Mucosal immunization against hepatitis A: antibody responses are enhanced by co-administration of synthetic oligodeoxynucleotides and a novel cationic lipid.


:Hepatitis A caused by hepatitis A virus (HAV) transmitted by the fecal-oral route, results in considerable morbidity and economic loss. Mucosal immunization can be more effective than conventional injection at inducing both local and systemic immunity to HAV. Here we show that co-administration of killed HAV with synthetic oligodeoxynucleotides (ODNs) containing CpG sequences, and a novel polycationic sphingolipid (CCS)/cholesterol liposomal delivery system, markedly enhances the HAV-specific antibody response at the intestinal interface, particularly when delivered intrarectally or intranasally, to Balb/c mice at low HAV doses. A mucosally delivered, antigen-sparing HAV vaccine that is easily administered without specialized equipment or personnel, is an attractive alternative for facilitating mass immunization in hepatitis A outbreaks.






Mitchell LA,Joseph A,Kedar E,Barenholz Y,Galun E




Has Abstract


2006-06-19 00:00:00
















  • Use of administrative records to assess pneumococcal conjugate vaccine impact on pediatric meningitis and pneumonia hospitalizations in Rwanda.

    abstract:BACKGROUND:Ongoing surveillance is critical to assessing pneumococcal conjugate vaccine (PCV) impact over time. However, robust prospective studies are difficult to implement in resource-poor settings. We evaluated retrospective use of routinely collected data to estimate PCV impact in Rwanda. METHODS:We collected dat...


    pub_type: 杂志文章


    authors: Gatera M,Uwimana J,Manzi E,Ngabo F,Nwaigwe F,Gessner BD,Moïsi JC

    更新日期:2016-10-17 00:00:00

  • Translating vaccine policy into action: a report from the Bill & Melinda Gates Foundation Consultation on the prevention of maternal and early infant influenza in resource-limited settings.

    abstract::Immunization of pregnant women against influenza is a promising strategy to protect the mother, fetus, and young infant from influenza-related diseases. The burden of influenza during pregnancy, the vaccine immunogenicity during this period, and the robust influenza vaccine safety database underpin recommendations tha...


    pub_type: 共识发展会议,杂志文章


    authors: Ortiz JR,Neuzil KM,Ahonkhai VI,Gellin BG,Salisbury DM,Read JS,Adegbola RA,Abramson JS

    更新日期:2012-11-26 00:00:00

  • Adjuvant Quil A improves protection in mice and enhances opsonic capacity of antisera induced by pneumococcal polysaccharide conjugate vaccines.

    abstract::The adjuvant effect of Quil A on the primary antibody response of mice to pneumococcal capsular polysaccharide conjugates was examined. Quil A increased the anti-capsular polysaccharide antibody titres, the protection against Streptococcus pneumoniae, and the opsonic capacity of the antibodies as measured in a newly d...


    pub_type: 杂志文章


    authors: DeVelasco EA,Dekker HA,Antal P,Jalink KP,van Strijp JA,Verheul AF,Verhoef J,Snippe H

    更新日期:1994-11-01 00:00:00

  • Pools of lipidated HTL-CTL constructs prime for multiple HBV and HCV CTL epitope responses.

    abstract::Various peptide-based approaches to simultaneous induction of multiple cytotoxic T lymphocyte (CTL) responses were evaluated as part of ongoing efforts to develop immunotherapeutic vaccines for use in humans. To this end, HLA (human histocompatibility leukocyte antigen)-A2-restricted epitopes from several specific vir...


    pub_type: 杂志文章


    authors: Oseroff C,Sette A,Wentworth P,Celis E,Maewal A,Dahlberg C,Fikes J,Kubo RT,Chesnut RW,Grey HM,Alexander J

    更新日期:1998-05-01 00:00:00

  • Prospects for preventing infant invasive GBS disease through maternal vaccination.

