Abstract:
:In this study, in vivo electroporation of a DNA vaccine adjuvanted with plasmids encoding different cytokines was investigated in large animals. Sheep were injected intramuscularly with a DNA vaccine encoding an antigen of Haemonchus contortus (pNPA) and plasmids encoding different cytokines followed by in vivo electroporation. Plasmids (pCI) carrying the genes of different cytokines including ovine IL-4(pCI-IL4), IL-10(pCI-IL10), GM-CSF(pCI-GMCSF), and MCP-1alpha(pCI-MCP1alpha), and pCI-IL4+pCI-GMCSF were co-delivered with pNPA. The results showed that co-delivery of pCI-GMCSF or pCI-IL4+pCI-GMCSF significantly enhanced both antibody responses and T cell proliferation responses to the antigen after two DNA immunisations compared to co-delivery of pCI. In contrast, antibody responses of the sheep that received pCI-IL10 were decreased significantly. Other cytokine expressing plasmids did not significantly alter the measured immune responses. Furthermore, co-delivery of pCI-GMCSF increased IgG2 response more than IgG1 responses, suggesting a Th1 bias. However, the increase in IgG2 over IgG1 was less apparent when co-delivery of pCI-IL4 with pCI-GMCSF. Interestingly, the co-delivery of pCI-IL4 alone did not increase the IgG1 titre, suggesting that both pCI-GMCSF and pCI-IL4 are required for optimal IgG1 production. Thus, co-delivery of plasmid-encoded cytokine genes with in vivo electroporation has the ability to effectively modulate immune responses to a DNA vaccine in a large animal.
journal_name
Vaccinejournal_title
Vaccineauthors
Yen HH,Scheerlinck JPdoi
10.1016/j.vaccine.2006.12.025subject
Has Abstractpub_date
2007-03-30 00:00:00pages
2575-82issue
14eissn
0264-410Xissn
1873-2518pii
S0264-410X(06)01357-0journal_volume
25pub_type
杂志文章相关文献
VACCINE文献大全abstract::The role of disulfide-dependent protein conformation of the 195,000 kDa Plasmodium falciparum merozoite surface glycoprotein in the induction of biologically active antibodies was investigated. Serum samples from rabbits immunized with native gp195 had a mean ELISA titre of 1/560,000 and a mean in vitro parasite growt...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90072-6
更新日期:1993-01-01 00:00:00
abstract::Varied doses of Taishan Pinus massoniana pollen polysaccharide (TPPPS) and Astragalus polysaccharide (APS) extracted by hot water extraction and ethanol precipitation method were added to the vaccine in order to prepare polysaccharide-rabbit haemorrhagic disease (RHD) tissue inactivated vaccine. The purpose was to stu...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.01.068
更新日期:2011-03-21 00:00:00
abstract::The continuously increasing demand for potent and safe vaccines and the intensifying economic pressure on health care systems underlines the need for further optimization of vaccine manufacturing. Here, we focus on downstream processing of human influenza vaccines, investigating the purification of serum-free cell cul...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.06.086
更新日期:2018-05-24 00:00:00
abstract:BACKGROUND:Most developing countries are implementing the WHO immunisation programme. Although vaccines reach most children, many modifications of the recommended schedule are observed in practice. We investigated the association between vaccination status and risk of hospitalisation in Guinea-Bissau. METHODS:From May...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.03.016
更新日期:2011-05-09 00:00:00
abstract:BACKGROUND:Rotavirus is a leading cause of childhood morbidity and mortality worldwide. Clinical trials for two rotavirus vaccines recommended by the WHO for global use since 2009 have successfully demonstrated the safety and efficacy of these vaccines in a wide range of countries. To control the burden of severe and f...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.08.058
更新日期:2012-10-12 00:00:00
abstract::Clostridium difficile infection (CDI) causes nosocomial antibiotic-associated diarrhea and colitis in the developed world. Two potent cytotoxins, toxin A (TcdA) and toxin B (TcdB) are the virulence factors of this disease and can be a good vaccine candidate against CDI. In the present study, we genetically engineered ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.02.006
更新日期:2015-03-24 00:00:00
abstract::A mass vaccination campaign against influenza A/H1N1 was launched in France in October 2009. Vaccination was offered free of charge to the entire population according to a pre-defined order of priority. Demographic data and data on vaccinations given were recorded in a dedicated database. We analysed vaccine uptake by...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.09.096
更新日期:2010-11-29 00:00:00
