Recombinant enterotoxins as vaccines against Escherichia coli-mediated diarrhoea.


:A fusion protein, comprising the B subunit of the heat-labile enterotoxin and a portion of the precursor to the heat-stable enterotoxin of Escherichia coli, has been created by recombinant genetic techniques. It is exported successfully to the bacterial periplasm and assembles into pentamers which retain the ability to bind to GM1 ganglioside. Native toxin epitopes are displayed and the molecule can be easily purified from periplasmic extracts of cells expressing the gene fusion. Although the protein carries the natural sequence of the heat-stable enterotoxin, it is greatly attenuated in toxicity. Systemic immunization of mice or oral administration of the fusion elicits antibody responses against both classes of E. coli enterotoxin.






Aitken R,Hirst TR




Has Abstract


1993-01-01 00:00:00
















  • Outer membrane vesicles derived from Bordetella parapertussis as an acellular vaccine against Bordetella parapertussis and Bordetella pertussis infection.

    abstract::Bordetella parapertussis, a close related species of B. pertussis, can also cause the disease named pertussis or whooping cough. The number of cases caused by this related pathogen has risen sustained in the last years. The widely used cellular (wP) or acellular (aP) pertussis vaccines have little or no efficacy again...


    pub_type: 杂志文章


    authors: Bottero D,Gaillard ME,Errea A,Moreno G,Zurita E,Pianciola L,Rumbo M,Hozbor D

    更新日期:2013-10-25 00:00:00

  • Placebo use in vaccine trials: recommendations of a WHO expert panel.

    abstract::Vaccines are among the most cost-effective interventions against infectious diseases. Many candidate vaccines targeting neglected diseases in low- and middle-income countries are now progressing to large-scale clinical testing. However, controversy surrounds the appropriate design of vaccine trials and, in particular,...


    pub_type: 杂志文章


    authors: Rid A,Saxena A,Baqui AH,Bhan A,Bines J,Bouesseau MC,Caplan A,Colgrove J,Dhai A,Gomez-Diaz R,Green SK,Kang G,Lagos R,Loh P,London AJ,Mulholland K,Neels P,Pitisuttithum P,Sarr SC,Selgelid M,Sheehan M,Smith PG

    更新日期:2014-08-20 00:00:00

  • Influenza vaccination in 22 developed countries: an update to 1995.

    abstract::This study expands and updates through 1995 our earlier report on influenza vaccine use in 18 developed countries. Five of the six countries with high levels of vaccine use in 1992 (> or = 130 doses/1000 population) showed little change or slight declines over the subsequent 3 years. The exception was the United State...


    pub_type: 杂志文章


    authors: Fedson DS,Hirota Y,Shin HK,Cambillard PE,Kiely J,Ambrosch F,Hannoun C,Leese J,Sprenger MJ,Hampson AW,Bro-Jørgensen K,Ahlbom AM,Nøkleby H,Valle M,Olafsson O,Salmerón F,Cloetta J,Rebelo de Andrade H,Snacken R,Donatell

    更新日期:1997-10-01 00:00:00

  • Immunological responses and protection against Mycobacterium bovis in calves vaccinated with a low dose of BCG.

    abstract::Groups of calves (20 per group) were vaccinated subcutaneously with a single dose of BCG Pasteur (6 x 10(4) or 6 x 10(6) colony forming units) and two months later, 15 calves from each group were challenged intratracheally with virulent Mycobacterium bovis. Vaccination with either dose of BCG induced significant prote...


    pub_type: 杂志文章


    authors: Buddle BM,de Lisle GW,Pfeffer A,Aldwell FE

    更新日期:1995-08-01 00:00:00

  • The cellular immune response to influenza vaccination is preserved in rheumatoid arthritis patients treated with rituximab.

