Abstract:
:The aim of current study was to evaluate the prospects of somatostatin DNA vaccine. Two copies of somatostatin (SS) genes were fused with the hepatitis B surface antigen (HBsAg) S gene using genetic engineering methods, the identified recombinant plasmid designated as pcS/2SS was transfected into HeLa cells to detect expression and antigenicity of target fusion protein, and its immunoreaction as well as safety was evaluated with animal experiments. The expressed target protein had a specific reaction with somatostatin antibody and showed a single strip result. A single injection of this vector stimulated long-term antigen-specific antibody responses in rats, and peak antibody levels occurred at the 2nd week of the initial injection. Additionally, the 50 microg immunized group resulted in a 13.5% increase in growth rate as compared with control group (111.7 g vs. 98.4 g). The genomic DNA was assayed for integrated plasmid using a sensitive PCR method, and the risk of mutation due to integration of pcS/2SS plasmid following intramuscular injection in mice was negligible. The successful construction of pcS/2SS DNA vaccine with good immunogenicity and safety has prospects to promote growth of animals.
journal_name
Vaccinejournal_title
Vaccineauthors
Liang A,Cao S,Han L,Yao Y,Moaeen-Ud-Din M,Yang Ldoi
10.1016/j.vaccine.2008.03.036subject
Has Abstractpub_date
2008-06-02 00:00:00pages
2935-41issue
23eissn
0264-410Xissn
1873-2518pii
S0264-410X(08)00375-7journal_volume
26pub_type
杂志文章相关文献
VACCINE文献大全abstract::The identification of new vaccine candidates for leptospirosis is of great interest. This paper describes the identification of two homologous immunogenic proteins, LigA-m and LigB-m, of Leptospira interrogans serovar manilae strain UP-MMC-NIID, which show strong similarity with LigA found recently in L. interrogans s...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.10.007
更新日期:2004-03-29 00:00:00
abstract::A fusion protein, comprising the B subunit of the heat-labile enterotoxin and a portion of the precursor to the heat-stable enterotoxin of Escherichia coli, has been created by recombinant genetic techniques. It is exported successfully to the bacterial periplasm and assembles into pentamers which retain the ability t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90022-p
更新日期:1993-01-01 00:00:00
abstract::H5N1, highly pathogenic avian influenza poses, a threat to animal and human health. Rapid changes in H5N1 viruses require periodic reformulation of the conventional strain-matched vaccines, thus emphasizing the need for a broadly protective influenza vaccine. Here, we constructed BV-Dual-3M2e-LTB, a recombinant baculo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.12.039
更新日期:2016-01-27 00:00:00
abstract::This study examined the effect of congestive heart failure (CHF) on immune responses to influenza vaccination (2000-2001 preparation) in three groups of older adults including healthy, Class II and Class III/IV CHF. Serum antibody titers measured by hemagglutination inhibition (HI), and interferon-gamma (IFN-gamma), i...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.08.032
更新日期:2004-01-26 00:00:00
abstract::We previously developed a dengue tetravalent DNA vaccine that can induce neutralizing antibodies against four dengue viruses in mice. Here, we demonstrated that immunogenicity of our tetravalent vaccine is synergistically increased in mice by co-immunization with dengue type 2 virus (DENV2) subviral extracellular part...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.09.059
更新日期:2007-01-22 00:00:00
abstract::The authors trace the origins and history of anthrax and anthrax vaccines. They describe the aetiology and pathogenesis of the disease and the variety of symptoms which result from infection. The authors relate the early work performed by Pasteur, the development of existing vaccines and the efficacy of these vaccines...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/0264-410x(84)90003-3
更新日期:1984-06-01 00:00:00
abstract::Efficacy studies of investigational HIV vaccines require enrollment of individuals at 'high risk' for HIV. This paper examines participation in HIV vaccine trials among women at 'high risk' for HIV acquisition. In-depth interviews were conducted with 17 African-American women who use crack cocaine and/or exchange sex ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.06.064
更新日期:2011-08-18 00:00:00
abstract::In this work, the propagation of the 17DD yellow fever virus in Vero cells grown on Cytodex-1 microcarriers was evaluated. After verifying that upon infection the virus adsorption step could be performed under continuous agitation, experiments were carried out in spinners and sparged lab-scale stirred-tank bioreactor ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.06.023
