Abstract:
:Dengue virus (DENV) is the causal agent of severe disease and, in some cases, mortality in humans, but no licensed vaccines against dengue are available. An effective vaccine against dengue requires long-term humoral and cellular immune responses. Several viral proteins have been the subjects of intense research, especially the envelope (E) protein, aimed at developing a vaccine. Domain III of the envelope protein (EDIII) has been identified as a potential candidate because it is involved in binding to host cell receptors and contains epitopes that elicit virus neutralizing antibodies. However, this domain is not sufficiently antigenic when is expressed and administered as antigen to elicit a strong immune response. One alternative to enhance immunogenicity is to target the antigen to dendritic cells to induce T-cells for broad antibody responses. In this work, a single chain antibody fragment (scFv) raised against the DEC-205 receptor fused with the EDIII was successfully expressed in Nicotiana benthamiana. The recombinant protein was expressed and purified from the plant and evaluated in BALB/c mice to test its immunogenicity and ability to induce neutralizing antibodies against DENV. The mice immunized with the recombinant protein produced specific and strong humoral immune responses to DENV. Only two immunizations were required to generate a memory response to DENV without the presence of adjuvants. Also, recognition of the recombinant protein with sera from DENV-infected patients was observed. These findings suggest that this strategy has potential for development of an effective vaccine against DENV.
journal_name
Vaccinejournal_title
Vaccineauthors
Coconi-Linares N,Ortega-Dávila E,López-González M,García-Machorro J,García-Cordero J,Steinman RM,Cedillo-Barrón L,Gómez-Lim MAdoi
10.1016/j.vaccine.2013.03.009subject
Has Abstractpub_date
2013-05-01 00:00:00pages
2366-71issue
19eissn
0264-410Xissn
1873-2518pii
S0264-410X(13)00268-5journal_volume
31pub_type
杂志文章相关文献
VACCINE文献大全abstract::Vaccination is an effective strategy to prevent pneumococcal diseases. Currently, licensed vaccines include the pneumococcal polysaccharide vaccine (PPSV) and the pneumococcal conjugate vaccine (PCV), which target some of the most common of the 94 serotypes of S. pneumoniae based on their capsular composition. However...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.11.015
更新日期:2019-01-03 00:00:00
abstract::The effects of beta-propiolactone (BPL), an alkylating and virus inactivating agent, on the structural and in vitro biological properties of different DNA preparations from BHK-21 cells were investigated. Both uninfected and rabies virus-infected cells were used. Purified cellular DNA (celDNA) was used as the referenc...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90343-v
更新日期:1993-01-01 00:00:00
abstract::Mycoplasma hyopneumoniae (M. hyopneumoniae) vaccine strain 168 is an intrapulmonically injected attenuated live vaccine that is available in the Chinese market. The aim of this study was to develop suitable adjuvants for this live vaccine to provide effective protection after intramuscular inoculation. Several adjuvan...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.03.071
更新日期:2014-06-05 00:00:00
abstract::Hepatitis B surface antigen (HBsAg) was detected in 3.3% of 7162 pregnant Tunisian women tested and HBeAg in 9.6% of the HBsAg-positive mothers. Family members of 46 of these HBsAg-positive mothers (33 husbands and 61 children aged 1-6 years) were investigated for the presence of HBV markers. HBsAg was detected in 21%...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90205-4
更新日期:1994-01-01 00:00:00
abstract::Gram-negative bacterial outer membrane proteins (Omps) have an important role in pathogenesis and signal reception. We previously reported that Acinetobacter OmpA (AbOmpA) induced maturation of bone marrow-derived dendritic cells (BMDCs) and that AbOmpA-primed DCs produced IL-12 which generated Th1 CD4(+) T-cells. We ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.01.036
更新日期:2011-03-16 00:00:00
abstract::Currently, commercial porcine rotavirus vaccines remain varied limitations. The objective of this study is to develop an alternative porcine rotavirus subunit vaccine candidate by parenteral administration, which enables to elicit robust immune responses against most prevalence porcine rotavirus strains. The bacterial...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.07.011
更新日期:2015-08-26 00:00:00
abstract::A strategy to improve the immunogenicity of candidate vaccines is to trigger the innate immune system. Triggering of CD40 at the surface of dendritic cells (DC) is essential in the induction of an efficient immune response. Although CD40 agonist antibodies have been shown to be potent inducers of immune responses in e...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.05.028
