Abstract:
:Peripheral blood mononuclear cells (PBMCs) from unvaccinated and vaccinated bovine calves were employed to study the alteration of different T-cell subpopulations, functional competence and detection of foot-and-mouth disease virus (FMDV) at different hours post in vitro infection (hpi) with FMDV serotype either O or A or Asia1. The alteration in T-lymphocyte subpopulations was analyzed by flow cytometry analysis using T-cell subpopulation specific monoclonal antibodies at various hpi. All the three FMDV serotypes down-regulate boCD4(+) and boCD8(+) T-cells up to 48hpi whereas down-regulation of boWC1(+) T-cells was observed up to 48hpi with FMDV serotype O only from unvaccinated calves. In contrast, lymphocytes from vaccinated animals demonstrated significant up-regulation of boCD4(+), boCD8(+) and boWC1(+) T-cells following exposure to FMDV serotype either O or A or Asia1. The lymphoproliferative test for functional competence of PBMCs using PHA or inactivated FMDV serotype either O or A or Asia1 antigens, revealed that PHA driven lymphoproliferative response (LPR) was significantly suppressed at 24, 48 and 72hpi in unvaccinated animals. Homotypic as well as heterotypic FMDV antigen specific LPRs were suppressed in PBMCs of unvaccinated animals infected with FMDV serotype either O or A or Asia1 and stimulated with FMDV antigens. FMDV was demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) and 3A-non-structural protein (NSP) expression in PBMCs infected in vitro with FMDV serotype either O or A or Asia1 at different hpi. Using RT-PCR, the amplified product revealed 1D gene specific product of FMDV serotype O (1301bp) or A (866bp) or Asia1 (914bp) in lymphocytes of both unvaccinated and vaccinated calves at different hpi with FMDV serotype either O or A or Asia1, respectively. Likewise, the 3A-NSP mAb based flow cytometry revealed significant increase in 3A-NSP expression in lymphocytes of unvaccinated and vaccinated calves at different hpi with FMDV serotype either O or A or Asia1. In conclusion, the present study provides significant evidence that FMDV infects PBMCs in vitro and leads to transient immuno-suppression of boCD4(+) and boCD8(+) T-cells.
journal_name
Vaccinejournal_title
Vaccineauthors
Joshi G,Sharma R,Kakker NKdoi
10.1016/j.vaccine.2009.08.107subject
Has Abstractpub_date
2009-11-12 00:00:00pages
6656-61issue
48eissn
0264-410Xissn
1873-2518pii
S0264-410X(09)01318-8journal_volume
27pub_type
杂志文章相关文献
VACCINE文献大全abstract:INTRODUCTION:As congenital cytomegalovirus (CMV) infection is one of the major causes of birth defects and developmental abnormalities, it is essential to develop vaccines and therapeutic antibodies against CMV. Clinical trials demonstrated that the subunit vaccine based on glycoprotein B, which had been believed to be...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.08.054
更新日期:2018-09-25 00:00:00
abstract::The continuously increasing demand for potent and safe vaccines and the intensifying economic pressure on health care systems underlines the need for further optimization of vaccine manufacturing. Here, we focus on downstream processing of human influenza vaccines, investigating the purification of serum-free cell cul...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.06.086
更新日期:2018-05-24 00:00:00
abstract::The full-length ecdysone receptor cDNA of Choristoneura fumiferana (CfEcR-B) was cloned into bacterial expression systems and the recombinant protein was expressed either with a His-tag (His-EcR-B) or glutathione-S-transferase (GST) fusion (GST-EcR-B). The His-EcR-B was expressed mostly as insoluble aggregates, while ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.01.050
更新日期:2006-04-12 00:00:00
abstract::The aim of the study was to compare the responses of homosexual men (HM) receiving a standard course of recombinant hepatitis B vaccine with those of healthcare workers vaccinated in the same hospital over the same period. Boosters for inadequate responders and repeat courses for non-responders were given. Forty of 61...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(03)00392-x
更新日期:2003-09-08 00:00:00
abstract::With declining influenza vaccine coverage in the elderly in recent years in France, we aimed at assessing the benefits of seasonal influenza vaccination, based on available data for observed mortality, vaccine coverage and vaccine effectiveness. To estimate the annual number of deaths avoided by vaccination in the peo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.01.023
更新日期:2015-02-25 00:00:00
abstract::A randomized, double-blind trial comparing a diphtheria-tetanus-acellular pertussis vaccine (DTaP) (pertussis toxoid and filamentous hemagglutinin) with a whole-cell vaccine (DTwP) was conducted. A case-contact study was nested in the trial to estimate absolute efficacy. From 1990 through 1994, 4181 children were rand...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0264-410x(97)00100-x
更新日期:1997-10-01 00:00:00
