Abstract:
:Immunostimulating complexes (ISCOMs) are subunit vaccines that are particularly effective in producing immunity against systemic viral infections, but their effectiveness against mucosal infections has received little attention. To study their ability to produce mucosal immune responses in the female reproductive tract, a model ISCOM was prepared containing sheep erythrocyte membrane proteins, and anti-erythrocyte IgA and IgG titres in mouse vaginal washings were measured after immunization at parenteral or local mucosal sites. The ISCOM was prepared by a modified procedure that resulted in incorporation of 10-15% of initial membrane protein compared with 1-5% previously reported. Electrophoretic analysis demonstrated that four out of five erythrocyte membrane proteins were incorporated into the ISCOM, and electron microscopic observations indicated that the ISCOM had a cage-like structure with a diameter of 40 nm, similar to previous ISCOMs. Immunization in the pelvic presacral space (p.s.-p.s.) stimulated significantly higher anti-erythrocyte IgA titres in vaginal fluid than were produced by intraperitoneal (i.p.-i.p.), subcutaneous (s.c.-s.c.), intravaginal (i.vag.-i.vag.), or i.p.-i.vag. immunizations with the same vaccine. Specific IgG titres were less dependent on the route of immunization, with p.s.-p.s., i.p.-i.p. and s.c.-s.c. administration all giving similar high titres while i.p.-i.vag. treatment induced lower titres. These observations using a model ISCOM indicate that mucosal immune responses against membrane proteins were elicited in the female reproductive tract, and that non-mucosal immunization in the pelvis was a more effective route of administration than local application of the ISCOM to the vaginal mucosa.
journal_name
Vaccinejournal_title
Vaccineauthors
Thapar MA,Parr EL,Bozzola JJ,Parr MBdoi
10.1016/0264-410x(91)90269-csubject
Has Abstractpub_date
1991-02-01 00:00:00pages
129-33issue
2eissn
0264-410Xissn
1873-2518pii
0264-410X(91)90269-Cjournal_volume
9pub_type
杂志文章相关文献
VACCINE文献大全abstract::The present study was undertaken to determine the effectiveness in including VP2 gene of the large segment genome of infectious bursal disease virus (IBDV) in the DNA vaccine for protection of chickens against infectious bursal disease (IBD). Different fragments of the large segment gene of IBDV standard challenge (ST...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00473-5
更新日期:2003-01-17 00:00:00
abstract:BACKGROUND:Challenges in reaching good vaccination coverage against measles emerged in several European Union/European Economic Area Member States (EU/EEA MS) leading to progressive accumulation of susceptible individuals and outbreaks. The Burden of Communicable Diseases in Europe (BCoDE) project developed a methodolo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.01.094
更新日期:2014-04-01 00:00:00
abstract::Previous work by our laboratory identified a fraction of Schistosoma mansoni soluble adult worm antigenic preparation, designated PIII, able to elicit significant in vitro cell proliferation, and lower in vitro and in vivo granuloma formation. In the present work, we investigated some biological activities of P24, an ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(00)00265-6
更新日期:2000-12-08 00:00:00
abstract::Phage lambda particles displaying four immunodominant regions of porcine Circovirus 2 (PCV2) capsid protein (LDP-D-CAP) was shown to be immunogenic in pigs. The immunodominant regions were fused to the carboxyl-terminal of lambda head protein D. Expression of D-CAP on lambda display particles was demonstrated by ELISA...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.08.019
更新日期:2009-11-05 00:00:00
abstract::This study aimed to evaluate the durability of the immunogenicity of MVA85A beyond infancy. Participants in an immunogenicity study of MVA85A administered at age of 4 months had additional evaluation 14 months after initial vaccination for IFN-γ ELISPOT responses to Ag85A peptide and ESAT6/CFP-10 and tuberculin skin t...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2012.06.054
更新日期:2012-08-17 00:00:00
abstract::Seasonal influenza vaccines represent a positive intervention to limit the spread of the virus and protect public health. Yet continual influenza evolution and its ability to evade immunity pose a constant threat. For these reasons, vaccines with improved potency and breadth of protection remain an important need. We ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.08.030
更新日期:2019-09-30 00:00:00
abstract::Rotavirus is acknowledged to be a major cause of acute gastroenteritis in infants and young children. As gastroenteritis due to rotavirus is a public health problem and two new vaccines are currently available, we investigated the rotavirus burden and developed a cost-effectiveness analysis, using data collected in th...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.01.054
更新日期:2009-05-26 00:00:00
abstract:PURPOSE:GEN-003 is a candidate therapeutic HSV-2 vaccine containing a fragment of infected cell protein 4 (ICP4.2), a deletion mutant of glycoprotein D2 (gD2ΔTMR), and Matrix-M2 adjuvant. In a dose-ranging phase 1/2a clinical trial, immunization with GEN-003 reduced viral shedding and the percentage of reported herpeti...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2016.09.001
更新日期:2016-10-17 00:00:00
abstract::Currently, two intradermal (ID) regimens for rabies post-exposure prophylaxis (PEP) are recommended by WHO and used in countries where approved by national authorities: the Thai Red Cross (TRC) two-site ID regimen and the eight-site ID regimen. Besides these WHO recommended schedules, a new economical four-site ID reg...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.vaccine.2006.02.036
更新日期:2006-05-08 00:00:00
abstract::HIV vaccines of the first generation are, in contrast to most currently licensed vaccines, unlikely to provide sterilizing immunity against infection with HIV. However, they are expected to exert a beneficial effect on the maintenance of CD4 T-cell counts by reducing viral load. There is a recognized need for clarifyi...