    abstract:OBJECTIVES:Yearly vaccination against influenza is currently recommended to patients with rheumatoid Arthritis (RA). Antibody and cell-mediated responses are both involved in the defense against influenza. Humoral responses to influenza vaccine are impaired in RA patients treated with rituximab (RTX). The objectives of...


    pub_type: 杂志文章


    authors: Arad U,Tzadok S,Amir S,Mandelboim M,Mendelson E,Wigler I,Sarbagil-Maman H,Paran D,Caspi D,Elkayam O

    更新日期:2011-02-11 00:00:00

  • Expression of cytokine genes following pre- and post-hatch immunization of chickens with herpesvirus of turkeys.

    abstract::Induction of immune response as characterised by expression of cytokine genes in the spleen following immunization of pre- and post-hatch chickens with herpesvirus of turkeys (HVT) vaccine was studied. The pattern of expression of IFN-gamma and IL-10 genes in pre-hatch immunized chickens was different from that observ...


    pub_type: 杂志文章


    authors: Abdul-Careem MF,Hunter DB,Lambourne MD,Read LR,Parvizi P,Sharif S

    更新日期:2008-05-02 00:00:00

  • Influenza vaccine production for Brazil: a classic example of successful North-South bilateral technology transfer.

    abstract::Technology transfer is a promising approach to increase vaccine production at an affordable price in developing countries. In the case of influenza, it is imperative that developing countries acquire the technology to produce pandemic vaccines through the transfer of know-how, as this will be the only way for the majo...


    pub_type: 杂志文章,评审


    authors: Miyaki C,Meros M,Precioso AR,Raw I

    更新日期:2011-07-01 00:00:00

  • Immunogenicity and protective efficacy of novel Mycobacterium tuberculosis antigens.

    abstract::With tuberculosis continuing to be a major cause of global morbidity and mortality, a new vaccine is urgently needed. Tuberculosis subunit vaccines have been shown to induce robust immune responses in humans and are a potentially safer alternative to BCG for use in HIV-endemic areas. In this study, we investigated the...


    pub_type: 杂志文章


    authors: Derrick SC,Yabe IM,Yang A,Kolibab K,Hollingsworth B,Kurtz SL,Morris S

    更新日期:2013-09-23 00:00:00

  • Oral cholera vaccine delivery strategy in India: Routine or campaign?-A scoping review.

    abstract::Oral Cholera Vaccine (OCV) has been recognized as an adjunct tool for prevention and control of cholera. However, policy directions are currently unavailable in India to guide the vaccine delivery. We conducted a scoping review to inform the policy about the scopes and challenges of different strategic choices of OCV ...


    pub_type: 杂志文章,评审


    authors: Ray A,Sarkar K,Haldar P,Ghosh R

    更新日期:2020-02-29 00:00:00

  • Acceptability of intranasal live attenuated influenza vaccine, influenza knowledge and vaccine intent in The Gambia.

    abstract:BACKGROUND:The burden of influenza is increasingly recognised in Africa. The WHO recommends introducing influenza vaccination to high-risk groups: pregnant women, children <5 years, and the elderly. The Gambia currently has no influenza vaccination policy, but the NASIMMUNE study, a clinical trial of intranasal live at...


    pub_type: 杂志文章


    authors: Armitage EP,Camara J,Bah S,Forster AS,Clarke E,Kampmann B,de Silva TI

    更新日期:2018-03-20 00:00:00

  • Naturally occurring antibodies for the group B streptococcal surface immunogenic protein (Sip) in pregnant women and newborn babies.

    abstract::Sip is a surface-exposed protein of GBS, which causes severe neonatal disease. Because Sip elicits a protective immune response in mice, we assessed whether pregnant women and newborns have Sip antibodies. Sera were collected from 644 pregnant women and 176 of their healthy newborns, and 10 newborns with GBS disease a...


    pub_type: 杂志文章


    authors: Manning SD,Wood S,Kasha K,Martin D,Rioux S,Brodeur B,Davies HD

    更新日期:2006-11-17 00:00:00

  • Influenza vaccination of future healthcare workers: a cross-sectional study of uptake, knowledge and attitudes.

