Vaccinia virus proteins activate human dendritic cells to induce T cell responses in vitro.


:The mechanisms of protection elicited by smallpox vaccine in humans have not been delineated. Dendritic cells (DCs) are the most potent of professional antigen presenting cells which on activation modulate both innate and adaptive immune responses. Immunogenic proteins of vaccinia virus have been identified with protein microarray technology. We have investigated the effect of three most immunogenic proteins, D8L, D13L, and H3L on human monocyte-derived DCs with a view to understand the mechanisms underlying the immunogenicity of these proteins. Our data show that all three proteins activate and mature DCs, induce secretion of cytokines IL-12p70, IL-10, TNF-alpha, IL-6 from DCs, and rVV protein-loaded DCs induce secretion of IFN-gamma and proliferation of T cells with selective expansion of effector memory T cells. Overall D13L and H3L proteins are more immunogenic than D8L. Taken together data suggest that these peptides are highly immunogenic and can modulate an effective innate and adaptive response in humans in vitro.






Agrawal S,Gupta S,Agrawal A




Has Abstract


2009-01-01 00:00:00
















  • Development and optimisation of a procedure for the production of Parapoxvirus ovis by large-scale microcarrier cell culture in a non-animal, non-human and non-plant-derived medium.

    abstract::For the production of a chemically inactivated Parapoxvirus ovis (PPVO), an adherent bovine kidney cell line was cultivated on Cytodex-3 microcarriers in suspension culture. The inactivated and purified virus particles have shown immune modulatory activity in several animal models. PPVO was produced by a biphasic batc...


    pub_type: 杂志文章


    authors: Pohlscheidt M,Langer U,Minuth T,Bödeker B,Apeler H,Hörlein HD,Paulsen D,Rübsamen-Waigmann H,Henzler HJ,Reichl U

    更新日期:2008-03-17 00:00:00

  • RTS,S malaria vaccine efficacy and immunogenicity during Plasmodium falciparum challenge is associated with HLA genotype.

    abstract::Although RTS,S remains the most advanced malaria vaccine, the factors influencing differences in vaccine immunogenicity or efficacy between individuals or populations are still poorly characterised. The analyses of genetic determinants of immunogenicity have previously been restricted by relatively small sample sizes ...


    pub_type: 杂志文章


    authors: Nielsen CM,Vekemans J,Lievens M,Kester KE,Regules JA,Ockenhouse CF

    更新日期:2018-03-14 00:00:00

  • Immunogenicity and safety of rhesus-human rotavirus reassortant vaccines with serotype 1 or 2 VP7 specificity.

    abstract::Rhesus-human rotavirus reassortants incorporating the gene expressing the VP7 surface protein of human rotavirus serotypes 1 or 2, and the remaining ten genes from rhesus rotavirus (RRV) were evaluated as candidate oral vaccines in 2-4-month-old infants. A single dose of the serotype 1 reassortant vaccine which had a ...


    pub_type: 临床试验,杂志文章


    authors: Vesikari T,Varis T,Green K,Flores J,Kapikian AZ

    更新日期:1991-05-01 00:00:00

  • DNA vaccination with plasmids containing various fragments of large segment genome of infectious bursal disease virus.

    abstract::The present study was undertaken to determine the effectiveness in including VP2 gene of the large segment genome of infectious bursal disease virus (IBDV) in the DNA vaccine for protection of chickens against infectious bursal disease (IBD). Different fragments of the large segment gene of IBDV standard challenge (ST...


    pub_type: 杂志文章


    authors: Chang HC,Lin TL,Wu CC

    更新日期:2003-01-17 00:00:00

  • DNA immunization confers protective immunity on mice challenged intravaginally with herpes simplex virus type 2.

    abstract::The immunogenicity and protective efficacy of a nucleic acid vaccine encoding the herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) gene under the control of the CMV immediate early gene promoter was examined. Mice immunized three times by intramuscular injection with the vaccine developed an HSV specific IgG bu...


