Effect of different adjuvant formulations on the immunogenicity and protective effect of a live Mycoplasma hyopneumoniae vaccine after intramuscular inoculation.


:Mycoplasma hyopneumoniae (M. hyopneumoniae) vaccine strain 168 is an intrapulmonically injected attenuated live vaccine that is available in the Chinese market. The aim of this study was to develop suitable adjuvants for this live vaccine to provide effective protection after intramuscular inoculation. Several adjuvant components were screened to assess their toxicity for the live vaccine, and various adjuvant formulations were then designed and prepared. Vaccines supplemented with these adjuvants were used to immunize mice intramuscularly to assess the capacity of the adjuvants to induce a specific immune response. The screened formulations were then evaluated in pigs. Seven of the eight adjuvant components did not affect the viability of the live vaccine, and seven different adjuvant formulations were then designed. In mice, the ISCOM-matrix adjuvant and the levamisole-chitosan mixture adjuvant significantly enhanced serum IgG responses against M. hyopneumoniae, while lymphocyte proliferation was enhanced by the ISCOM-matrix adjuvant, the carbomer-astragalus polysaccharide mixture adjuvant and an oil-in-water emulsion adjuvant. These four adjuvants were evaluated in pigs. Enhancement of specific lymphocyte proliferation responses was observed in the groups vaccinated with the ISCOM-matrix adjuvant and the carbomer-astragalus polysaccharide mixture adjuvant. Significant enhancement of serum IgG antibody production was observed before challenge in pigs vaccinated with the carbomer-astragalus polysaccharide mixture adjuvant and the levamisole-chitosan mixture adjuvant, while after challenge, all of the animals that received vaccines containing adjuvants had higher antibody concentrations against M. hyopneumoniae than unvaccinated animals. Animals inoculated with a vaccine containing the ISCOM-matrix adjuvant (median score 3.57) or the carbomer-astragalus polysaccharide mixture adjuvant (median score 5.28) had reduced lesion scores compared to unvaccinated animals (median score 14.81). These studies will help in the development of appropriate adjuvants for intramuscular administration of this live M. hyopneumoniae vaccine.






Xiong Q,Wei Y,Xie H,Feng Z,Gan Y,Wang C,Liu M,Bai F,Xie F,Shao G




Has Abstract


2014-06-05 00:00:00
















  • Healthy individuals' immune response to the Bulgarian Crimean-Congo hemorrhagic fever virus vaccine.

    abstract::Crimean-Congo hemorrhagic fever virus (CCHFV) poses a great threat to public health due to its high mortality and transmission rate and wide geographical distribution. There is currently no specific antiviral therapy for CCHF. This study provides the first in-depth analysis of the cellular and humoral immune response ...


    pub_type: 杂志文章


    authors: Mousavi-Jazi M,Karlberg H,Papa A,Christova I,Mirazimi A

    更新日期:2012-09-28 00:00:00

  • Studies on the usefulness of intranasal inactivated influenza vaccines.

    abstract::Intranasal inactivated influenza vaccines have the advantage over parenteral vaccines in that they are not associated with the pain of an injection. However, they would be most useful if they were available for all age groups, including high-risk groups, and also would provide cross-protection against variant virus st...


    pub_type: 杂志文章,评审


    authors: Tamura S

    更新日期:2010-08-31 00:00:00

  • Applying an equity lens in the Decade of Vaccines.

    abstract::Addressing inequities in immunisation must be the main priority for the Decade of Vaccines. Children who remain unreached are those who need vaccination - and other health services - most. Reaching these children and other underserved target groups will require a reorientation of current approaches and resource alloca...


    pub_type: 杂志文章,评审


    authors: Brearley L,Eggers R,Steinglass R,Vandelaer J

    更新日期:2013-04-18 00:00:00

  • Safety and immunogenicity of a quadrivalent inactivated influenza vaccine in adults.

    abstract:BACKGROUND AND AIMS:Although two antigenically distinct B strain lineages of influenza have co-circulated globally since the mid-1980s, trivalent influenza vaccines (TIVs) contain only one, resulting in frequent mismatches. This study examined the safety and immunogenicity of an inactivated quadrivalent influenza vacci...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Pépin S,Donazzolo Y,Jambrecina A,Salamand C,Saville M

    更新日期:2013-11-12 00:00:00

  • Effectiveness of a physician peer educator in improving the quality of immunization services for young children in primary care practices.