abstract:OBJECTIVES:We aimed to estimate vaccine effectiveness against severe measles based on the number of vaccine doses administered and the time since last vaccination. PATIENTS AND METHODS:We included measles cases aged at least 2 years and born since 1980 who were notified in France between 2006 and mid-2019. We consider...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.05.005
更新日期:2020-06-15 00:00:00
abstract::Here we confirm that intranasal (IN) dry powder anthrax vaccine formulations are able to protect rabbits against aerosol challenge 9 weeks after a single immunization. The optimum dose of rPA in our dry powder anthrax vaccine formulation in rabbits was experimentally determined to be 150microg and therefore was chosen...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.07.062
更新日期:2008-10-09 00:00:00
abstract::Recombinant canarypox (CP) and fowlpox (FP) viruses that contained two forms of the HIV-1 (SF2 strain) env gene were engineered and their expression analysed in chick, simian and human cells. These vectors can efficiently replicate in avian but not in mammalian cells, in which infection is abortive. The two forms, con...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90180-5
更新日期:1994-09-01 00:00:00
abstract::We investigated the therapeutic effects of romurtide, a synthetic muramyl dipeptide derivative, on experimental thrombocytopenia induced by carboplatin in cynomolgus monkeys. A prolonged thrombocytopenia due to a severe myelosuppression was induced by carboplatin. Romurtide given subcutaneously elevated significantly ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(96)00064-3
更新日期:1996-10-01 00:00:00
abstract::In the present study, we have produced recombinant paraflagellar rod proteins (PFR) and report their use for successful vaccination of mice against Trypanosoma cruzi. This protection is associated with a highly polarized type 1 cytokine production profile. Additionally, we have analyzed the gene sequence encoding PFR-...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(03)00108-7
更新日期:2003-06-20 00:00:00
abstract::Travellers often present late for the third dose of hepatitis B vaccination and measuring anti-HBs serology is common practice to ensure that these individuals are protected. We assessed the adequacy of immune response in 86 healthy travellers who had their third dose of hepatitis B (HB) vaccine performed more than 1 ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.12.053
更新日期:2007-04-30 00:00:00
abstract::Actinobacillus pleuropneumoniae is an important respiratory pathogen of swine, for which there is no highly effective vaccine. A phage expression library of the A. pleuropneumoniae genome was constructed and screened to identify potential vaccine components. Open reading frames within immuno-reactive phage were analyz...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.02.022
更新日期:2008-04-07 00:00:00
abstract:BACKGROUND:The world's first dengue vaccine [Dengvaxia; Sanofi Pasteur] was licensed in 2015 and others are in development. Real-world evaluations of dengue vaccines will therefore soon be needed. We assessed feasibility of case control (CC) and test-negative (TN) design studies for dengue vaccine effectiveness by meas...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2019.07.083
更新日期:2019-09-16 00:00:00
abstract:BACKGROUND:Worldwide coverage of hepatitis B (HB) vaccination is increasing. This should be considered when determining the best strategy for catch-up HB vaccination in migrant children, who rarely have written proof of past immunizations. This study aimed to estimate HB vaccine protection, chronic HB prevalence and to...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.06.010
更新日期:2018-07-16 00:00:00
abstract::Immunisation is one of the corner stones of public health. Most health care consumers see the health care worker as their major source of information on immunisation and vaccine safety. Doctors, nurses and midwives should be appropriately and timely trained for that role. Within the Vaccine Safety, Attitudes, Training...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.12.033
更新日期:2010-02-25 00:00:00
abstract::Pneumococcal infection is a leading cause of adult morbidity and mortality in the United States. Immunization against this infection is a valuable strategy in preventive medicine. An effective pneumococcal vaccine has been available in this country for over a decade, but it remains poorly utilized. The purpose of the ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90239-9
更新日期:1994-10-01 00:00:00
abstract::The ability to readily elicit broadly neutralizing antibodies to HIV-1 remains elusive. We and others have hypothesized that interaction of the viral envelope glycoprotein (Env, gp120-gp41) with its receptor molecules could enhance the exposure of conserved epitopes that may facilitate the elicitation of broadly neutr...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(03)00494-8