更新日期:2008-07-29 00:00:00
abstract::Limited research has examined HPV vaccination in Appalachia, a region with cervical cancer disparities. We analyzed 2008-2010 National Immunization Survey-Teen data for adolescent females ages 13-17 from Appalachia (n=1951) to identify correlates of HPV vaccination and reasons why their parents do not intend to vaccin...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.04.068
更新日期:2013-06-28 00:00:00
abstract::Glycoconjugate vaccines play an enormous role in preventing infectious diseases. The main carrier proteins used in commercial conjugate vaccines are the non-toxic mutant of diphtheria toxin (CRM197), diphtheria toxoid (DT) and tetanus toxoid (TT). Modern childhood routine vaccination schedules include the administrati...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.11.026
更新日期:2015-01-03 00:00:00
abstract::Hybrid cell vaccines of autologous tumour cells fused with allogenic dendritic cells (DC) combine the tumour's antigenicity with the immune-stimulatory capacity of mature dendritic cells and allogenic MHC class II molecules to activate T cell help and induce tumour-specific cytotoxic T cells. This concept was tested i...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/j.vaccine.2005.01.081
更新日期:2005-03-18 00:00:00
abstract:BACKGROUND:Influenza A/H7N9 viruses are undergoing antigenic drift since their emergence in 2013, and vaccination strategies are needed for pandemic preparedness. Two doses of adjuvanted monovalent inactivated influenza A/H7N9 vaccine (IIV1 A/H7N9) are needed for optimal serological responses. However, administering 2 ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.03.071
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:Adverse events following immunization (AEFI) requires special consideration in patients with primary immunodeficiency diseases (PID) because they may represent a "red flag" for the initial diagnosis and may cause disease complications. Therefore, the definition of appropriate vaccination schemes is a major i...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.01.047
更新日期:2016-03-18 00:00:00
abstract::The need for developing a case definition and guidelines for a local reaction at or near the injection site, methods for the development of the case definition and guidelines as an adverse event following immunization as well as the rationale for selected decisions about the case definition for a local reaction at or ...
journal_title:Vaccine
pub_type: 指南,杂志文章
doi:10.1016/j.vaccine.2008.10.006
更新日期:2008-12-09 00:00:00
abstract::Concerns about bioterrorism and outbreaks of zoonotic orthopoxvirus require safe and efficacious smallpox vaccines. We previously reported the clinical efficacy and safety profiles of LC16m8, a live, attenuated, cell culture-derived, smallpox vaccine, examined in over 3000 healthy Japanese adults with various vaccinat...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.09.006
更新日期:2019-10-16 00:00:00
abstract::Long-term protection of rabbits that had been vaccinated with two doses of a recombinant protective antigen (rPA) vaccine was examined against an aerosol spore challenge with the Ames isolate of Bacillus anthracis at 6 and 12 months. At 6 months after the primary injection, survival was 74.1% (20/27) with quantitative...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.12.028
更新日期:2006-03-24 00:00:00
abstract::We evaluated immunologic predictors of response to HBV vaccine administered in the presence or absence of GM-CSF in HIV infected individuals. We measured peripheral blood hematopoietic progenitor, monocyte and myeloid-derived suppressor cell (MDSC) frequencies, and expression of GMCSF receptor on monocytes and MDSCs, ...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2011.02.092
更新日期:2011-04-27 00:00:00
abstract::Inactivated influenza vaccine (Ivac) has had an important impact on reducing attack rates of influenza and reducing the severity of illness amongst the vaccinees who still acquire infection. Ivac is most efficacious amongst young, otherwise healthy subjects and least effective against elderly at high risk. This is in ...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0264-410x(99)00508-3
更新日期:2000-02-25 00:00:00
abstract::Influenza causes considerable morbidity and mortality and the damage to public health can be considerable. The most effective measures available for the prevention of influenza is vaccination. In most industrialised countries the objective of vaccination is to limit the disease among individuals at risk, especially th...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(02)00507-8
更新日期:2002-12-20 00:00:00
