Expression of HIV-1 envelope gene by recombinant avipox viruses.


:Recombinant canarypox (CP) and fowlpox (FP) viruses that contained two forms of the HIV-1 (SF2 strain) env gene were engineered and their expression analysed in chick, simian and human cells. These vectors can efficiently replicate in avian but not in mammalian cells, in which infection is abortive. The two forms, consisting of the entire env open reading frame (IS+) or of the same gene lacking the putative immunosuppressive (IS-) region (amino acids 583-599), were individually inserted into the two virus vector backgrounds. In order to avoid premature transcription termination of the foreign gene and to improve protein expression, a mutagenesis was also performed within the T5NT motif without altering the amino acid sequence. By immunoprecipitation analyses, cells infected with CP and FP recombinants expressed HIV-1 env polypeptides of the appropriate molecular weight. We observed that the gp160 precursor was proteolytically cleaved except in MRC-5 cells infected with the IS- recombinants and that these polypeptides were glycosylated. Further analysis of these recombinant viruses by indirect immunofluorescence and syncytia inhibition assays indicated that the gp120 gp41 complex was present on the surface of infected cells, the number of syncytia being significantly lower when cells were infected by the CPIS- or FPIS- recombinants. Moreover, sera of immunized rabbits revealed the presence of specific antibodies in animals inoculated either with CP or with FP recombinants. These new constructs, which are unable to support a productive infection in human cells, might therefore also be a good anti-HIV-1 candidate vaccine in seropositive hosts.






Radaelli A,De Giuli Morghen C




Has Abstract


1994-09-01 00:00:00
















  • Critical overview and outlook: pathogenesis, prevention, and treatment of hepatitis and hepatocarcinoma caused by hepatitis B virus.

    abstract::Viral hepatitis B is an enigmatic disease in which the host's own immune response to persistent viral infection may bring about host destruction through antiviral inflammatory responses which might otherwise present as a benign or inapparent disease. The simple solution to the hepatitis B problem is by immunoprophylax...


    pub_type: 杂志文章,评审


    authors: Hilleman MR

    更新日期:2003-12-01 00:00:00

  • Risk factors for pertussis hospitalizations in Australians aged 45 years and over: A population based nested case-control study.

    abstract::Although studies have described factors associated with pertussis hospitalization in children, data on adult hospitalization are sparse. We examined the association between patient characteristics and hospitalization among older adults with pertussis. We conducted a nested case-control study of participants in the 45 ...


    pub_type: 杂志文章


    authors: Karki S,McIntyre P,Newall AT,MacIntyre CR,Banks E,Liu B

    更新日期:2015-10-13 00:00:00

  • The V3 loop based multi-epitope polypeptide TAB9 adjuvated with montanide ISA720 is highly immunogenic in nonhuman primates and induces neutralizing antibodies against five HIV-1 isolates.

    abstract::In a previous work we selected montanide ISA720 (M-ISA720) among different adjuvants for the vaccination with a V3 loop based multi-epitope polypeptide TAB9. In this paper we present the evaluation of the toxicity and immunogenicity of this formulation in non-human primates. TAB9 in M-ISA720 was highly immunogenic in ...


    pub_type: 杂志文章


    authors: Gómez CE,Navea L,Lobaina L,Dubed M,Expósito N,Soto A,Duarte CA

    更新日期:1999-05-04 00:00:00

  • Advancing a vaccine to prevent hookworm disease and anemia.

    abstract::A human hookworm vaccine is under development and in clinical trials in Africa and the Americas. The vaccine contains the Na-APR-1 and Na-GST-1 antigens. It elicits neutralizing antibodies that interfere with establishment of the adult hookworm in the gut and the ability of the parasite to feed on blood. The vaccine t...


    pub_type: 杂志文章


    authors: Hotez PJ,Beaumier CM,Gillespie PM,Strych U,Hayward T,Bottazzi ME

    更新日期:2016-06-03 00:00:00

  • A meta-analysis of human papillomavirus type-distribution in women from South Asia: implications for vaccination.