journal_title:Vaccine
pub_type:
doi:10.1016/j.vaccine.2008.12.029
更新日期:2009-03-23 00:00:00
abstract::The measurement of measles-specific neutralizing antibodies, directed against the surface measles virus hemagglutinin and fusion proteins, is considered the gold standard in measles serology. We assessed functional measles-specific neutralizing antibody levels in a racially diverse cohort of 763 young healthy adolesce...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.04.037
更新日期:2011-06-15 00:00:00
abstract::To identify predictive factors of complete and age-appropriate vaccination status in Greece, we conducted a cross-sectional study, using stratified cluster sampling, among children attending the first year of the Greek Grammar school (about 6 years of age) and their parents/guardians. Almost 88% (N=3878) of pupils in ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.11.078
更新日期:2010-02-17 00:00:00
abstract:PURPOSE:We conducted a cancer vaccine clinical trial with MAGE-A4 protein. Safety, clinical response, and antigen-specific immune responses were analyzed and the prognostic factors by vaccination were investigated. EXPERIMENTAL DESIGN:Twenty patients with advanced esophageal, stomach or lung cancer were administered M...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.09.002
更新日期:2014-10-14 00:00:00
abstract::Flagellin of Pseudomonas aeruginosa is an important vaccine candidate. N-terminal domains are highly conserved in both type a and type b flagellins. The efficacy of gold nanoparticles (AuNPs) conjugated to N-terminal domains of P. aeruginosa flagellin (flagellin(1-161)), as an immunogen in mice, has been assessed. The...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.01.041
更新日期:2016-03-14 00:00:00
abstract::A total of 105 hybridomas secreting anti-Japanese encephalitis (JE) virus monoclonal antibodies (mAbs) were generated from six fusions against four strains of JE virus: wild-type strains SA14 and G8924 and live attenuated vaccines SA14-5-3 and SA14-14-2 (PDK-9). Most of the mAbs (87%) elicited haemagglutination inhibi...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(92)90372-q
更新日期:1992-01-01 00:00:00
abstract::To evaluate long-term immunogenicity of a virosomal subunit inactivated influenza vaccine in children with asthma, a prospective study was conducted during 2005-2006 influenza season in six public pediatric clinics in Milan and surroundings, Northern Italy. A single dose (0.5 ml) of a virosomal subunit inactivated inf...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究
doi:10.1016/j.vaccine.2007.07.013
更新日期:2007-09-17 00:00:00
abstract::This commentary is a response to a systematic review, recently published in Vaccine, which investigates the effectiveness of educational interventions in increasing uptake of the human papillomavirus vaccine. The systematic review by Fu et al. (2014) enhances the field's understanding of human papillomavirus vaccine i...
