Abstract:
:Experiments in transgenic mice have demonstrated that thymocyte differentiation into the mature CD4+ helper or CD8+ cytotoxic T cell lineage is ultimately dependent upon the specificity of the TCR for class II or class I MHC molecules respectively. However, the initial mechanistic events involved in this process remain unclear. To address this issue, we have expressed a TCR specific for an ovalbumin peptide and the Kb class I MHC molecule in the DPK CD4+CD8+ precursor T cell line. This cell line originally expressed a TCR specific for a pigeon cytochrome c peptide and class II MHC molecules and has been shown previously to differentiate into CD4+CD8- cells upon recognition of antigen in vitro or thymic epithelial cells in vivo. Surprisingly, we find that recognition of either class I or class II MHC by these cells initiates differentiation into the CD4+ lineage and induces down-regulation of recombination associated genes. Unlike recognition of class II MHC, however, recognition of class I MHC does not induce full maturation. These results support a model in which (i) commitment to the CD4 lineage occurs prior to positive selection and (ii) CD4 lineage commitment is associated with a requirement for activation by a class II MHC-specific TCR in order to complete differentiation.
journal_name
Int Immunoljournal_title
International immunologyauthors
DeKoning J,Carbone FR,Kaye Jdoi
10.1093/intimm/7.4.541subject
Has Abstractpub_date
1995-04-01 00:00:00pages
541-9issue
4eissn
0953-8178issn
1460-2377journal_volume
7pub_type
杂志文章abstract::The endocytic and phagocytic activities of a population of MHC IIhi CD11c+ dendritic cell (DC)-like cells in synovium-rich tissues (SRTs) of normal rat paws were compared with CD163+ cells (putative macrophages) from the same tissues and pseudo-afferent lymph DCs, peritoneal macrophages and blood monocytes. Fifty perc...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh291
更新日期:2005-08-01 00:00:00
abstract::The ability of Bcl-2 to inhibit apoptotic cell death is well established. Several homologues of the bcl-2 gene, such as bax, bcl-x or mcl-1, have recently been identified. Like Bcl-2, both Bcl-XL and Mcl-1 appear to function as repressors of apoptotic cell death, whereas Bax facilitates it, indicating possible interac...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.11.1817
更新日期:1995-11-01 00:00:00
abstract::The B cell-activating factor from the tumor necrosis factor family (BAFF) is an important regulator of B cell immunity. Recently, we demonstrated that recombinant BAFF also provides a co-stimulatory signal to T cells. Here, we studied expression of BAFF in peripheral blood leukocytes and correlated this expression wit...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh043
更新日期:2004-03-01 00:00:00
abstract::Candida albicans is a commensal fungus that predominantly resides on mucosal surfaces and can cause lethal systemic infection when the host defense is compromised. Candidalysin is a cytolytic peptide toxin produced by C. albicans hyphae that is essential for mucosal tissue damage and is believed to contribute to the e...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxaa070
更新日期:2020-10-10 00:00:00
abstract::In this study, we explored the potential of human naive CD4(+) T cells to acquire regulatory properties upon stimulation. We demonstrated that, in vitro, pre-activated naive CD4(+)CD25(-)CD45RA(+) T cells could become anergic and suppressive CD4(+)CD25(+) T cells upon lower intensity TCR stimulation. These CD4(+)CD25(...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp068
更新日期:2009-09-01 00:00:00
abstract::To develop a comprehensive catalogue of phenotypic and functional parameters of human CD4(+) T cell differentiation stages, we have performed microarray gene expression profiling on subpopulations of human thymocytes and circulating naive CD4(+) T cells, including CD3(-)CD4(+)CD8(-) intrathymic T progenitor cells, CD3...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh112
更新日期:2004-08-01 00:00:00
abstract::We have found that FOXP3 is an oligomeric component of a large supramolecular complex. Certain FOXP3 mutants with single amino acid deletions in the leucine zipper domain of FOXP3 are associated with the X-linked autoimmunity-allergic dysregulation (XLAAD) and immunodysregulation, polyendocrinopathy and enteropathy, X...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm043
更新日期:2007-07-01 00:00:00
abstract::Constitutive expression of p35 and p40 IL-12 mRNA was detected in splenic macrophages isolated from aged mice. Macrophages were also implicated as the cell type responsible for the dysregulated IL-6 and tumor necrosis factor (TNF)-alpha commonly observed to be constitutively produced by lymphoid cells from aged donors...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.5.745
