Immunization with recombinant paraflagellar rod protein induces protective immunity against Trypanosoma cruzi infection.


:In the present study, we have produced recombinant paraflagellar rod proteins (PFR) and report their use for successful vaccination of mice against Trypanosoma cruzi. This protection is associated with a highly polarized type 1 cytokine production profile. Additionally, we have analyzed the gene sequence encoding PFR-2 to determine the degree of conservation among seven highly diverse strains of T. cruzi, and found it to be highly conserved. The results presented here indicate that the PFR antigens are highly conserved, and immunization with rPFR-1, PFR-2, or an equimolar mix of the PFR-1, -2, and -3 proteins provides protective immunity against Trypanosoma cruzi.






Luhrs KA,Fouts DL,Manning JE




Has Abstract


2003-06-20 00:00:00
















  • Distribution and genetic variability of three vaccine components in a panel of strains representative of the diversity of serogroup B meningococcus.

    abstract::With the aim of studying the molecular diversity of the antigens of a new recombinant vaccine against meningococcus serogroup B, the three genes coding for the main vaccine components GNA (Genome-derived Neisseria Antigen) 1870 (fHbp, factor H Binding Protein), GNA1994 (NadA, Neisseria adhesin A) and GNA2132 were sequ...


    pub_type: 杂志文章


    authors: Bambini S,Muzzi A,Olcen P,Rappuoli R,Pizza M,Comanducci M

    更新日期:2009-05-11 00:00:00

  • Considerations for using ETEC and Shigella disease burden estimates to guide vaccine development strategy.

    abstract::Enterotoxigenic E. coli (ETEC) and Shigella are enteropathogens causing significant global morbidity and mortality, particularly in low-income countries. No licensed vaccine exists for either pathogen, but candidates are in development, with the most advanced candidates potentially approaching pivotal efficacy testing...


    pub_type: 杂志文章,评审


    authors: Hosangadi D,Smith PG,Giersing BK

    更新日期:2019-11-28 00:00:00

  • Early disappearance of maternal anti-measles, mumps, rubella, and varicella antibodies in Indian infants.

    abstract:BACKGROUND:Immunization of children with vaccines against Measles, Mumps, Rubella, and Varicella (MMRV) is practiced globally with varied recommendations. In India, measles vaccine is administered alone or as MMR at 9 months age. Varicella vaccine is not routinely used. Immunization age is a function of disappearance o...


    pub_type: 杂志文章


    authors: Malshe N,Palkar S,Kulkarni R,Lalwani S,Mishra AC,Arankalle V

    更新日期:2019-03-07 00:00:00

  • Prophylactic evaluation of recombinant extracellular superoxide dismutase of Brugia malayi in jird model.

    abstract::The immunoscreening of Brugia malayi adult cDNA library with pooled endemic normal sera identified several seroreactive clones including, EC-SOD which contained a 612 bp insert and showed significant nucleotide and deduced amino acid sequence homologies with superoxide dismutase (SOD) of other nematode parasites. The ...


    pub_type: 杂志文章


    authors: Dabir S,Dabir P,Goswami K,Reddy MV

    更新日期:2008-07-04 00:00:00

  • Evidence supporting the hypothesis that specifically modifying a malaria peptide to fit into HLA-DRbeta1*03 molecules induces antibody production and protection.

    abstract::EBA-175 protein is used as ligand in Plasmodium falciparum binding to erythrocytes. Evidence shows that conserved peptide 1815 from this protein having high red blood cell binding ability plays an important role in the invasion process. This peptide is neither immunogenic nor protective. Residues were substituted by a...


    pub_type: 杂志文章


    authors: Cifuentes G,Salazar LM,Vargas LE,Parra CA,Vanegas M,Cortes J,Patarroyo ME

    更新日期:2005-02-18 00:00:00

  • Antibodies to Plasmodium vivax transmission-blocking vaccine candidate antigens Pvs25 and Pvs28 do not show synergism.

    abstract::Transmission-blocking vaccines against malaria parasites target molecules expressed by sexual stage parasites to elicit antibodies that prevent the infection of the mosquito vector. Pvs25 and Pvs28, expressed on the surface of ookinetes, are potential candidates for such a vaccine and induce antibodies that block the ...


    pub_type: 杂志文章


    authors: Hisaeda H,Collins WE,Saul A,Stowers AW

    更新日期:2001-12-12 00:00:00

  • From current vaccine recommendations to everyday practices: An analysis in five sub-Saharan African countries.

    abstract:BACKGROUND:Estimates of WHO and UNICEF vaccination coverage may provide little insight into the extent to which vaccinations are administered on time. Yet, lack of adherence to the recommended age to receive a specific vaccination may have detrimental health consequences. For example, delays in receiving vaccination wi...


