Abstract:
:The present study was undertaken to determine the effectiveness in including VP2 gene of the large segment genome of infectious bursal disease virus (IBDV) in the DNA vaccine for protection of chickens against infectious bursal disease (IBD). Different fragments of the large segment gene of IBDV standard challenge (STC) strain were successfully amplified by reverse transcription-polymerase chain reaction (RT-PCR) followed by cloning into a eukaryotic expression plasmid vector (pCR3.1) as DNA vaccines. Transient expression of the encoded genes from various constructed plasmids was characterized in COS-7 cells by positive immunofluorescent staining with polyclonal or monoclonal antibody to IBDV. Chickens (1-day old) were intramuscularly injected with the individual plasmid three times at weekly intervals, challenged with IBDV strain STC at 21-day old, and observed for 10 days. Chickens receiving the plasmids containing VP2 genes, including VP243, VP24, or VP2 fragment, did not have clinical signs, mortality, and bursal atrophy and were effectively protected against IBDV infection. On the contrary, chickens receiving plasmids without containing VP2 genes, including VP4, VP3, or VP43 fragment, had marked bursal atrophy and were not protected against IBD. Antigen detection was correlated with protection; chickens protected from IBDV infection had undetectable IBDV antigen in bursae. Enzyme-linked immunosorbent assay (ELISA) antibody titer to IBDV was low or undetectable prior to or after challenge with IBDV in protected chickens receiving the plasmids containing the VP2 gene. The results indicate that inclusion of VP2 gene in the plasmid DNA is essential in achieving effective protection mediated by DNA vaccination against IBDV infection in chickens.
journal_name
Vaccinejournal_title
Vaccineauthors
Chang HC,Lin TL,Wu CCdoi
10.1016/s0264-410x(02)00473-5subject
Has Abstractpub_date
2003-01-17 00:00:00pages
507-13issue
5-6eissn
0264-410Xissn
1873-2518pii
S0264410X02004735journal_volume
21pub_type
杂志文章相关文献
VACCINE文献大全abstract::Peripheral blood mononuclear cells were taken from subjects before and after oral immunization with cholera toxin B-subunit. Cells obtained from naive volunteers before immunization did not proliferate in vitro to B-subunit. Oral immunization induced a proliferative response in all volunteers with a peak stimulation i...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/0264-410x(93)90005-i
更新日期:1993-01-01 00:00:00
abstract::Recombinant plasmid DNA constructs expressing truncated or full-length dengue-1 envelope (E) with or without the pre-membrane (prM) were tested for immunogenicity in mice, as candidate dengue DNA vaccines. Two plasmids, one expressing the N-terminal 80% E and the other expressing prM and full length E were immunogenic...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00570-8
更新日期:2000-05-08 00:00:00
abstract::The level of protection conferred by foot-and-mouth disease (FMD) vaccines in primovaccinated animals primarily depends on the potency of the vaccine and the relatedness of the vaccine strain and circulating field isolate. The "Gold Standard" FMD vaccine potency test is the in vivo test performed in the target species...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.04.034
更新日期:2008-06-25 00:00:00
abstract::Vaccination is the principal means used to control and treat porcine reproductive and respiratory syndrome virus (PRRSV) infection. An array of PRRS vaccine products is available in various regions of the world. However, despite extensive efforts, little progress has been made to improve efficacy since the first intro...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2011.09.013
更新日期:2011-10-26 00:00:00
abstract::Vaccine induced responses are often weaker in those individuals most susceptible to infection, namely the very young and the elderly, highlighting the need for safe and effective vaccine adjuvants. Herein we evaluated different archaeosome formulations as an adjuvant to the H1N1 influenza hemagglutinin protein and com...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.07.010
更新日期:2019-11-08 00:00:00
abstract::The hepatitis B virus (HBV) has infected more than 2000 million persons alive today and 350 million persons are chronically infected carriers of the virus, at high risk of death from active hepatitis, cirrhosis and primary hepatocellular cancer. Each year approximately 1 million people die from the acute and chronic s...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(95)80050-n
更新日期:1995-01-01 00:00:00
abstract::Rhodococcus equi causes severe pneumonia in foals and has recently gained attention as a significant opportunistic pathogen in immunocompromised humans. However, no effective vaccine to prevent rhodococcosis is currently available. In this study, we have engineered the food-grade bacterium Lactococcus lactis to secret...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.10.026