    abstract::Promotional campaigns recommend immunisation against influenza in healthcare workers (HCWs) but the uptake in this group remains low. We conducted a survey study during the 2008-2009 influenza vaccination period amongst future HCWs to quantify uptake and identify barriers to immunisation. Overall uptake was 8.0% (95% ...


    pub_type: 杂志文章


    authors: Blank DL,Bodansky DM,Forbes A,Garde E,Story F,Roalfe AK,Tait L

    更新日期:2010-06-23 00:00:00

  • Immunogenicity and protective efficacy of recombinant Haemophilus parasuis SH0165 putative outer membrane proteins.

    abstract::Haemophilus parasuis (H. parasuis), the causative agent of swine polyserositis, polyarthritis, and meningitis, is one of the most important bacterial diseases of pigs worldwide. Little vaccines currently exist that have a significant effect on infections with all pathogenic serovars of H. parasuis. H. parasuis putativ...


    pub_type: 杂志文章


    authors: Fu S,Zhang M,Xu J,Ou J,Wang Y,Liu H,Liu J,Chen H,Bei W

    更新日期:2013-01-02 00:00:00

  • Vaccines and the regulatory arm of the immune system. An overview from the Trypanosoma cruzi infection model.

    abstract::The knowledge that the immune system is composed of a regulatory/suppressor arm added a new point of view to better understand the nature of several pathologies including cancer, transplants, infections and autoimmune diseases. The striking discoveries concerning molecules and cells involved in this kind of regulation...


    pub_type: 杂志文章,评审


    authors: Cabrera G,Marcipar I

    更新日期:2019-06-19 00:00:00

  • Infants with low vaccine antibody responses have altered innate cytokine response.

    abstract::We recently identified a population of 10% of infants who respond with sub-protective antibody levels to most routine primary pediatric vaccinations due to altered innate and adaptive immune responses. We term these infants as low vaccine responders (LVRs). Here we report new data showing that TLR7/8 agonist - R848 st...


    pub_type: 杂志文章


    authors: Surendran N,Nicolosi T,Pichichero M

    更新日期:2016-11-11 00:00:00

  • Prevalence and characteristics of HPV vaccine hesitancy among parents of adolescents across the US.

    abstract:BACKGROUND:While many clinicians encounter parents or adolescents who refuse HPV vaccine, little is known about the prevalence of hesitancy for HPV vaccine nationally or its association with vaccination. METHODS:In April 2019, we surveyed families with adolescents 11-17 years using a national online panel (Knowledge P...


    pub_type: 杂志文章


    authors: Szilagyi PG,Albertin CS,Gurfinkel D,Saville AW,Vangala S,Rice JD,Helmkamp L,Zimet GD,Valderrama R,Breck A,Rand CM,Humiston SG,Kempe A

    更新日期:2020-08-27 00:00:00

  • A reduction in chronic hepatitis B virus infection prevalence among children in Vietnam demonstrates the importance of vaccination.

    abstract:BACKGROUND:Vietnam has high endemic hepatitis B virus infection with >8% of adults estimated to have chronic infection. Hepatitis B vaccine was first introduced in the national childhood immunization program in 1997 in high-risk areas, expanded nationwide in 2002, and included birth dose vaccination in 2003. This surve...


    pub_type: 杂志文章


    authors: Nguyen TH,Vu MH,Nguyen VC,Nguyen LH,Toda K,Nguyen TN,Dao S,Wannemuehler KA,Hennessey KA

    更新日期:2014-01-03 00:00:00

  • Tick-borne encephalitis--pathogenesis, clinical course and long-term follow-up.

    abstract::The prospective studies available today confirm the experience gained from several retrospective studies that TBE is a disease with a severe acute clinical course and considerable long-term morbidity. A defined post-encephalitic TBE syndrome exists, causing long-lasting morbidity that often affects the quality of life...


