Secondary in vitro B lymphocyte (antibody) response to microbial antigens: use in appraisal of vaccine immunogenicity and cytokine immunoregulation.


:In order to perform preliminary evaluations of subunit vaccine candidates before extensive trials in large food-producing animals, an in vitro B-lymphocyte response assay, based on the principles of an ELISA, was established. The assay was developed in detail for the porcine system using antigens from the Gram-negative bacterium Actinobacillus pleuropneumoniae, but is shown to be applicable to other species and antigens, including viral components. It is further shown that B-cell activity in the assay is dependent on T-helper cells as well as macrophages and/or their secretory products. Thus, in addition to providing a tool for evaluation of T and B memory cell activity, the system also lends itself to dissection of T-B cell collaboration and the regulatory functions of interleukins in secondary (in vitro) antibody responses.






Ohmann HB,McDougall L,Potter A




Has Abstract


1991-03-01 00:00:00
















  • Going social: Success in online recruitment of men who have sex with men for prevention HIV vaccine research.

    abstract:OBJECTIVE:To compare the use of four different social media sites to recruit men who have sex with men (MSM) and transgender women to a phase 2b HIV prevention vaccine trial, HVTN 505. DESIGN:Retrospective, observational study. METHODS:The University of Pennsylvania HIV Vaccine Trials Unit (Penn HVTU) employed street...


    pub_type: 杂志文章


    authors: Buckingham L,Becher J,Voytek CD,Fiore D,Dunbar D,Davis-Vogel A,Metzger DS,Frank I

    更新日期:2017-06-14 00:00:00

  • Priority allocation of pandemic influenza vaccines in Australia - Recommendations of 3 community juries.

    abstract:BACKGROUND:Pandemic planning has historically been oriented to respond to an influenza virus, with vaccination strategy being a key focus. As the current COVID-19 pandemic plays out, the Australian government is closely monitoring progress towards development of SARS-CoV2 vaccines as a definitive intervention. However,...


    pub_type: 杂志文章


    authors: Degeling C,Williams J,Carter SM,Moss R,Massey P,Gilbert GL,Shih P,Braunack-Mayer A,Crooks K,Brown D,McVernon J

    更新日期:2021-01-08 00:00:00

  • Recent advances in the study of human antibody responses to influenza virus using optimized human hybridoma approaches.

    abstract::Influenza viruses exhibit a fascinating level of antigenic heterogeneity that facilitates re-infection in the human population. The human antibody repertoire also manifests endless capability for variation in the genes that specify the portion of antibody molecules interacting with epitopes. A recent explosion of tech...


    pub_type: 杂志文章


    authors: Crowe JE Jr

    更新日期:2009-12-30 00:00:00

  • Antigenicity and immunogenicity of unique prefusion-mimic F proteins presented on enveloped virus-like particles.

    abstract::Pre-fusion stabilizing mutations (DS-Cav1) in soluble fusion (F) proteins of human respiratory syncytial virus (RSV) were previously reported. Here we investigated the antigenic and immunogenic properties of pre-fusion like RSV F proteins on enveloped virus-like particles (VLP). Additional mutations were introduced to...


    pub_type: 杂志文章


    authors: Kwon YM,Lee Y,Kim KH,Jung YJ,Li Z,Jeeva S,Lee S,Moore ML,Kang SM

    更新日期:2019-10-16 00:00:00

  • Sub-optimal prevalence of mumps antibodies in a population based study of young adults in Israel after 20 years of two dose universal vaccination policy.

    abstract:BACKGROUND:A recent mumps outbreak in Israel despite an ongoing national program of a 2-dose universal childhood vaccination policy since 1988, raised questions regarding population immunity among young adults. OBJECTIVE:To assess the seroprevalence of mumps antibodies among young Israeli adults born after 1987 in ord...


