonstrates protection against wildebeest-associated malignant catarrhal fever in cattle.

    abstract::Wildebeest-associated malignant catarrhal fever (WA-MCF), a fatal disease of cattle caused by alcelaphine herpesvirus 1 (AlHV-1), is one of the most important seasonal diseases of cattle in wildebeest endemic areas, with annual incidence reaching 10%. Here we report efficacy of over 80% for a vaccine based on the atte...


    pub_type: 杂志文章


    authors: Cook E,Russell G,Grant D,Mutisya C,Omoto L,Dobson E,Lankester F,Nene V

    更新日期:2019-09-20 00:00:00

  • Oral vaccination with Lactococcus lactis expressing the Vibrio cholerae Wzm protein to enhance mucosal and systemic immunity.

    abstract::A gene associated with lipopolysaccharide (LPS) transport was cloned from a local clinical Vibrio cholerae O1 strain of the Ogawa serotype by using the Lactococcus lactis nisin-controlled expression (NICE) system. The V. cholerae wzm gene, which codes for an integral membrane transporter protein, was expressed and tar...


    pub_type: 杂志文章


    authors: Zamri HF,Shamsudin MN,Rahim RA,Neela V

    更新日期:2012-05-02 00:00:00

  • Antigen dependent and independent mechanisms that sustain serum antibody levels.

    abstract::In this article, we review three mechanisms that contribute to sustaining serum antibody levels: (i) long-lived plasma cells; (ii) persisting or cross-reactive antigen, and (iii) polyclonal activation of all memory B cells. We also present a quantitative analysis of the secondary immune response that reveals the relat...


    pub_type: 杂志文章,评审


    authors: Traggiai E,Puzone R,Lanzavecchia A

    更新日期:2003-06-01 00:00:00

  • A Rift Valley fever vaccine trial: 2. Serological response to booster doses with a comparison of intradermal versus subcutaneous injection.

    abstract::A booster dose of a formalin-inactivated, cell culture-propagated Rift Valley fever vaccine (TSI-GSD-200) was administered without significant reactogenicity to 60 volunteers 18 months after they received a three dose primary series. Blood was drawn for serological testing prior to boosting and 10, 49, and 180 days th...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Kark JD,Aynor Y,Peters CJ

    更新日期:1985-06-01 00:00:00

  • Safety and immunogenicity of an intranasal Pseudomonas aeruginosa hybrid outer membrane protein F-I vaccine in human volunteers.

    abstract::A hybrid protein [Met-Ala-(His)(6) OprF(190-342)-OprI(21-83)] consisting of the mature outer membrane protein I (OprI) and amino acids 190-342 of OprF of Pseudomonas aeruginosa was expressed in Escherichia coli and purified by Ni(2+) chelate-affinity chromatography. After several studies in healthy volunteers, as well...


    pub_type: 临床试验,杂志文章


    authors: Larbig M,Mansouri E,Freihorst J,Tümmler B,Köhler G,Domdey H,Knapp B,Hungerer KD,Hundt E,Gabelsberger J,von Specht BU

    更新日期:2001-03-21 00:00:00

  • Oral immunization with a Streptococcal pneumoniae polysaccharide conjugate vaccine in enterocoated microparticles induces serum antibodies against type specific polysaccharides.

    abstract::The authors examined the antibody responses of mice orally immunized with pneumococcal polysaccharide (type 23F) or a pneumococcal polysaccharide conjugated to the outer membrane protein complex of Neisseria meningitides (23F-OMPC). These antigens were administered either in solution or entrapped within microcapsules....


    pub_type: 杂志文章


    authors: Flanagan MP,Michael JG

    更新日期:1999-01-01 00:00:00