Abstract:
:Soluble recombinant (r) P-selectin and rP-selectin immobilized on plastic surfaces were tested for their capacity to activate neutrophils to produce superoxide anion. Soluble rP-selectin was incapable of activating leukocytes, whereas immobilized rP-selectin was able to induce leukocyte activation. When neutrophils were pretreated with a low dose of IL-8, granulocyte colony stimulating factor or granulocyte macrophage colony stimulating factor, soluble rP-selectin was no longer inert. These cytokine-primed leukocytes produced superoxide anion in the presence of soluble rP-selectin. During this priming period, sialyl Lewis X (sLe(X)) epitopes redistributed to one end of the leukocytes. Similar polarization of sLe(X) epitopes was observed at the attachment site of cells that adhered to immobilized rP-selectin. Cap formation and superoxide anion production induced by solid-phase P-selectin or by IL-8 and soluble rP-selectin treatment were inhibited by treatment of the leukocytes with cytochalasin B. These observations suggest that the redistribution of the carbohydrate ligands and the polarization of the leukocyte surface through an active process is a prerequisite but not sufficient to leukocyte superoxide production through P-selectin.
journal_name
Int Immunoljournal_title
International immunologyauthors
Nagata K,Tsuji T,Matsushima K,Hanai N,Irimura Tdoi
10.1093/intimm/12.4.487subject
Has Abstractpub_date
2000-04-01 00:00:00pages
487-92issue
4eissn
0953-8178issn
1460-2377journal_volume
12pub_type
杂志文章abstract::EBV-encoded latent membrane protein 1 (LMP1) is critical for EBV-driven B-cell transformation and most EBV-associated malignancies and is also implicated in exacerbation of autoimmunity. LMP1 functionally mimics the TNFR superfamily member CD40, but LMP1-induced signals and downstream B-cell functions are amplified an...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxt052
更新日期:2014-03-01 00:00:00
abstract::We have evaluated the capacity of a novel, nanoparticle-based tumor vaccine to eradicate established tumors in mice. C57BL/6 mice were intradermally (i.d.) inoculated with ovalbumin (OVA)-expressing EG-7 tumor cells. When the tumor size reached 7-8 mm, the tumor-bearing mice were i.d. injected near the tumor-draining ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh381
更新日期:2006-03-01 00:00:00
abstract::T-cell immune responses are critical for protection of the host and for disease pathogenesis during infection with Plasmodium species. We examined the regulation of CD4(+) T-cell cytokine responses during infection with Plasmodium berghei ANKA (PbA). CD4(+) T cells from PbA-infected mice produced IFN-γ, IL-4 and IL-10...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq448
更新日期:2010-12-01 00:00:00
abstract::Factors responsible for the predictable generation of Th1 or Th2 immune responses to microorganisms in vivo are not well characterized, although the ability of antigen presenting cells (APC) to provide co-stimulation, the kinetics of MHC-peptide ligand generation as well as the cytokine environment are all considered ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.4.481
更新日期:1999-04-01 00:00:00
abstract::Lymphokine activity in seven myelin basic protein (MBP)-specific T cell clones was examined. All of the clones recognize MBP peptide 1-9 in the context of I-Au. A strong positive correlation was found between levels of lymphotoxin (LT) and tumor necrosis factor alpha (TNF-alpha) mRNA and biological activity on L929 ce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.6.539
更新日期:1990-01-01 00:00:00
abstract::Structural and functional aspects of the accessory molecule lymphocyte function associated antigen (LFA)-3 (CD58) have been examined following the transfection of DAP.3 and P815 cells with a cDNA clone encoding the glycosyl phosphatidylinositol (GPI)-linked form of human LFA-3. Despite earlier observations that DAP.3 ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.6.673
更新日期:1992-06-01 00:00:00
abstract::The endocytic and phagocytic activities of a population of MHC IIhi CD11c+ dendritic cell (DC)-like cells in synovium-rich tissues (SRTs) of normal rat paws were compared with CD163+ cells (putative macrophages) from the same tissues and pseudo-afferent lymph DCs, peritoneal macrophages and blood monocytes. Fifty perc...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh291
更新日期:2005-08-01 00:00:00
abstract::Seeligeriolysin O (LSO), a member of cholesterol-dependent cytolysins of Listeria seeligeri, exhibits cytokine-inducing activity. In this study, we examined the profile of cytokines expressed in macrophages of mice after stimulation with full-length form of recombinant LSO (rLSO530), C-terminal-truncated protein (rLSO...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh341
