Abstract：
:Among the numerous targets which can be used for the development of vaccines against Pseudomonas aeruginosa we focused on the outer membrane proteins OprF and OprI. The C-terminal part of OprF from aa 190 to aa 350 was investigated for its conservation and its localization of B-cell epitopes. A hybrid protein which combines the protective epitopes of OprF and OprI was expressed in E. coli and was proven to be highly protective against an intraperitoneal challenge with P. aeruginosa by active immunization of immunocompromised mice as well as by passive immunization of SCID mice with specific antisera. A purification procedure of the N-terminal His-tagged hybrid antigen was established using immobilized-metal-affinity chromatography. To evaluate its safety and immunogenicity the recombinant protein was purified for the immunization of human volunteers. The OprF/OprI hybrid protein is considered to be a candidate for a vaccine against P. aeruginosa.
journal_name
Vaccinejournal_title
Vaccineauthors
Knapp B,Hundt E,Lenz U,Hungerer KD,Gabelsberger J,Domdey H,Mansouri E,Li Y,von Specht BUdoi
10.1016/s0264-410x(98)00420-4subject
Has Abstractpub_date
1999-03-26 00:00:00pages
1663-6issue
13-14eissn
0264-410Xissn
1873-2518pii
S0264-410X(98)00420-4journal_volume
17pub_type
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