Abstract：
:Dengue is a major infectious disease that affects people living in tropical and subtropical regions around the world. The causative agents are dengue virus serotype 1, 2, 3, and 4 (DENV1, 2, 3, and 4). Developing a vaccine for dengue is a high priority for public health, but traditional methods have faced numerous obstacles due to the unique immunopathogenesis of dengue virus infection. Here, we report a novel dengue vaccine candidate based on dengue pseudoinfectious virus (PIV) produced by the incorporation of a dengue subgenomic replicon into viral particles in highly efficient packaging cells. The subgenomic replicon was constructed by deleting the capsid protein (C) gene from the dengue viral genome and optimizing the signal peptide sequence of pre-membrane protein (prM) to facilitate the formation of viral particles. Packaging cells were developed for inducible expression of a bi-protein Cpr, where the protein pr is the "pr" segment of viral protein prM that holds the protein C on the endoplasmic reticulum (ER). When the replicon was introduced into the packaging cells, protein C was released from the bi-protein Cpr by a replicon-encoded viral protease. Coordinate expression of viral structural proteins by the replicon and packaging cells led to the incorporation of the replicon into viral particle to produce PIVs. Animal tests showed that the dengue PIV vaccine was highly immunogenic and the immune response protected mice challenged with a hundred-fold LD50 inoculation of dengue virus. The method described here has the potential to be applied to vaccine development for other flaviviruses.
journal_name
Vaccinejournal_title
Vaccineauthors
Pang X,Guo Y,Zhou Y,Fu W,Gu Xdoi
10.1016/j.vaccine.2014.03.091subject
Has Abstractpub_date
2014-06-24 00:00:00pages
3854-60issue
30eissn
0264-410Xissn
1873-2518pii
S0264-410X(14)00482-4journal_volume
32pub_type
杂志文章相关文献
VACCINE文献大全abstract：:Taking the results of a prospective cohort study by our group that evaluated the effectiveness of the inactivated subunit virosomal influenza vaccine (Inflexal V), Crucell-Berna) in the prevention of influenza-related diseases and the reduction of its negative economic consequences, the economic costs and benefits for...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.01.053
更新日期：2009-05-26 00:00:00
abstract：:Information provided by most influenza vaccine manufacturers do not reflect the recommendations of WHO and/or national public health advisory groups with regard to the use of influenza vaccines in pregnant or lactating women. The majority of vaccines contain precautionary language which could discourage use in pregnan...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2016.08.042
更新日期：2016-10-26 00:00:00
abstract：:Interleukin-5 (IL-5) is a cytokine which is essential for the maturation of eosinophils in bone marrow and for their release into the blood. Eotaxin is a CC type chemokine implicated in the recruitment of eosinophils in a variety of inflammatory disorders. Since eosinophil-activity is governed by these two pathways, w...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.02.048
更新日期：2010-04-19 00:00:00
abstract：BACKGROUND:Unbiased deep sequencing offers the potential for improved adventitious virus screening in vaccines and biotherapeutics. Successful implementation of such assays will require appropriate control materials to confirm assay performance and sensitivity. METHODS:A common reference material containing 25 target ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.12.020
更新日期：2016-04-12 00:00:00
abstract：:Non-pathogenic lactic acid bacteria (LAB) are attractive as live carriers to deliver protective antigens to the mucosal immune system. Both persisting and non-persisting strains of lactic acid bacteria have been evaluated and seem to work equally well by the systemic and nasal routes of administration. However, it is ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(02)00301-8
更新日期：2002-09-10 00:00:00
abstract：:Chlamydia trachomatis causes respiratory and sexually transmitted infections. Here, we tested a vaccine formulated with the recombinant major outer membrane protein from C. trachomatis mouse pneumonitis (CT-MoPn) for its ability to protect mice against an intranasal (i.n.) challenge. The adjuvants CpG and Montanide we...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.09.040
更新日期：2010-11-10 00:00:00
abstract：:Defective interfering (DI) virus RNAs result from major deletions in full-length viral RNAs that occur spontaneously during de novo RNA synthesis. These RNAs are packaged into virions that are by definition non-infectious, and are delivered to cells normally targeted by the virion. DI RNAs can only replicate with the ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2006.03.004
更新日期：2006-05-15 00:00:00