更新日期:1997-05-01 00:00:00
abstract::The dynamic behaviour of isolated populations of peritoneal LPS-reactive (LPSr) B lymphocytes was studied upon transfer of peritoneal cells (PerC) from C57BL/6 LPS responder into C57BL/10ScCr LPS non-responder mice. We have followed the persistence and life-span of the transferred LPSr donor B cells in the spleen and ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.1.73
更新日期:1990-01-01 00:00:00
abstract::Induction of mucosal tolerance by inhalation of soluble peptides with defined T cell epitopes is receiving much attention as a means of specifically down-regulating pathogenic T cell reactivities in autoimmune and allergic disorders. Experimental autoimmune encephalomyelitis (EAE) induced in the Lewis rat by immunizat...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.8.1139
更新日期:1998-08-01 00:00:00
abstract::The integrins have been grouped into three subfamilies based on the utilization of three distinct beta chain subunits, beta 1, beta 2, and beta 3. The recent discovery of beta 4 and beta 5 subunits, and the sharing of alpha subunits between beta subfamilies reflects a greater structural and functional diversity of the...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.11.1097
更新日期:1990-01-01 00:00:00
abstract::M cells are responsible for uptake of mucosal antigens in Peyer's patches (PPs). Differentiation of M cells is thought to be induced by interactions between follicle-associated epithelium and PP cells; however, it remains elusive what types of immune cells function as M-cell inducers. Here, we attempted to identify th...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq478
更新日期:2011-04-01 00:00:00
abstract::Previously we observed elevation of the serum concentration of two acute-phase protein (AFP) complement components (C9 and C1-inhibitor) in patients with chronic hepatitis C who responded (R) to IFN-alpha therapy, but not in non-responders (NR). In the present study we investigated the effect of high-dose IFN-alpha th...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh024
更新日期:2004-01-01 00:00:00
abstract::Analyses of bone marrow (BM) lymphocytes in C57BL/6 mice homozygous for the lpr mutation (B6.lpr) disclosed low numbers of pre-B and B cells, as compared with age-matched control B6 mice. BM depletion in B6.lpr mice was selective for B-lineage cells, appeared in young adults, and developed markedly with age and diseas...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.2.247
更新日期:1996-02-01 00:00:00
abstract::Plasmid DNA vectors have been constructed with minigenes encoding a single cytotoxic T lymphocyte (CTL) epitope from either the M2 protein of respiratory syncytial virus (RSV) or from the nucleoprotein of measles virus (MV) with or without a signal sequence (also called secretory or leader sequence). Following intrade...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.10.1441
更新日期:1998-10-01 00:00:00
abstract::The intestinal epithelium not only acts as the physical structure that separates the intestinal lumen from the body but also actively participates in intestinal barrier functions. In the past decade, significant progress has been made in the development of culture technologies to maintain intestinal epithelial cells (...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxy065
更新日期:2019-02-06 00:00:00
abstract::Antibody-secreting cells (ASCs) in respiratory tract tissues provide a first line of defense against invading pathogens. These cells often secrete IgA that is efficiently transcytosed across epithelial barriers into the airway lumen where pathogens can be blocked at their point of entry. Previous literature has report...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxw035
更新日期:2016-11-01 00:00:00
abstract::The MHC class I antigens enhance the T cell response to various mitogenic stimuli. Class I antigens co-cap and associate with the CD3 structure on these cells. The present work shows that co-aggregation of these MHC antigens with CD3 induces a sustained elevated Ca2+ response in T cells. The duration required for a 50...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.3.481
更新日期:1994-03-01 00:00:00
abstract::Natural killer (NK) cells are a major FcγRIIIA-expressing lymphocyte population that mediate antibody-dependent cellular cytotoxicity. Although NK cells are critical for immunity against viruses and tumors, they are also activated in the joints of patients with rheumatoid arthritis (RA) and may be involved in disease ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz010
