Abstract:
:Recombinant canarypox (CP) and fowlpox (FP) viruses that contained two forms of the HIV-1 (SF2 strain) env gene were engineered and their expression analysed in chick, simian and human cells. These vectors can efficiently replicate in avian but not in mammalian cells, in which infection is abortive. The two forms, consisting of the entire env open reading frame (IS+) or of the same gene lacking the putative immunosuppressive (IS-) region (amino acids 583-599), were individually inserted into the two virus vector backgrounds. In order to avoid premature transcription termination of the foreign gene and to improve protein expression, a mutagenesis was also performed within the T5NT motif without altering the amino acid sequence. By immunoprecipitation analyses, cells infected with CP and FP recombinants expressed HIV-1 env polypeptides of the appropriate molecular weight. We observed that the gp160 precursor was proteolytically cleaved except in MRC-5 cells infected with the IS- recombinants and that these polypeptides were glycosylated. Further analysis of these recombinant viruses by indirect immunofluorescence and syncytia inhibition assays indicated that the gp120 gp41 complex was present on the surface of infected cells, the number of syncytia being significantly lower when cells were infected by the CPIS- or FPIS- recombinants. Moreover, sera of immunized rabbits revealed the presence of specific antibodies in animals inoculated either with CP or with FP recombinants. These new constructs, which are unable to support a productive infection in human cells, might therefore also be a good anti-HIV-1 candidate vaccine in seropositive hosts.
journal_name
Vaccinejournal_title
Vaccineauthors
Radaelli A,De Giuli Morghen Cdoi
10.1016/0264-410x(94)90180-5subject
Has Abstractpub_date
1994-09-01 00:00:00pages
1101-9issue
12eissn
0264-410Xissn
1873-2518pii
0264-410X(94)90180-5journal_volume
12pub_type
杂志文章相关文献
VACCINE文献大全abstract::Chagas' disease is a major tropical disease for which a cure for chronic phase does not exist yet. Trypanosoma cruzi trans-sialidase (TS) seems to be involved in relevant processes such as infectivity, host survival and, very importantly, disease pathogenesis. In this study, we show that mice vaccinated with an engine...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.02.060
更新日期:2008-05-02 00:00:00
abstract::Leishmania donovani promastigote soluble antigens (sLAg) were encapsulated in non-phosphatidylcholine (non-PC) liposomes (escheriosomes) prepared from E. coli lipids. The escheriosome-based vaccine was investigated for its potential to elicit a protective immune response against experimental visceral leishmaniasis. Th...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.10.025
更新日期:2006-03-10 00:00:00
abstract::Tetanus and diphtheria (Td) antibodies were studied in HIV-1-infected women during puerperium. HIV group (n=61) was compared with Control group (n=101). Twenty-one women from HIV and 13 from Control group who had antibody levels lower than 0.1 IU/mL received a booster with Td vaccine. Antibodies were assessed by doubl...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.03.023
更新日期:2004-09-09 00:00:00
abstract::Influenza viruses exhibit a fascinating level of antigenic heterogeneity that facilitates re-infection in the human population. The human antibody repertoire also manifests endless capability for variation in the genes that specify the portion of antibody molecules interacting with epitopes. A recent explosion of tech...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.10.124
更新日期:2009-12-30 00:00:00
abstract::Annual vaccination rates among French health care workers (HCWs) are in decline even in Emergency Services to which patients at highest risk of influenza complications are admitted, and in which HCWs have the greatest risk of exposure to influenza from patients. We aimed here to identify knowledge and attitudes toward...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.11.086
更新日期:2017-01-05 00:00:00
abstract::ADVAX is a DNA-based candidate HIV vaccine that was safe but weakly immunogenic when delivered intramuscularly (IM) in humans. Studies were performed in animal models to determine whether an alternative delivery method, in vivo electroporation (EP), could improve the immunogenicity of ADVAX while maintaining an accept...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.11.011
更新日期:2011-01-17 00:00:00
abstract::Fluid cholera vaccine was examined for its stability at 4-8 degrees C, 20-25 degrees C and 37 degrees C with regard to the number of organisms present and the total nitrogen content. Ten batches of fluid cholera vaccine manufactured at the Central Research Institute, Kasauli, India were taken for the study. The number...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(84)90046-x
更新日期:1984-12-01 00:00:00
abstract::Japanese encephalitis is a major cause of encephalitis in Asia. Cases occur largely in rural areas of the South and East Asian region resulting in significant morbidity and mortality. Multiple vaccines exist to control Japanese encephalitis, but all suffer from problems. Envelope protein domain III of Japanese encepha...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.09.006
更新日期:2009-11-16 00:00:00
abstract::We investigated the influence of the site of administration of DNA vaccine on the induced immune response. DNA vaccines were administered by electroporation at three different sites: tibial cranial muscle, abdominal skin and ear pinna. Aiming to draw general conclusions about DNA vaccine delivery, we successively used...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.05.005
更新日期:2015-06-22 00:00:00
abstract::Rotavirus causes approximately 450,000 deaths annually among children less than 5 years of age worldwide, almost half of which occur in Africa. After the recent completion of successful trials of 2 new rotavirus vaccines, the World Health Organization has recommended these vaccines for all children worldwide. Because ...
