当前位置： SCI文献检索 > VACCINE期刊下所有文献 > DNA versus protein immunisation for production of monoclonal antibodies against Choristoneura fumiferana ecdysone receptor (CfEcR).

DNA versus protein immunisation for production of monoclonal antibodies against Choristoneura fumiferana ecdysone receptor (CfEcR).

Abstract：

:The full-length ecdysone receptor cDNA of Choristoneura fumiferana (CfEcR-B) was cloned into bacterial expression systems and the recombinant protein was expressed either with a His-tag (His-EcR-B) or glutathione-S-transferase (GST) fusion (GST-EcR-B). The His-EcR-B was expressed mostly as insoluble aggregates, while the GST-EcR-B was partially soluble and could be purified using affinity chromatography. Mice were then immunised with the purified GST-EcR-B protein. Due to the time-consuming protein expression and purification procedures and the solubility problem of the recombinant protein, we also inserted the full-length CfEcR-B cDNA into the mammalian DNA vaccine expression vector, pVAC1-mcs for DNA immunisation. In vitro expression of CfEcR-B in mammalian cells transfected with the pVAC-EcR-B plasmid was confirmed prior to the delivery of the DNA vaccine into mice. The anti-CfEcR-B MAbs generated from both DNA and protein vaccines were characterised and shown to recognise native CfEcR-B protein induced by 20E in CF-203 insect cells. DNA immunisation was shown to overcome the solubility problem of the bacterial expressed EcR and created a more direct route for monoclonal antibody production for this receptor protein obviating the need for EcR expression and purification to generate the antigen.

journal_name

Vaccine

journal_title

Vaccine

authors

Yang DH,Makhmoudova A,Arif BM,Feng Q,Retnakaran A,Palli SR,Krell PJ

doi

10.1016/j.vaccine.2006.01.050

subject

Has Abstract

pub_date

2006-04-12 00:00:00

pages

3115-26

issue

16

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(06)00073-9

journal_volume

24

pub_type

杂志文章

相关文献

VACCINE文献大全
  • BoLA-DR peptide binding pockets are fundamental for foot-and-mouth disease virus vaccine design in cattle.

    abstract：:This large study investigates the associations between bovine major histocompatibility complex DRB3 alleles and their binding pockets with the immune response to a 40-mer peptide derived from foot-and-mouth disease virus (FMDV) VP1. A crossbred (Charolais and Holstein) cattle population (n=197) was immunised with the ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.09.131

    authors： Baxter R,Craigmile SC,Haley C,Douglas AJ,Williams JL,Glass EJ

    更新日期：2009-12-10 00:00:00

  • Interchangeability and tolerability of two inactivated hepatitis A vaccines in Chinese children.

    abstract：:In China, no data are available to evaluate the interchangeability between Chinese domestic inactivated hepatitis A vaccines (Healive) and imported inactivated hepatitis A vaccines (Havrix). A double-blind, randomized controlled study was to compare interchangeability and safety of Healive and Havrix among Chinese chi...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2012.04.038

    authors： Zhang ZL,Zhu XJ,Wang X,Liang M,Sun J,Liu Y,Gao ZG,Wu JY,Dong XJ,Liu RK,Chen JT,Zhang YQ,Wang W,Zhang LP,Yin W

    更新日期：2012-06-08 00:00:00

  • Evaluation of novel Brucella melitensis unmarked deletion mutants for safety and efficacy in the goat model of brucellosis.

    abstract：:Pregnant goats were employed to assess unmarked deletion mutant vaccine candidates BMDeltaasp24, BMDeltacydBA, and BMDeltavirB2, as the target host species naturally infected with Brucella melitensis. Goats were assessed for the degree of pathology associated with the vaccine strains as well as the protective immunity...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2006.04.005

    authors： Kahl-McDonagh MM,Elzer PH,Hagius SD,Walker JV,Perry QL,Seabury CM,den Hartigh AB,Tsolis RM,Adams LG,Davis DS,Ficht TA