更新日期:2009-10-30 00:00:00
abstract::NetB (necrotic enteritis toxin B) is a recently identified β-pore-forming toxin produced by Clostridium perfringens. This toxin has been shown to play a major role in avian necrotic enteritis. In recent years, a dramatic increase in necrotic enteritis has been observed, especially in countries where the use of antimic...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.05.063
更新日期:2013-08-20 00:00:00
abstract::Capsular polysaccharides are important virulence factors of Streptococcus pneumoniae. The polysaccharide has been used as a component of vaccines against pneumococcal diseases either as plain polysaccharide or better conjugated to a protein. The last one is the vaccine of choice to target child protection. The immune ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.09.047
更新日期:2012-11-19 00:00:00
abstract::The efficacy of immunization of a vaccine depends on an antigen, an adjuvant, and on the expression of multiple genes in the host. The responsiveness of various strains of mice to adjuvants is therefore dependent on their genetic background present as a complex, multigenic trait similarly as in man. We have recently d...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00528-5
更新日期:2003-02-14 00:00:00
abstract::Rams which were vaccinated at 6-8 months of age with a water-in-oil Brucella ovis-vitamin E adjuvant vaccine had significantly higher serum antibody levels than rams vaccinated with a commercial B. ovis bacterin or B. melitensis Rev 1. The adjuvant vaccine did not cause abscesses at the site of injection as some water...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(91)90111-i
更新日期:1991-04-01 00:00:00
abstract::Group A rotavirus (RVA) is a major cause of diarrhea and diarrhea-related mortality in foals in parts of the world. In addition to careful horse farm management, vaccination is the only known alternative to reduce the RVA associated disease burden on horse farms. The precise evaluation of vaccine effectiveness against...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2013.08.045
更新日期:2013-11-19 00:00:00
abstract:BACKGROUND:British Columbia (BC) introduced a school-based HPV vaccine program in September 2008. As part of the HPV vaccine program evaluation, we determined the type-specific HPV prevalence in a population-based sample of women presenting for routine cervical cancer screening in the province. METHODS:From June 2010 ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.09.085
更新日期:2013-02-04 00:00:00
abstract:BACKGROUND:Measles is a highly infectious respiratory disease which causes 122,000 deaths annually. Although measles vaccine is extremely safe and effective, vaccine coverage could be improved by a vaccine that is more easily administered and transported. We developed an inhalable dry powder measles vaccine (MVDP) and ...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2014.09.071
更新日期:2014-11-28 00:00:00
abstract::New and improved vaccines and delivery systems are increasingly being developed for prevention, treatment and diagnosis of human diseases. Prior to their use in humans, all new biological products must undergo pre-clinical evaluation. These pre-clinical studies are important not only to establish the biological proper...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2005.01.135
更新日期:2006-04-12 00:00:00
abstract::It has been hypothesized that antibody induced by Plasmodium falciparum circumsporozoite protein vaccine would be effective against endemic human malaria. In a malaria endemic region of Kenya, 76 volunteers, in 38 pairs sleeping adjacently, were immunized with subunit circumsporozoite protein Asn-Ala-Asn-Pro tetrapept...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(95)00221-l
更新日期:1996-06-01 00:00:00
abstract:BACKGROUND:Surveillance for acute flaccid paralysis with laboratory confirmation has been a key strategy in the global polio eradication initiative, and the laboratory platform established for polio testing has been expanded in many countries to include surveillance for cases of febrile rash illness to identify measles...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.01.004
更新日期:2015-02-25 00:00:00
abstract::A reassortant virus possessing RNA segment 7, which codes for the M1 and M2 proteins, of the avian influenza A/Mallard/New York/6750/78 (H2N2) virus and the other seven RNA segments of the human influenza A/Udorn/307/72 (H3N2) virus had been shown previously to be markedly restricted in replication in the respiratory ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(89)90283-1