    pub_type: 杂志文章


    authors: Delrieu I,Gessner BD,Baril L,Roset Bahmanyar E

    更新日期:2015-12-16 00:00:00

  • Two-dose schedules for human papillomavirus vaccine: Systematic review and meta-analysis.

    abstract::Simpler schedules for human papillomavirus (HPV) vaccine delivery could improve vaccine coverage and the effectiveness of cervical cancer prevention. The objective of this study was to systematically review evidence about the effects of two-dose compared with three-dose schedules for human papillomavirus (HPV) vaccine...


    pub_type: 杂志文章,meta分析,评审


    authors: D'Addario M,Redmond S,Scott P,Egli-Gany D,Riveros-Balta AX,Henao Restrepo AM,Low N

    更新日期:2017-05-19 00:00:00

  • Modeling the cost-effectiveness of maternal acellular pertussis immunization (aP) in different socioeconomic settings: A dynamic transmission model of pertussis in three Brazilian states.

    abstract:OBJECTIVES:Using dynamic transmission models we evaluated the health and cost outcomes of adding acellular pertussis (aP) vaccination of pregnant women to infant vaccination in three Brazilian states that represent different socioeconomic conditions. The primary objective was to determine whether the same model structu...


    pub_type: 杂志文章


    authors: Luz PM,Struchiner CJ,Kim SY,Minamisava R,Andrade ALS,Sanderson C,Russell LB,Toscano CM

    更新日期:2021-01-03 00:00:00

  • Immunogenicity and reactogenicity of the Biken acellular pertussis vaccine in young adults.

    abstract::To assess the reactogenicity and immunogenicity of the Biken acellular pertussis vaccine (Pa) following administration of a single vaccine dose to young adults with or without a history of prior pertussis immunization, 104 healthy, male and female adults without primary pertussis immunization were enrolled in Mainz (f...


    pub_type: 杂志文章


    authors: Schmitt HJ,Mohnike K,Zepp F,Herden P,Hosbach P

    更新日期:2000-10-15 00:00:00

  • Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1.

    abstract::Mucin1 (MUC1) is expressed ubiquitously on breast cancer cells and is a potential target for the generation of cytotoxic T cells for vaccination against breast cancer. Thus far studies of the immunogenicity of MUC1 have used peptides from the variable number of tandem repeat (VNTR); mice so immunised can generate stro...


    pub_type: 杂志文章


    authors: Pietersz GA,Li W,Osinski C,Apostolopoulos V,McKenzie IF

    更新日期:2000-04-03 00:00:00

  • Evaluation of measles vaccination programme conducted in two separate health centres.

    abstract::Measles vaccination programmes at two vaccination centres in Ibadan, Nigeria were evaluated using the following factors as indicators: type of vaccination centre, age at vaccination, titre of vaccine, economic, health and social status of vaccinee. There was a significant association between type of vaccination centre...


    pub_type: 临床试验,杂志文章


    authors: Onoja AL,Adu FD,Tomori O

    更新日期:1992-01-01 00:00:00

  • Streptococcus pneumoniae serotypes in Utah adults at the end of the PCV7 era.

    abstract::While heptavalent pneumococcal conjugate vaccine (PCV) has decreased vaccine type invasive pneumococcal disease (IPD) nationwide, rapid serotype replacement and increasing parapneumonic empyema, has been reported in Utah children. The effect of pediatric vaccination on adults in this population is unknown. We identifi...


    pub_type: 杂志文章


    authors: Kendall BA,Dascomb KK,Mehta RR,Mason EO,Ampofo K,Pombo DJ,Pavia AT,Byington CL

    更新日期:2011-11-15 00:00:00

  • Immunization with the recombinant antigen Ss-IR induces protective immunity to infection with Strongyloides stercoralis in mice.

    abstract::Human intestinal infections with the nematode Strongyloides stercoralis remain a significant problem worldwide and a vaccine would be a useful addition to the tools available to prevent and control this infection. The goal of this study was to test single antigens for their efficacy in a vaccine against S. stercoralis...


    pub_type: 杂志文章


    authors: Abraham D,Hess JA,Mejia R,Nolan TJ,Lok JB,Lustigman S,Nutman TB

    更新日期:2011-10-19 00:00:00

  • Partial efficacy of a VSV-SIV/MVA-SIV vaccine regimen against oral SIV challenge in infant macaques.

    abstract::Despite antiretroviral medications, the rate of pediatric HIV-1 infections through breast-milk transmission has been staggering in developing countries. Therefore, the development of a vaccine to protect vulnerable infant populations should be actively pursued. We previously demonstrated that oral immunization of newb...


    pub_type: 杂志文章


    authors: Marthas ML,Van Rompay KK,Abbott Z,Earl P,Buonocore-Buzzelli L,Moss B,Rose NF,Rose JK,Kozlowski PA,Abel K

