Immunoblot analysis of cellular expression of Bcl-2 family proteins, Bcl-2, Bax, Bcl-X and Mcl-1, in human peripheral blood and lymphoid tissues.


:The ability of Bcl-2 to inhibit apoptotic cell death is well established. Several homologues of the bcl-2 gene, such as bax, bcl-x or mcl-1, have recently been identified. Like Bcl-2, both Bcl-XL and Mcl-1 appear to function as repressors of apoptotic cell death, whereas Bax facilitates it, indicating possible interactions among them in the control of cellular survival. To investigate the in vivo role of expression of bcl-2 gene family products, immunoblot analysis using corresponding specific antisera was performed for peripheral blood cells and some lymphoid tissues in humans. We demonstrated that all Bcl-2 family proteins were expressed at various levels in hematolymphoid cell subpopulations isolated from peripheral blood, tonsil, spleen and thymus. Lymphoid expression of Bcl-2 family proteins tended to increase following activation, but declined with time in culture. Loss of Bcl-2 in cultured lymphoid cells was especially marked. Sole expression of Bax, but not other members of the Bcl-2 family, was observed on neutrophils, seemingly reflecting their shortest life-span among blood leukocytes. The results support the notion that a balance of expression of Bcl-2 family proteins may regulate the life and death of hematolymphoid cells at different stages of cell differentiation and activation.


Int Immunol


International immunology


Ohta K,Iwai K,Kasahara Y,Taniguchi N,Krajewski S,Reed JC,Miyawaki T




Has Abstract


1995-11-01 00:00:00












  • Construction and binding analysis of recombinant single-chain TCR derived from tumor-infiltrating lymphocytes and a cytotoxic T lymphocyte clone directed against MAGE-1.

    abstract::The TCR is responsible for the specificity of cytotoxic T lymphocytes (CTL) by recognizing peptides presented in the context of MHC. By producing recombinant soluble TCR, it is possible to study this interaction at the molecular level. We generated single-chain TCR (scTCR) from tumor infiltrating lymphocytes (TIL) and...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Lake DF,Salgaller ML,van der Bruggen P,Bernstein RM,Marchalonis JJ

    更新日期:1999-05-01 00:00:00

  • Tyk2 deficiency protects joints against destruction in anti-type II collagen antibody-induced arthritis in mice.

    abstract::Tyrosine kinase-2 (Tyk2) participates in the signaling pathways of multiple cytokines in innate and acquired immunity. In the present study, we investigated the in vivo involvement of Tyk2 in anti-type II collagen antibody-induced arthritis (CAIA) using Tyk2-deficient mice. Hind paws of wild-type mice showed massive s...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Ishizaki M,Muromoto R,Akimoto T,Ohshiro Y,Takahashi M,Sekine Y,Maeda H,Shimoda K,Oritani K,Matsuda T

    更新日期:2011-09-01 00:00:00

  • Expression of multilectin receptors and comparative FITC-dextran uptake by human dendritic cells.

    abstract::Dendritic cells (DC) are potent antigen-presenting cells and understanding their mechanisms of antigen uptake is important for loading DC with antigen for immunotherapy. The multilectin receptors, DEC-205 and macrophage mannose receptor (MMR), are potential antigen-uptake receptors; therefore, we examined their expres...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Kato M,Neil TK,Fearnley DB,McLellan AD,Vuckovic S,Hart DN

    更新日期:2000-11-01 00:00:00

  • Natural peptide ligand motifs of two HLA molecules associated with myasthenia gravis.

    abstract::The peptide motifs of two HLA molecules, B8 and DR3(17), which are associated with autoimmune diseases including myasthenia gravis, were determined from natural peptide pools using Edman degradation. The majority of HLA-B8 ligands are nonamers preferentially terminated by leucine. As a characteristic feature of the HL...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Malcherek G,Falk K,Rötzschke O,Rammensee HG,Stevanović S,Gnau V,Jung G,Melms A

    更新日期:1993-10-01 00:00:00

  • Longitudinal analysis of T cells responding to tetanus toxoid in healthy subjects as well as in pediatric patients after bone marrow transplantation: the identification of identical TCR-CDR3 regions in time suggests long-term stability of at least part of

    abstract::To understand the nature of long-term Th immune responses, we investigated in the present study the TCRBV gene repertoire of CD4(+) T cells specific for the recall antigen tetanus toxoid (TT) in recipients of an allogeneic bone marrow transplantation (allo-BMT) at several time points after transplantation and in their...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Godthelp BC,van Tol MJ,Vossen JM,van den Elsen PJ