更新日期:2005-12-01 00:00:00
abstract::Immunopathology and immune responses to Schistosoma mansoni were examined in IL-4 -/- mice. IL-5 and IL-10 production by lymphoid cells stimulated with soluble egg antigen (SEA), peripheral eosinophilia and serum levels of soluble IL-4 receptor but not IgE were all significantly elevated over background normal levels ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.4.435
更新日期:1996-04-01 00:00:00
abstract::In this paper we describe a monoclonal antibody, JR9-318, which was obtained from a wild-derived mouse immunized with the M14T cell line (CD3+CD4-CD8-) in order to describe antigenic molecules expressed during T cell differentiation. This mAb precipitated a protein from the M14T cell line or subclones that migrates li...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.6.699
更新日期:1992-06-01 00:00:00
abstract::SKG mice, a newly established model of rheumatoid arthritis (RA), spontaneously develop autoimmune arthritis accompanying extra-articular manifestations, such as interstitial pneumonitis. To examine possible roles of T cells for mediating this systemic autoimmunity, we generated T cell clones from arthritic joints of ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn091
更新日期:2008-10-01 00:00:00
abstract::HIV-1 infection in CD4(+) T cells initiates a viral cytopathic effect (CPE) that is dependent on the activation of intracellular protein tyrosine kinases (PTK). PTK in T cells are also activated during the course of TCR or CD4 receptor engagement and the manner of receptor engagement may generate signals leading eithe...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.1.65
更新日期:1996-01-01 00:00:00
abstract::The anti-CD20 chimeric monoclonal antibody rituximab mediates cytotoxicity in malignant B cells via multiple mechanisms, including complement-dependent cytotoxicity (CDC), antibody-dependent cellular cytotoxicity (ADCC) and direct induction of apoptosis. To optimize treatment of non-Hodgkin's lymphoma, a fuller unders...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs048
更新日期:2012-08-01 00:00:00
abstract::Although there has been extensive analysis on the capacity of MHC-peptide tetramers to bind antigen-specific TCR, there have been comparatively few studies regarding the role of the CD4 and CD8 co-receptors in binding and activation by these multimeric molecules. Here, we start from the observation that different anti...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.1.39
更新日期:2002-01-01 00:00:00
abstract::The association of celiac disease (CD) with HLA-DQ2 and HLA-DQ8 is indicative of preferential mucosal T cell recognition of gluten fragments bound to either DQ allele. We have recently identified two gluten-derived, HLA-DQ8-restricted T cell stimulatory peptides, one each from gliadin and glutenin, recognized by speci...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.8.1157
更新日期:2000-08-01 00:00:00
abstract::Fas (CD95) is a cell surface death receptor belonging to the tumor necrosis factor receptor superfamily, which mediates apoptosis-inducing signaling when activated by Fas ligand or its agonistic antibody. lpr mice with a loss of apoptosis-inducing function mutation in the Fas gene develop systemic autoimmune disease a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs109
更新日期:2013-05-01 00:00:00
abstract::Analysis of immunoglobulin expression in mice transgenic for either a kappa light chain (driven by the kappa enhancer) or lambda light chain (driven by the IgH enhancer) revealed that the transgenic light chains are expressed by the majority of B lymphocytes in the neonatal mice. However, the proportion of B cells tha...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.5.509
更新日期:1989-01-01 00:00:00
abstract::Lipid mediators, such as prostanoids and leukotrienes (LTs), exert a range of actions through their own receptors on cell surfaces in various pathophysiological conditions. It has been reported that the production of prostanoids and LTs is significantly elevated in the skin lesions of some chronic inflammatory skin di...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxy087
更新日期:2019-08-23 00:00:00
abstract::The aim of this in vitro study was to evaluate if osteoclasts (OCs) and dendritic cells (DCs), both of monocyte origin, can support the survival of normal human plasma cells (PCs). PCs differentiate from plasmablasts (PBs) arising from activated B cells, essentially memory B cells. To study the survival of both PBs (C...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn035