    pub_type: 杂志文章,评审


    authors: Haglund M,Günther G

    更新日期:2003-04-01 00:00:00

  • Dendritic cells stimulated with outer membrane protein A (OmpA) of Salmonella typhimurium generate effective anti-tumor immunity.

    abstract::Gram-negative bacterial outer membrane proteins (Omps) have an important role in pathogenesis and signal reception. We previously reported that Acinetobacter OmpA (AbOmpA) induced maturation of bone marrow-derived dendritic cells (BMDCs) and that AbOmpA-primed DCs produced IL-12 which generated Th1 CD4(+) T-cells. We ...


    pub_type: 杂志文章


    authors: Jang MJ,Kim JE,Chung YH,Lee WB,Shin YK,Lee JS,Kim D,Park YM

    更新日期:2011-03-16 00:00:00

  • An enriched medical home intervention using community health workers improves adherence to immunization schedules.

    abstract:IMPORTANCE:Disparities in childhood vaccination rates persist. OBJECTIVE:To evaluate the impact of an enriched medical home intervention using community health workers on improving immunization adherence among young children. DESIGN:The intervention group received home visits from trained community health workers to ...


    pub_type: 杂志文章


    authors: Pati S,Ladowski KL,Wong AT,Huang J,Yang J

    更新日期:2015-11-17 00:00:00

  • Construction and biological characterization of artificial recombinants between a wild type flavivirus (Kunjin) and a live chimeric flavivirus vaccine (ChimeriVax-JE).

    abstract::Although the theoretical concern of genetic recombination has been raised related to the use of live attenuated flavivirus vaccines [Seligman, Gould, Lancet 2004;363:2073-5], it has little foundation [e.g., Monath TP, Kanesa-Thasan N, Guirakhoo F, Pugachev K, Almond J, Lang J, et al. Vaccine 2005;23:2956-8]. To invest...


    pub_type: 杂志文章


    authors: Pugachev KV,Schwaiger J,Brown N,Zhang ZX,Catalan J,Mitchell FS,Ocran SW,Rumyantsev AA,Khromykh AA,Monath TP,Guirakhoo F

    更新日期:2007-09-17 00:00:00

  • Implementation of a Q fever vaccination program for high-risk patients in the Netherlands.

    abstract:BACKGROUND:Between 2007 and 2011 the Netherlands was faced with an unprecedented Q fever outbreak with more than 4000 people affected. Dairy goats were considered the main source of infection. In addition to taking veterinary measures, the Dutch government offered an unlicensed vaccine against the causative bacterium C...


    pub_type: 杂志文章


    authors: Isken LD,Kraaij-Dirkzwager M,Vermeer-de Bondt PE,Rümke HC,Wijkmans C,Opstelten W,Timen A

    更新日期:2013-05-28 00:00:00

  • Evaluation of safety and immunogenicity of recombinant influenza hemagglutinin (H5/Indonesia/05/2005) formulated with and without a stable oil-in-water emulsion containing glucopyranosyl-lipid A (SE+GLA) adjuvant.

    abstract:BACKGROUND:Expression of recombinant hemagglutinin (rHA) in insect cells represents a technology with proven efficacy in seasonal influenza and with the potential for a rapid response to the emergence of new, pandemic strains. We evaluated the safety and immunogenicity of rHA vaccine (H5/Indonesia/5/05) produced in SF+...


    pub_type: 杂志文章,随机对照试验


    authors: Treanor JJ,Essink B,Hull S,Reed S,Izikson R,Patriarca P,Goldenthal KL,Kohberger R,Dunkle LM

    更新日期:2013-11-19 00:00:00

  • Immunization with recombinant paraflagellar rod protein induces protective immunity against Trypanosoma cruzi infection.