更新日期:2003-10-01 00:00:00
abstract::Malaria transmission-blocking vaccines (TBV) targeting sexual stages of the parasite represent an ideal intervention to reduce the burden of the disease and eventual elimination at the population level in endemic regions. Immune responses against sexual stage antigens impair the development of parasite inside the mosq...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.08.025
更新日期:2015-09-22 00:00:00
abstract::Peripheral blood mononuclear cells (PBMCs) from unvaccinated and vaccinated bovine calves were employed to study the alteration of different T-cell subpopulations, functional competence and detection of foot-and-mouth disease virus (FMDV) at different hours post in vitro infection (hpi) with FMDV serotype either O or ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.08.107
更新日期:2009-11-12 00:00:00
abstract::In 2013, avian H7N9 influenza viruses were detected infecting people in China resulting in high mortality. Influenza H7 vaccines that provide cross-protection against these new viruses are needed until specific H7N9 vaccines are ready to market. In this study, an available H7N3 cold-adapted, temperature sensitive, liv...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2014.11.008
更新日期:2015-01-01 00:00:00
abstract::Seneca Valley virus (SVV) infection in pigs is associated with porcine idiopathic vesicular disease (PIVD). Outbreaks of SVV infection in pig herds have been reported in several Asia and Americas countries. Recently, a series of outbreaks of SVV infection occurred in China, Canada, Thailand and the United States. Howe...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.12.055
更新日期:2018-02-01 00:00:00
abstract::Oral polio vaccine (OPV) and diphtheria-tetanus-pertussis (DTP) vaccines are given simultaneously in routine immunisation programmes in developing countries. It is therefore difficult to determine the separate effects of these vaccines on survival. We used the shortage of DTP vaccine in Bissau to examine the impact of...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.02.009
更新日期:2004-08-13 00:00:00
abstract::To date, most peptide-based vaccines evaluated for the treatment of cancer have consisted of one or few peptides. However, as a greater number of peptide antigens become available for use in experimental therapies, it is important to establish the feasibility of combining multi-peptide reagents as individual peptide m...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.01.018
更新日期:2009-03-10 00:00:00
abstract:BACKGROUND:The objective of this study was to estimate the number of years after onset of a quadrivalent HPV vaccination program before notable reductions in genital warts and cervical intraepithelial neoplasia (CIN) will occur in teenagers and young adults in the United States. METHODS:We applied a previously publish...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.06.050
更新日期:2013-08-20 00:00:00
abstract::The case-centered approach (CCA) can be useful in vaccine safety studies to adjust for time varying confounders, especially seasonality. The method compares the observed odds of vaccination in the time interval prior to the event with the expected odds of vaccination during this interval. The log of the expected odds ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.07.064
更新日期:2013-01-02 00:00:00
abstract::Financial sustainability plans (FSPs) were developed by over 50 of the world's poorest countries receiving funding support from the Global Alliance for Vaccines and Immunization (GAVI) to introduce new and underused vaccines, injection safety and immunization service support between 2000 and 2006. These plans were ana...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2008.10.014
更新日期:2008-12-02 00:00:00
abstract::Protective mechanisms underlying the responses to mucosal vaccination are not yet clearly defined. Using the natural mouse pneumovirus pathogen, pneumonia virus of mice (PVM), we explore responses of wild type and interferon-gamma (IFNgamma) receptor gene-deleted mice to virulent challenge after mucosal vaccination wi...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.09.081
更新日期:2007-01-22 00:00:00
abstract::We explore cellular and molecular mechanisms of nasal adjuvant of a combination of a plasmid encoding the Flt3 ligand cDNA (pFL) and CpG oligodeoxynucleotides (CpG ODN). The double DNA adjuvant given with OVA maintained prolonged OVA-specific secretory IgA (S-IgA) Ab responses in external secretions for more than 25 w...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.06.091
更新日期:2008-09-02 00:00:00