Abstract:
:A formalin-inactivated red sea bream iridovirus (RSIV) vaccine was prepared using the culture supernatant of a persistently infected Pagrus major fin cell line (PI-PMF) with IVS-1 strain (RSIV subtype II Meglaocytivirus). Rock bream (Oplegnathus fasciatus) were injected with a high-dose, ultracentrifuged megalocytivirus vaccine (Ultra HSCMV, 7.0 × 1010 copies/mL), a high-dose supernatant of cultured megalocytivirus vaccine (HSCMV, 1.0 × 1010 copies/mL), a supernatant of cultured megalocytivirus vaccine (SCMV, 1.0 × 109 copies/mL), and a low-dose of cultured megalocytivirus vaccine (LSCMV, 1.0 × 108 copies/mL). The vaccine efficacies for the various vaccine formulations were determined done following injection challenge with IVS-1 (1.0 × 104 copies/0.1 mL/fish), and the four different vaccines exhibited cumulative mortalities of 10.0 ± 0.0%, 48.3 ± 7.6%, 75.0 ± 5.0%, and 100.0 ± 0.0%, respectively. Additionally, the dose-dependent vaccine efficacy was also confirmed using two different cohabitation methods that included challenges G (general) and I (individual). When squalene + aluminum hydroxide (SqAl) was used as an adjuvant for the HSCMV or SCMV vaccine, cumulative mortalities of 30.0 ± 5.0% and 48.3 ± 7.6%, respectively, were obtained; moreover, these two adjuvants exhibited the highest efficacy in this study. The observed difference in survival post-challenge for the different vaccine concentrations was not reflected in the differences in neutralizing antibody titers. It was found that the water temperature during immune induction plays a less important a role than the water temperature during the challenge test, in which lowering the water temperature from 25 °C to 21 °C during a challenge improved the level of protection from cumulative mortalities from 35% to 10%. This study demonstrated that protection against mortality using inactivated vaccines against RSIVD in rock bream, which are known to be the most susceptible species to RSIV infection, is dependent upon antigen dose and temperature during the challenge.
journal_name
Vaccinejournal_title
Vaccineauthors
Kwon WJ,Choi JC,Hong S,Kim YC,Jeong MG,Min JG,Jeong JB,Kim KI,Jeong HDdoi
10.1016/j.vaccine.2020.11.001subject
Has Abstractpub_date
2020-12-03 00:00:00pages
8107-8115issue
51eissn
0264-410Xissn
1873-2518pii
S0264-410X(20)31423-7journal_volume
38pub_type
杂志文章相关文献
VACCINE文献大全abstract::Proteins isolated from the midgut of Glossina pallidipes were used to immunize rabbits and their efficacy as vaccine candidate(s) against the fly, and their potential to block transmission of Trypanosoma brucei rhodesiense assessed. Two fractions, detergent (DET) and aqueous (AQ) fractions were separated using a non-i...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.02.032
更新日期:2005-05-31 00:00:00
abstract:BACKGROUND:Micrurus venoms contain two main groups of toxic protein components: short-chain α-neurotoxins (SNtx) and phospholipases type A2 (PLA2). In North America, generally, the Micrurus venoms have low abundance of SNtx compared to that of PLA2s; however, both are highly toxic to mammals, and consequently both can ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.12.052
更新日期:2021-02-05 00:00:00
abstract::We have compared two types of plasmids for DNA immunization against HTLV-I envelope glycoproteins. One type of plasmid contains the coding DNA of the complete envelope gene of HTLV-I under the control of the CMV promoter with (CMVenvLTR) or without (CMVenv) the tax/rex genes. The second type contains the coding DNA of...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00565-4
更新日期:2000-04-28 00:00:00
abstract:BACKGROUND:Concern arose in 2010 that reactogenicity, particularly febrile seizures, to influenza A/H1N1-containing 2010-2011 trivalent seasonal inactivated influenza vaccine (TIV) could occur in young children who had been previously immunized and/or infected with the pandemic strain. We conducted a pre-season study o...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.vaccine.2012.03.046
更新日期:2012-05-14 00:00:00
abstract:OBJECTIVE:This case-control study investigated the relationship between the risk of Autism Spectrum Disorder (ASD) onset, and early exposure to the combined Measles-Mumps-Rubella (MMR) vaccine and thimerosal consumption measured from vaccinations in the highly genetically homogenous Japanese population. METHODS:Vaccin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.12.036
更新日期:2015-05-15 00:00:00
abstract::Fluid cholera vaccine was examined for its stability at 4-8 degrees C, 20-25 degrees C and 37 degrees C with regard to the number of organisms present and the total nitrogen content. Ten batches of fluid cholera vaccine manufactured at the Central Research Institute, Kasauli, India were taken for the study. The number...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(84)90046-x
更新日期:1984-12-01 00:00:00
abstract::Vaccines are everywhere hugely successful but are also under attack. The reason for the latter is the perception by some people that vaccines are unsafe. However that may be, vaccine safety, life any other scientific subject, must be constantly studied. It was from this point of view that a meeting was organized at th...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.01.024
更新日期:2020-02-18 00:00:00
abstract::Several approaches are being taken worldwide to develop vaccines against H5N1 viruses; most of them, however, pose both practical and immunological challenges. One potential strategy for improving the immunogenicity of vaccines involves the use of alphavirus replicons and VP22, a herpes simplex type 1 (HSV-1) protein....
