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Vero-cell rabies vaccine produced using serum-free medium.

Abstract：

:A new rabies vaccine was developed from Vero cells adhered to microcarriers, cultivated in a bioreactor in serum-free medium and infected with the PV/VERO-Paris rabies virus strain. The viral suspensions were concentrated by tangential filtration, purified by chromatography and inactivated with beta-propiolactone. In immunogenicity studies performed in mice immunized with three doses of the new vaccine (seven batches) and the commercial Verorab and HDCV, mean titers of neutralizing antibodies of 10.3-34.6, 6.54 and 9.36 IU/ml were found, respectively. The vaccine presented stability during 14 months at 2-8 degrees C, 30 days at 37 degrees C and 8 h at 45 degrees C. The use of serum-free medium facilitated the downstream process leading to residual cellular DNA values <22.8 pg per dose of vaccine in all produced batches. The effective immunogenicity induced in mice by this vaccine, the degree of purity of the product, the high antigen yield and the reduction of the cost of the product due to the virus production and purification processes, makes this technology very important for countries where rabies presents a great public health problem.

journal_name

Vaccine

journal_title

Vaccine

authors

Frazatti-Gallina NM,Mourão-Fuches RM,Paoli RL,Silva ML,Miyaki C,Valentini EJ,Raw I,Higashi HG

doi

10.1016/j.vaccine.2004.06.014

subject

Has Abstract

pub_date

2004-12-09 00:00:00

pages

511-7

issue

4

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(04)00480-3

journal_volume

23

pub_type

杂志文章

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