journal_title:Vaccine
pub_type:
doi:10.1016/j.vaccine.2011.10.004
更新日期:2012-04-27 00:00:00
abstract::Immunization with Mycobacterium bovis Bacille Calmette-Guerin (BCG) did not induce adequate Th1 responses to the latency antigen, HspX of M. tuberculosis. To increase the immunogenicity and protective efficacy of BCG, a recombinant BCG strain over-expressing antigen HspX (rBCG::X) was constructed. The recombinant stra...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.05.063
更新日期:2010-07-19 00:00:00
abstract::In Portugal, the 7-valent pneumococcal conjugate vaccine (PCV7) was not introduced in the national immunization plan but was commercially available between 2001 and 2010. We studied serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae carried by children in 2009 and 2010. Vaccination with PC...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.02.045
更新日期:2016-03-29 00:00:00
abstract:BACKGROUND:To evaluate the potential public health impact of the live attenuated tetravalent Sanofi Pasteur dengue vaccine (CYD-TDV) we analyzed data from the reported clinical trials to calculate vaccine preventable disease incidence (VPDI) and number needed to vaccinate (NNV) based on the licensure indication for per...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.03.017
更新日期:2016-04-29 00:00:00
abstract::Plasmid DNA encoding herpes simplex virus type-1 glycoprotein D (gD-1) was complexed with asialoorosomucoid conjugated to poly-L-lysine. Following its intravenous injection into BALB/c mice, this complex was targeted to the liver. Liver cells expressing gD-1 were detected immunohistochemically through day 6 post-immun...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(99)00438-7
更新日期:2000-02-14 00:00:00
abstract:BACKGROUND:In 2018/19, English NHS trusts (NHSTs) implemented an 'opt-out' policy for seasonal flu vaccination in frontline healthcare workers (HCWs). HCWs declining the vaccination were asked to sign an opt-out form and provide a reason for their decision. In addition, HCWs working in higher risk hospital environments...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.04.026
更新日期:2020-05-27 00:00:00
abstract::The aim of this study was to characterize the adverse events of attenuated measles vaccine in mainland China. We carried out prospective follow-up of adverse events after mass measles immunization practices, categorizing them into two groups: allergic reactions and serious adverse events. The incidence of anaphylaxis ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.10.106
更新日期:2011-04-18 00:00:00
abstract::Successful launches of recently licensed vaccines contrast with pharmaceutical industry concerns about unfavorable market conditions, making the status and future of vaccine development uncertain. We assessed trends in private-sector vaccine research and development for the period 1995-2008, using a global pharmaceuti...