    abstract::Group B streptococcus (GBS) is a leading cause of neonatal sepsis, with the highest incidence (1.3 per 1000 live births) reported from Africa. Although the incidence of invasive GBS disease is reportedly low in South Asia, there is disconnect between prevalence of maternal recto-vaginal colonization and the incidence ...


    pub_type: 杂志文章


    authors: Madhi SA,Dangor Z

    更新日期:2017-08-16 00:00:00

  • Effectiveness of mouse brain-derived inactivated Japanese encephalitis vaccine in Thai National Immunization Program: a case-control study.

    abstract::Mouse brain-derived, purified inactivated Japanese encephalitis vaccine (MB JEV) has been locally produced and introduced into Thai National Immunization Program (NIP) since 1990. However, MB JEV effectiveness has been concerned, since 30-40% of JE cases received JE vaccines were recently reported in two descriptive s...


    pub_type: 杂志文章


    authors: Muangchana C,Henprasertthae N,Nurach K,Theppang K,Yoocharoen P,Varinsathien P,Techathawat S,Sanohsieng S,Anantapreecha S

    更新日期:2012-01-05 00:00:00

  • Reducing pain at the time of vaccination: WHO position paper, September 2015-Recommendations.

    abstract::This article presents the World Health Organization's (WHO) recommendations for pain mitigation at the time of vaccination from the WHO position paper on reducing pain at the time of vaccination: WHO position paper-September 2015, recently published in the Weekly Epidemiological Record [1]. This position paper summari...


    pub_type: 杂志文章


    authors: WHO.

    更新日期:2016-07-12 00:00:00

  • Mapping financial flows for immunisation in Uganda 2009/10 and 2010/11: New insights for methodologies and policy.

    abstract:BACKGROUND:The Global Vaccine Action Plan highlights the need for immunisation programmes to have sustainable access to predictable funding. A good understanding of current and future funding needs, commitments, and gaps is required to enhance planning, improve resource allocation and mobilisation, and to avoid funding...


    pub_type: 杂志文章


    authors: Guthrie T,Zikusooka C,Kwesiga B,Abewe C,Lagony S,Schutte C,Marinda E,Humphreys K,Motlogelwa K,Nombewu ZC,Brenzel L,Kinghorn A

    更新日期:2015-05-07 00:00:00

  • Indicators of influenza and pneumococcal vaccination in French nursing home residents in 2011.

    abstract:INTRODUCTION:Older adults living in nursing homes (NH) are at high risk of developing influenza and pneumococcal infections. The objectives of this study were to describe vaccination coverage for influenza and pneumococcal among French NH residents and to investigate which NH structure- and organisation-related aspects...


    pub_type: 杂志文章


    authors: de Souto Barreto P,Lapeyre-Mestre M,Vellas B,Rolland Y

    更新日期:2014-02-07 00:00:00

  • Novel use of a N2-specific enzyme-linked immunosorbent assay for differentiation of infected from vaccinated animals (DIVA)-based identification of avian influenza.

    abstract::Proper vaccination with validated companion differentiation of infected from vaccinated animals (DIVA) tests using a vaccine containing a heterologous neuraminidase to the field virus can be effective to control avian influenza (AI). However, indirect immunofluorescent assay, the only field validated DIVA test, has li...


    pub_type: 杂志文章


    authors: Kwon JS,Kim MC,Jeong OM,Kang HM,Song CS,Kwon JH,Lee YJ

    更新日期:2009-05-21 00:00:00

  • Pneumococcal conjugate serotype distribution and predominating role of serotype 3 in German adults with community-acquired pneumonia.

    abstract:INTRODUCTION:Implementation of the 7-valent pneumococcal conjugate vaccine (PCV7) in infant vaccination programs has substantially reduced the burden of PCV7 serotypes also in adult community-acquired pneumonia (CAP). Currently, it is unclear, if this extensive herd protection effect can be extrapolated to the addition...


    pub_type: 杂志文章


    authors: Forstner C,Kolditz M,Kesselmeier M,Ewig S,Rohde G,Barten-Neiner G,Rupp J,Witzenrath M,Welte T,Pletz MW,CAPNETZ study group.