    pub_type: 杂志文章


    authors: Levine H,Ankol OE,Rozhavski V,Davidovitch N,Aboudy Y,Zarka S,Balicer RD

    更新日期:2011-03-24 00:00:00

  • The immunogenicity-enhancing effect of emulsion vaccine adjuvants is independent of the dispersion type and antigen release rate--a revisit of the role of the hydrophile-lipophile balance (HLB) value.

    abstract::Effective antigen delivery is one of the most important issues in vaccine development. It has been suggested that adjuvant action results from a depot effect by prolonging the duration of the interaction between antigen and cells, and thus is related to the antigen-releasing properties of emulsion adjuvants. The objec...


    pub_type: 杂志文章


    authors: Yang YW,Wei AC,Shen SS

    更新日期:2005-04-08 00:00:00

  • Efficacy and immunogenicity of high-dose influenza vaccine in older adults by age, comorbidities, and frailty.

    abstract:BACKGROUND:A randomized trial demonstrated that a high-dose inactivated influenza vaccine (IIV-HD) was 24.2% more efficacious than a standard-dose vaccine (IIV-SD) against laboratory-confirmed influenza illness in adults ≥65 years. To evaluate the consistency of IIV-HD benefits, supplemental analyses explored efficacy ...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: DiazGranados CA,Dunning AJ,Robertson CA,Talbot HK,Landolfi V,Greenberg DP

    更新日期:2015-08-26 00:00:00

  • New neutralizing antibody epitopes in hepatitis C virus envelope glycoproteins are revealed by dissecting peptide recognition profiles.

    abstract::One of the greatest challenges to HCV vaccine development is the induction of effective immune responses using recombinant proteins or vectors. In order to better understand which vaccine-induced antibodies contribute to neutralization of HCV the quality of polyclonal anti-E1E2 antibody responses in immunized mice and...


    pub_type: 杂志文章


    authors: Kachko A,Kochneva G,Sivolobova G,Grazhdantseva A,Lupan T,Zubkova I,Wells F,Merchlinsky M,Williams O,Watanabe H,Ivanova A,Shvalov A,Loktev V,Netesov S,Major ME

    更新日期:2011-12-09 00:00:00

  • Factors influencing frontline health service providers' likelihood to recommend a future, preventive HIV vaccine to key populations in Karnataka, south India.

    abstract::The HIV epidemic in the south Indian state of Karnataka disproportionately burdens key populations of men who have sex with men and female sex workers. Despite having successfully reduced HIV incidence among certain key populations through the use of targeted intervention, India's HIV epidemic remains one of its great...


    pub_type: 杂志文章


    authors: McClarty LM,Lorway RR,Ramanaik S,Wylie J,Becker ML

    更新日期:2015-01-29 00:00:00

  • A unique algorithm for the determination of peptide-carrier protein conjugation ratio by amino acid analysis using intrinsic internal standard.

    abstract::An N-terminal peptide of the HIV-1 fusion peptide (FP) with eight amino acid residues (FP8) was conjugated to a recombinant Tetanus Toxoid Heavy Chain Fragment C (rTTHc) as a carrier protein to help boosting immunogenicity against HIV-1. In this rapid communication, a unique algorithm to determine FP-rTTHc conjugation...


    pub_type: 杂志文章


    authors: Yang GJ,Yang Y,Shaddeau A,Cai CX,Li Y,Gulla K,Zhang Y,Ou L,Cooper JW,Lei QP

    更新日期:2020-06-15 00:00:00

  • Targeted expression of HTLV-I envelope proteins in muscle by DNA immunization of mice.

    abstract::We have compared two types of plasmids for DNA immunization against HTLV-I envelope glycoproteins. One type of plasmid contains the coding DNA of the complete envelope gene of HTLV-I under the control of the CMV promoter with (CMVenvLTR) or without (CMVenv) the tax/rex genes. The second type contains the coding DNA of...