    abstract:OBJECTIVE:To determine human papillomavirus (HPV) prevalence and type-distribution in women from South Asia, with and without cervical lesions, in order to estimate the impact of an HPV 16/18 prophylactic vaccine in this region and to assess additional types that should be incorporated in new vaccines. METHODS:A meta-...


    pub_type: 杂志文章,评审


    authors: Bhatla N,Lal N,Bao YP,Ng T,Qiao YL

    更新日期:2008-06-02 00:00:00

  • Characterization of pneumococcal meningitis before and after introduction of 13-valent pneumococcal conjugate vaccine in Niger, 2010-2018.

    abstract::Pneumococcal meningitis in the African meningitis belt is primarily caused by Streptococcus pneumoniae serotype 1, a serotype contained in the 13-valent pneumococcal conjugate vaccine (PCV13). In 2014, Niger introduced PCV13 with doses given at 6, 10, and 14 weeks of age. We leveraged existing meningitis surveillance ...


    pub_type: 杂志文章


    authors: Ousmane S,Kobayashi M,Seidou I,Issaka B,Sharpley S,Farrar JL,Whitney CG,Ouattara M

    更新日期:2020-05-13 00:00:00

  • The effect of HLA on immunological response to hepatitis B vaccine in healthy people: a meta-analysis.

    abstract:BACKGROUND AND AIM:Evidence is accumulating that several markers in the human leukocyte antigen (HLA) region have been associated with decreased or increased antibody response to hepatitis B vaccine in different individuals. This meta-analysis is to assess the associations of HLA class II DRB1 and DQB1 alleles with imm...


    pub_type: 杂志文章,meta分析


    authors: Li ZK,Nie JJ,Li J,Zhuang H

    更新日期:2013-09-13 00:00:00

  • Synergistic action of cholera toxin B subunit (and Escherichia coli heat-labile toxin B subunit) and a trace amount of cholera whole toxin as an adjuvant for nasal influenza vaccine.

    abstract::Cholera toxin B subunit (CTB) and Escherichia coli heat-labile toxin (LTB) (2 micrograms), each supplemented with a trace amount of cholera toxin (CT) (0.02-20 ng), were examined for the adjuvant effect on antibody (Ab) response against influenza inactivated HA (haemagglutinin) vaccine in Balb/c mice. Each mouse recei...


    pub_type: 杂志文章


    authors: Tamura S,Yamanaka A,Shimohara M,Tomita T,Komase K,Tsuda Y,Suzuki Y,Nagamine T,Kawahara K,Danbara H

    更新日期:1994-04-01 00:00:00

  • Awareness campaigns: experience in Mexico.

    abstract::The current total of AIDS cases in Mexico is 37,000 of which 86% have occurred in men. The major route of transmission is sexual. The campaign to prevent AIDS has fallen into four phases, and has now been extended to other sexually transmitted diseases, including hepatitis B. The first phase (1985-1989) was based arou...


    pub_type: 杂志文章


    authors: Hernández Tepichin G

    更新日期:2000-02-18 00:00:00

  • Intranasal vaccination of infant mice induces protective immunity in the absence of nasal-associated lymphoid tissue.

    abstract::Intranasal (i.n.) immunization is an effective regimen for the prophylaxis of respiratory diseases in early life. The aim of this study was to assess the need for nasal-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) in induction of protective immunity following mucosal vaccination of infant mice. We ...


    pub_type: 杂志文章


    authors: Sabirov A,Metzger DW

    更新日期:2008-03-17 00:00:00

  • Can the rolling cross-sectional survey design be used to estimate the effectiveness of influenza vaccines?

    abstract:INTRODUCTION:Observational studies of influenza vaccine effectiveness often study persons seeking medical care for acute respiratory infection (ARI). We conducted a pilot study to determine if vaccine effectiveness could be estimated in the general population with a novel rolling cross-sectional survey sampling design ...