更新日期:1989-12-01 00:00:00
abstract::The mechanisms of protection elicited by smallpox vaccine in humans have not been delineated. Dendritic cells (DCs) are the most potent of professional antigen presenting cells which on activation modulate both innate and adaptive immune responses. Immunogenic proteins of vaccinia virus have been identified with prote...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.10.031
更新日期:2009-01-01 00:00:00
abstract::Human intestinal infections with the nematode Strongyloides stercoralis remain a significant problem worldwide and a vaccine would be a useful addition to the tools available to prevent and control this infection. The goal of this study was to test single antigens for their efficacy in a vaccine against S. stercoralis...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.08.030
更新日期:2011-10-19 00:00:00
abstract::Trehalose-6,6'-dimycolate (TDM) and its monosaccharide-type analogues were synthesized, and their lethal and adjuvant activities were examined in mice. All the monosaccharide-type analogues with a glucose or N-acetylglucosamine moiety were devoid of lethal toxicity to mice; in particular, D-GlcNAcM(1-deoxy) and D-GlcN...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(89)90242-9
更新日期:1989-06-01 00:00:00
abstract::Ethnicity may be associated with the incidence of pneumococcal infections and the frequency of protective vaccine responses. Earlier studies have suggested that HIV-infected persons of black ethnicity develop less robust immune responses to pneumococcal vaccination that may relate to their higher incidence of invasive...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.09.056
更新日期:2010-11-10 00:00:00
abstract::Dengue virus (DENV) is the causal agent of severe disease and, in some cases, mortality in humans, but no licensed vaccines against dengue are available. An effective vaccine against dengue requires long-term humoral and cellular immune responses. Several viral proteins have been the subjects of intense research, espe...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.03.009
更新日期:2013-05-01 00:00:00
abstract::The amino terminal region of group A streptococcal M proteins evokes type-specific immunity while the conserved C-repeat epitopes evoke cross-protective immunity against multiple serotypes. The present studies were undertaken to compare the protective efficacy of vaccines containing either type-specific (hexavalent va...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.05.018
更新日期:2010-07-12 00:00:00
abstract:BACKGROUND:We have developed a new oral vaccine against enterotoxigenic Escherichia coli (ETEC), which is the most common cause of bacterial diarrhea in children in developing countries and in travelers. METHODS:The vaccine was tested for safety and immunogenicity alone and together with double-mutant heat-labile toxi...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2014.10.069
更新日期:2014-12-12 00:00:00
abstract:OBJECTIVE:Evaluating HIV-1 specific T-cell response in African populations is sometimes compromised by extensive virus diversity and paucity of non-clade B reagents. We evaluated whether consensus group M (ConM) peptides could serve as comparable substitutes for detecting immune responses in clade A and clade D HIV-1 i...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.05.021
更新日期:2014-06-24 00:00:00
abstract:BACKGROUND:Bacillus Calmette-Guérin (BCG) vaccination is a widely-used public health intervention for tuberculosis (TB) control. In Taiwan, like other intermediate TB burden settings, steadily declining TB incidence raises important questions on whether universal BCG vaccination should be discontinued. Recent surveys o...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.08.019
更新日期:2018-09-18 00:00:00
abstract::Human metapneumovirus (HMPV) is a major cause of morbidity and mortality from acute lower respiratory tract illness, with most individuals seropositive by age five. Despite the presence of neutralizing antibodies, secondary infections are common and can be severe in young, elderly, and immunocompromised persons. Precl...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.04.034
更新日期:2016-05-23 00:00:00
abstract::Hepatitis A caused by hepatitis A virus (HAV) transmitted by the fecal-oral route, results in considerable morbidity and economic loss. Mucosal immunization can be more effective than conventional injection at inducing both local and systemic immunity to HAV. Here we show that co-administration of killed HAV with synt...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.04.015
更新日期:2006-06-19 00:00:00