更新日期:2019-04-26 00:00:00
abstract::Sequestration of neutrophils and release of histotoxic mediators are considered important for the development of pathologic alterations of the lung defined as adult respiratory distress syndrome. Mechanisms of inflammatory lung injury caused by abdominal sepsis were investigated using the colon ascendens stent periton...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.2.217
更新日期:1999-02-01 00:00:00
abstract::Heat shock protein (Hsp) 60 is thought to function as endogenous danger signal by activating professional antigen-presenting cells (APC) through toll-like receptor (TLR) 4 and CD14, a mechanism that is also used by bacterial LPS. We recently showed that Hsp60 binds LPS and enhances LPS-induced immune stimulation. On t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn003
更新日期:2008-03-01 00:00:00
abstract::P-selectin is a Ca(2+)-dependent lectin that participates in leukocyte adhesion to vascular endothelium and platelets. Myeloid cells and a subset of T lymphocytes express carbohydrate ligands at the cell surface. Previously, we suggested that heat stable antigen (HSA/mouse CD24), an extensively glycosylated cell surfa...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.10.1557
更新日期:1995-10-01 00:00:00
abstract::The efficacy of cytotoxic T lymphocytes (CTL) has been shown to be highly dependent upon their functional avidity (the sensitivity of their cellular response to MHC-peptide complexes). To examine this relationship, we employed target cell lysis as a quantitative measure and established a set of four CTL clones that ex...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.6.817
更新日期:2001-06-01 00:00:00
abstract::Monoclonal non-specific suppressor factor (MNSF), a lymphokine produced by a murine hybridoma, was originally found to inhibit the generation of lipopolysaccharide (LPS)-induced Ig-secreting cells. Most recently, we demonstrated the recombinant form of the ubiquitin-like segment (rUbi-L) or MNSF beta, an isoform of MN...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.11.1659
更新日期:1996-11-01 00:00:00
abstract::pp56lck is a member of the src family of tyrosine kinases mainly expressed in T lymphocytes. Src tyrosine kinases have been implicated in the control of cell growth and differentiation in different cell types, but the mechanism of regulation of these enzymes is poorly understood. In order to characterize the distinct ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.7.673
更新日期:1991-07-01 00:00:00
abstract::Intracellular signaling triggered by the antigen receptor of primary B-1 (CD5+ B) lymphocytes is insufficient to bring about cell cycle progression to S phase; this appears to result from a block in signal propagation prior to the activation of protein kinase C. The present studies were undertaken to evaluate specific...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.7.1011
更新日期:1994-07-01 00:00:00
abstract::Lymphokine activity in seven myelin basic protein (MBP)-specific T cell clones was examined. All of the clones recognize MBP peptide 1-9 in the context of I-Au. A strong positive correlation was found between levels of lymphotoxin (LT) and tumor necrosis factor alpha (TNF-alpha) mRNA and biological activity on L929 ce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.6.539
更新日期:1990-01-01 00:00:00
abstract::Malaria infection induces the production of serum antibodies to a variety of malaria antigens but the prevalence of antibodies to any particular antigen is typically much less than 100%. It has been assumed that non-responsiveness to defined antigens in malaria immune subjects is due to HLS-mediated restriction of the...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.6.905
更新日期:1996-06-01 00:00:00
abstract::Lactic acid bacteria (LAB) are abundant in the gastrointestinal tract where they continuously regulate the immune system. NK cells are potently activated by dendritic cells (DCs) matured by inflammatory stimuli, and NK cells are present in the gut epithelium and in mesenteric lymph nodes, but it is not known how NK-DC...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm103
更新日期:2007-12-01 00:00:00
abstract::It has proven difficult to evaluate the functional potential of germinal center (GC) B cells, including those from Peyer's patches (PP), by either in vivo or in vitro methods. Thus, rather than assess secreted Ig product as an indicator of functional potential we have instead sought to detect mRNAs related to the vari...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.12.1253
更新日期:1991-12-01 00:00:00