    pub_type: 杂志文章


    authors: Donahue JG,Kieke BA,Szilagy PG,Blumkin AK,Hilgemann D,Flanders WD,Shay DK,Meece J,Gallivan S,Treanor J,Belongia EA

    更新日期:2014-11-12 00:00:00

  • Heterologous prime-boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus.

    abstract::The Middle East respiratory syndrome coronavirus (MERS-CoV) is a highly pathogenic and zoonotic virus with a fatality rate in humans of over 35%. Although several vaccine candidates have been developed, there is still no clinically available vaccine for MERS-CoV. In this study, we developed two types of MERS-CoV vacci...


    pub_type: 杂志文章


    authors: Jung SY,Kang KW,Lee EY,Seo DW,Kim HL,Kim H,Kwon T,Park HL,Kim H,Lee SM,Nam JH

    更新日期:2018-06-07 00:00:00

  • A population-based study on tetanus antitoxin levels in The Netherlands.

    abstract::We assessed the tetanus immunity of the general Dutch population and of religious groups refusing vaccination by means of population-based study to evaluate the effect of tetanus vaccination. More than 95% of those born after the introduction of routine vaccination had tetanus antitoxin levels above the minimum protec...


    pub_type: 杂志文章


    authors: de Melker HE,van den Hof S,Berbers GA,Nagelkerke NJ,Rümke HC,Conyn-van Spaendonck MA

    更新日期:1999-08-20 00:00:00

  • Enhancement of antibody response of turkeys to trivalent avian influenza vaccine by positively charged liposomal avridine adjuvant.

    abstract::Trivalent avian influenza (AIV) antigens (H4N8, H5N2 and H7N3), mixed with positively charged, negatively charged and neutral avridine-containing liposomes, and oil-emulsion were subcutaneously administered to 6-week-old turkeys. Charged liposomal avridine adjuvant, either positive or negative, produced a better antib...


    pub_type: 杂志文章


    authors: Fatunmbi OO,Newman JA,Sivanandan V,Halvorson DA

    更新日期:1992-01-01 00:00:00

  • Report of the 6th meeting on the evaluation of pandemic influenza vaccines in clinical trials World Health Organization, Geneva, Switzerland, 17-18 February 2010.

    abstract::On February 17-18, 2010, the World Health Organization (WHO) convened the 6th meeting on the "Evaluation of pandemic influenza vaccines in clinical trials" to review the progress made on new A (H1N1) 2009 vaccines and prototype H5N1 vaccines and their evaluation in clinical trials. A number of vaccine types were revie...


    pub_type: 杂志文章


    authors: Girard MP,Katz J,Pervikov Y,Palkonyay L,Kieny MP

    更新日期:2010-10-04 00:00:00

  • Characterization of the M protein and nucleoprotein genes of an avian influenza A virus which are involved in host range restriction in monkeys.

    abstract::A reassortant virus possessing RNA segment 7, which codes for the M1 and M2 proteins, of the avian influenza A/Mallard/New York/6750/78 (H2N2) virus and the other seven RNA segments of the human influenza A/Udorn/307/72 (H3N2) virus had been shown previously to be markedly restricted in replication in the respiratory ...


    pub_type: 杂志文章


    authors: Murphy BR,Buckler-White AJ,London WT,Snyder MH

    更新日期:1989-12-01 00:00:00

  • Identification of population at risk for future Clostridium difficile infection following hospital discharge to be targeted for vaccine trials.

    abstract:BACKGROUND:Efforts to develop a Clostridium difficile vaccine are underway; identification of patients at risk for C. difficile infection (CDI) is critical to inform vaccine trials. We identified groups at high risk of CDI ≥ 2 8 days after hospital discharge. METHODS:Hospital discharge data and pharmacy data from two ...


    pub_type: 杂志文章


    authors: Baggs J,Yousey-Hindes K,Ashley ED,Meek J,Dumyati G,Cohen J,Wise ME,McDonald LC,Lessa FC