abstract::The objectives of this research were to evaluate the risk of prolonged testicular infection as a consequence of vaccination of peri-pubertal bulls with a modified-live, noncytopathic strain of BVDV and to assess vaccine efficacy in preventing prolonged testicular infections after a subsequent acute infection. Seronega...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.09.037
更新日期:2007-01-15 00:00:00
abstract::A new rabies vaccine was developed from Vero cells adhered to microcarriers, cultivated in a bioreactor in serum-free medium and infected with the PV/VERO-Paris rabies virus strain. The viral suspensions were concentrated by tangential filtration, purified by chromatography and inactivated with beta-propiolactone. In ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.06.014
更新日期:2004-12-09 00:00:00
abstract:BACKGROUND:Children are scheduled to receive 18-20 immunizations before their 18th birthday in England and Wales; this approximates to 13 million vaccines administered per annum. Each immunization represents a potential opportunity for immunization-related error and effective immunization is imperative to maintain the ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.06.068
更新日期:2015-07-31 00:00:00
abstract:INTRODUCTION:Replication-competent vaccinia viruses (VACVs) show prolonged antigen expression time and greater stimulation of immune responses than their replication-incompetent counterparts. However, there is the potential risk of serious post-vaccination complications, especially for children and immunocompromised in...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.05.012
更新日期:2016-06-24 00:00:00
abstract::In many countries, foot and mouth disease (FMD) is controlled by vaccination and surveillance against non-capsid proteins (NCP); therefore vaccines are required not to induce antibodies against NCP. Vaccine purity is evaluated by repeated inoculation of naïve cattle, an expensive and time consuming protocol that raise...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.05.049
更新日期:2010-09-14 00:00:00
abstract:INTRODUCTION:The strategy to Eliminate Yellow Fever Epidemics (EYE) is a global initiative that includes all countries with risk of yellow fever (YF) virus transmission. Of these, 40 countries (27 in Africa and 13 in the Americas) are considered high-risk and targeted for interventions to increase coverage of YF vaccin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.06.063
更新日期:2019-07-26 00:00:00
abstract::Brucellosis is one of the most prevalent zoonotic diseases of worldwide distribution caused by the infection of genus Brucella. Live attenuated vaccines such as B. abortus S19, B. abortus RB51 and B. melitensis Rev1 are found most effective against brucellosis infection in animals, contriving a number of serious side ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.07.106
更新日期:2014-09-29 00:00:00
abstract::Transmission-blocking vaccines against malaria parasites target molecules expressed by sexual stage parasites to elicit antibodies that prevent the infection of the mosquito vector. Pvs25 and Pvs28, expressed on the surface of ookinetes, are potential candidates for such a vaccine and induce antibodies that block the ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(01)00402-9
更新日期:2001-12-12 00:00:00
abstract:INTRODUCTION:Annual influenza vaccine coverage for young adults (including college students) remains low, despite a 2011 US recommendation for annual immunization of all people 6 months and older. College students are at high risk for influenza morbidity given close living and social spaces and extended travel during s...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.02.033
更新日期:2015-03-30 00:00:00
abstract::Hepatitis B is a parenterally and sexually transmitted disease of global importance. It associates with hepatitis D in a consistent proportion of cases. The disease most frequently runs a subclinical and anicteric course, with a significant number of cases that become chronic. Chronic hepatitis may progress to cirrhos...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/0264-410x(93)90219-n
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:China is the most populous country in the world, with an annual birth cohort of approximately 16 million, requiring an average of 500 million vaccine doses administered annually. In China, over 30 domestic and less than 10 overseas vaccine manufacturers supply over 60 licensed vaccine products, representing ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2012.11.051
更新日期:2013-01-11 00:00:00
abstract::The recent introductions of highly pathogenic avian influenza (HPAI) H5N1 virus in wild birds and its subsequent spread throughout Asia, the Middle East, Africa and Europe has put a focus on the role of wild birds in the geographical spread of HPAI H5N1 virus. Large-scale surveillance programs are ongoing to determine...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2009.02.082
更新日期:2009-10-23 00:00:00