    更新日期:2001-04-01 00:00:00

  • Transfection of HLA-DR-expressing DAP.3 cells with a cDNA clone encoding the glycosyl phosphatidylinositol-linked form of lymphocyte function associated antigen-3: biochemical features and functional consequences.

    abstract::Structural and functional aspects of the accessory molecule lymphocyte function associated antigen (LFA)-3 (CD58) have been examined following the transfection of DAP.3 and P815 cells with a cDNA clone encoding the glycosyl phosphatidylinositol (GPI)-linked form of human LFA-3. Despite earlier observations that DAP.3 ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Greenlaw R,Robinson P,Heaton T,Lombardi G,Lechler R

    更新日期:1992-06-01 00:00:00

  • Three distinctive steps in Peyer's patch formation of murine embryo.

    abstract::Investigation of the process of Peyer's patch (PP) formation has been hampered by difficulties in identifying its initial step in the embryo. In this study, we overcame this problem by means of whole-mount immunohistochemistry using mAb against the molecules which were expressed in the cells accumulated at the site of...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Adachi S,Yoshida H,Kataoka H,Nishikawa S

    更新日期:1997-04-01 00:00:00

  • Anti-Semaphorin 3A neutralization monoclonal antibody prevents sepsis development in lipopolysaccharide-treated mice.

    abstract::Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A bot...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Yamashita N,Jitsuki-Takahashi A,Ogawara M,Ohkubo W,Araki T,Hotta C,Tamura T,Hashimoto S,Yabuki T,Tsuji T,Sasakura Y,Okumura H,Takaiwa A,Koyama C,Murakami K,Goshima Y

    更新日期:2015-09-01 00:00:00

  • PI3K is a negative regulator of IgE production.

    abstract::The production of IgE, a main player in allergic disorders such as asthma and atopic dermatitis, is strictly regulated and the serum concentrations of IgE are normally kept at a much lower level than other isotypes. We found that mice deficient for the p85alpha regulatory subunit of class IA phosphoinositide 3-kinase ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Doi T,Obayashi K,Kadowaki T,Fujii H,Koyasu S

    更新日期:2008-04-01 00:00:00

  • Mucolipin 1 positively regulates TLR7 responses in dendritic cells by facilitating RNA transportation to lysosomes.

    abstract::Toll-like receptor 7 (TLR7) and TLR9 sense microbial single-stranded RNA (ssRNA) and ssDNA in endolysosomes. Nucleic acid (NA)-sensing in endolysosomes is thought to be important for avoiding TLR7/9 responses to self-derived NAs. Aberrant self-derived NA transportation to endolysosomes predisposes to autoimmune diseas...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Li X,Saitoh S,Shibata T,Tanimura N,Fukui R,Miyake K

    更新日期:2015-02-01 00:00:00

  • Fas deficiency in mice with the Balb/c background induces blepharitis with allergic inflammation and hyper-IgE production in conjunction with severe autoimmune disease.

    abstract::Fas (CD95) is a cell surface death receptor belonging to the tumor necrosis factor receptor superfamily, which mediates apoptosis-inducing signaling when activated by Fas ligand or its agonistic antibody. lpr mice with a loss of apoptosis-inducing function mutation in the Fas gene develop systemic autoimmune disease a...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Takahashi S,Futatsugi-Yumikura S,Fukuoka A,Yoshimoto T,Nakanishi K,Yonehara S

    更新日期:2013-05-01 00:00:00

  • NADPH diaphorase staining suggests a transient and localized contribution of nitric oxide to host defence against an intracellular pathogen in situ.

    abstract::Nitric oxide (NO) is formed constitutively in neurons by the constitutive enzyme NO synthase (cNOS) and acts as a neurotransmitter. It has already been shown that cNOS-containing neurons are identical to neurons staining for NADPH diaphorase and vice versa. Effector cells of the immune response produce high NO levels ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Flesch IE,Hess JH,Kaufmann SH

    更新日期:1994-11-01 00:00:00

  • The exploitation of distinct recognition receptors in dendritic cells determines the full range of host immune relationships with Candida albicans.

    abstract::Dendritic cells (DC) sense saprophytic yeast and pathogenic, filamentous forms of Candida albicans in a specific way, resulting in disparate patterns of DC and T(h) cell activation. Using human and murine DC, such disparate patterns could be traced to the exploitation of distinct recognition receptors. Although usage ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Romani L,Montagnoli C,Bozza S,Perruccio K,Spreca A,Allavena P,Verbeek S,Calderone RA,Bistoni F,Puccetti P

    更新日期:2004-01-01 00:00:00

  • Kit-independent mast cell adhesion mediated by Notch.