    pub_type: 杂志文章


    authors: Bourne N,Milligan GN,Schleiss MR,Bernstein DI,Stanberry LR

    更新日期:1996-09-01 00:00:00

  • Controlled human infections: A report from the controlled human infection models workshop, Leiden University Medical Centre 4-6 May 2016.

    abstract::The principle of deliberately infecting humans with infectious agents in a controlled setting, so-called controlled human infections (CHI), is not novel. Many CHI models have a long history and were established decades ago such as the intentional exposure to yellow fever and dengue performed in the 1900's (Reed, 1902)...


    pub_type: 杂志文章


    authors: Roestenberg M,Mo A,Kremsner PG,Yazdanbakhsh M

    更新日期:2017-12-18 00:00:00

  • Safety and immunogenicity of a quadrivalent human papillomavirus vaccine in HIV-infected and HIV-negative adolescents and young adults.

    abstract::Human papillomavirus (HPV) infection is highly prevalent and can lead to cancer; the development of safe and efficacious vaccines for HPV is a major public health concern. The two licensed HPV vaccines contain recombinant virus-like particles of HPV 16 and 18; one of such vaccines also protects against HPV types 6 and...


    pub_type: 杂志文章


    authors: Giacomet V,Penagini F,Trabattoni D,Viganò A,Rainone V,Bernazzani G,Bonardi CM,Clerici M,Bedogni G,Zuccotti GV

    更新日期:2014-09-29 00:00:00

  • In ovo administration of Toll-like receptor ligands encapsulated in PLGA nanoparticles impede tumor development in chickens infected with Marek's disease virus.

    abstract::One of the economically important diseases in the poultry industry is Marek's disease (MD) which is caused by Marek's disease virus (MDV). The use of current vaccines provides protection against clinical signs of MD in chickens. However, these vaccines do not prevent the transmission of MDV to susceptible hosts, hence...


    pub_type: 杂志文章


    authors: Bavananthasivam J,Alkie TN,Astill J,Abdul-Careem MF,Wootton SK,Behboudi S,Yitbarek A,Sharif S

    更新日期:2018-06-27 00:00:00

  • Propagation of Brazilian Zika virus strains in static and suspension cultures using Vero and BHK cells.

    abstract::The recent spread of Zika virus (ZIKV) in the Americas and the Pacific has reached alarming levels in more than 60 countries. However, relatively little is known about the disease on a virological and epidemiological level and its consequences for humans. Accordingly, a large demand for in vitro derived Brazilian ZIKV...


    pub_type: 杂志文章


    authors: Nikolay A,Castilho LR,Reichl U,Genzel Y

    更新日期:2018-05-24 00:00:00

  • Thymic atrophy in the mouse is a soluble problem of the thymic environment.

    abstract::Age related deterioration in the function of the immune system has been recognised in many species. The clinical presentations of such immune dysfunction are an age-related increased susceptibility to certain infections, and an increased incidence of autoimmune disease and certain cancers. Laboratory investigations re...


    pub_type: 杂志文章,评审


    authors: Aspinall R,Andrew D

    更新日期:2000-02-25 00:00:00

  • Sustained impact of rotavirus vaccine introduction on rotavirus gastroenteritis hospitalizations in children

    abstract:INTRODUCTION:Ghana introduced monovalent rotavirus vaccine in April 2012. We sought to determine the long-term impact of routine rotavirus vaccination on rotavirus gastroenteritis hospitalizations in Ghana during the first 4 years following rotavirus vaccine introduction. METHODS:Active sentinel surveillance for acute...


    pub_type: 杂志文章


    authors: Enweronu-Laryea CC,Armah G,Sagoe KW,Ansong D,Addo-Yobo E,Diamenu SK,Mwenda JM,Parashar UD,Tate JE

