Abstract:
:Structural and functional aspects of the accessory molecule lymphocyte function associated antigen (LFA)-3 (CD58) have been examined following the transfection of DAP.3 and P815 cells with a cDNA clone encoding the glycosyl phosphatidylinositol (GPI)-linked form of human LFA-3. Despite earlier observations that DAP.3 cells are deficient in GPI anchoring LFA-3 was expressed efficiently on DAP.3, as well as on P815 cells. Immunoprecipitation of LFA-3 from 35S-labelled cells revealed that the molecule expressed on the DAP.3 cells had a molecular weight intermediate between the transmembrane and GPI-linked forms expressed by human B cells. This suggests that the DAP.3 cells have a default pathway whereby the RNA transcript which encodes the GPI-linked form of the molecule can also encode an integral membrane protein. Functionally, expression of LFA-3 by DAP.3 which had previously been transfected with the genes encoding HLA-DR1 led to a marked augmentation of the proliferative response of five out of eight anti-DR1 human T cell clones. This effect was not reproduced when DR1 and LFA-3 were expressed by separate populations of DAP.3 cells, suggesting that the ligands for CD2 and for the T cell's receptor must be expressed on the same cell membrane. Expression of human LFA-3 also led to a substantial increase in the proliferative response of human peripheral blood T cells to a DR alloantigen. Separation of T cells into CD45RO+ and CD45RO- populations revealed that the augmentation was more marked for the memory than the virgin population. The mechanisms responsible for these differences are discussed.
journal_name
Int Immunoljournal_title
International immunologyauthors
Greenlaw R,Robinson P,Heaton T,Lombardi G,Lechler Rdoi
10.1093/intimm/4.6.673subject
Has Abstractpub_date
1992-06-01 00:00:00pages
673-80issue
6eissn
0953-8178issn
1460-2377journal_volume
4pub_type
杂志文章abstract::Using a complete series of overlapping peptides, we have identified the T cell epitopes of a malaria vaccine candidate, the circumsporozoite (CS) protein, that are recognized by sporozoite-exposed residents of a non-endemic country. This protein and subunits from it are being considered as malaria sporozoite vaccine c...
journal_title:International immunology
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更新日期:2000-03-01 00:00:00
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更新日期:1995-11-01 00:00:00
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doi:10.1093/intimm/8.6.807
更新日期:1996-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/dxn080
更新日期:2008-09-01 00:00:00
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更新日期:2003-01-01 00:00:00
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