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.05.014
更新日期:2009-12-09 00:00:00
abstract::The varied rates of protection induced by Mycobacterium bovis BCG vaccine against tuberculosis has been attributed to many factors such as genetic variability among BCG strains, rapid clearance of BCG in some populations, and different levels of previous exposure of vaccinated populations to environmental mycobacteria...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.12.044
更新日期:2012-02-01 00:00:00
abstract::In case of a bite by a rabies infected animal, the World Health Organisation recommends a prophylactic treatment including the administration of Human Rabies Immunoglobulins (HRIGs) or highly purified F(ab')2 fragments produced from Equine Rabies Immunoglobulin (F(ab')2 - ERIGs). According to international regulation,...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.04.086
更新日期:2016-06-14 00:00:00
abstract::Plasmid DNA is a promising vaccine platform that has been shown to be safe and able to be administered repeatedly without vector interference. Enhancing the potency of DNA vaccination through co-delivery of molecular adjuvants is one strategy currently under investigation. Here we describe the use of the novel chemoki...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.10.095
更新日期:2010-02-23 00:00:00
abstract::In Bangladesh, full vaccination rates among children living in rural hard-to-reach areas and urban streets are low. We conducted a quasi-experimental pre-post study of a 12-month mobile phone intervention to improve vaccination among 0-11 months old children in rural hard-to-reach and urban street dweller areas. Softw...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.11.024
更新日期:2016-01-04 00:00:00
abstract::Cholera is a potentially lethal diarrhea disease caused by the gram-negative bacterium Vibrio cholerae. The need for an effective cholera vaccine is clearly indicated but the challenges of eliciting both systemic and mucosal immune responses remains a significant challenge. In the current report, we discovered that a ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.04.008
更新日期:2009-06-12 00:00:00
abstract::Intact (146S) foot-and-mouth disease virions (FMDVs) can dissociate into specific (12S) viral capsid degradation products. Using two single-domain antibody fragments that bind specifically to either 146S or 12S particles we developed two ELISAs for the quantification of these particles in FMDV antigen preparations use...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.01.069
更新日期:2011-03-24 00:00:00
abstract:BACKGROUND:As part of the U.S. Department of Health and Human Services (HHS) Pandemic Influenza Plan preparedness and response strategy, the National Pre-Pandemic Influenza Vaccine Stockpile (NPIVS) program was established by the Biomedical Advanced Research and Development Authority (BARDA) in 2005 with the goal of bu...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2018.11.069
更新日期:2019-01-14 00:00:00
abstract::Recent studies have revealed that the interface of gp120 and gp41 and some parts of gp41 are also critical epitopes for elicitation of broadly neutralizing antibodies. Therefore, potential trimeric gp41 or gp140 immunogen candidates are needed. Previously, we developed a trimer motif MTQ and demonstrated that it could...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.09.077
更新日期:2020-11-03 00:00:00
abstract::Extensive glycosylation and variable loops of the HIV envelope protein (Env) are reported to shield some neutralizing epitopes. Here, we investigated the immunogenicity of mutated HIV Envs presented in virus-like particles (VLPs). We immunized mice with simian human immunodeficiency virus (SHIV) VLPs containing mutant...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.01.107
更新日期:2007-05-10 00:00:00
abstract::Globally the most commonly utilised immunisation against influenza is the trivalent inactivated influenza vaccine (TIV) derived from an A/H1N1, an A/H3N2 and aB type influenza virus. Vaccine effectiveness of TIV varies year to year, depending on how well antigenically matched the strains in the vaccine are compared to...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.09.062
更新日期:2017-11-07 00:00:00
abstract::We employed DNA shuffling and screening technologies to develop a single recombinant dengue envelope (E) antigen capable of inducing neutralizing antibodies against all four antigenically distinct dengue serotypes. By DNA shuffling of codon-optimized dengue 1-4 E genes, we created a panel of novel chimeric clones expr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.07.100