    更新日期：2006-06-12 00:00:00

  • Evaluation of 12 stabilizers in a developed attenuated Salmonella Enteritidis vaccine.

    abstract：:The development of a stable live attenuated Salmonella Enteritidis (SE) vaccine, resisting heat stress during transportation and storage in unequipped tropical and subtropical zones of the world, is highly recommended. Twelve stabilizers were individually supplemented into a 9 ml volume of sterile distilled water resu...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(02)00107-x

    authors： Barbour EK,Abdelnour A,Jirjis F,Faroon O,Farran MT

    更新日期：2002-05-22 00:00:00

  • Induction of a secretory IgA response in the murine female urogenital tract by immunization of the lungs with liposome-supplemented viral subunit antigen.

    abstract：:This study demonstrates that liposomes administered to the lower respiratory tract of mice have the capacity to stimulate secretory IgA (s-IgA) antibody production in the female urogenital system. Total respiratory tract immunization of mice with influenza virus subunit antigen simply mixed with negatively charged lip...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(94)00062-r

    authors： de Haan A,Renegar KB,Small PA Jr,Wilschut J

    更新日期：1995-05-01 00:00:00

  • Development of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens.

    abstract：:The objective of the present study was to investigate the feasibility of a subunit vaccine and a live bacteria vaccine to protect chickens against infectious bursal disease virus (IBDV) infection. The gene for VP2 of a new wild-type very virulent IBDV (vvIBDV) strain was cloned into an Escherichia coli expression syst...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.05.015

    authors： Rong J,Cheng T,Liu X,Jiang T,Gu H,Zou G

    更新日期：2005-09-23 00:00:00

  • Amount of maternal rotavirus-specific antibodies influence the outcome of rotavirus vaccination of newborn mice with virus-like particles.

    abstract：:In presence of low or high levels of rotavirus-specific maternal antibodies, the ability of newborn mice to respond to immunization with rotavirus RF 8*-2/6/7 VLPs, was evaluated. After parenteral vaccination, 100% of offspring born to low-antibody-titer dams developed rotavirus-specific IgG antibodies (n=7). In contr...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.11.089

    authors： Johansson E,Istrate C,Charpilienne A,Cohen J,Hinkula J,Poncet D,Svensson L,Johansen K

    更新日期：2008-02-06 00:00:00

  • Antigen-specific IgA titres after 23-valent pneumococcal vaccine indicate transient antibody deficiency disease in children.

    abstract：:Paediatric patients with antibody deficiency may either be delayed in development of humoral immunity or may be persistently deficient in antibody production. To differentiate between these entities, we examined the 23-valent pneumococcal polysaccharide (PnPS) vaccine-induced IgM-, IgG- and IgA antibody responses in a...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.09.041

    authors： Janssen WJ,Nierkens S,Sanders EA,Boes M,van Montfrans JM

    更新日期：2015-11-17 00:00:00

  • Effect of different adjuvant formulations on the immunogenicity and protective effect of a live Mycoplasma hyopneumoniae vaccine after intramuscular inoculation.

    abstract：:Mycoplasma hyopneumoniae (M. hyopneumoniae) vaccine strain 168 is an intrapulmonically injected attenuated live vaccine that is available in the Chinese market. The aim of this study was to develop suitable adjuvants for this live vaccine to provide effective protection after intramuscular inoculation. Several adjuvan...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2014.03.071

    authors： Xiong Q,Wei Y,Xie H,Feng Z,Gan Y,Wang C,Liu M,Bai F,Xie F,Shao G

    更新日期：2014-06-05 00:00:00

  • Protective and disease-enhancing immune responses induced by recombinant modified vaccinia Ankara (MVA) expressing respiratory syncytial virus proteins.

    abstract：:Modified vaccinia Ankara (MVA) recombinants expressing single or multiple RSV surface proteins (F or G) are promising potential vaccines. We studied humoral and cellular responses induced by MVA-F and MVA-G in mice, comparing them to a formalin inactivated RSV preparation (FI-RSV) known to increase disease severity. M...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2004.05.015

    authors： Olszewska W,Suezer Y,Sutter G,Openshaw PJ

    更新日期：2004-11-25 00:00:00

  • Measles, mumps, and rubella antibodies in children 5-6 years after immunization: effect of vaccine type and age at vaccination.