    abstract::In the present study, we have produced recombinant paraflagellar rod proteins (PFR) and report their use for successful vaccination of mice against Trypanosoma cruzi. This protection is associated with a highly polarized type 1 cytokine production profile. Additionally, we have analyzed the gene sequence encoding PFR-...


    pub_type: 杂志文章


    authors: Luhrs KA,Fouts DL,Manning JE

    更新日期:2003-06-20 00:00:00

  • Effect of thiomersal on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles as assessed by novel ELISAs specific for either 146S or 12S particles.

    abstract::Intact (146S) foot-and-mouth disease virions (FMDVs) can dissociate into specific (12S) viral capsid degradation products. Using two single-domain antibody fragments that bind specifically to either 146S or 12S particles we developed two ELISAs for the quantification of these particles in FMDV antigen preparations use...


    pub_type: 杂志文章


    authors: Harmsen MM,Fijten HP,Westra DF,Coco-Martin JM

    更新日期:2011-03-24 00:00:00

  • Resilience of HPV vaccine uptake in Denmark: Decline and recovery.

    abstract:BACKGROUND:Immunization programs' resilience to shocks is central to their success, but little empirical evidence documents resilience in action. We sought to characterize the decline of HPV vaccination in Denmark after negative media coverage and recovery during a national information campaign. METHODS:We conducted a...


    pub_type: 杂志文章


    authors: Hansen PR,Schmidtblaicher M,Brewer NT

    更新日期:2020-02-11 00:00:00

  • Pandemic influenza H1N1 2009 infection in Victoria, Australia: no evidence for harm or benefit following receipt of seasonal influenza vaccine in 2009.

    abstract::Conflicting findings regarding the level of protection offered by seasonal influenza vaccination against pandemic influenza H1N1 have been reported. We performed a test-negative case control study using sentinel patients from general practices in Victoria to estimate seasonal influenza vaccine effectiveness against la...


    pub_type: 杂志文章


    authors: Kelly HA,Grant KA,Fielding JE,Carville KS,Looker CO,Tran T,Jacoby P

    更新日期:2011-08-26 00:00:00

  • Identification of population at risk for future Clostridium difficile infection following hospital discharge to be targeted for vaccine trials.

    abstract:BACKGROUND:Efforts to develop a Clostridium difficile vaccine are underway; identification of patients at risk for C. difficile infection (CDI) is critical to inform vaccine trials. We identified groups at high risk of CDI ≥ 2 8 days after hospital discharge. METHODS:Hospital discharge data and pharmacy data from two ...


    pub_type: 杂志文章


    authors: Baggs J,Yousey-Hindes K,Ashley ED,Meek J,Dumyati G,Cohen J,Wise ME,McDonald LC,Lessa FC

    更新日期:2015-11-17 00:00:00

  • Vaccination strategies for managing brucellosis in Yellowstone bison.

    abstract::Concerns over migratory bison (Bison bison) at Yellowstone National Park transmitting brucellosis (Brucella abortus) to cattle herds on adjacent lands led to proposals for bison vaccination. We developed an individual-based model to evaluate how brucellosis infection might respond under alternate vaccination strategie...


    pub_type: 杂志文章


    authors: Treanor JJ,Johnson JS,Wallen RL,Cilles S,Crowley PH,Cox JJ,Maehr DS,White PJ,Plumb GE

    更新日期:2010-10-01 00:00:00

  • No evidence for priming response in Galleria mellonella larvae exposed to toxin protein PirA2B2 from Photorhabdus luminescens TT01: An association with the inhibition of the host cellular immunity.

    abstract::There is accumulating evidence that many invertebrates including insects can acquire enhanced immune protection against subsequently pathogens infection through immune priming. However, whether the toxin protein from pathogenic bacteria can induce such priming response remains unclear. Here we cloned, expressed and pu...


    pub_type: 杂志文章


    authors: Wu G,Yi Y,Sun J,Li M,Qiu L

    更新日期:2015-11-17 00:00:00