Abstract:
:MHC class II molecules associate with peptides in the endocytic pathway. Different endosomal locations for peptide loading of class II molecules, varying from early endosomes (EE) to lysosomes, have been assigned on the basis of subcellular fractionation experiments. We have determined the intracellular location of HLA-DM, a molecule that supports peptide loading of class II molecules, by separating vesicles from the melanoma cell line Mel JuSo on the basis of buoying density and surface charge. In both fractionations, HLA-DM co-fractionated with a lysosomal compartment containing beta-hexosaminidase (beta-hex) activity and not with endosomes. Further analysis showed that HLA-DM mainly co-fractionated with a sub-lysosomal structure characterized by a relative low density and containing both pro- and mature cathepsin D and MHC class II molecules. Fluid phase markers first enter this compartment before entering high-density lysosomes that contain exclusively mature cathepsin D, some HLA-DM and no detectable MC class II molecules. Finally we determined the intracellular location of neutral and acidic peptidases. Whereas neutral peptidase activity was detected in the endoplasmic reticulum and/or plasma membrane fractions, acidic peptidase activity exclusively migrated at the position of HLA-DM containing lysosomal vesicles. Our results show that class II molecules co-migrate with HLA-DM, pro- and mature cathepsin D, beta-hex and acidic peptidase activity. HLA-DM, cathepsin d and class II molecules were not observed at the position of EE. Our data suggest that HLA-DM-mediated peptide loading of class II molecules occurs in a lysosomal subcompartment.
journal_name
Int Immunoljournal_title
International immunologyauthors
Fernandez-Borja M,Verwoerd D,Sanderson F,Aerts H,Trowsdale J,Tulp A,Neefjes Jdoi
10.1093/intimm/8.5.625subject
Has Abstractpub_date
1996-05-01 00:00:00pages
625-40issue
5eissn
0953-8178issn
1460-2377journal_volume
8pub_type
杂志文章abstract::p40 was previously described as a regulatory molecule capable of inhibiting both the natural and the CD16-mediated cytotoxicity of NK cells. In this study, we analyze the effect of p40 molecule engagement on the NK cell triggering induced by activating HLA class I-specific NK receptors (NKR) or on TCR alpha beta-media...
journal_title:International immunology
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abstract::Inadequate co-stimulation of tumor reactive T cells may contribute to the fact that antigenic tumors are not normally rejected by the immune system. We recently reported that the induction of profound unresponsiveness in a T cell clone by melanoma cells expressing MHC class II antigens may provide a mechanism for thes...
journal_title:International immunology
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abstract::It is now generally recognized that bone marrow is the survival niche for antigen-specific plasma cells with long-term immunological memory. These cells release antibodies into the circulation, needed to prime effector cells in the secondary immune response. These antibodies participate in the surveillance for antigen...
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pub_type: 杂志文章,评审
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abstract::Oral administration of large doses of protein antigen generally induces a state of systemic unresponsiveness currently termed mucosally induced tolerance. In this study, we used human milk protein (HMP) without casein as a multi-protein antigen for the study of mucosally induced tolerance. The HMP utilized in this stu...
journal_title:International immunology
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更新日期:1998-04-01 00:00:00
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journal_title:International immunology
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abstract::Antibody responses to ingested antigens can be inhibited by a mechanism known as oral tolerance which acts to prevent excessive stimulation from luminal contents. Local IgA responses can be induced in this non-responsive environment and during intestinal inflammation, mucosal IgG responses can also be increased. The p...
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pub_type: 杂志文章
doi:10.1093/intimm/dxt062
更新日期:2014-05-01 00:00:00
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更新日期:1994-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg006
更新日期:2003-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.4.395
更新日期:1991-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/dxm109
更新日期:2007-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/11.4.569
更新日期:1999-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.10.1585
更新日期:1994-10-01 00:00:00
abstract::BALB.xid mice, carrying an X-linked mutation leading to the absence of CD5+ B cells, are highly resistant to Trypanosoma cruzi infection. These mice clear blood parasites in the acute phase of infection and do not develop the inflammatory infiltration characteristically observed in the chronic phase of susceptible str...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.8.1213
更新日期:1996-08-01 00:00:00
abstract::IL-7 is a required factor for T-cell homeostasis. Because of low expression levels and poor reagent availability, the cellular sources of IL-7 have proven challenging to characterize. In this study, we describe a reporter mouse in which enhanced GFP is expressed from the endogenous Il7 locus. We show that IL-7 is prod...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxt012
更新日期:2013-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.1.57
更新日期:2000-01-01 00:00:00
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更新日期:2007-06-01 00:00:00
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更新日期:2010-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.9.1323
更新日期:1994-09-01 00:00:00
abstract::CD9 is a member of the tetraspanin superfamily which is characterized by four transmembrane (TM) domains and associates with other surface molecules. This tetraspanin was recently found to be expressed on mature T cells. Here, we investigated which molecules associate with CD9 on T cells and which CD9 domains are requ...
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pub_type: 杂志文章
doi:10.1093/intimm/11.12.2043
更新日期:1999-12-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn023
更新日期:2008-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm079
更新日期:2007-09-01 00:00:00
abstract::Human intestinal intraepithelial lymphocytes (iIEL) are a unique population of predominantly CD8 alpha beta+, TCR alpha beta+ lymphocytes and, to a lesser extent, TCP gamma delta+ lymphocytes that proliferate poorly to anti-CD3 mitogenic signals but display significant cytolytic activity. Studies in mouse model system...
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pub_type: 杂志文章,评审
doi:10.1093/intimm/7.8.1237
更新日期:1995-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/12.11.1605
更新日期:2000-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxaa017
更新日期:2020-06-26 00:00:00
abstract::BALB/c mice are susceptible to cutaneous leishmaniasis upon infection with Leishmania major while C57BL/6 are not. There is a major promastigote surface protease (PSP or gp63) which is available in both native and recombinant forms, and for which the primary amino acid sequence is known. Immunization with PSP has been...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.5.785
更新日期:1994-05-01 00:00:00