    abstract：:The levels of antibody against measles, mumps, and rubella were determined at 5-6 years postimmunization in 468 children vaccinated with two different trivalent vaccines. The proportions of children without detectable antibody levels were 12 and 3.6% for measles (p < 0.001), 14.9 and 7% for mumps (p = 0.006), and 3.3 ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(95)00098-l

    authors： Boulianne N,De Serres G,Ratnam S,Ward BJ,Joly JR,Duval B

    更新日期：1995-11-01 00:00:00

  • Optimal approaches to data collection and analysis of potential immune mediated disorders in clinical trials of new vaccines.

    abstract：BACKGROUND:The potential for development of autoimmune diseases after vaccination with new vaccines containing novel adjuvants is a theoretical concern. Randomised, placebo-controlled trials are the best method for assessing a potential causal relationship between an adverse event and vaccination, but usually have a sa...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2013.01.042

    authors： Tavares Da Silva F,De Keyser F,Lambert PH,Robinson WH,Westhovens R,Sindic C

    更新日期：2013-04-03 00:00:00

  • Enhanced immune sera and vaccine: safe approach to treat scorpion envenoming.

    abstract：:Irradiation of Androctonus australis hector venom using a dose of 2 kGy has successfully abolished toxicity without reducing its antigenic or immunogenic properties. Toxicity of irradiated antigen was abolished until 20 times of LD(50) of native venom. Analysis of physiopathological effects induced by native and irrad...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.09.044

    authors： Lila BA,Laraba-Djebari F

    更新日期：2011-11-08 00:00:00

  • Immunogenic virus-like particles continuously expressed in mammalian cells as a veterinary rabies vaccine candidate.

    abstract：:Rabies is one of the most lethal infectious diseases in the world, with a mortality approaching 100%. There are between 60,000 and 70,000 reported annual deaths, but this is probably an underestimation. Despite the fact that there are vaccines available for rabies, there is a real need of developing more efficacious a...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.03.088

    authors： Fontana D,Kratje R,Etcheverrigaray M,Prieto C

    更新日期：2015-08-20 00:00:00

  • The role of the vaccines industry in Mission Grand Convergence.

    abstract：:The vast majority of vaccines used throughout the world are supplied by the private sector. It is essential therefore that the industry is closely engaged in future policy developments at a national and international level and is able to respond to the changing needs and priorities that may be required to ensure the s...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2016.10.084

    authors： Almond J,Medaglini D

    更新日期：2017-01-20 00:00:00

  • Influenza A vaccines using linear expression cassettes delivered via electroporation afford full protection against challenge in a mouse model.

    abstract：:Alternative DNA vaccine constructs such as fully synthetic linear expressing cassettes (LECs) offer the advantage of accelerated manufacturing techniques as well as the lack of both antibiotic resistance genes and bacterial contaminants. The speed of manufacture makes LEC technology a possible future vaccination strat...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.02.071

    authors： Shen X,Söderholm J,Lin F,Kobinger G,Bello A,Gregg DA,Broderick KE,Sardesai NY

    更新日期：2012-11-06 00:00:00

  • Effectiveness of Lanzhou lamb rotavirus vaccine against rotavirus gastroenteritis requiring hospitalization: a matched case-control study.

    abstract：:We sought to determine the vaccine effectiveness (VE) of Lanzhou lamb rotavirus (LLR) vaccine against rotavirus gastroenteritis in children <5 requiring hospitalization. Children hospitalized 2002-2004 with rotavirus gastroenteritis were matched by gender, age and community to 838 controls. VE was calculated for one o...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.10.036

    authors： Fu C,Wang M,Liang J,He T,Wang D,Xu J

    更新日期：2007-12-17 00:00:00

  • A nationwide vaccination programme in Italy against hepatitis B virus infection in infants of hepatitis B surface antigen-carrier mothers.