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    pub_type: 杂志文章,随机对照试验


    authors: Taylor JA,Rietberg K,Greenfield L,Bibus D,Yasuda K,Marcuse EK,Duchin JS

    更新日期:2008-08-05 00:00:00

  • Bactericidal antibody against a representative epidemiological meningococcal serogroup B panel confirms that MATS underestimates 4CMenB vaccine strain coverage.

    abstract:BACKGROUND:4CMenB (Bexsero), a vaccine developed against invasive meningococcal disease caused by capsular group B strains (MenB), was recently licensed for use by the European Medicines Agency. Assessment of 4CMenB strain coverage in specific epidemiologic settings is of primary importance to predict vaccination impac...


    pub_type: 杂志文章


    authors: Frosi G,Biolchi A,Lo Sapio M,Rigat F,Gilchrist S,Lucidarme J,Findlow J,Borrow R,Pizza M,Giuliani MM,Medini D

    更新日期:2013-10-09 00:00:00

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    pub_type: 杂志文章


    authors: Douce G,Giuliani MM,Giannelli V,Pizza MG,Rappuoli R,Dougan G

    更新日期:1998-07-01 00:00:00

  • Safety and immunogenicity of unadjuvanted subvirion monovalent inactivated influenza H3N2 variant (H3N2v) vaccine in children and adolescents.

    abstract:OBJECTIVE:In response to the emergence of influenza viruses with pandemic potential, we evaluated a swine-origin influenza A/H3N2 variant (H3N2v) vaccine in children. STUDY DESIGN:This multicenter phase II open-label study assessed the safety and immunogenicity of two doses, 21 days apart, of investigational unadjuvan...


    pub_type: 杂志文章


    authors: Munoz FM,Anderson EJ,Bernstein DI,Harrison CJ,Pahud B,Anderson E,Creech CB,Berry AA,Kotloff KL,Walter EB,Atmar RL,Bellamy AR,Chang S,Keitel WA

    更新日期:2019-08-23 00:00:00

  • Alterations in serotype-specific B cell responses to the 13-valent pneumococcal conjugate vaccine in aging HIV-infected adults.

    abstract:BACKGROUND:Advanced age and human immunodeficiency virus (HIV) infection are associated with increased pneumococcal disease risk. The impact of these factors on cellular responses to vaccination is unknown. METHODS:HIV-infected (HIV+) individuals 50-65 years old with CD4(+) Tcells/μl (CD4) >200 on antiretroviral thera...


    pub_type: 杂志文章


    authors: Ohtola JA,Khaskhely NM,Saul-Mcbeth JL,Iyer AS,Leggat DJ,Khuder SA,Westerink MAJ

    更新日期:2016-01-20 00:00:00

  • An experimental marker vaccine and accompanying serological diagnostic test both based on envelope glycoprotein E2 of classical swine fever virus (CSFV).

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    pub_type: 杂志文章


    authors: van Rijn PA,van Gennip HG,Moormann RJ

    更新日期:1999-02-05 00:00:00

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    abstract::The characterization of the cross-reactive, or heterologous, neutralizing antibody (NAb) responses developed during HIV-1-infection is relevant to the development of an HIV vaccine. Here we summarize our recently published study where we characterized the cross-reactive NAb responses in two cohorts of anti-retroviral ...


    pub_type: 杂志文章


    authors: Sather DN,Stamatatos L

    更新日期:2010-05-26 00:00:00

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    abstract::T-lymphocyte responses to influenza vaccination were measured in healthy young and older adult volunteers. All participants were vaccinated with the 1995-96 trivalent influenza vaccine. Cytokine and granzyme B levels were measured in peripheral blood mononuclear cells (PBMC) cultures after virus stimulation, prior to ...


    pub_type: 杂志文章


    authors: McElhaney JE,Upshaw CM,Hooton JW,Lechelt KE,Meneilly GS

    更新日期:1998-11-01 00:00:00

  • Semantic network analysis of vaccine sentiment in online social media.