abstract：:Necrotic enteritis (NE) is a severe disease of chickens and turkeys caused by some strains of Clostridium perfringens type A. The disease is well controlled by the use of in-feed antibiotic growth promoters (AGPs). However, due to worldwide public and regulatory pressure to reduce the use of AGPs inter alia, there is ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2017.10.030
更新日期：2017-12-14 00:00:00
abstract：INTRODUCTION:Pan American Health Organization's (PAHO) ProVac Initiative aims to strengthen countries' technical capacity to make evidence-based immunization policy. With financial support from the Bill and Melinda Gates Foundation, PAHO established the ProVac International Working Group (IWG), a platform created for t...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.10.090
更新日期：2015-05-07 00:00:00
abstract：:Therapeutic immunization of HIV-1-infected individuals with or without anti-retroviral therapy is a new promising disease prevention. To induce a new cytotoxic T(CD8) lymphocyte (CTL) immunity during chronic HIV-1 infection 15 infrequently targeted but conserved HLA-supertype binding CTL epitopes from Gag, Pol, Nef, E...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.07.025
更新日期：2011-09-16 00:00:00
abstract：:Vaccination of health-care workers (HCWs) against seasonal influenza has been consistently recommended worldwide in order to prevent nosocomial transmission and ensure delivery of health-care services during outbreaks. We describe the effects of a nationwide campaign to promote influenza vaccination among HCWs working...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.06.108
更新日期：2010-08-23 00:00:00
abstract：:Plasmodium vivax is responsible for an approximate 35 million yearly human cases of malaria. Unfortunately, due to the low mortality rate associated with it and the difficulties of continuously in vitro culturing of this parasite, vaccine development against this human malaria has been largely neglected. In here, the ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(99)00176-0
更新日期：1999-08-06 00:00:00
abstract：BACKGROUND:Several chronic disease states have been identified as pneumococcal vaccination indications due to their ability to increase pneumococcal disease development and subsequent mortality. However, the risk of mortality according to the number of these disease states present is unknown. We sought to determine the...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2017.02.026
更新日期：2017-03-23 00:00:00
abstract：:The Decade of Vaccines (DoV) initiative, launched in 2010, has as its mission "to extend, by 2020 and beyond, the full benefits of immunization to all people, regardless of where they are born, who they are, or where they live". Through their life-saving vaccines, the research-based vaccine companies represented by th...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2012.12.032
更新日期：2013-04-18 00:00:00
abstract：:A cross-sectional study was carried out among HCPs in Northwest General Hospital & Research Centre, Hayatabad Peshawar, Pakistan. The purpose of this study was to investigate knowledge, awareness and attitude of HCPs towards influenza vaccination. A total of N=170 questionnaires were distributed among the staff. There...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2016.01.045
更新日期：2016-03-08 00:00:00
abstract：:Intranasal (i.n.) vaccination with two suboptimal doses of 8 microg of deglycosylated chain A ricin (DGCA) stimulated low anti-ricin ELISA IgG and neutralizing antibody responses and the vaccine was only marginally protective against a lethal ricin toxin aerosol challenge. However, in the presence of 4, 2, or 1 microg...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.12.034
更新日期：2006-03-15 00:00:00
abstract：:Immunization with amyloid-beta (Abeta) peptide reduces amyloid load in animal studies and in humans; however clinical trials resulted in the development of a pro-inflammatory cellular response to Abeta. Apoptosis has been employed to stimulate humoral and Th2-biased cellular immune responses. Thus, we sought to invest...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.12.044
更新日期：2009-02-25 00:00:00
abstract：:In the present study we assessed the capacity of recombinant E. coli- or plasmid-expressed chicken interferons (IFN) and chicken IL-1beta, to exert immunostimulatory activities for humoral immune responses, in day-old and adult chickens. We observed that both recombinant E. coli-expressed chicken IFN-alpha/beta and IF...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(99)00537-x
更新日期：2000-04-14 00:00:00
abstract：:Aging is accompanied by a decline in immune function which can lead to decreased responses to vaccines. Attenuated recombinant Vibrio cholerae O1 vaccine strain CVD 103-HgR elicits a rapid serum vibriocidal antibody (SVA) response and protects against cholera diarrhea in volunteer challenge studies but has not been st...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2019.01.077