    更新日期:2011-04-12 00:00:00

  • Pandemic whole-virion, Vero-cell-derived, adjuvant-free influenza A H1N1 vaccine in patients with solid tumors and hematologic malignancies receiving concurrent anticancer treatment: Immunogenicity, tolerability, and acceptability during the pandemic situ

    abstract::Patients with malignancies are considered to be at increased risk of acquiring influenza. Because of higher complication and case fatality rates, preventive measures such as vaccination are of great interest. The objective of this study was to assess the acceptability, tolerability and immunogenicity of an adjuvant-fr...


    pub_type: 杂志文章


    authors: Lagler H,Tobudic S,Ramharter M,Elandt K,Sperr WR,Redlberger-Fritz M,Popow-Kraupp T,Jäger U,Zielinski CC,Burgmann H

    更新日期:2012-11-06 00:00:00

  • Changes in serotype distribution of Haemophilus influenzae meningitis isolates identified through laboratory-based surveillance following routine childhood vaccination against H. influenzae type b in Brazil.

    abstract::Following routine childhood vaccination against Haemophilus influenzae type b (Hib) disease in Brazil in 1999, passive laboratory surveillance reported increasing numbers of non-b serotypes and nontypeable H. influenzae (NTHi) from meningitis cases. To characterize this increase, we analyzed data on 3910 H. influenzae...


    pub_type: 杂志文章


    authors: Zanella RC,Bokermann S,Andrade AL,Flannery B,Brandileone MC

    更新日期:2011-11-08 00:00:00

  • Poliovirus seroprevalence before and after interruption of poliovirus transmission in Kano State, Nigeria.

    abstract:INTRODUCTION:In September 2015, Nigeria was removed from the list of polio-endemic countries after more than 12months had passed since the detection of last wild poliovirus case in the country on 24 July 2014. We are presenting here a report of two polio seroprevalence surveys conducted in September 2013 and October 20...


    pub_type: 杂志文章


    authors: Iliyasu Z,Verma H,Craig KT,Nwaze E,Ahmad-Shehu A,Jibir BW,Gwarzo GD,Gajida AU,Weldon WC,Steven Oberste M,Takane M,Mkanda P,Muhammad AJG,Sutter RW

    更新日期:2016-09-30 00:00:00

  • A novel peptide isolated from phage library to substitute a complex system for a vaccine against staphylococci infection.

    abstract::Staphylococcus aureus is a major human pathogen and many of the strains are resistant to conventional antibacterial treatment. The bacteria cause disease largely due to the production of multiple toxins, whose synthesis is controlled by an RNA molecule termed RNAIII. The production of RNAIII is induced by quorum sensi...


    pub_type: 杂志文章


    authors: Yang G,Gao Y,Dong J,Xue Y,Fan M,Shen B,Liu C,Shao N

    更新日期:2006-02-20 00:00:00

  • An ESAT-6:CFP10 DNA vaccine administered in conjunction with Mycobacterium bovis BCG confers protection to cattle challenged with virulent M. bovis.

    abstract::The potency of genetic immunization observed in the mouse has demonstrated the utility of DNA vaccines to induce cell-mediated and humoral immune responses. However, it has been relatively difficult to generate comparable responses in non-rodent species. The use of molecular adjuvants may increase the magnitude of the...


    pub_type: 杂志文章,随机对照试验


    authors: Maue AC,Waters WR,Palmer MV,Nonnecke BJ,Minion FC,Brown WC,Norimine J,Foote MR,Scherer CF,Estes DM

    更新日期:2007-06-11 00:00:00

  • Identification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans.

    abstract::Toxoplasma gondii is an intracellular parasite that causes severe neurologic and ocular disease in immune-compromised and congenitally infected individuals. There is no vaccine protective against human toxoplasmosis. Herein, immunization of L(d) mice with HF10 (HPGSVNEFDF) with palmitic acid moieties or a monophosphor...


    pub_type: 杂志文章


    authors: Tan TG,Mui E,Cong H,Witola WH,Montpetit A,Muench SP,Sidney J,Alexander J,Sette A,Grigg ME,Maewal A,McLeod R

    更新日期:2010-05-21 00:00:00

  • Th2-polarisation of cellular immune memory to neonatal pertussis vaccination.

    abstract::Current infant vaccination against pertussis in North America and Australia requires three doses of vaccines including diphtheria, tetanus and acellular pertussis antigens (DTaP) at 2, 4 and 6 months of age. Interest is growing in the possibility that vaccination at birth might provide earlier protection of infants, b...


    pub_type: 杂志文章


    authors: White OJ,Rowe J,Richmond P,Marshall H,McIntyre P,Wood N,Holt PG

    更新日期:2010-03-19 00:00:00

  • Ultraviolet-C irradiation for inactivation of viruses in foetal bovine serum.