更新日期:2008-06-01 00:00:00
abstract::Unlike splenic B cells from normal BALB/c mice, those from autoimmune-prone NZB x NZW (B/W) F1 young mice were hyperresponsive to interleukin 2 (IL2) and proliferated even in the absence of prior stimulation signals. We found that the B cell subpopulation responsible for this event belongs to that with 6B2 (B220)-dull...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.1.99
更新日期:1989-01-01 00:00:00
abstract::Under equilibrium conditions, the affinities of five anti-IgG2a mAb isolated from virus-infected mice were comparable to other high-affinity auto-antibodies. Similar to rheumatoid factors, these anti-IgG2a auto-antibodies bound to aggregated or complexed IgG2a with 50 to 1500-fold higher avidity than their monomeric c...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.7.1125
更新日期:1995-07-01 00:00:00
abstract::The two-signal model states that activation of naive T cells requires a signal 1 stimulus through the TCR and a co-stimulatory signal 2. By contrast, signal 1 alone is sufficient for pre-activated T cells. Recently, however, it has been shown that under certain conditions T cells can bypass the requirement for co-stim...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.5.619
更新日期:1998-05-01 00:00:00
abstract::Mice lacking the IL-2 receptor beta chain (IL-2R beta) exhibit an autoimmune reaction characterized by generalized T cell activation, production of autoantibodies, myeloproliferation and severe anemia. T cells of IL-2R beta-/- mice were examined to elucidate the mechanism responsible for their abnormal activation and ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.9.1367
更新日期:1997-09-01 00:00:00
abstract::BALB.xid mice, carrying an X-linked mutation leading to the absence of CD5+ B cells, are highly resistant to Trypanosoma cruzi infection. These mice clear blood parasites in the acute phase of infection and do not develop the inflammatory infiltration characteristically observed in the chronic phase of susceptible str...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.8.1213
更新日期:1996-08-01 00:00:00
abstract::Germline CH transcripts initiate from a non-coding I exon and terminate downstream of the associated CH exons. Ig heavy chain class switch recombination from the VDJ-C mu gene to particular downstream CH genes appears to be regulated by a process that involves mitogen and/or cytokine induction of germline CH transcrip...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.4.491
更新日期:1994-04-01 00:00:00
abstract::Spectroscopic methods have been applied to elucidate conformational differences responsible for the immunological diversity of two synthetic multichain copolymers, Tyr1Tyr2Glu3Glu4-poly-DL-Ala--poly-Lys and Tyr1Glu2Tyr3Glu4-poly-DL-Ala--poly-Lys. Despite their far-reaching structural similarity in the epitope peptide ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.6.591
更新日期:1993-06-01 00:00:00
abstract::Insulitis develops in non-obese diabetic (NOD) mice as a multicentric and asynchronous process. In an effort to understand how this T cell mediated process expands within each islet and propagates between the islets of the same pancreas, we have analyzed the junctional diversity of TCR V beta 6 and V beta 8.2 transcri...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.1.139
更新日期:1995-01-01 00:00:00
abstract::In vaccine development, a major objective is to induce strong, specific T cell responses. This might be obtained by targeting antigen to cell surface molecules that efficiently channel the antigen into endocytic compartments for loading of MHC molecules. Antibodies have been used to deliver antigen; however, it is imp...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf110
更新日期:2002-12-01 00:00:00
abstract::Previously we examined the ability of the host's immune responses to regulate Ig production in an IgE-secreting murine plasma cell tumor (B53). In the present study we have examined the reverse phenomenon, in that we have investigated the effects of this and other plasma cell tumors on the immune responses of their ho...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.7.1029
更新日期:1995-07-01 00:00:00
abstract::MHC class II molecules associate with peptides in the endocytic pathway. Different endosomal locations for peptide loading of class II molecules, varying from early endosomes (EE) to lysosomes, have been assigned on the basis of subcellular fractionation experiments. We have determined the intracellular location of HL...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.5.625
更新日期:1996-05-01 00:00:00