更新日期:2011-12-09 00:00:00
abstract::The distribution of alpha-casein, bovine serum albumin (BSA), myoglobin and recombinant protective antigen (rPA) in mono-valent and combination vaccines containing aluminum hydroxide adjuvant was studied by fluorescence microscopy and flow cytometry. Green and red fluorescent probes were conjugated to the antigens. Ad...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.10.040
更新日期:2004-05-07 00:00:00
abstract::Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Arabian Peninsula that affects sheep, cattle, goats, camels, and humans. Effective vaccination of susceptible ruminants is important for the prevention of RVF outbreaks. Live-attenuated RVF vaccines are in general highly immunogenic...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.10.036
更新日期:2017-12-04 00:00:00
abstract::Pneumococcal type 1 pilus proteins have been proposed as potential vaccine candidates. Following conjugate pneumococcal vaccination, the prevalence of the pneumococcal type 1 pilus declined dramatically, a decline associated with the elimination of vaccine-type (VT) strains. Here we show that between 2004 and 2007, th...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.04.042
更新日期:2010-07-05 00:00:00
abstract:BACKGROUND:Adverse events following immunization (AEFI) requires special consideration in patients with primary immunodeficiency diseases (PID) because they may represent a "red flag" for the initial diagnosis and may cause disease complications. Therefore, the definition of appropriate vaccination schemes is a major i...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.01.047
更新日期:2016-03-18 00:00:00
abstract::In an effort to develop a safe and effective vaccine for the prevention of enterotoxigenic Escherichia coli (ETEC) K99 infections, we have developed a surface antigen display system using pgsA (poly-gamma-glutamate synthetase A) as an anchoring matrix. The recombinant fusion proteins comprised of pgsA and fimbriae pro...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.05.088
更新日期:2010-05-28 00:00:00
abstract::A mass vaccination campaign against influenza A/H1N1 was launched in France in October 2009. Vaccination was offered free of charge to the entire population according to a pre-defined order of priority. Demographic data and data on vaccinations given were recorded in a dedicated database. We analysed vaccine uptake by...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.09.096
更新日期:2010-11-29 00:00:00
abstract::Dengue fever is a growing public health concern around the world and despite vaccine development efforts, there are currently no effective dengue vaccines. In the present study we report the induction of protective antibodies against dengue virus by DNA immunization with domain III (DIII) region of the envelope protei...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.12.028
更新日期:2005-05-16 00:00:00
abstract::The development of live attenuated vaccines, allowing for the safe and effective immunisation at mucosal surfaces, is a strategy of great interest for vaccinologists. The main advantage of this approach over conventional parenteral vaccines is the induction of strong mucosal immune responses, allowing targeting of the...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2003.12.033
更新日期:2004-06-23 00:00:00
abstract::Rabies virus remains an important burden of disease claiming an estimated 60,000 lives each year, mainly children, and having a huge economical and societal cost. Post-exposure prophylaxis (PEP) is highly effective, however in patients that present with neurological symptoms the case-fatality ratio is extremely high (...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2017.12.051
更新日期:2019-08-02 00:00:00
abstract::Synthetic oligonucleotides (ODNs) containing immunostimulatory CpG motifs (CpG) are a new class of adjuvants suitable for the development of recombinant vaccines. Here we describe that endogenous interferon (IFN) was critical for the adjuvant activity of CpG ODN as genetically deficient mice developed significantly lo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.05.031
更新日期:2007-08-10 00:00:00
abstract::This study assessed (1) T-cell responses following vaccination of HIV-1 negative volunteers with HIV-1LAI recombinant gp160 (rgp160) vaccine, and (2) effects of vaccine injection schedule and MHC HLA type on those responses. In one trial, volunteers received rgp160 in two accelerated schedules (Groups 1 and 2). In the...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0264-410x(95)00020-2
更新日期:1995-09-01 00:00:00