    pub_type: 杂志文章


    authors: Armand MA,Grange MP,Paulin D,Desgranges C

    更新日期:2000-04-28 00:00:00

  • Hepatitis A vaccination coverage among adolescents (13-17 years) in the United States, 2008-2016.

    abstract:BACKGROUND:The hepatitis A (HepA) vaccine was recommended by the Advisory Committee on Immunization Practices (ACIP) incrementally from 1996 to 1999. In 2006, HepA vaccine was recommended (1) universally for children aged 12-23 months, (2) for persons who are at increased risk for infection, or (3) for any person wishi...


    pub_type: 历史文章,杂志文章


    authors: Nelson NP,Yankey D,Singleton JA,Elam-Evans LD

    更新日期:2018-03-14 00:00:00

  • Antigenic differences among Newcastle disease virus strains of different genotypes used in vaccine formulation affect viral shedding after a virulent challenge.

    abstract::Strains of Newcastle disease virus (NDV) can be separated into genotypes based on genome differences even though they are antigenically considered to be of a single serotype. It is widely recognized that an efficacious Newcastle disease (ND) vaccine made with any NDV does induce protection against morbidity and mortal...


    pub_type: 杂志文章


    authors: Miller PJ,King DJ,Afonso CL,Suarez DL

    更新日期:2007-10-10 00:00:00

  • Comparison of platform technologies for assaying antibody to Ebola virus.

    abstract:BACKGROUND:The recent Ebola outbreak in West Africa led to the use of a variety of different platform technologies for assaying antibodies because of the difficulties of handling the live virus. The same types of method could be applied rapidly to other infections when they emerge. There is a need to compare quantitati...


    pub_type: 杂志文章


    authors: Wilkinson DE,Page M,Mattiuzzo G,Hassall M,Dougall T,Rigsby P,Stone L,Minor P

    更新日期:2017-03-01 00:00:00

  • Saponins, IL12 and BCG adjuvant in the FML-vaccine formulation against murine visceral leishmaniasis.

    abstract::The FML antigen of Leishmania donovani, in combination with either Riedel de Haën (R), QuilA, QS21 saponins, IL12 or BCG, was used in vaccination of an outbred murine model against visceral leishmaniasis (VL). Significant and specific increases in anti-FML IgG and IgM responses were detected for all adjuvants, and in ...


    pub_type: 杂志文章


    authors: Santos WR,de Lima VM,de Souza EP,Bernardo RR,Palatnik M,Palatnik de Sousa CB

    更新日期:2002-11-22 00:00:00

  • Vaccination with human papillomavirus type 16-derived peptides using a tattoo device.

    abstract::Tattooing has been shown to be very efficient at inducing immunity by vaccination with DNA vaccines. In this study, we examined the usability of tattooing for delivery of peptide vaccines. We compared tattooing with subcutaneous (s.c.) needle injection using peptides derived from human papillomavirus type 16 (HPV16) p...


    pub_type: 杂志文章


    authors: Pokorná D,Poláková I,Kindlová M,Dusková M,Ludvíková V,Gabriel P,Kutinová L,Müller M,Smahel M

    更新日期:2009-06-02 00:00:00

  • Efficacy of pneumococcal polysaccharide vaccine in immunocompetent adults: a meta-analysis of randomized trials.

    abstract::The use of pneumococcal polysaccharide vaccine (PPV) is low in some countries, maybe because of doubts regarding its efficacy. This meta-analysis aims at combining evidence from randomized trials of PPV assessing its efficacy in preventing Streptococcus pneumoniae related diseases in immunocompetent adults. In the fou...


    pub_type: 杂志文章,meta分析


    authors: Cornu C,Yzèbe D,Léophonte P,Gaillat J,Boissel JP,Cucherat M

    更新日期:2001-09-14 00:00:00

  • Impact of heptavalent pneumococcal conjugate vaccine on invasive disease, antimicrobial resistance and colonization in Alaska Natives: progress towards elimination of a health disparity.

