An ESAT-6:CFP10 DNA vaccine administered in conjunction with Mycobacterium bovis BCG confers protection to cattle challenged with virulent M. bovis.


:The potency of genetic immunization observed in the mouse has demonstrated the utility of DNA vaccines to induce cell-mediated and humoral immune responses. However, it has been relatively difficult to generate comparable responses in non-rodent species. The use of molecular adjuvants may increase the magnitude of these suboptimal responses. In this study, we demonstrate that the co-administration of plasmid-encoded GM-CSF and CD80/CD86 with a novel ESAT-6:CFP10 DNA vaccine against bovine tuberculosis enhances antigen-specific cell-mediated immune responses. ESAT-6:CFP10+GM-CSF+CD80/CD86 DNA vaccinated animals exhibited significant (p<0.01) antigen-specific proliferative responses compared to other DNA vaccinates. Increased expression (p< or =0.05) of CD25 on PBMC from ESAT-6:CFP10+GM-CSF+CD80/CD86 DNA vaccinates was associated with increased proliferation, as compared to control DNA vaccinates. Significant (p<0.05) numbers of ESAT-6:CFP10-specific IFN-gamma producing cells were evident from all ESAT-6:CFP10 DNA vaccinated animals compared to control DNA vaccinates. However, the greatest increase in IFN-gamma producing cells was from animals vaccinated with ESAT-6:CFP10+GM-CSF+CD80/CD86 DNA. In a low-dose aerosol challenge trial, calves vaccinated as neonates with Mycobacterium bovis BCG and ESAT-6:CFP10+GM-CSF+CD80/CD86 DNA exhibited decreased lesion severity in the lung and lung-associated lymph nodes following viruluent M. bovis challenge compared to other vaccinated animals or non-vaccinated controls. These data suggest that a combined vaccine regimen of M. bovis BCG and a candidate ESAT-6:CFP10 DNA vaccine may offer greater protection against tuberculosis in cattle than vaccination with BCG alone.






Maue AC,Waters WR,Palmer MV,Nonnecke BJ,Minion FC,Brown WC,Norimine J,Foote MR,Scherer CF,Estes DM




Has Abstract


2007-06-11 00:00:00
















  • Improved design and intranasal delivery of an M2e-based human influenza A vaccine.

    abstract::M2 is the third integral membrane protein of influenza A. M2e, the extracellular, 23 amino acid residues of M2, has been remarkably conserved in all human influenza A strains. This prompted us to evaluate the use of M2e as a potential broad-spectrum immunogen in a mouse model for influenza infection. Genetic fusion of...


    pub_type: 杂志文章


    authors: De Filette M,Fiers W,Martens W,Birkett A,Ramne A,Löwenadler B,Lycke N,Jou WM,Saelens X

    更新日期:2006-11-10 00:00:00

  • Genetic stability of the live attenuated Bordetella pertussis vaccine candidate BPZE1.

    abstract::Despite the extensive use of efficacious pertussis vaccines, Bordetella pertussis infections are still among the main causes for childhood morbidity and mortality. Severe pertussis occurs mostly in very young children, often too young to be sufficiently protected by current vaccines, which require several administrati...


    pub_type: 杂志文章


    authors: Feunou PF,Ismaili J,Debrie AS,Huot L,Hot D,Raze D,Lemoine Y,Locht C

    更新日期:2008-10-23 00:00:00

  • Vaccine-induced host responses against very virulent Marek's disease virus infection in the lungs of chickens.

    abstract::The aim of this study was to investigate the kinetics of virus replication and cellular responses in the lungs following infection with Marek's disease virus (MDV) and/or vaccination with herpesvirus of turkey (HVT) via the respiratory route. Chickens vaccinated with HVT and challenged with MDV had a higher accumulati...


    pub_type: 杂志文章


    authors: Haq K,Abdul-Careem MF,Shanmuganthan S,Thanthrige-Don N,Read LR,Sharif S

    更新日期:2010-08-02 00:00:00

  • Targeted expression of HTLV-I envelope proteins in muscle by DNA immunization of mice.

