Abstract:
:bcl-2 transgenic mice develop anti-double-stranded (ds) DNA antibodies similar to those present in systemic lupus erythematosus. To begin to understand where a breakdown in the regulation of autoreactive lymphocytes is occurring, we have used a bcl-2 transgene (Tg) in conjunction with an Ig Tg that allows us to identify and track anti-dsDNA B cells. Previously, we have shown that anti-dsDNA B cells are actively tolerized in BALB/c mice as manifested by their developmental arrest, follicular exclusion, increased in vivo turnover rate and lack of their antibody in the serum. The bcl-2 Tg mice increased the lifespan of anti-dsDNA B cells, but did not alter the other features of tolerance, indicating that the anergy of the anti-dsDNA B cells is independent of their reduced lifespan. Furthermore, these data suggest that the serum anti-dsDNA antibodies in bcl-2 transgenic mice are not due to a breakdown in the induction or maintenance of B cell anergy; rather they may originate from B cells that have transited through a germinal center.
journal_name
Int Immunoljournal_title
International immunologyauthors
Mandik-Nayak L,Nayak S,Sokol C,Eaton-Bassiri A,Madaio MP,Caton AJ,Erikson Jdoi
10.1093/intimm/12.3.353subject
Has Abstractpub_date
2000-03-01 00:00:00pages
353-64issue
3eissn
0953-8178issn
1460-2377journal_volume
12pub_type
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