    更新日期:2018-11-12 00:00:00

  • Efficacy of a divalent and a multivalent water-in-oil formulated vaccine against a highly virulent strain of Flavobacterium psychrophilum after intramuscular challenge of rainbow trout (Oncorhynchus mykiss).

    abstract::Flavobacterium psychrophilum is a well-known pathogen causing significant problems in aquaculture worldwide. In recent years an increasing number of disease outbreaks caused by F. psychrophilum has been reported on juvenile and post smolts of rainbow trout (Oncorhynchus mykiss) in Norway. The current study was perform...


    pub_type: 杂志文章


    authors: Fredriksen BN,Olsen RH,Furevik A,Souhoka RA,Gauthier D,Brudeseth B

    更新日期:2013-04-08 00:00:00

  • Prevention of Coxiella burnetii shedding in infected dairy herds using a phase I C. burnetii inactivated vaccine.

    abstract::The main objective of this study was to assess the efficacy of a monovalent inactivated vaccine containing phase I Coxiella burnetii to prevent Coxiella shedding in susceptible dairy cows within infected herds in comparison to a placebo. A total of 336 dairy cows and heifers, from six spontaneously infected herds, wer...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Guatteo R,Seegers H,Joly A,Beaudeau F

    更新日期:2008-08-12 00:00:00

  • Development of a simple, sensitive, rapid test which discriminates BCG-vaccinated from Mycobacterium bovis-infected cattle.

    abstract::Bovine tuberculosis (bTB) is increasing in incidence in the UK. Effective control strategies could involve vaccination; BCG, either alone or in prime-boost strategies, remains the most effective vaccine against bovine tuberculosis. However, BCG vaccination of cattle would require development of diagnostic tests able t...


    pub_type: 杂志文章


    authors: Sopp P,Coad M,Hewinson G,Howard CJ,Cheallaigh CN,Keane J,Harris J,Hope JC

    更新日期:2008-10-09 00:00:00

  • The epidemiology and burden of rotavirus in China: a review of the literature from 1983 to 2005.

    abstract::We reviewed studies of rotavirus in MEDLINE and the Chinese literature to get a preliminary estimate of the burden of rotavirus gastroenteritis in China and the epidemiology of the disease. Studies were selected if they were conducted for a period 1 year or more, had more than 100 patients enrolled, and used an accept...


    pub_type: 杂志文章


    authors: Orenstein EW,Fang ZY,Xu J,Liu C,Shen K,Qian Y,Jiang B,Kilgore PE,Glass RI

    更新日期:2007-01-05 00:00:00

  • Impact of a mixed bacterial lysate (OM-85 BV) on the immunogenicity, safety and tolerability of inactivated influenza vaccine in children with recurrent respiratory tract infection.

    abstract::It is known that the immunogenicity and efficacy of conventional inactivated influenza vaccines (IIVs) are not completely satisfactory in children. The aim of this prospective, randomised, single-blind study was to compare the immune response to, and the effectiveness and safety of, an IIV (Fluarix, GlaxoSmithKline Bi...


    pub_type: 杂志文章,随机对照试验


    authors: Esposito S,Marchisio P,Prada E,Daleno C,Porretti L,Carsetti R,Bosco A,Ierardi V,Scala A,Principi N

    更新日期:2014-05-07 00:00:00

  • Modelling the epidemiological impact of rotavirus vaccination in Germany--a Bayesian approach.

    abstract:BACKGROUND:Rotavirus (RV) infection is the primary cause of severe gastroenteritis in children aged <5 years in Germany and worldwide. In 2013 the German Standing Committee on Vaccination (STIKO) developed a national recommendation for routine RV-immunization of infants. To support informed decision-making we predicted...


    pub_type: 杂志文章


    authors: Weidemann F,Dehnert M,Koch J,Wichmann O,Höhle M

    更新日期:2014-09-08 00:00:00

  • Immunogenicity and reactogenicity of co-administered tetanus-diphtheria-acellular pertussis (Tdap) and tetravalent meningococcal conjugate (MCV4) vaccines compared to their separate administration.