    abstract：:In order to prevent hepatitis B virus (HBV) infection of infants of hepatitis B surface antigen (HBsAg)-positive mothers, a nation-wide immunization programme was initiated in Italy in January 1984. During the first 3 years (1984-86), 651,667 out of 1726,000 pregnant women (37.8%) were screened for HBsAg; the percenta...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/0264-410x(89)90056-x

    authors： Stroffolini T,Pasquini P,Mele A

    更新日期：1989-04-01 00:00:00

  • Observational study of vaccine effectiveness 20 years after the introduction of universal hepatitis B vaccination in Tunisia.

    abstract：OBJECTIVES:The objectives of this study were to estimate the national prevalence of hepatitis B infection in Tunisia using data from a nationwide survey, to compare results with those obtained in 1996 survey and to evaluate the impact of vaccination twenty years after its introduction. METHODS:A National household-bas...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.08.038

    authors： Ben Hadj M,Bouguerra H,Saffar F,Chelly S,Hechaichi A,Talmoudi K,Bahrini A,Chouki T,Hazgui O,Hannachi N,Letaief H,Bellali H,Bahri O,Ben-Alaya-Bouafif N

    更新日期：2018-09-18 00:00:00

  • Durability of antibody responses after receipt of the monovalent 2009 pandemic influenza A (H1N1) vaccine among HIV-infected and HIV-uninfected adults.

    abstract：BACKGROUND:Human immunodeficiency virus (HIV)-infected persons are at risk for severe influenza infections. Although vaccination against the H1N1 pandemic influenza strain is recommended, currently there are no data on the durability of post-vaccination antibody responses in this population. METHODS:HIV-infected and H...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.02.040

    authors： Crum-Cianflone NF,Iverson E,Defang G,Blair PJ,Eberly LE,Maguire J,Ganesan A,Faix D,Duplessis C,Lalani T,Whitman T,Brandt C,Macalino G,Millar EV,Burgess T

    更新日期：2011-04-12 00:00:00

  • Protection against experimental bubonic and pneumonic plague by a recombinant capsular F1-V antigen fusion protein vaccine.

    abstract：:The current human whole-cell vaccine is ineffective against pneumonic plague caused by typical F1 capsule positive (F1+) strains of Yersinia pestis. The authors found this vaccine to also be ineffective against F1-negative (F1-) Y. pestis strains, which have been isolated from a human case and from rodents. For these ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(98)80110-2

    authors： Heath DG,Anderson GW Jr,Mauro JM,Welkos SL,Andrews GP,Adamovicz J,Friedlander AM

    更新日期：1998-07-01 00:00:00

  • Full genome based sequence and structural characterization of an unusual group A rotavirus G12P[11] isolated from neonates in Pune, western India.

    abstract：:Studies conducted at neonatal intensive care units in Pune, western India, suggested early exposure to rotaviruses and predominance of unusual human-bovine-like G12P[11] strains. The whole genome sequencing and phylogenetic analyses of a naturally attenuated, culture adapted neonatal strain, (NIV-1740121) revealed mul...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2020.01.081

    authors： Ranshing SS,Cherian SS,Agarwal MS,Jagtap AS,Patil CM,Gopalkrishna V

    更新日期：2020-02-28 00:00:00

  • Mutant influenza A virus nucleoprotein is preferentially localized in the cytoplasm and its immunization in mice shows higher immunogenicity and cross-reactivity.

    abstract：:Many influenza vaccines targeted to hemagglutinin (HA) show efficient immunogenicity for protecting subjects against influenza virus infection. Major antigenic changes to HA molecules can help influenza virus to develop resistance against HA-targeted vaccines. DNA vaccines encoding conserved antigens protect animals a...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2007.02.074

    authors： Ohba K,Yoshida S,Zahidunnabi Dewan M,Shimura H,Sakamaki N,Takeshita F,Yamamoto N,Okuda K

    更新日期：2007-05-22 00:00:00

  • Streptococcus pneumoniae pep27 mutant as a live vaccine for serotype-independent protection in mice.