    更新日期:2020-01-29 00:00:00

  • Design and content validation of a survey questionnaire assessing the determinants of human papillomavirus (HPV) vaccine hesitancy in France: A reactive Delphi study.

    abstract:INTRODUCTION:This study aimed to develop and undertake a preliminary validation of a French Survey Questionnaire for the Determinants of HPV Vaccine Hesitancy (FSQD-HPVH). METHODS:We undertook an electronic-based Delphi consultation among a panel of Francophone experts in two rounds. Round 1 consisted of the assessmen...


    pub_type: 杂志文章


    authors: Dib F,Mayaud P,Launay O,Chauvin P,FSQD-HPVH Study Group.

    更新日期:2020-09-03 00:00:00

  • Beta-propiolactone treatment impairs the biological activity of residual DNA from BHK-21 cells infected with rabies virus.

    abstract::The effects of beta-propiolactone (BPL), an alkylating and virus inactivating agent, on the structural and in vitro biological properties of different DNA preparations from BHK-21 cells were investigated. Both uninfected and rabies virus-infected cells were used. Purified cellular DNA (celDNA) was used as the referenc...


    pub_type: 杂志文章


    authors: Morgeaux S,Tordo N,Gontier C,Perrin P

    更新日期:1993-01-01 00:00:00

  • A robust lentiviral pseudotype neutralisation assay for in-field serosurveillance of rabies and lyssaviruses in Africa.

    abstract::The inflexibility of existing serological techniques for detection of rabies in surveillance constrains the benefit to be gained from many current control strategies. We analysed 304 serum samples from Tanzanian dogs for the detection of rabies antibodies in a pseudotype assay using lentiviral vectors bearing the CVS-...


    pub_type: 杂志文章


    authors: Wright E,McNabb S,Goddard T,Horton DL,Lembo T,Nel LH,Weiss RA,Cleaveland S,Fooks AR

    更新日期:2009-11-27 00:00:00

  • Persistence of antibodies to meningococcal IgA1 protease versus decay of antibodies to group A polysaccharide and Opc protein.

    abstract::Sera were taken over a 5 year period from Gambian children vaccinated in 1983, when aged 1-4 years, with A + C meningococcal capsular polysaccharide, ELISA tests were devised to determine the concentrations of immunoglobulin A, G and M reacting with A polysaccharide and of IgG reacting with Opc protein, IgA1 protease ...


    pub_type: 杂志文章


    authors: Thiesen B,Greenwood B,Brieske N,Achtman M

    更新日期:1997-02-01 00:00:00

  • Vaccine coverage and adherence to EPI schedules in eight resource poor settings in the MAL-ED cohort study.

    abstract:BACKGROUND:Launched in 1974, the Expanded Program on Immunization (EPI) is estimated to prevent two-three million deaths annually from polio, diphtheria, tuberculosis, pertussis, measles, and tetanus. Additional lives could be saved through better understanding what influences adherence to the EPI schedule in specific ...


    pub_type: 杂志文章


    authors: Hoest C,Seidman JC,Lee G,Platts-Mills JA,Ali A,Olortegui MP,Bessong P,Chandyo R,Babji S,Mohan VR,Mondal D,Mahfuz M,Mduma ER,Nyathi E,Abreu C,Miller MA,Pan W,Mason CJ,Knobler SL,MAL-ED Network Investigators.