    abstract::In the United States, co-administration of tetanus toxoid, reduced diphtheria toxoid and acellular pertussis (Tdap) vaccine and tetravalent meningococcal conjugate vaccine (MCV4) is recommended in adolescents. In this clinical study, 1341 adolescents received Tdap (Boostrix® GlaxoSmithKline) and MCV4 (Menactra®, Sanof...


    pub_type: 杂志文章,随机对照试验


    authors: Weston WM,Friedland LR,Wu X,Howe B

    更新日期:2011-01-29 00:00:00

  • Induction of humoral immune response against Pseudomonas aeruginosa flagellin(1-161) using gold nanoparticles as an adjuvant.

    abstract::Flagellin of Pseudomonas aeruginosa is an important vaccine candidate. N-terminal domains are highly conserved in both type a and type b flagellins. The efficacy of gold nanoparticles (AuNPs) conjugated to N-terminal domains of P. aeruginosa flagellin (flagellin(1-161)), as an immunogen in mice, has been assessed. The...


    pub_type: 杂志文章


    authors: Dakterzada F,Mohabati Mobarez A,Habibi Roudkenar M,Mohsenifar A

    更新日期:2016-03-14 00:00:00

  • Correlates of HPV vaccination among adolescent females from Appalachia and reasons why their parents do not intend to vaccinate.

    abstract::Limited research has examined HPV vaccination in Appalachia, a region with cervical cancer disparities. We analyzed 2008-2010 National Immunization Survey-Teen data for adolescent females ages 13-17 from Appalachia (n=1951) to identify correlates of HPV vaccination and reasons why their parents do not intend to vaccin...


    pub_type: 杂志文章


    authors: Reiter PL,Katz ML,Paskett ED

    更新日期:2013-06-28 00:00:00

  • Development and preclinical safety evaluation of a new therapeutic HIV-1 vaccine based on 18 T-cell minimal epitope peptides applying a novel cationic adjuvant CAF01.

    abstract::Therapeutic immunization of HIV-1-infected individuals with or without anti-retroviral therapy is a new promising disease prevention. To induce a new cytotoxic T(CD8) lymphocyte (CTL) immunity during chronic HIV-1 infection 15 infrequently targeted but conserved HLA-supertype binding CTL epitopes from Gag, Pol, Nef, E...


    pub_type: 杂志文章


    authors: Fomsgaard A,Karlsson I,Gram G,Schou C,Tang S,Bang P,Kromann I,Andersen P,Andreasen LV

    更新日期:2011-09-16 00:00:00

  • Safety and immunogenicity of unadjuvanted subvirion monovalent inactivated influenza H3N2 variant (H3N2v) vaccine in children and adolescents.

    abstract:OBJECTIVE:In response to the emergence of influenza viruses with pandemic potential, we evaluated a swine-origin influenza A/H3N2 variant (H3N2v) vaccine in children. STUDY DESIGN:This multicenter phase II open-label study assessed the safety and immunogenicity of two doses, 21 days apart, of investigational unadjuvan...


    pub_type: 杂志文章


    authors: Munoz FM,Anderson EJ,Bernstein DI,Harrison CJ,Pahud B,Anderson E,Creech CB,Berry AA,Kotloff KL,Walter EB,Atmar RL,Bellamy AR,Chang S,Keitel WA

    更新日期:2019-08-23 00:00:00

  • Progress towards hepatitis B prevention through vaccination in the Western Pacific, 1990-2014.

    abstract::Hepatitis B infections are responsible for more than 300 thousand deaths per year in the Western Pacific Region. Because of this high burden, the countries and areas of the Region established a goal of reducing hepatitis B chronic infection prevalence among children to less than 1% by 2017. This study was conducted to...


    pub_type: 杂志文章,评审


    authors: Wiesen E,Diorditsa S,Li X

    更新日期:2016-05-27 00:00:00

  • Applications of bioinformatics and computational biology to influenza surveillance and vaccine strain selection.