    abstract::Foetal Bovine Serum (FBS) and porcine trypsin are one of the essential raw materials used in the manufacturing of cell culture based viral vaccines. Being from animal origin, these raw materials can potentially contaminate the final product by known or unknown adventitious agents. The issue is more serious in case of ...


    pub_type: 杂志文章


    authors: Vaidya V,Dhere R,Agnihotri S,Muley R,Patil S,Pawar A

    更新日期:2018-07-05 00:00:00

  • Protection against influenza virus infection by nasal vaccination in advance of sublethal irradiation.

    abstract::The effect of sublethal gamma-ray irradiation on the protection conferred by a nasal influenza vaccine was investigated in BALB/c mice. A radiation dose of 7 Gy was selected as the sublethal dose as this caused exacerbation of the influenza but was not lethal in the mouse model. Mice were irradiated 7 days before, on ...


    pub_type: 杂志文章


    authors: Hasegawa H,Kadowaki S,Takahashi H,Iwasaki T,Tamura S,Kurata T

    更新日期:2000-05-22 00:00:00

  • Knowledge, attitude and awareness among healthcare professionals about influenza vaccination in Peshawar, Pakistan.

    abstract::A cross-sectional study was carried out among HCPs in Northwest General Hospital & Research Centre, Hayatabad Peshawar, Pakistan. The purpose of this study was to investigate knowledge, awareness and attitude of HCPs towards influenza vaccination. A total of N=170 questionnaires were distributed among the staff. There...


    pub_type: 杂志文章


    authors: Khan TM,Khan AU,Ali I,Wu DB

    更新日期:2016-03-08 00:00:00

  • Haemolytic activities and adjuvant effect of Astragalus membranaceus saponins (AMS) on the immune responses to ovalbumin in mice.

    abstract::In this study, the haemolytic activities of Astragalus membranaceus saponins (AMS) and its adjuvant potentials on the cellular and humoral immune responses of ICR mice against OVA were evaluated. We determined the haemolytic activity of AMS using 0.5% rabbit red blood cell. AMS showed a slight haemolytic effect, with ...


    pub_type: 杂志文章


    authors: Yang ZG,Sun HX,Fang WH

    更新日期:2005-10-25 00:00:00

  • A response to Fu et al.'s "Educational interventions to increase HPV vaccination acceptance".

    abstract::This commentary is a response to a systematic review, recently published in Vaccine, which investigates the effectiveness of educational interventions in increasing uptake of the human papillomavirus vaccine. The systematic review by Fu et al. (2014) enhances the field's understanding of human papillomavirus vaccine i...


    pub_type: 评论,杂志文章


    authors: Shapiro GK,Joyal-Desmarais K,Perez S,Rosberger Z

    更新日期:2014-11-12 00:00:00

  • A therapeutic HIV-1 vaccine reduces markers of systemic immune activation and latent infection in patients under highly active antiretroviral therapy.

    abstract::HIV infection is characterized by chronic immune activation and the establishment of a pool of latently infected cells. Antiretroviral therapy (ART) can suppress viral load to undetectable levels in peripheral blood by standard measure, however immune activation/chronic inflammation and latent infection persist and af...


    pub_type: 杂志文章


    authors: Pallikkuth S,Bolivar H,Fletcher MA,Babic DZ,De Armas LR,Gupta S,Termini JM,Arheart KL,Stevenson M,Tung FY,Fischl MA,Pahwa S,Stone GW

    更新日期:2020-06-02 00:00:00

  • Childhood vaccinations: Hidden impact of COVID-19 on children in Singapore.

    abstract::Although the direct health impact of Coronavirus disease (COVID-19) pandemic on child health is low, there are indirect impacts across many aspects. We compare childhood vaccine uptake in three types of healthcare facilities in Singapore - public primary care clinics, a hospital paediatric unit, and private paediatric...


    pub_type: 杂志文章


    authors: Zhong Y,Clapham HE,Aishworiya R,Chua YX,Mathews J,Ong M,Wang J,Murugasu B,Chiang WC,Lee BW,Chin HL

    更新日期:2021-01-29 00:00:00

  • Enhanced mucosal and systemic immune response with intranasal immunization of mice with HIV peptides entrapped in PLG microparticles in combination with Ulex Europaeus-I lectin as M cell target.

    abstract::The predominant route of HIV infection is through the sexual transmission via M cells. Most of the peptide and protein vaccines show poor transport across the epithelial barrier and are commonly administered by parenteral route. In the present study four HIV peptides from envelope (gp 41-LZ (leucine zipper), gp 41-FD ...


    pub_type: 杂志文章


    authors: Manocha M,Pal PC,Chitralekha KT,Thomas BE,Tripathi V,Gupta SD,Paranjape R,Kulkarni S,Rao DN

    更新日期:2005-12-01 00:00:00