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    pub_type: 杂志文章


    authors: Kang GJ,Ewing-Nelson SR,Mackey L,Schlitt JT,Marathe A,Abbas KM,Swarup S

    更新日期:2017-06-22 00:00:00

  • Nature, evolution, and appraisal of adverse events and antibody response associated with the fifth consecutive dose of a five-component acellular pertussis-based combination vaccine.

    abstract::We performed a randomized, controlled clinical trial to characterize the evolution of the adverse events associated with the fifth consecutive dose of an acellular pertussis vaccine, and to assess the level of discomfort associated with the injection and the attitude of parents concerning these events. A total of 505 ...


    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Halperin SA,Scheifele D,Mills E,Guasparini R,Humphreys G,Barreto L,Smith B

    更新日期:2003-06-02 00:00:00

  • Immune response to two different dosing schedules of the anthrax vaccine precipitated (AVP) vaccine.

    abstract::A pilot study compared the immune response of regular (0, 3, 6, 32 weeks) and extended (0, 10, 13, 32 weeks) schedules of the UK anthrax vaccine (anthrax vaccine precipitated, AVP). Concentrations of antibodies to protective antigen (PA) were higher (p<0.05) among recipients of the extended (n=7) versus regular schedu...


    pub_type: 杂志文章


    authors: Hepburn MJ,Hugh Dyson E,Simpson AJ,Brenneman KE,Bailey N,Wilkinson L,Hornby R,Mateczun AJ,Bell MG,Baillie LW

    更新日期:2007-08-10 00:00:00

  • Protection against tick-borne encephalitis virus isolated in Japan by active and passive immunization.

    abstract::In order to establish a firm preventive measure for tick-borne encephalitis (TBE) in Japan, we evaluated the immune response of European vaccine against Japanese TBE virus strain (Oshima 5-10) for man and mouse. Furthermore, the efficacy of pre- and post-exposure protection by a polyclonal rabbit anti-TBE virus serum ...


    pub_type: 杂志文章


    authors: Chiba N,Osada M,Komoro K,Mizutani T,Kariwa H,Takashima I

    更新日期:1999-03-17 00:00:00

  • Immunogenicity of an alum-adsorbed synthetic multiple-antigen peptide based on B- and T-cell epitopes of the Plasmodium falciparum CS protein: possible vaccine application.

    abstract::Multiple-antigen peptides (MAPs), containing B- and T-cell epitopes of the Plasmodium falciparum circumsporozoite (CS) protein, have been designed to overcome the limitations of first-generation peptide vaccines caused by low epitope density, carrier toxicity and the lack of parasite-derived T-cell epitopes. The immun...


    pub_type: 杂志文章


    authors: de Oliveira GA,Clavijo P,Nussenzweig RS,Nardin EH

    更新日期:1994-08-01 00:00:00

  • Perspectives on the control of hepatitis A by vaccination.

    abstract::The availability of an inactivated hepatitis A virus (HAV) vaccine, and the development of live attenuated virus vaccines against hepatitis represent great advances in the effort to control an important cause of viral hepatitis. There are a number of ways hepatitis A vaccines could be used, depending on the epidemiolo...


    pub_type: 杂志文章,评审


    authors: Kane MA

    更新日期:1992-01-01 00:00:00

  • Evaluating the effectiveness of national measles elimination action in mainland China during 2004-2016: A multi-site interrupted time-series study.

    abstract:BACKGROUND:To block transmission of measles, the China Ministry of Health endorsed the "2006-2012 National Measles Elimination Action Plan (NMEAP)" on November 11, 2006, while no published studies used mathematical models to assess the impact of this action. Also, the problem of residuals autocorrelation due to auto-co...


    pub_type: 杂志文章


    authors: Xu L,Chen J,Zhan Z,Chen T,Liu W,Qian J,Ou C

    更新日期:2020-06-09 00:00:00

  • Feasibility of using regional sentinel surveillance to monitor the rotavirus vaccine impact, effectiveness and intussusception incidence in the African Region.

    abstract::The 9th African rotavirus symposium was held in Maputo, Mozambique from the 8th to 10th of December 2015, including a total of 101 delegates from 17 countries, 15 of which were African countries. This forum brought together participants with various expertise including scientists, clinicians, immunization program mana...