    更新日期:2017-01-11 00:00:00

  • Nef modulates the immunogenicity of Gag encoded in a non-infectious HIV DNA vaccine.

    abstract::Gag-CD8+ T cell responses are associated with immune control of HIV infection. Since during HIV infection Nef impairs T cell responses, we evaluated whether deletion of nef from a non-infectious HIV DNA vaccine (Delta4 Nef+), creating Delta5 Nef(-), would affect its immunogenicity. When compared with Delta4, mice inje...


    pub_type: 杂志文章


    authors: Arrode G,Hegde R,Jin Y,Singh DK,Narayan O,Chebloune Y

    更新日期:2008-07-23 00:00:00

  • Targeted expression of HTLV-I envelope proteins in muscle by DNA immunization of mice.

    abstract::We have compared two types of plasmids for DNA immunization against HTLV-I envelope glycoproteins. One type of plasmid contains the coding DNA of the complete envelope gene of HTLV-I under the control of the CMV promoter with (CMVenvLTR) or without (CMVenv) the tax/rex genes. The second type contains the coding DNA of...


    pub_type: 杂志文章


    authors: Armand MA,Grange MP,Paulin D,Desgranges C

    更新日期:2000-04-28 00:00:00

  • AIDS and HIV vaccines.

    abstract::In spite of extensive prevention programs, the HIV epidemics is still spreading worldwide, in particularly in developing countries where clade C viruses predominate. WHO estimates that there are 16, 000 new cases of HIV infection daily and that 100 M individuals will be infected by the end of the next decade. In spite...


    pub_type: 杂志文章,评审


    authors: Klein M

    更新日期:1999-10-01 00:00:00

  • Murine response to DNA encoding herpes simplex virus type-1 glycoprotein D targeted to the liver.

    abstract::Plasmid DNA encoding herpes simplex virus type-1 glycoprotein D (gD-1) was complexed with asialoorosomucoid conjugated to poly-L-lysine. Following its intravenous injection into BALB/c mice, this complex was targeted to the liver. Liver cells expressing gD-1 were detected immunohistochemically through day 6 post-immun...


    pub_type: 杂志文章


    authors: Rogers JV,Hull BE,Fink PS,Chiou HC,Bigley NJ

    更新日期:2000-02-14 00:00:00

  • Determinants of willingness to pay for self-paid vaccines in China.

    abstract:BACKGROUND:While vaccines not covered by China's Expanded Program on Immunization can be received voluntarily with out-of-pocket payment, the uptake of self-paid vaccines in China is low. OBJECTIVE:To investigate willingness to pay (WTP) for self-paid vaccines and its determinants in China. METHODS:We interviewed 216...


    pub_type: 杂志文章


    authors: Hou Z,Jie Chang,Yue D,Fang H,Meng Q,Zhang Y

    更新日期:2014-07-31 00:00:00

  • Safety of yellow fever vaccine administration in confirmed egg-allergic patients.

    abstract::Yellow fever vaccine (YFV) is recommended in endemic areas but represents a risk for egg-allergic patients, as it is cultivated in embryonated eggs. This study aims to describe the outcomes of yellow fever vaccination in patients with confirmed egg allergy (EA). Methods:A prospective study was conducted from January 2...


    pub_type: 杂志文章


    authors: Gerhardt CMB,Castro APBM,Pastorino AC,Dorna MB,Nunes-Santos CJ,Aquilante BP,Miyaji KT,Lopes MH

    更新日期:2020-09-29 00:00:00

  • Secondary in vitro B lymphocyte (antibody) response to microbial antigens: use in appraisal of vaccine immunogenicity and cytokine immunoregulation.

    abstract::In order to perform preliminary evaluations of subunit vaccine candidates before extensive trials in large food-producing animals, an in vitro B-lymphocyte response assay, based on the principles of an ELISA, was established. The assay was developed in detail for the porcine system using antigens from the Gram-negativ...


    pub_type: 杂志文章


    authors: Ohmann HB,McDougall L,Potter A

    更新日期:1991-03-01 00:00:00

  • Haptoglobin polymorphism and the immune response after hepatitis B vaccination.

    abstract::One hundred healthy Caucasian medical students (age 22 +/- 1 years) were vaccinated with a recombinant hepatitis B vaccine and their haptoglobin types were determined. A relationship between haptoglobin type and immune response to the vaccine was observed. Subjects with a 2-2 haptoglobin phenotype produced significant...