    abstract：:Streptococcus pneumoniae (pneumococcus) is responsible for significant morbidity and mortality in worldwide. After introduction of current pneumococcal vaccines, a marked decrease in the incidence of pneumococcal disease was observed. Unfortunately, serotype shifts in carriage and disease, including capsular switch an...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.11.073

    authors： Kim EH,Choi SY,Kwon MK,Tran TD,Park SS,Lee KJ,Bae SM,Briles DE,Rhee DK

    更新日期：2012-03-02 00:00:00

  • Development of an ex vivo human skin model for intradermal vaccination: tissue viability and Langerhans cell behaviour.

    abstract：:The presence of resident Langerhans cells (LCs) in the epidermis makes the skin an attractive target for DNA vaccination. However, reliable animal models for cutaneous vaccination studies are limited. We demonstrate an ex vivo human skin model for cutaneous DNA vaccination which can potentially bridge the gap between ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.07.088

    authors： Ng KW,Pearton M,Coulman S,Anstey A,Gateley C,Morrissey A,Allender C,Birchall J

    更新日期：2009-10-09 00:00:00

  • Evaluation of a single dose of half strength inactivated influenza vaccine in healthy adults.

    abstract：:Because of delays in the manufacturing of the 2000-2001, trivalent inactivated influenza vaccine in the US, there were concerns that there might be shortages of vaccine supply in the US. Therefore, we conducted a prospective, randomized, open-label, multicenter trial at six academic medical centers in the US, to evalu...

    journal_title：Vaccine

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1016/s0264-410x(01)00440-6

    authors： Treanor J,Keitel W,Belshe R,Campbell J,Schiff G,Zangwill K,Wolff M,Klimov A,Levandowski R,Lambert L

    更新日期：2002-01-15 00:00:00

  • Impact and cost effectiveness of pneumococcal conjugate vaccine in India.

    abstract：BACKGROUND:World Health Organization has recommended the introduction of pneumococcal conjugate vaccine (PCV) in the childhood immunisation programme of all the countries in the world. In lieu of its introduction in India, there is a need to generate evidence on cost-effectiveness of this vaccine. The current study loo...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.12.004

    authors： Krishnamoorthy Y,Eliyas SK,Nair NP,Sakthivel M,Sarveswaran G,Chinnakali P

    更新日期：2019-01-21 00:00:00

  • The fight against rabies in Africa: From recognition to action.

    abstract：:As a follow-up to the first AfroREB meeting, held in Grand Bassam (Côte d'Ivoire) in March 2008, African rabies experts of the AfroREB network met a second time to complete the evaluation of the rabies situation in Africa and define specific action plans. About 40 French speaking rabies specialists from northern, west...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.06.030

    authors： Dodet B,Africa Rabies Bureau (AfroREB).

    更新日期：2009-08-13 00:00:00

  • Do not underestimate the power of antibodies--lessons from adoptive transfer of antibodies against HIV.

    abstract：:Successes for neutralizing antibodies (nAbs) against the human immunodeficiency virus (HIV) include potent cross-clade neutralization of primary virus isolates by human neutralizing monoclonal antibodies (nmAbs) targeting conserved envelope epitopes. Furthermore, passively administered combinations of human nmAbs prev...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/s0264-410x(02)00389-4

    authors： Ferrantelli F,Rasmussen RA,Hofmann-Lehmann R,Xu W,McClure HM,Ruprecht RM

    更新日期：2002-12-19 00:00:00

  • Immunogenicity and protective efficacy of a novel foot-and-mouth disease virus empty-capsid-like particle with improved acid stability.

    abstract：:Foot-and-mouth disease virus (FMDV) is the etiological agent of a highly contagious disease that affects cloven-hoofed animal species. The FMDV capsid is highly acid labile and viral particles lose their immunogenicity when they disassemble at mildly acidic pHs. The viral capsid of FMDV serotype O is more sensitive th...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2019.02.032

    authors： Xie Y,Li H,Qi X,Ma Y,Yang B,Zhang S,Chang H,Yin X,Li Z

    更新日期：2019-03-28 00:00:00