abstract::To study the regulation of the human immune response to hepatitis B surface antigen (HBsAg) we have carefully monitored the in vivo humoral and in vitro cellular immune responses to HBsAg in 50 subjects receiving four doses of hepatitis B vaccine according to a 0, 1, 2, 12 month vaccination scheme. Twenty-three subjec...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(94)90290-9
更新日期:1994-07-01 00:00:00
abstract::In some cases of atopic dermatitis (AD), a possible pathological contribution to disease development by Candida albicans (C. albicans) has been suggested. AD patients with severe symptoms showing positive capsulated hydrolic carrier polymer radioallergosorbent test (CAP-RAST) against C. albicans demonstrated significa...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00218-9
更新日期:2002-07-26 00:00:00
abstract::Targeted delivery of vaccine candidates to the gastrointestinal (GI) tract holds potential for mucosal immunization, particularly against mucosal pathogens like the human immunodeficiency virus (HIV). Among the different strategies for achieving targeted release in the GI tract, namely the small intestine, pH sensitiv...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.10.030
更新日期:2007-12-17 00:00:00
abstract::A post hoc analysis was performed using combined data from two Phase I tolerability/immunogenicity studies of monovalent human papillomavirus type 11 (HPV11) or HPV16 L1 virus-like particle (VLP) vaccines. The goal was to determine if the HPV16 L1 VLP vaccine protected against HPV16 infection. Vaccine or placebo was g...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.vaccine.2003.11.059
更新日期:2004-07-29 00:00:00
abstract::It is of great interest to develop a pneumonic plague vaccine that would induce combined humoral and cellular immunity in the lung. Here we investigate a novel approach based on targeting of dendritic cells using the DEC-205/CD205 receptor (DEC) via the intranasal route as way to improve mucosal cellular immunity to t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.08.051
更新日期:2012-10-05 00:00:00
abstract::Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a highly contagious and often fatal disease. We have previously reported the construction and characterization of a single gene apxIIC deletion mutant HB04C(-) based on A. pleuropneumoniae serovar 7 which produces ApxII toxin and ApxIV....
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.07.053
更新日期:2007-11-01 00:00:00
abstract::Numerous evidence demonstrate Type 1 diabetes (T1D) is due to a loss of immune tolerance to islet antigens, and CD8(+) T cells play an important role in the development of T1D in NOD mice. The novel coinhibitory receptor BTLA may have a regulatory role in maintaining peripheral tolerance, however, its role in autoimmu...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.07.125
更新日期:2011-10-13 00:00:00
abstract:OBJECTIVE:Prophylactic antipyretic use during pediatric vaccination is common. This study assessed whether paracetamol or ibuprofen prophylaxis interfere with immune responses to the 13-valent pneumococcal conjugate vaccine (PCV13) given concomitantly with the combined DTaP/HBV/IPV/Hib vaccine. METHODS:Subjects receiv...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2017.02.035
更新日期:2017-04-04 00:00:00
abstract::Monoclonal antibody 3C9 was the starting material in the definition of the epitope that led to the synthesis of the first efficient peptide vaccine against a viral disease (canine parvovirus) in the natural host (dog). In this report, we have analysed the specificity of the antibody at the single amino acid level and ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(00)00526-0
更新日期:2001-03-21 00:00:00
abstract::Based on the reactivities of monoclonal antibodies, we previously reported that 25 Japanese encephalitis (JE) virus strains fell into five antigenic groups: Nakayama, Beijing-1, Kamiyama, 691004 and Muar. In the present study, we produced monoclonal antibodies against the Beijing-1 strain and obtained the Beijing-1 gr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90222-4
更新日期:1994-06-01 00:00:00
abstract::Chikungunya virus is an emerging pathogen initially found in East Africa and currently spread into the Indian Ocean Islands, many regions of South East Asia, and in the Americas. No licensed vaccines against this eminent pathogen are available and thus intensive research in this field is a priority. This review presen...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2015.05.104
更新日期:2015-07-17 00:00:00
abstract::Two cyclic peptide analogues corresponding to residues 139-146 (site A) of influenza A virus haemagglutinin (strain X31) were synthesized. The ability of these peptides to react with anti-influenza virus antibodies was found to depend on the conformation of the loop and on the orientation in which the peptide was pres...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(90)90086-2
更新日期:1990-08-01 00:00:00