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A flow cytometric granularity assay for the quantification of infectious virus.

Abstract：

:A flow cytometry-based assay was developed to assess the infective titer of two recombinant viruses: a recombinant herpes simplex type 2 (rHSV-2) and a recombinant canary pox (rALVAC.gfp). This method uses granularity of infected Vero and QT-35 cells, respectively, and correlates this to the infectious titer of virus samples. The percent of the cell populations with a high level of granularity could accurately be correlated to viral titers obtained through a traditional plaque assay, with R2 values greater than 0.8 using a semi-logarithmic scale. This approach offers a rapid, high-throughput method for infectious virus titration with similar accuracy to a traditional plaque assay.

journal_name

Vaccine

journal_title

Vaccine

authors

Logan M,Manalil J,Notte C,Kearse C,George S,Zeiser A,Farrell P,Aucoin MG

doi

10.1016/j.vaccine.2019.02.059

subject

Has Abstract

pub_date

2019-11-08 00:00:00

pages

7090-7099

issue

47

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(19)30266-X

journal_volume

37

pub_type

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