    abstract::We evaluated invasive pneumococcal disease (IPD), antimicrobial resistance and nasopharyngeal colonization before and after introduction of pneumococcal conjugate vaccine (PCV7) in Alaska Natives (AN), a population with high IPD rates. We obtained IPD rates from population-based surveillance. Colonization was determin...


    pub_type: 杂志文章


    authors: Hennessy TW,Singleton RJ,Bulkow LR,Bruden DL,Hurlburt DA,Parks D,Moore M,Parkinson AJ,Schuchat A,Butler JC

    更新日期:2005-12-01 00:00:00

  • Aerosol vaccination induces robust protective immunity to homologous and heterologous influenza infection in mice.

    abstract::Live-attenuated influenza vaccine (LAIV) delivered by large droplet intranasal spray is efficacious against infection. However, many of the large droplets are trapped in the external nares and do not reach the target nasal airway tissues. Smaller droplets might provide better distribution yielding similar protection w...


    pub_type: 杂志文章


    authors: Smith JH,Brooks P,Johnson S,Tompkins SM,Custer KM,Haas DL,Mair R,Papania M,Tripp RA

    更新日期:2011-03-21 00:00:00

  • DNA vaccine against the non-structural 1 protein (NS1) of dengue 2 virus.

    abstract::Dengue is one of the most important mosquito-borne viral disease causing dengue fever and/or dengue shock syndrome/haemorrhagic fever. In some reports, the non-structural protein 1 (NS1) has been identified as a promising antigen for the development of vaccines against dengue virus (DENV). Apparently, it can elicit a ...


    pub_type: 杂志文章


    authors: Costa SM,Freire MS,Alves AM

    更新日期:2006-05-22 00:00:00

  • Enhancing the role of vaccines in combatting antimicrobial resistance.

    abstract::Interest in addressing antimicrobial resistance (AMR) has grown recently but little effort has been made to consider how existing and new vaccines could impact on AMR. A 2017 Chatham House meeting considered the role of vaccines and how to demonstrate their value through their impact on AMR. Ways existing vaccines hav...


    pub_type: 杂志文章


    authors: Clift C,Salisbury DM

    更新日期:2017-12-04 00:00:00

  • Safety of varying dosages of 7-valent pneumococcal protein conjugate vaccine in seniors previously vaccinated with 23-valent pneumococcal polysaccharide vaccine.

    abstract::In a phase I/II dose escalation study, varying volumes (0.1 ml, 0.5 ml, 1.0 ml and 2.0 ml) of 7-valent pneumococcal conjugate vaccine (PCV) (Prevnar or 0.5 ml of 23-valent pneumococcal polysaccharide vaccine (PPV) were administered to 220 adults 70 through 79 years of age previously vaccinated with 0.5 ml PPV at age 6...


    pub_type: 临床试验,杂志文章


    authors: Jackson LA,Neuzil KM,Whitney CG,Starkovich P,Dunstan M,Yu O,Nelson JC,Feikin DR,Shay DK,Baggs J,Carste B,Nahm MH,Carlone G

    更新日期:2005-05-25 00:00:00

  • Economic analysis for evidence-based policy-making on a national immunization program: a case of rotavirus vaccine in Thailand.

    abstract::Severe diarrhea caused by rotavirus is a health problem worldwide, including Thailand. The World Health Organization has recommended incorporating rotavirus vaccination into national immunization programs. This policy has been implemented in several countries, but not in Thailand where the mortality rate is not high. ...


    pub_type: 杂志文章


    authors: Muangchana C,Riewpaiboon A,Jiamsiri S,Thamapornpilas P,Warinsatian P

    更新日期:2012-04-16 00:00:00

  • Molecular shape and immunogenicity of meningococcal polysaccharide group A conjugate vaccine.

    abstract::Neisseria meningitidis is a leading cause of severe bacterial infections in infants and young children. As a major virulence factor, meningococcal capsular polysaccharide (PS) is poorly immunogenic and generally does not induce immunological memory. Conjugation of PS with a carrier protein can significantly increase t...