journal_title:Vaccine
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.vaccine.2009.11.007
更新日期:2010-02-03 00:00:00
abstract::Targeting immune checkpoint receptors has emerged as an effective strategy to induce immune-mediated cancer regression in the subset of patients who have significant pre-existing anti-tumor immunity. For the remainder, effective anti tumor responses may require vaccination. Radiotherapy, traditionally used to achieve ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2015.05.105
更新日期:2015-12-16 00:00:00
abstract::Cervical cancer that is associated with high-risk human papillomaviruses (HPV) is the most common malignancy in Indian women. Therefore, the establishment of a prevention program is urgently required considering both vaccination and screening. However, relatively little is known about the prevalence of the different H...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2008.11.041
更新日期:2009-01-29 00:00:00
abstract::Vector-directed lymphokine expression represents a novel approach to the attenuation of live recombinant viruses which might be used as vaccines. Expression of interleukin-2 (IL-2) by recombinant vaccinia virus has been shown to significantly attenuate virus virulence in rodent species without diminishing immunogenici...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(90)90171-h
更新日期:1990-02-01 00:00:00
abstract::General vaccination with the 7-valent pneumococcal conjugate vaccine was recommended in Germany in July 2006 for all children <2 years. The proportion of reported invasive pneumococcal disease (IPD) caused by vaccine serotypes before vaccine introduction was considerably lower than in the US. We report data from natio...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.04.057
更新日期:2009-06-24 00:00:00
abstract::A novel triterpenoidal saponin, called pulcherrimasaponin (CP05), isolated from the leaves of Calliandra pulcherrima Benth. shows remarkable similarities to the previously described potent adjuvant, QS21 saponin (Quillaja saponaria Molina). On the basis of chemical and physicochemical evidence, its structure was estab...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.08.022
更新日期:2005-01-11 00:00:00
abstract::Malaria caused by Plasmodium falciparum continues to threaten millions of people living in the tropical parts of the world. A vaccine that confers sterile and life-long protection remains elusive despite more than 30years of effort and resources invested in solving this problem. Antibodies to a malaria vaccine candida...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.05.070
更新日期:2017-07-05 00:00:00
abstract:OBJECTIVES:To assess the effectiveness and cost-effectiveness of routine childhood vaccination by new vaccines against rotavirus in France. METHODS:We constructed a Markov decision tree to compare two alternatives: "no vaccination" and "vaccination". A hypothetical birth cohort of 750,000 children was followed until 3...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.11.064
更新日期:2008-01-30 00:00:00
abstract::The objective of this paper is to review adaptive immunity of young animals using examples from my own experience and from the literature. Trials carried out by us with a modified live and inactivated canine parvovirus vaccine in newborn puppies provide evidence of the immune capacity of these puppies. With regard to ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/s0264-410x(98)00110-8
更新日期:1998-08-01 00:00:00
abstract::We described here the characterization of Schistosoma mansoni egg antigens recognized by human monoclonal antibodies B10 (HmAb-B10) and D5 (HmAb-D5). SDS-PAGE and Western blot analysis revealed that these monoclonals recognized two antigens of M W 44.7/56.8 kDa, with pI of 7.0 and 7.8, respectively. The passive transf...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(96)00305-2
更新日期:1997-06-01 00:00:00
abstract::A capture IgE assay in EIA with diphtheria toxin and tetanus toxoid as model systems was developed and evaluated in relation to RIA in a DT booster study. The EIA and the RIA gave very similar results, although the EIA determined IgE over a wider range of concentrations, without interference by other subclasses. The c...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(97)00274-0
更新日期:1998-05-01 00:00:00
abstract::Vaccines have drastically reduced the mortality and morbidity of many diseases. However, vaccines have historically been developed empirically, and recent development of vaccines against current pandemics such as HIV and malaria has been met with difficulty. The advent of high-throughput technologies, coupled with sys...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2015.03.072
更新日期:2015-09-29 00:00:00
abstract::The potency assay currently used to evaluate consistency of manufacture for the anthrax vaccine is contingent upon meeting specified parameters after statistical analysis of the percent survival and time to death of vaccinated guinea pigs after challenge with spores of a virulent strain of Bacillus anthracis. During t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2003.12.027
更新日期:2004-07-29 00:00:00
abstract:BACKGROUND:Several chronic disease states have been identified as pneumococcal vaccination indications due to their ability to increase pneumococcal disease development and subsequent mortality. However, the risk of mortality according to the number of these disease states present is unknown. We sought to determine the...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.02.026
更新日期:2017-03-23 00:00:00