更新日期:2006-01-16 00:00:00
abstract::The membrane proximal region (MPR) of HIV-1 gp41 is a desirable target for development of a vaccine that elicits neutralizing antibodies since the patient-derived monoclonal antibodies, 2F5 and 4E10, bind to the MPR and neutralize primary HIV isolates. The 2F5 and 4E10 antibodies cross-react with lipids and structural...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.05.059
更新日期:2009-07-23 00:00:00
abstract::Enterotoxemia, a disease that affects domestic ruminants, is caused mainly by the epsilon toxin from Clostridium perfringens type D. Its eradication is virtually impossible, control and prophylaxis are based on systematic vaccination of herds with epsilon toxoids that are efficient in inducing protective antibody prod...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.07.046
更新日期:2010-08-31 00:00:00
abstract::Gag-CD8+ T cell responses are associated with immune control of HIV infection. Since during HIV infection Nef impairs T cell responses, we evaluated whether deletion of nef from a non-infectious HIV DNA vaccine (Delta4 Nef+), creating Delta5 Nef(-), would affect its immunogenicity. When compared with Delta4, mice inje...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.05.057
更新日期:2008-07-23 00:00:00
abstract:BACKGROUND:To support vaccine decision-making we estimated from the societal perspective the potential health impact and costs averted through immunization with three vaccines - Haemophilus influenzae type b (Hib), pneumococcal conjugate vaccine (PCV) and rotavirus vaccine (RVV). METHODS:Based on variability in diseas...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.09.084
更新日期:2019-12-10 00:00:00
abstract::Salmonella spp. are facultative intracellular pathogens causing localised or systemic infections, in addition to a chronic asymptomatic carrier state. They are of worldwide economic and public health significance. In poultry, which represent important sources of cheap protein throughout the world, fowl typhoid and pul...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/s0264-410x(99)00060-2
更新日期:1999-06-04 00:00:00
abstract::Current infant vaccination against pertussis in North America and Australia requires three doses of vaccines including diphtheria, tetanus and acellular pertussis antigens (DTaP) at 2, 4 and 6 months of age. Interest is growing in the possibility that vaccination at birth might provide earlier protection of infants, b...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.01.010
更新日期:2010-03-19 00:00:00
abstract::In this proof-of-concept study we report the use of <15 nm, water soluble, inorganic nanoparticles as a vaccine delivery system for a blood stage malaria vaccine. The recombinant malarial antigen, Merozoite Surface Protein 1 (rMSP1) of Plasmodium falciparum served as the model vaccine. The rMSP1 was covalently conjuga...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.09.070
更新日期:2011-11-08 00:00:00
abstract:BACKGROUND:Adjuvants like AS01B increase the immunogenicity of vaccines and generally cause increased transient reactogenicity compared with Alum. A phase II randomized trial was conducted to characterize the response to AS01B and Alum adjuvanted vaccines. A post-hoc analysis was performed to examine the associations b...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2019.02.015
更新日期:2019-03-28 00:00:00
abstract::Biological therapy options for the treatment of rheumatic disease target molecules that can affect the cross-talk between innate and adaptive immune responses upon vaccination. Influenza vaccination in children with rheumatic disease has been recommended, but there are only sparse data on the quality of vaccine respon...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.05.037
更新日期:2019-06-19 00:00:00
abstract::Age-specific force of varicella infection, hospitalisation and death rates in non-immune persons were calculated using an age-based mathematical model and national data for France. A cost-effectiveness model was then applied to hypothetical cohorts of persons aged 15-45 years with a negative or uncertain history of va...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(03)00405-5
更新日期:2003-09-08 00:00:00
abstract::Nasal mucosal barrier is a key impediment for the absorption of influenza whole inactivated virus (WIV) intranasal vaccine. Yet it is still unclear how WIV cross the epithelial cells (ECs) in nasal cavity. Here, in vitro, a coculture system was well established, consisting of surrogate nasal ECs (Calu-3) and dendritic...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.01.022
更新日期:2015-03-10 00:00:00