    abstract::We have compared two types of plasmids for DNA immunization against HTLV-I envelope glycoproteins. One type of plasmid contains the coding DNA of the complete envelope gene of HTLV-I under the control of the CMV promoter with (CMVenvLTR) or without (CMVenv) the tax/rex genes. The second type contains the coding DNA of...


    pub_type: 杂志文章


    authors: Armand MA,Grange MP,Paulin D,Desgranges C

    更新日期:2000-04-28 00:00:00

  • The parasite-derived rOv-ASP-1 is an effective antigen-sparing CD4+ T cell-dependent adjuvant for the trivalent inactivated influenza vaccine, and functions in the absence of MyD88 pathway.

    abstract::Vaccination remains the most cost-effective biomedical approach for controlling influenza disease. In times of pandemics, however, these vaccines cannot be produced in sufficient quantities for worldwide use by the current manufacturing capacities and practices. What is needed is the development of adjuvanted vaccines...


    pub_type: 杂志文章


    authors: Jain S,George PJ,Deng W,Koussa J,Parkhouse K,Hensley SE,Jiang J,Lu J,Liu Z,Wei J,Zhan B,Bottazzi ME,Shen H,Lustigman S

    更新日期:2018-06-14 00:00:00

  • Characterization and optimization of a novel vaccine for protection against Lyme borreliosis.

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    pub_type: 杂志文章


    authors: Comstedt P,Hanner M,Schüler W,Meinke A,Schlegl R,Lundberg U

    更新日期:2015-11-04 00:00:00

  • Is freezing in the vaccine cold chain an ongoing issue? A literature review.

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    pub_type: 杂志文章,评审


    authors: Hanson CM,George AM,Sawadogo A,Schreiber B

    更新日期:2017-04-19 00:00:00

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    pub_type: 杂志文章


    authors: Iwasaki A,Dela Cruz CS,Young AR,Barber BH

    更新日期:1999-04-09 00:00:00

  • Expansion of syndromic vaccine preventable disease surveillance to include bacterial meningitis and Japanese encephalitis: evaluation of adapting polio and measles laboratory networks in Bangladesh, China and India, 2007-2008.

    abstract:BACKGROUND:Surveillance for acute flaccid paralysis with laboratory confirmation has been a key strategy in the global polio eradication initiative, and the laboratory platform established for polio testing has been expanded in many countries to include surveillance for cases of febrile rash illness to identify measles...


    pub_type: 杂志文章


    authors: Cavallaro KF,Sandhu HS,Hyde TB,Johnson BW,Fischer M,Mayer LW,Clark TA,Pallansch MA,Yin Z,Zuo S,Hadler SC,Diorditsa S,Hasan AS,Bose AS,Dietz V,AMES Study Group.

    更新日期:2015-02-25 00:00:00

  • Economic value of vaccinating geographically hard-to-reach populations with measles vaccine: A modeling application in Kenya.

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    pub_type: 杂志文章


    authors: Lee BY,Brown ST,Haidari LA,Clark S,Abimbola T,Pallas SE,Wallace AS,Mitgang EA,Leonard J,Bartsch SM,Yemeke TT,Zenkov E,Ozawa S

    更新日期:2019-04-17 00:00:00

  • Regulated delayed expression of rfc enhances the immunogenicity and protective efficacy of a heterologous antigen delivered by live attenuated Salmonella enterica vaccines.

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    pub_type: 杂志文章


    authors: Kong Q,Liu Q,Jansen AM,Curtiss R 3rd

    更新日期:2010-08-23 00:00:00

  • Approaches to improved influenza vaccination.

    abstract::Inactivated influenza vaccine (Ivac) has had an important impact on reducing attack rates of influenza and reducing the severity of illness amongst the vaccinees who still acquire infection. Ivac is most efficacious amongst young, otherwise healthy subjects and least effective against elderly at high risk. This is in ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Betts RF,Treanor JJ

    更新日期:2000-02-25 00:00:00

  • Rescue of a vaccine strain of peste des petits ruminants virus: In vivo evaluation and comparison with standard vaccine.

    abstract::Across the developing world peste des petits ruminants virus places a huge disease burden on agriculture, primarily affecting the production of small ruminant. The disease is most effectively controlled by vaccinating sheep and goats with live attenuated vaccines that provide lifelong immunity. However, the current va...