    abstract::Kit/CD117 plays a crucial role in the cell-cell and cell-matrix adhesion of mammalian mast cells (MCs); however, it is unclear whether other adhesion molecule(s) perform important roles in the adhesion of MCs. In the present study, we show a novel Kit-independent adhesion mechanism of mouse cultured MCs mediated by No...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Murata A,Hikosaka M,Yoshino M,Zhou L,Hayashi SI

    更新日期:2019-02-15 00:00:00

  • The interface between innate and acquired immunity: glycolipid antigen presentation by CD1d-expressing dendritic cells to NKT cells induces the differentiation of antigen-specific cytotoxic T lymphocytes.

    abstract::In vivo administration of NKT cell ligand, alpha-galactosylceramide (alpha-GalCer), caused the activation of NKT cells to induce a strong NK activity and cytokine production by CD1d-restricted mechanisms. Surprisingly, we also found that alpha-GalCer induced the activation of immunoregulatory cells involved in acquire...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Nishimura T,Kitamura H,Iwakabe K,Yahata T,Ohta A,Sato M,Takeda K,Okumura K,Van Kaer L,Kawano T,Taniguchi M,Nakui M,Sekimoto M,Koda T

    更新日期:2000-07-01 00:00:00

  • The cytoplasmic 'linker region' in Toll-like receptor 3 controls receptor localization and signaling.

    abstract::Toll-like receptor 3 (TLR3) recognizes double-stranded RNA and transmits signals to activate NF-kappaB and the interferon (IFN)-beta promoter via the newly identified adaptor, TICAM-1. The extracellular LRR domain of TLR3 is engaged in the ligand recognition, while the intracellular TIR domain is crucial for the adapt...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Funami K,Matsumoto M,Oshiumi H,Akazawa T,Yamamoto A,Seya T

    更新日期:2004-08-01 00:00:00

  • Functional consequences of the binding of MHC class II-derived peptides to MHC class II.

    abstract::Three MHC class II-derived synthetic peptides (I-A beta (g7)1-16, I-A beta (g7)52-77 and I-A alpha (g7)63-82YC) were analyzed for their ability to bind to syngeneic and allogeneic MHC class II molecules using a whole cell, competitive peptide binding assay. These studies demonstrated that the A beta (g7)1-16 peptide w...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Feili-Hariri M,Kao H,Mietzner TA,Morel PA

    更新日期:1996-12-01 00:00:00

  • Immunity genes and their orthologs: a multi-species database.

    abstract::Metazoan species, from sponges to insects and mammals, possess successful defence systems against their pathogens and parasites. The evolutionary origins of these diverse systems are beginning to be more comprehensively investigated and mapped out. We have collected 1811 metazoan immunity genes from literature and gen...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Rannikko K,Ortutay C,Vihinen M

    更新日期:2007-12-01 00:00:00

  • Requirement of high-affinity IL-2-IL-2R interaction for T cell anergy induction.

    abstract::Incomplete T cell antigen receptor-mediated signaling induces an unresponsive state known as anergy. Previously, we had shown that anergy can be induced in antigen-primed but not naive T cells. In this report, we found that in vitro primed T cells from IL-2R alpha-deficient mice were resistant to anergy induction in c...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Hayashi RJ,Kanagawa O

    更新日期:2006-05-01 00:00:00

  • Specific decrease of Th1-like activity in mice with plasma cell tumors.

    abstract::Previously we examined the ability of the host's immune responses to regulate Ig production in an IgE-secreting murine plasma cell tumor (B53). In the present study we have examined the reverse phenomenon, in that we have investigated the effects of this and other plasma cell tumors on the immune responses of their ho...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Ruzek MC,Mathur A

    更新日期:1995-07-01 00:00:00

  • CD4 T cells inhibit the CD8 T cell response during low-dose virus infection.

    abstract::CD4 T cells are not thought to play a significant role in generating an effective primary CD8 T cell response to most viral infections. We have challenged this view by demonstrating that antigen-specific CD4 T cells can indeed suppress the proliferation of antigen-specific naive CD8 T cells in response to low doses of...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Cose S,Brammer C,Zammit DJ,Blair DA,Lefrançois L

    更新日期:2006-08-01 00:00:00

  • Immune regulatory effects of central nervous system antigens in culture.

    abstract::Evidence from several different experimental systems suggests that regulatory cells specific for self-antigens exist in the normal immune repertoire, and that these cells are necessary for maintenance of self-tolerance and prevention of autoimmune disease. We attempted to demonstrate the existence of regulatory cells ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Lindsey JW,Jin R