    pub_type: 临床试验,杂志文章


    authors: Louagie H,Delanghe J,Desombere I,De Buyzere M,Hauser P,Leroux-Roels G

    更新日期:1993-09-01 00:00:00

  • Effects of a multi-faceted program to increase influenza vaccine uptake among health care workers in nursing homes: A cluster randomised controlled trial.

    abstract::Despite the recommendation of the Dutch association of nursing home physicians (NVVA) to be immunized against influenza, vaccine uptake among HCWs in nursing homes remains unacceptably low. Therefore we conducted a cluster randomised controlled trial among 33 Dutch nursing homes to assess the effects of a systematical...


    pub_type: 杂志文章,随机对照试验


    authors: Looijmans-van den Akker I,van Delden JJ,Verheij TJ,van der Sande MA,van Essen GA,Riphagen-Dalhuisen J,Hulscher ME,Hak E

    更新日期:2010-07-12 00:00:00

  • Delivery to the lower respiratory tract is required for effective immunization with Newcastle disease virus-vectored vaccines intended for humans.

    abstract::Newcastle disease virus (NDV), an avian virus, is being evaluated for the development of vectored human vaccines against emerging pathogens. Previous studies of NDV-vectored vaccines in a mouse model suggested their potency after delivery by injection or by the intranasal route. We compared the efficacy of various rou...


    pub_type: 杂志文章


    authors: DiNapoli JM,Ward JM,Cheng L,Yang L,Elankumaran S,Murphy BR,Samal SK,Collins PL,Bukreyev A

    更新日期:2009-03-04 00:00:00

  • The impact of private use of PCV7 in 2009 and 2010 on serotypes and antimicrobial resistance of Streptococcus pneumoniae carried by young children in Portugal: Comparison with data obtained since 1996 generating a 15-year study prior to PCV13 introduction

    abstract::In Portugal, the 7-valent pneumococcal conjugate vaccine (PCV7) was not introduced in the national immunization plan but was commercially available between 2001 and 2010. We studied serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae carried by children in 2009 and 2010. Vaccination with PC...


    pub_type: 杂志文章


    authors: Nunes S,Félix S,Valente C,Simões AS,Tavares DA,Almeida ST,Paulo AC,Brito-Avô A,de Lencastre H,Sá-Leão R

    更新日期:2016-03-29 00:00:00

  • Development of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens.

    abstract::The objective of the present study was to investigate the feasibility of a subunit vaccine and a live bacteria vaccine to protect chickens against infectious bursal disease virus (IBDV) infection. The gene for VP2 of a new wild-type very virulent IBDV (vvIBDV) strain was cloned into an Escherichia coli expression syst...


    pub_type: 杂志文章


    authors: Rong J,Cheng T,Liu X,Jiang T,Gu H,Zou G

    更新日期:2005-09-23 00:00:00

  • Immunological solutions for treatment and prevention of porcine reproductive and respiratory syndrome (PRRS).

    abstract::Vaccination is the principal means used to control and treat porcine reproductive and respiratory syndrome virus (PRRSV) infection. An array of PRRS vaccine products is available in various regions of the world. However, despite extensive efforts, little progress has been made to improve efficacy since the first intro...


    pub_type: 杂志文章,评审


    authors: Murtaugh MP,Genzow M

    更新日期:2011-10-26 00:00:00

  • Protection of pigs against Taenia solium cysticercosis by immunization with novel recombinant antigens.

    abstract::Recombinant antigens from the oncosphere stage of the parasite Taenia solium were expressed in Escherichia coli. The TSOL16, TSOL45-1A and TSOL45-1B recombinant antigens, each consisting of fibronectin type III (FnIII) domain S, were produced as fusion proteins with glutathione S-transferase (GST) and maltose binding ...


    pub_type: 杂志文章


    authors: Gauci CG,Jayashi CM,Gonzalez AE,Lackenby J,Lightowlers MW

    更新日期:2012-06-06 00:00:00