    pub_type: 杂志文章


    authors: Muniraju M,Mahapatra M,Buczkowski H,Batten C,Banyard AC,Parida S

    更新日期:2015-01-09 00:00:00

  • Group M consensus Gag and Nef peptides are as efficient at detecting clade A1 and D cross-subtype T-cell functions as subtype-specific consensus peptides.

    abstract:OBJECTIVE:Evaluating HIV-1 specific T-cell response in African populations is sometimes compromised by extensive virus diversity and paucity of non-clade B reagents. We evaluated whether consensus group M (ConM) peptides could serve as comparable substitutes for detecting immune responses in clade A and clade D HIV-1 i...


    pub_type: 杂志文章


    authors: Mugaba S,Nakiboneka R,Nanyonjo M,Bugembe-Lule D,Kaddu I,Nanteza B,Tweyongyere R,Kaleebu P,Serwanga J

    更新日期:2014-06-24 00:00:00

  • Horizontal transmission dynamics of a glycoprotein G deficient candidate vaccine strain of infectious laryngotracheitis virus and the effect of vaccination on transmission of virulent virus.

    abstract::Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes acute respiratory disease in chickens worldwide. The virus is horizontally transmitted and causes large outbreaks of disease. Recent studies have shown that a glycoprotein G deficient candidate vaccine strain of ILTV (ΔgG ILTV) is safe and pr...


    pub_type: 杂志文章


    authors: Devlin JM,Hartley CA,Gilkerson JR,Coppo MJ,Vaz P,Noormohammadi AH,Wells B,Rubite A,Dhand NK,Browning GF

    更新日期:2011-08-05 00:00:00

  • Variable-number tandem repeat markers for identification of Brucella abortus 82 and 75/79-AV vaccine strains.

    abstract::In Russia, live vaccine strains Brucella abortus 82 and B. abortus 75/79-AV have been widely and extensively utilized for specific prophylaxis of cattle brucellosis. To differentiate these vaccine strains from each other and laboratory collection of other vaccine (n=4), reference (n=15) and field Brucella strains (n=6...


    pub_type: 杂志文章


    authors: Kulakov YK,Zheludkov MM,Sclyarov OD

    更新日期:2010-10-01 00:00:00

  • Systematic review of the effects of pertussis vaccines in children.

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    pub_type: 杂志文章,评审


    authors: Jefferson T,Rudin M,DiPietrantonj C

    更新日期:2003-05-16 00:00:00

  • Differences in immunogenicity and vaccine potential of peptides from Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase.

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    pub_type: 杂志文章


    authors: Tallima H,Montash M,Veprek P,Velek J,Jezek J,El Ridi R

    更新日期:2003-07-04 00:00:00

  • Boosting BCG vaccination with MVA85A down-regulates the immunoregulatory cytokine TGF-beta1.

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    pub_type: 杂志文章


    authors: Fletcher HA,Pathan AA,Berthoud TK,Dunachie SJ,Whelan KT,Alder NC,Sander CR,Hill AV,McShane H

    更新日期:2008-09-26 00:00:00

  • Economic assessment of a high-dose versus a standard-dose influenza vaccine in the US Veteran population: Estimating the impact on hospitalization cost for cardio-respiratory disease.

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    pub_type: 杂志文章


    authors: van Aalst R,Russo EM,Neupane N,Mahmud SM,Mor V,Wilschut J,Chit A,Postma M,Young-Xu Y

    更新日期:2019-07-26 00:00:00

  • Long-term immunogenicity after single and booster dose of a live attenuated hepatitis A vaccine: results from 8-year follow-up.

    abstract::Live, attenuated hepatitis A vaccines are used widely in China but there is uncertainty regarding the persistence of vaccine-induced anti-HAV antibodies after single dose and booster dose administrated at month 12. A large scale clinical trial to evaluate the live, attenuated hepatitis A vaccine was conducted in Hebei...


    pub_type: 杂志文章,随机对照试验


    authors: Wang XY,Xu ZY,Ma JC,von Seidlein L,Zhang Y,Hao ZY,Han OP,Zhang YL,Tian MY,Ouyang PY,Zhang ZY,Han CQ,Xing ZC,Chen JC