更新日期：2019-03-07 00:00:00
abstract：:Coli surface antigen 6, CS6, is one of the most prevalent colonization factors (CFs) associated with Enterotoxigenic Escherichia coli (ETEC) bacteria, the most common cause of diarrhea among infants and children in developing countries. The CS6 operon encodes two structural subunit proteins, CssA and CssB, a chaperon,...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.07.091
更新日期：2008-10-03 00:00:00
abstract：:Vector-directed lymphokine expression represents a novel approach to the attenuation of live recombinant viruses which might be used as vaccines. Expression of interleukin-2 (IL-2) by recombinant vaccinia virus has been shown to significantly attenuate virus virulence in rodent species without diminishing immunogenici...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(90)90171-h
更新日期：1990-02-01 00:00:00
abstract：INTRODUCTION:Observational studies of influenza vaccine effectiveness often study persons seeking medical care for acute respiratory infection (ARI). We conducted a pilot study to determine if vaccine effectiveness could be estimated in the general population with a novel rolling cross-sectional survey sampling design ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.09.051
更新日期：2014-11-12 00:00:00
abstract：INTRODUCTION:Immunization education for physicians-in-training is crucial to address vaccine concerns in clinical practice. Vaccine education is not standardized across residency programs. The Collaboration for Vaccination Education and Research (CoVER) team developed an online curriculum for pediatric (Peds) and famil...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.09.043
更新日期：2020-10-27 00:00:00
abstract：:An experimental infection with classical swine fever (CSF) virus in E2 sub-unit marker vaccine vaccinated gilts was conducted in order to evaluate the effect of vaccination on virus transmission and course of the disease. Therefore, clinical signs as well as horizontal and vertical virus transmission were monitored in...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(01)00320-6
更新日期：2001-10-12 00:00:00
abstract：:Actinobacillus pleuropneumoniae is a gram negative pleiomorphic rod that is the causative agent of a severe, highly infectious and often fatal pleuropneumonia in swine. We have previously reported the construction of genetically-defined stable riboflavin auxotrophs by replacement of a portion of the APP riboflavin bio...
journal_title：Vaccine
pub_type： 临床试验,杂志文章
doi：10.1016/s0264-410x(00)00076-1
更新日期：2000-06-15 00:00:00
abstract：:The emergence of pneumococcal strains resistant to penicillin caused a lot of problems in the therapy of invasive diseases, and added new dimensions to the role of immunisation. In addition to the currently available 23-valent pneumococcal polysaccharide vaccine (PPV) and a new 7-valent conjugate vaccine (PCV) (Prevna...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(03)00518-8
更新日期：2003-12-01 00:00:00
abstract：:Hepatitis B virus (HBV) infects millions of people worldwide and is a leading cause of liver cirrhosis and hepatocellular carcinoma. Current therapies based on nucleos(t)ide analogs or pegylated-interferon-α lead to control of viral replication in most patients but rarely achieve cure. A potential strategy to control ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2015.07.020
更新日期：2015-08-26 00:00:00
abstract：AIM:To investigate parents' views regarding potential new vaccines. We examined attitudes towards severity of specific infections, acceptability of potential vaccines and preferences for the number of injections they would want their child to receive on any one occasion. DESIGN:Cross sectional survey. METHODS:Parents...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.08.057
更新日期：2007-11-07 00:00:00
abstract：:A novel triterpenoidal saponin, called pulcherrimasaponin (CP05), isolated from the leaves of Calliandra pulcherrima Benth. shows remarkable similarities to the previously described potent adjuvant, QS21 saponin (Quillaja saponaria Molina). On the basis of chemical and physicochemical evidence, its structure was estab...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.08.022
更新日期：2005-01-11 00:00:00
abstract：:In July 1999, the Department of Health launched a campaign to immunise all children aged from 0-17 years with a new vaccine giving protection against meningococcal group C infection. Following the campaign, a survey of Immunisation Co-ordinators in England was conducted to identify strengths and weaknesses of the camp...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(03)00160-9
更新日期：2003-06-20 00:00:00