    pub_type: 杂志文章


    authors: Mandomando I,Weldegebriel G,de Deus N,Mwenda JM

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  • Specific memory B cell response and participation of CD4+ central and effector memory T cells in mice immunized with liposome encapsulated recombinant NE protein based Hepatitis E vaccine candidate.

    abstract:BACKGROUND:Liposome encapsulated neutralizing epitope protein of Hepatitis E virus (HEV), rNEp, our Hepatitis E vaccine candidate, was shown to be immunogenic and safe in pregnant and non-pregnant mice and yielded sterilizing immunity in rhesus monkeys. METHODS:The current study in Balb/c mice assessed the levels and ...


    pub_type: 杂志文章


    authors: Kulkarni SP,Thanapati S,Arankalle VA,Tripathy AS

    更新日期:2016-11-21 00:00:00

  • Presentation of caregiver-specific vaccine-related information on National Cancer Institute designated cancer center websites.

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    pub_type: 杂志文章


    authors: Marellapudi A,King AR,Bednarczyk RA

    更新日期:2020-09-11 00:00:00

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    pub_type: 杂志文章,评审


    authors: Salek-Ardakani S,Croft M

    更新日期:2006-02-13 00:00:00

  • Immunogenicity and tolerability of an HPV-16/18 AS04-adjuvanted prophylactic cervical cancer vaccine in women aged 15-55 years.

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    pub_type: 杂志文章,多中心研究


    authors: Schwarz TF,Spaczynski M,Schneider A,Wysocki J,Galaj A,Perona P,Poncelet S,Zahaf T,Hardt K,Descamps D,Dubin G,HPV Study Group for Adult Women.

    更新日期:2009-01-22 00:00:00

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    pub_type: 杂志文章


    authors: Bonomo ME,Kim RY,Deem MW

    更新日期:2019-05-27 00:00:00

  • Levofloxacin rescues mice from lethal intra-nasal infections with virulent Francisella tularensis and induces immunity and production of protective antibody.

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    pub_type: 杂志文章


    authors: Klimpel GR,Eaves-Pyles T,Moen ST,Taormina J,Peterson JW,Chopra AK,Niesel DW,Carness P,Haithcoat JL,Kirtley M,Nasr AB

    更新日期:2008-12-09 00:00:00

  • Invasive pneumococcal diseases in birth cohorts vaccinated with PCV-7 and/or PHiD-CV in the province of Quebec, Canada.

    abstract:BACKGROUND:The 10-valent protein D pneumococcal conjugate vaccine (PHiD-CV) was licensed on the basis of immunogenicity studies and there are no published data on its effectiveness to prevent invasive pneumococcal disease (IPD). In the province of Quebec, Canada, PHiD-CV was introduced in the summer of 2009, replacing ...


    pub_type: 杂志文章


    authors: De Wals P,Lefebvre B,Defay F,Deceuninck G,Boulianne N

    更新日期:2012-10-05 00:00:00

  • O-acetylation of typhoid capsular polysaccharide confers polysaccharide rigidity and immunodominance by masking additional epitopes.

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    pub_type: 杂志文章


    authors: Hitri K,Kuttel MM,De Benedetto G,Lockyer K,Gao F,Hansal P,Rudd TR,Beamish E,Rijpkema S,Ravenscroft N,Bolgiano B

    更新日期:2019-06-27 00:00:00

  • An improved Pythium insidiosum-vaccine formulation with enhanced immunotherapeutic properties in horses and dogs with pythiosis.

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    pub_type: 杂志文章


    authors: Mendoza L,Mandy W,Glass R

    更新日期:2003-06-20 00:00:00

  • Clinical proof of principle for ChimeriVax: recombinant live, attenuated vaccines against flavivirus infections.

    abstract::ChimeriVax is a live, attenuated recombinant virus constructed from yellow fever (YF) 17D in which the envelope protein genes of YF 17D are replaced with the corresponding genes of another flavivirus. A ChimeriVax vaccine was developed against Japanese encephalitis (JE). A randomized, double-blind, outpatient study wa...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Monath TP,McCarthy K,Bedford P,Johnson CT,Nichols R,Yoksan S,Marchesani R,Knauber M,Wells KH,Arroyo J,Guirakhoo F

    更新日期:2002-01-15 00:00:00