abstract::ErbB-2 is associated with several solid tumours of which breast cancer is the commonest cancer in women worldwide. Though anti-ErbB-2 antibody appears to play a significant role in prevention and therapy, naturally occurring anti-ErbB-2 antibody associated with the cleaved ectodomain of overexpressed ErbB-2 self antig...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.10.077
更新日期:2014-02-12 00:00:00
abstract::Vesicular stomatitis virus (VSV) has been extensively utilized as a viral vector system for the induction of protective immune responses against a variety of pathogens. We constructed recombinant VSVs specifying either the Indiana or Chandipura virus G glycoprotein and expressing the West Nile virus (WNV) envelope (E)...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.11.087
更新日期:2009-02-05 00:00:00
abstract::Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a potent threat to human health. Although immunization with recombinant poxviruses expressing protective antigens as vaccines has been widely used for against many infectious diseases, development of recombinant swinepox virus (...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.02.028
更新日期:2012-04-26 00:00:00
abstract:BACKGROUND:One of the most concerning public health issues, related to vaccination and disease prevention, is the inability to induce durable immune responses following a single-dose immunization. In this regard, the nature of the inflammatory environment induced by vaccine adjuvants can negatively impact the resulting...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.04.004
更新日期:2019-05-06 00:00:00
abstract:BACKGROUND:We assessed 2 investigational 11- and 12-valent vaccines, containing capsular polysaccharides of 10 serotypes as in the pneumococcal non-typeable Haemophilus influenzae protein D-conjugate vaccine (PHiD-CV) and CRM197-conjugated capsular polysaccharides of serotypes 19A (11-valent) or 19A and 6A (12-valent)....
journal_title:Vaccine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.vaccine.2018.07.023
更新日期:2019-01-03 00:00:00
abstract::Clinical leprosy is characterized by varying manifestations between tuberculoid and lepromatous leprosy. In the former state the patient is able to elicit a cell-mediated immune response whereas in the latter, there is usually a humoral response. An understanding of this immunological balance is crucial in the search ...
journal_title:Vaccine
pub_type: 历史文章,杂志文章,评审
doi:10.1016/0264-410x(84)90037-9
更新日期:1984-12-01 00:00:00
abstract::An economic evaluation of reducing the age threshold for routine influenza vaccination in Spain from 65 to 50 years was performed. A probabilistic model was used to compare a policy based on a recommendation to vaccinate all adults aged 50-64 with the existing vaccination policy for that age group, during interpandemi...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.07.033
更新日期:2007-09-28 00:00:00
abstract::Two double-blind randomised controlled studies (phase I and I/II) were performed to assess for the first time the safety and immunogenicity of a recombinant subunit gp350 Epstein-Barr virus (EBV) vaccine in 148 healthy adult volunteers. All candidate vaccine formulations had a good safety profile and were well tolerat...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2007.04.008
更新日期:2007-06-11 00:00:00
abstract:OBJECTIVES:We aimed to estimate vaccine effectiveness against severe measles based on the number of vaccine doses administered and the time since last vaccination. PATIENTS AND METHODS:We included measles cases aged at least 2 years and born since 1980 who were notified in France between 2006 and mid-2019. We consider...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.05.005
更新日期:2020-06-15 00:00:00
abstract::Since the refinement of tissue culture techniques for virus isolation and propagation from the mid 1960s onwards, veterinary virology has received much academic and industrial interest, and has now become a major global industry largely centred on vaccine development against economically important virus diseases of fo...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2008.12.063
更新日期:2009-03-13 00:00:00
abstract:BACKGROUND:The recent Ebola outbreak in West Africa led to the use of a variety of different platform technologies for assaying antibodies because of the difficulties of handling the live virus. The same types of method could be applied rapidly to other infections when they emerge. There is a need to compare quantitati...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.11.083
更新日期:2017-03-01 00:00:00
abstract::To evaluate the safety and immunogenicity of a diphtheria, tetanus, acellular pertussis and Haemophilus infuenzae Type b (DTaP/Hib) combination vaccine first developed by a Chinese manufacturer, a randomized, two-stage, parallel controlled, single center clinical trial was conducted in Dafeng, Jiangsu Province of Chin...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2010.03.061
更新日期:2010-06-07 00:00:00