abstract::Salmonella is one of the key global causes of food and water borne enteric infections, responsible for significant morbidity and mortality worldwide especially in developing countries. Currently available vaccines against typhoid are moderately effective with several side effects and not efficacious against all Salmon...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.05.106
更新日期:2018-06-27 00:00:00
abstract:BACKGROUND:Roughly half of U.S. adults do not receive recommended booster vaccinations, but protective antibody levels are rarely measured in adults. Demographic factors, vaccination history, and responses to other vaccinations could help identify at-risk individuals. We sought to characterize rates of seroconversion a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.08.005
更新日期:2014-09-29 00:00:00
abstract::Respiratory mucosa immunization is capable of eliciting both local and distal mucosal immune responses; it is a potentially powerful yet largely unused modality for vaccination against respiratory diseases. Targeting the lower versus upper airways by aerosol delivery alters the immunogenicity profile of a vaccine, alt...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.12.008
更新日期:2017-01-23 00:00:00
abstract::Amebiasis remains one of the leading parasitic causes of death worldwide. A vaccine that prevented amebic liver abscess would significantly reduce mortality from this disease. To test the feasibility of a DNA vaccine to prevent amebic liver abscess, we immunized both mice and gerbils with plasmid DNA encoding the seri...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00343-6
更新日期:1999-12-10 00:00:00
abstract::Streptococcus pneumoniae is an important pathogen worldwide. Accurate sampling of S. pneumoniae carriage is central to surveillance studies before and following conjugate vaccination programmes to combat pneumococcal disease. Any bias introduced during sampling will affect downstream recovery and typing. Many variable...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2012.08.080
更新日期:2012-11-06 00:00:00
abstract::Class I molecules are conformationally sensitive to peptide binding, prolonging the complex's half-life on the surface of the cell. By making a series of H2-Kb anchor motif amino acid point substitutions in the ovalbumin 257-264 octamer, we were able to analyse subtle changes in peptide binding, Kb stabilization and i...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(95)93320-9
更新日期:1995-02-01 00:00:00
abstract::A phase II safety and immunogenicity study of an oral-formalin inactivated enterotoxigenic Escherichia coli (ETEC) vaccine containing six colonization factors (CFA/I, CS1, CS2, CS3, CS4, CS5) and 1mg of recombinant cholera toxin B subunit (the CF-BS-ETEC vaccine) was carried out in an urban slum of Dhaka city in Bangl...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/s0264-410x(03)00077-x
更新日期:2003-06-02 00:00:00
abstract::Our initial results with a bovine papilloma virus (BPV) vector expression system indicated that we could produce significant amounts of Epstein-Barr virus (EBV) gp340/220 in the supernatant of a mouse fibroblast cell line. We have now extended these findings to show that the truncated version of gp340/220, where the m...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(92)90513-j
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:We have shown that the intradermal (ID) administration of an HIV-1 lipopeptide candidate vaccine (LIPO-4) is well tolerated in healthy volunteers, with one fifth the IM dose delivered by this route inducing HIV-1-specific CD8(+) T-cell responses of a magnitude and quality similar to those achieved by IM admi...
journal_title:Vaccine
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.vaccine.2013.06.102
更新日期:2013-09-13 00:00:00
abstract::The effect of passively transferred RSV immune serum on the antibody response to a single dose of purified RSV fusion (F) and large (G) glycoproteins was studied in cotton rats. Passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eig...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(91)90151-u
更新日期:1991-03-01 00:00:00
abstract::It has been known that influenza A virus infection induces a cross-protective immunity against infection by viruses with different subtypes of viral envelope proteins, hemagglutinin (HA) and neuraminidase (NA). This heterosubtypic immunity is generally mediated by cytotoxic T lymphocytes (CTL) reactive to specific epi...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(03)00234-2
更新日期:2003-07-04 00:00:00
abstract::The immunity levels against diphtheria, tetanus and poliomyelitis were investigated among blood donors (n = 2079) in Berlin. Of all participants, only 60% had full, long-term protection against diphtheria, 72% against tetanus, 87% against poliomyelitis type 1, 77% against poliomyelitis type 2 and 73% against type 3. T...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(98)00269-2
更新日期:1999-02-26 00:00:00