    pub_type: 杂志文章


    authors: Xu M,Xing X,Wu Z,Du Y,Hu T

    更新日期:2015-10-26 00:00:00

  • Expression and characterization of a novel truncated rotavirus VP4 for the development of a recombinant rotavirus vaccine.

    abstract::The outer capsid protein VP4 is an important target for the development of a recombinant rotavirus vaccine because it mediates the attachment and penetration of rotavirus. Due to the poor solubility of full-length VP4, VP8 was explored as candidate rotavirus vaccines in the past years. In previous studies, it has been...


    pub_type: 杂志文章


    authors: Li Y,Xue M,Yu L,Luo G,Yang H,Jia L,Zeng Y,Li T,Ge S,Xia N

    更新日期:2018-04-12 00:00:00

  • Shedding of live vaccine virus, comparative safety, and influenza-specific antibody responses after administration of live attenuated and inactivated trivalent influenza vaccines to HIV-infected children.

    abstract::HIV-infected children (N=243), >or=5 to <18 years old, receiving stable antiretroviral therapy, were stratified by immunologic status and randomly assigned to receive intranasal live attenuated influenza vaccine (LAIV) or intramuscular trivalent inactivated influenza vaccine (TIV). The safety profile after LAIV or TIV...


    pub_type: 杂志文章,随机对照试验


    authors: Levin MJ,Song LY,Fenton T,Nachman S,Patterson J,Walker R,Kemble G,Allende M,Hultquist M,Yi T,Nowak B,Weinberg A

    更新日期:2008-08-05 00:00:00

  • A national examination of pharmacy-based immunization statutes and their association with influenza vaccinations and preventive health.

    abstract:BACKGROUND:A series of state-level statute changes have allowed pharmacists to provide influenza vaccinations in community pharmacies. The study aim was to estimate the effects of pharmacy-based immunization statutes changes on per capita influenza vaccine prescriptions, adult vaccination rates, and the utilization of ...


    pub_type: 杂志文章


    authors: McConeghy KW,Wing C

    更新日期:2016-06-24 00:00:00

  • Chapter 15: HPV vaccine use in the developing world.

    abstract::Human papillomavirus (HPV)-related morbidity and mortality from cervical cancer primarily occurs in the developing world, where, unfortunately, access to vaccines in general, and expensive newer vaccines in particular, is often more limited than in the industrialized world. In addition, secondary prevention methods su...


    pub_type: 杂志文章,评审


    authors: Kane MA,Sherris J,Coursaget P,Aguado T,Cutts F

    更新日期:2006-08-31 00:00:00

  • BCG vaccine may generate cross-reactive T cells against SARS-CoV-2: In silico analyses and a hypothesis.

    abstract::The world is facing the rising emergency of SARS-CoV-2. The outbreak of COVID-19 has caused a global public health and economic crisis.Recent epidemiological studies have shown that a possible association of BCG vaccination program with decreased COVID-19-related risks, suggesting that BCG may provide protection again...


    pub_type: 杂志文章


    authors: Tomita Y,Sato R,Ikeda T,Sakagami T

    更新日期:2020-09-22 00:00:00

  • Recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing Ag85B-IL-7 fusion protein enhances IL-17A-producing innate γδ T cells.

    abstract::Interleukin 7 (IL-7) has an important function in the development and maintenance of IL-17A+ γδ T cells. We here constructed a recombinant Mycobacterium bovis bacillus Calmette-Guérin expressing antigen 85B (Ag85B)-IL-7 fusion protein (rBCG-Ag85B-IL-7). The Ag85B-IL-7 fusion protein and IL-7 were detected in the bacte...


    pub_type: 杂志文章


    authors: Hatano S,Tamura T,Umemura M,Matsuzaki G,Ohara N,Yoshikai Y

    更新日期:2016-05-11 00:00:00