    更新日期:2015-11-17 00:00:00

  • The genetic immunization with paraflagellar rod protein-2 fused to the HSP70 confers protection against late Trypanosoma cruzi infection.

    abstract::The immunological properties of the Trypanosoma cruzi paraflagellar rod proteins (PFR2 and PFR3) administered alone as well as fused to HSP70 have been analyzed in mice in the context of genetic immunization. The immunization of mice with the DNA vectors containing the PFRs gene or PFRs-HSP70 fused genes induced high ...


    pub_type: 杂志文章


    authors: Morell M,Thomas MC,Caballero T,Alonso C,López MC

    更新日期:2006-11-30 00:00:00

  • Persistence of protection through 33 months of age provided by immunization in infancy with two three-component acellular pertussis vaccines. Stage II Working Group.

    abstract::A large, randomized, placebo-controlled clinical trial in Italy on two three-component pertussis vaccines, given as DTaP in infancy, one manufactured by SmithKline and Beecham (SB) and one by Chiron Biocine (CB), found each vaccine to be 84% efficacious through the average age of 24 months. The cohort of children enro...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Salmaso S,Mastrantonio P,Wassilak SG,Giuliano M,Anemona A,Giammanco A,Tozzi AE,Ciofi degli Atti ML,Greco D

    更新日期:1998-08-01 00:00:00

  • Protection against an intranasal challenge by vaccines formulated with native and recombinant preparations of the Chlamydia trachomatis major outer membrane protein.

    abstract::To compare the ability of a native and a recombinant preparation of the major outer membrane protein of Chlamydia trachomatis mouse pneumonitis (MoPn; Ct-nMOMP and Ct-rMOMP) to protect against an intranasal (i.n.) challenge, BALB/c mice were vaccinated by the intramuscular (i.m.) and subcutaneous (s.c.) routes using C...


    pub_type: 杂志文章


    authors: Sun G,Pal S,Weiland J,Peterson EM,de la Maza LM

    更新日期:2009-08-06 00:00:00

  • Revisiting assumptions about age-based mixing representations in mathematical models of sexually transmitted infections.

    abstract:BACKGROUND:Sexual mixing between heterogeneous population subgroups is an integral component of mathematical models of sexually transmitted infections (STIs). This study compares the fit of different mixing representations to survey data and the impact of different mixing assumptions on the predicted benefits of hypoth...


    pub_type: 杂志文章


    authors: Easterly CW,Alarid-Escudero F,Enns EA,Kulasingam S

    更新日期:2018-09-05 00:00:00

  • Investigating adverse events following immunisation with pneumococcal polysaccharide vaccine using electronic General Practice data.

    abstract:BACKGROUND:In early 2011, following an increased number of reports of severe vaccine-related injection site reactions, Australian authorities recommended against administering repeat doses of the 23-valent pneumococcal polysaccharide vaccine (23vPPV) in otherwise healthy adults. The aim of this study was to assess a so...


    pub_type: 杂志文章


    authors: Trinh L,Macartney K,McIntyre P,Chiu C,Dey A,Menzies R

    更新日期:2017-03-13 00:00:00

  • Mucosal immunization with experimental feline immunodeficiency virus (FIV) vaccines induces both antibody and T cell responses but does not protect against rectal FIV challenge.

    abstract::Feline immunodeficiency virus (FIV) is a natural lentiviral pathogen of cats which can be experimentally transmitted via rectal and vaginal routes--the major routes of human immunodeficiency virus type 1 transmission in man. An important objective for lentiviral research is the development of vaccine strategies which ...


    pub_type: 杂志文章


    authors: Finerty S,Stokes CR,Gruffydd-Jones TJ,Hillman TJ,Reeves NA,Whiting CV,Schaaper WM,Dalsgaard K,Harbour DA

    更新日期:2000-08-01 00:00:00

  • Effect of storage temperatures on opacity and total nitrogen content of cholera vaccine.