journal_title:Vaccine
pub_type: 评论,杂志文章
doi:10.1016/j.vaccine.2014.09.045
更新日期:2014-11-12 00:00:00
abstract::Clinical and experimental studies of HIV-1 subcomponents were made in order to increase their immunogenicity. HIV subtype envelopes A, B and C have been compared and a detailed analysis made by peptides of the coreceptor-ligand interactions. We identified a direct interaction between HIV-1 envelope and a cellular rece...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/s0264-410x(02)00084-1
更新日期:2002-05-06 00:00:00
abstract::Putative TB vaccine candidates were selected from lists of genes induced in response to in vivo-like stimuli, such as low oxygen and carbon starvation or growth in macrophages, and tested as plasmid DNA vaccines for their ability to protect against Mycobacterium tuberculosis challenge in a guinea pig aerosol infection...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.05.025
更新日期:2006-09-11 00:00:00
abstract:BACKGROUND:Since special efforts are necessary to vaccinate people living far from fixed vaccination posts, decision makers are interested in knowing the economic value of such efforts. METHODS:Using our immunization geospatial information system platform and a measles compartment model, we quantified the health and e...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.03.007
更新日期:2019-04-17 00:00:00
abstract:BACKGROUND:Vaccine coverage estimates lag by years in the US. Commercially available medical claims databases contain timely records of childhood vaccinations given in physician offices. We used such data to track the replacement of the 7-valent pneumococcal conjugate vaccine (PCV7) by PCV13, a new vaccine active again...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.10.038
更新日期:2013-12-05 00:00:00
abstract::In the present study, cross-protection to bovine herpesvirus type 5 (BHV-5) induced by bovine herpesvirus type 1 (BHV-1) vaccination was examined following inoculation of rabbits and calves with a glycoprotein E (gE)-negative BHV-1 vaccine and subsequent challenge with BHV-5. Rabbits (n=5) and calves (n=8) were vaccin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.01.024
更新日期:2006-04-12 00:00:00
abstract::DNA vaccination is a promising alternative strategy for developing new human and animal vaccines. The massive efforts made these past 25 years to increase the immunizing potential of this kind of vaccine are still ongoing. A relatively small number of studies concerning poultry have been published. Even though there i...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2015.11.043
更新日期:2016-01-04 00:00:00
abstract::It is of paramount importance to know the vaccination status in internationally adopted children, so that they can be correctly immunized. This study ascertains the seroprotection rate for vaccine-preventable diseases and the validity of the immunization cards in 637 adopted children. The absence of the immunization c...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.08.029
更新日期:2008-10-29 00:00:00
abstract:INTRODUCTION:Pan American Health Organization's (PAHO) ProVac Initiative aims to strengthen countries' technical capacity to make evidence-based immunization policy. With financial support from the Bill and Melinda Gates Foundation, PAHO established the ProVac International Working Group (IWG), a platform created for t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.10.090
更新日期:2015-05-07 00:00:00
abstract::A formalin-inactivated red sea bream iridovirus (RSIV) vaccine was prepared using the culture supernatant of a persistently infected Pagrus major fin cell line (PI-PMF) with IVS-1 strain (RSIV subtype II Meglaocytivirus). Rock bream (Oplegnathus fasciatus) were injected with a high-dose, ultracentrifuged megalocytivir...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.11.001
更新日期:2020-12-03 00:00:00
abstract::A human influenza B/Lee/40 virus was cold-adapted by serial passages in embryonated chicken eggs, at progressively lower temperatures, for possible use as a future influenza B vaccine donor strain. Temperature sensitive and cold-adapted phenotypes were achieved as a consequence of the adaptation process. It was determ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.12.022
更新日期:2008-02-13 00:00:00
abstract::The immunogenicity of 23-valent pneumococcal polysaccharide vaccine was assessed in 57 HIV-1 infected former intravenous drug users and in 20 HIV-1 negative controls. The effect of vaccination on HIV-1 infection was studied in a subgroup of 38 patients, 60% of whom under highly active antiretroviral therapy (HAART). A...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00357-2
更新日期:2002-11-01 00:00:00
abstract::In accordance with the end game strategies for polio eradication a synchronized switch plan from tOPV to bOPV was implemented globally in 2016. The National Committee for Polio Eradication (NCCPE) validated the switch activities in India. An expert group of 104 academics conducted field visits in 25 states and 2 Union...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.02.038
更新日期:2019-04-17 00:00:00
abstract:BACKGROUND:BCG vaccine, introduced almost 100years ago, is the only option to prevent TB disease. It effectively protects newborns from meningeal TB but fails to prevent adult pulmonary TB. TB kills 1.3million people annually in areas where BCG vaccination is widely practiced. Thus, more effective TB vaccines are urgen...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.02.065
更新日期:2017-04-11 00:00:00