    更新日期:2000-11-01 00:00:00

  • A novel co-stimulatory T cell antigen co-expressed on renal cell carcinoma.

    abstract::The A6H mAb raised primarily against human renal cell carcinoma (RCC) has previously been shown to bind strongly to RCC, to some degree to colon carcinoma but only marginally to a variety of normal tissues. Immunohistochemical analysis or RCC tissues containing tumor-infiltrating lymphocytes revealed that A6H stained ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Labuda T,Lando P,Björkdahl O,Kalland T,Vessella R,Hedlund G,Eriksson H,Sjögren HO,Dohlsten M

    更新日期:1995-09-01 00:00:00

  • Adjuvant-dependent modulation of Th1 and Th2 responses to immunization with beta-amyloid.

    abstract::The role of adjuvant on the T(h)1 and T(h)2 immune responses to Abeta-immunotherapy (Abeta(42 )peptide) was examined in wild-type mice. Fine epitope analysis with overlapping oligomers of the Abeta(42) sequence identified the 1-15 region as a dominant B cell epitope. The 6-20 peptide was recognized only weakly by anti...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Cribbs DH,Ghochikyan A,Vasilevko V,Tran M,Petrushina I,Sadzikava N,Babikyan D,Kesslak P,Kieber-Emmons T,Cotman CW,Agadjanyan MG

    更新日期:2003-04-01 00:00:00

  • Tumor escape mechanisms from immunosurveillance: induction of unresponsiveness in a specific MHC-restricted CD4+ human T cell clone by the autologous MHC class II+ melanoma.

    abstract::The co-existence of tumor specific immunity with a progressing tumor is observed in a variety of experimental systems and remains one of the major paradoxes of tumor immunology. We now report that a human melanoma cell line (SMC) expressing MHC class II was able to induce clonal anergy in a specific, MHC-restricted CD...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Becker JC,Brabletz T,Czerny C,Termeer C,Bröcker EB

    更新日期:1993-12-01 00:00:00

  • Allorecognition of human neural stem cells by peripheral blood lymphocytes despite low expression of MHC molecules: role of TGF-beta in modulating proliferation.

    abstract::Neural stem cells (NSCs) transplantation has been proposed as a means of restoring damaged brain tissue, a possibility rendered more likely by reports of low NSCs immunogenicity in various experimental models because of low expression of MHC class I and II as well as co-stimulatory molecules. We investigated the immun...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Ubiali F,Nava S,Nessi V,Frigerio S,Parati E,Bernasconi P,Mantegazza R,Baggi F

    更新日期:2007-09-01 00:00:00

  • Activation-induced expression of murine CD83 on T cells and identification of a specific CD83 ligand on murine B cells.

    abstract::Human CD83 is a cell surface protein expressed predominantly by dendritic cells (DC) and lymphoid cells. So far, there exists no information on the function and distribution of mCD83. Here we demonstrate that mCD83 is moderately expressed on resting T cells and DC, but strongly increases in its expression on T cells f...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Cramer SO,Trumpfheller C,Mehlhoop U,Moré S,Fleischer B,von Bonin A

    更新日期:2000-09-01 00:00:00

  • Physiological up-regulation of inhibitory receptors Fc gamma RII and CR1 on memory B cells is lacking in SLE patients.

    abstract::Under physiological conditions immune complexes (IC) are efficiently cleared from the circulation and meanwhile provide important feedback signals for the immune system via Fc gamma Rs and complement receptors. Dysregulation of these mechanisms have been implicated in conditions where IC concentrations reach pathologi...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Isaák A,Gergely P Jr,Szekeres Z,Prechl J,Poór G,Erdei A,Gergely J

    更新日期:2008-02-01 00:00:00

  • Phenotype of B cells responding to the thymus-independent type-2 antigen polyvinyl pyrrolidinone.

    abstract::We have determined the origin and cell surface phenotype of B cells producing antibody in response to immunization with the non-self TI-2 antigen polyvinyl pyrrolidinone (PVP). We report that the responding cells are derived from precursors in adult bone marrow and display the phenotype characteristic of B-1 cells. By...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Whitmore AC,Haughton G,Arnold LW

    更新日期:1996-04-01 00:00:00

  • Ly-49 antigen defines an alpha beta TCR population in i-IEL with an extrathymic maturation.

    abstract::In this paper we describe a monoclonal antibody, JR9-318, which was obtained from a wild-derived mouse immunized with the M14T cell line (CD3+CD4-CD8-) in order to describe antigenic molecules expressed during T cell differentiation. This mAb precipitated a protein from the M14T cell line or subclones that migrates li...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Roland J,Cazenave PA

    更新日期:1992-06-01 00:00:00