    abstract::Fluid cholera vaccine was examined for its stability at 4-8 degrees C, 20-25 degrees C and 37 degrees C with regard to the number of organisms present and the total nitrogen content. Ten batches of fluid cholera vaccine manufactured at the Central Research Institute, Kasauli, India were taken for the study. The number...


    pub_type: 杂志文章


    authors: Gupta RK,Maheshwari SC,Sharma SB,Ahuja S,Saxena SN

    更新日期:1984-12-01 00:00:00

  • South Asia symposium on pneumococcal disease and the promise of vaccines - Meeting report.

    abstract::Despite the licensure of the pneumococcal conjugate vaccine (PCV) in the US and other Western countries for over 14 years, as of September 2014 only 4 South Asian countries were using PCV in their universal immunization program. To generate momentum toward addressing this issue a "South Asia symposium on pneumococcal ...


    pub_type: 杂志文章


    authors: Kumar R,Arora N,Santosham M

    更新日期:2016-05-17 00:00:00

  • Rectal immunization with antigen-containing microspheres induces stronger Th2 responses than oral immunization: a new method for vaccination.

    abstract::The rectum as an effective site for induction of systemic and local immunity has received little attention. Rectal immunization with microspheres-containing ovalbumin (MS-OVA) was tested for its ability to elicit systemic and mucosal immune responses. Rectal immunization with MS-OVA enhanced both Th2 dominant OVA-spec...


    pub_type: 杂志文章


    authors: Nakase H,Okazaki K,Tabata Y,Uchida K,Uose S,Ohana M,Nishi T,Watanabe T,Matsuura M,Hisatsune H,Matsumura K,Itoh T,Kawanami C,Chiba T

    更新日期:2001-11-12 00:00:00

  • Considerations in the design of vaccines that induce CD8 T cell mediated immunity.

    abstract::The protective capacity of many currently used vaccines is based on induction of neutralizing antibodies. Many pathogens, however, have adapted themselves in different ways to escape antibody-based immune protection. In particular, for those infections against which conventional neutralizing antibody-based vaccination...


    pub_type: 杂志文章,评审


    authors: Zaiss DM,Boog CJ,van Eden W,Sijts AJ

    更新日期:2010-11-16 00:00:00

  • Interpretation of primary care physicians' attitude regarding rotavirus immunisation using diffusion of innovation theories.

    abstract::To evaluate primary care physicians' attitude towards implementation of rotavirus (RV) immunisation into the Swiss immunisation schedule, an eight-question internet-based questionnaire was sent to the 3799 subscribers of InfoVac, a nationwide web-based expert network on immunisation issues, which reaches >95% of paedi...


    pub_type: 杂志文章


    authors: Agyeman P,Desgrandchamps D,Vaudaux B,Berger C,Diana A,Heininger U,Siegrist CA,Aebi C

    更新日期:2009-07-30 00:00:00

  • The history of the United States Advisory Committee on Immunization Practices (ACIP).

    abstract::The United States Advisory Committee on Immunization Practices (ACIP) is a federal advisory committee that develops written recommendations for use of vaccines licensed by the Food and Drug Administration (FDA) for the U.S. civilian population. Vaccine development and disease outbreaks contributed to the need for a sy...


    pub_type: 历史文章,杂志文章


    authors: Walton LR,Orenstein WA,Pickering LK

    更新日期:2015-01-09 00:00:00

  • Variable-number tandem repeat markers for identification of Brucella abortus 82 and 75/79-AV vaccine strains.

    abstract::In Russia, live vaccine strains Brucella abortus 82 and B. abortus 75/79-AV have been widely and extensively utilized for specific prophylaxis of cattle brucellosis. To differentiate these vaccine strains from each other and laboratory collection of other vaccine (n=4), reference (n=15) and field Brucella strains (n=6...


    pub_type: 杂志文章


    authors: Kulakov YK,Zheludkov MM,Sclyarov OD

    更新日期:2010-10-01 00:00:00