    更新日期:2007-01-05 00:00:00

  • Reduction in the incidence of invasive pneumococcal disease after general vaccination with 7-valent pneumococcal conjugate vaccine in Germany.

    abstract::General vaccination with the 7-valent pneumococcal conjugate vaccine was recommended in Germany in July 2006 for all children <2 years. The proportion of reported invasive pneumococcal disease (IPD) caused by vaccine serotypes before vaccine introduction was considerably lower than in the US. We report data from natio...


    pub_type: 杂志文章


    authors: Rückinger S,van der Linden M,Reinert RR,von Kries R,Burckhardt F,Siedler A

    更新日期:2009-06-24 00:00:00

  • Transcutaneous immunization with a novel lipid-based adjuvant protects against Chlamydia genital and respiratory infections.

    abstract::Mucosal adjuvants are important to overcome the state of immune tolerance normally associated with mucosal delivery and to enhance adaptive immunity to often-weakly immunogenic subunit vaccine antigens. Unfortunately, adverse side effects of many experimental adjuvants limit the number of adjuvants approved for vaccin...


    pub_type: 杂志文章


    authors: Hickey DK,Aldwell FE,Beagley KW

    更新日期:2009-10-19 00:00:00

  • A local reaction at or near injection site: case definition and guidelines for collection, analysis, and presentation of immunization safety data.

    abstract::The need for developing a case definition and guidelines for a local reaction at or near the injection site, methods for the development of the case definition and guidelines as an adverse event following immunization as well as the rationale for selected decisions about the case definition for a local reaction at or ...


    pub_type: 指南,杂志文章


    authors: Gidudu J,Kohl KS,Halperin S,Hammer SJ,Heath PT,Hennig R,Hoet B,Rothstein E,Schuind A,Varricchio F,Walop W,Brighton Collaboration Local Reactions Working Group for a Local Reaction at or near Injection Site.

    更新日期:2008-12-09 00:00:00

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    pub_type: 杂志文章


    authors: Thompson MG,Gaglani MJ,Naleway A,Ball S,Henkle EM,Sokolow LZ,Brennan B,Zhou H,Foster L,Black C,Kennedy ED,Bozeman S,Grohskopf LA,Shay DK

    更新日期:2012-05-21 00:00:00

  • Impact of the National Perinatal Hepatitis B Prevention Programme in the Republic of Korea: A retrospective registry-based cohort study.

    abstract:INTRODUCTION:Hepatitis B is a major preventable cause of morbidity and mortality from chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. We aimed to evaluate the performance and outcomes of the Korean Perinatal Hepatitis B Prevention Programme (PHBPP) and to investigate the impact of the current post-exp...


    pub_type: 杂志文章


    authors: Yang TU,Jung CW,Kim D,Park HA,Jee Y

    更新日期:2020-07-22 00:00:00

  • Dengue tetravalent DNA vaccine increases its immunogenicity in mice when mixed with a dengue type 2 subunit vaccine or an inactivated Japanese encephalitis vaccine.

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    pub_type: 杂志文章


    authors: Imoto J,Konishi E

    更新日期:2007-01-22 00:00:00

  • Phase 1 testing of detoxified LPS/group B meningococcal outer membrane protein vaccine with and without synthetic CPG 7909 adjuvant for the prevention and treatment of sepsis.

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    pub_type: 杂志文章


    authors: Cross AS,Greenberg N,Billington M,Zhang L,DeFilippi C,May RC,Bajwa KK

    更新日期:2015-11-27 00:00:00

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    pub_type: 杂志文章


    authors: Andreotti R,Cunha RC,Soares MA,Guerrero FD,Leite FP,de León AA

    更新日期:2012-10-19 00:00:00

  • Recombinant flagellins with deletions in domains D1, D2, and D3: Characterization as novel immunoadjuvants.

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    pub_type: 杂志文章


    authors: Biedma ME,Cayet D,Tabareau J,Rossi AH,Ivičak-Kocjan K,Moreno G,Errea A,Soulard D,Parisi G,Jerala R,Berguer P,Rumbo M,Sirard JC

    更新日期:2019-01-21 00:00:00