Abstract:
:Monoclonal antibody 3C9 was the starting material in the definition of the epitope that led to the synthesis of the first efficient peptide vaccine against a viral disease (canine parvovirus) in the natural host (dog). In this report, we have analysed the specificity of the antibody at the single amino acid level and the contribution of each residue to the binding, using multiple length analysis. Moreover, a replacement analysis allowed determining those critical residues for the binding. Finally, in an attempt to optimise the production cost of the vaccine, we have determined that the minimal dose required for induction of protective antibodies can be as low as 0.5 microg of peptide. Also, KLH can be replaced as a carrier for a much cheaper alternative such as ovalbumine. All these findings implicate a substantial reduction in the cost of the vaccinal dose.
journal_name
Vaccinejournal_title
Vaccineauthors
Langeveld JP,Martinez-Torrecuadrada J,Boshuizen RS,Meloen RH,Ignacio Casal Jdoi
10.1016/s0264-410x(00)00526-0subject
Has Abstractpub_date
2001-03-21 00:00:00pages
2352-60issue
17-19eissn
0264-410Xissn
1873-2518pii
S0264410X00005260journal_volume
19pub_type
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