    abstract::In recent years, collaborations often between mathematical and computational biologists and scientists in the World Health Organization (WHO) global influenza surveillance network, have resulted in a number of mathematical and computational advances including: increasing the resolution at which antigenic surveillance ...


    pub_type: 杂志文章


    authors: Smith DJ

    更新日期:2003-05-01 00:00:00

  • Improving immunization data quality in Peru and Mexico: Two case studies highlighting challenges and lessons learned.

    abstract:INTRODUCTION:The Global Vaccine Action Plan and the Regional Immunization Action Plan of the Americas call for countries to improve immunization data quality. Immunization information systems, particularly electronic immunization registries (EIRs), can help to facilitate program management and increase coverage. Howeve...


    pub_type: 杂志文章


    authors: Trumbo SP,Contreras M,García AGF,Díaz FAE,Gómez M,Carrión V,Ruiz KJP,Aquije R,Danovaro-Holliday MC,Velandia-González M

    更新日期:2018-11-29 00:00:00

  • Identification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans.

    abstract::Toxoplasma gondii is an intracellular parasite that causes severe neurologic and ocular disease in immune-compromised and congenitally infected individuals. There is no vaccine protective against human toxoplasmosis. Herein, immunization of L(d) mice with HF10 (HPGSVNEFDF) with palmitic acid moieties or a monophosphor...


    pub_type: 杂志文章


    authors: Tan TG,Mui E,Cong H,Witola WH,Montpetit A,Muench SP,Sidney J,Alexander J,Sette A,Grigg ME,Maewal A,McLeod R

    更新日期:2010-05-21 00:00:00

  • Induction of T-cell response by a DNA vaccine encoding a novel HLA-A*0201 severe acute respiratory syndrome coronavirus epitope.

    abstract::The severe acute respiratory syndrome coronavirus nucleocapsid protein (SARS-CoV N) is one of the major targets for SARS vaccine due to its high potency in triggering immune responses. In this study, we have identified a novel HLA-A*0201 restricted epitope, N220 (LALLLLDRL), of the SARS-CoV N-protein through bioinform...


    pub_type: 杂志文章


    authors: Cheung YK,Cheng SC,Sin FW,Chan KT,Xie Y

    更新日期:2007-08-10 00:00:00

  • Is the pneumococcal vaccine Advance Market Commitment motivating innovation and increasing manufacturing capacity? Some preliminary answers.

    abstract::This paper seeks to give some preliminary evidence on the potential outcome of the pneumococcal vaccine Advance Market Commitment (AMC), with a focus on its impact on innovation in 'emerging' vaccine manufacturers in developing countries. The evidence is derived from a series of interviews with executives at industria...


    pub_type: 杂志文章


    authors: Plahte J

    更新日期:2012-03-23 00:00:00

  • Poly(propyleneimine) dendrimer and dendrosome mediated genetic immunization against hepatitis B.

    abstract::The purpose of the present research work is to explore the potential of dendrosomes in genetic immunization against hepatitis B. Plasmid DNA encoding pRc/CMV-HBs[S] (5.6 kb), encoding the small region of the hepatitis B surface antigen, was complexed with 5th generation poly(propyleneimine) dendrimer (PPI) in differen...


    pub_type: 杂志文章


    authors: Dutta T,Garg M,Jain NK

    更新日期:2008-06-25 00:00:00

  • Antibodies to measles, mumps and rubella in UK children 4 years after vaccination with different MMR vaccines.

    abstract::Persistence of antibodies 4 years after vaccination with measles, mumps and rubella (MMR) vaccine from three different manufacturers was compared in 475 children who received a single injection of vaccine when aged 12-18 months. Antibodies to measles and mumps were measured using a plaque reduction neutralisation assa...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Miller E,Hill A,Morgan-Capner P,Forsey T,Rush M

    更新日期:1995-06-01 00:00:00