Abstract:
:The mucosa is one of the initial sites of group A streptococcal (GAS) infection and salivary IgA (sIgA) is thought to be critical to immunity. However, the target epitopes of sIgA and the function of sIgA in GAS immunity, in particular the role of accessory cells and complement, is largely unknown. We studied the aquisition and the function of sIgA specific for a conserved region epitope, p145 (sequence: LRRDLDASREAKKQVEKALE) of the M protein. Peptide 145-specific sIgA is highly prevalent within an Aboriginal population living in an area endemic for GAS and acquisition of p145-specific sIgA increases with age, consistent with a role for such antibodies in immunity to GAS. Human sIgA and IgG specific for p145 were affinity purified and shown to opsonize M5 GAS in vitro. Opsonization could be specifically inhibited by the addition of free p145 to the antibodies during assay. Opsonization of GAS was totally dependent on the presence of both complement and polymorphonuclear leukocytes, and, moreover, affinity-purified p145-specific sIgA was shown to fix complement in the presence of M5 GAS. These data show that mucosal IgA to this conserved region peptide within the M protein has an important role in human immunity against GAS and may be useful in a broad-based cross-protective anti-streptococcal vaccine.
journal_name
Int Immunoljournal_title
International immunologyauthors
Brandt ER,Hayman WA,Currie B,Carapetis J,Jackson DC,Do KA,Good MFdoi
10.1093/intimm/11.4.569subject
Has Abstractpub_date
1999-04-01 00:00:00pages
569-76issue
4eissn
0953-8178issn
1460-2377journal_volume
11pub_type
杂志文章abstract::Southern blot analysis of genomic DNA of normal mouse thymocytes with a JH4 probe, a probe for JH4 of Igh genes, consistently showed a second band in addition to a germline band. The same band was also observed in analysis with a 5' DQ probe, a 5'-flanking sequence of DQ52, thus suggesting the use of DQ52. By analyzin...
journal_title:International immunology
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.7.1045
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journal_title:International immunology
pub_type: 杂志文章
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journal_title:International immunology
pub_type: 杂志文章
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journal_title:International immunology
pub_type: 杂志文章
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu086
更新日期:2015-02-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:1994-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh115
更新日期:2004-08-01 00:00:00
abstract::The dynamic behaviour of isolated populations of peritoneal LPS-reactive (LPSr) B lymphocytes was studied upon transfer of peritoneal cells (PerC) from C57BL/6 LPS responder into C57BL/10ScCr LPS non-responder mice. We have followed the persistence and life-span of the transferred LPSr donor B cells in the spleen and ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.1.73
更新日期:1990-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs069
更新日期:2012-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh012
更新日期:2004-01-01 00:00:00
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更新日期:2002-03-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxx045
更新日期:2017-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.9.1255
更新日期:2000-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq033
更新日期:2010-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.9.1425
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更新日期:2013-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.12.2043
更新日期:1999-12-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.4.507
更新日期:1997-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.8.827
更新日期:1991-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxx011
更新日期:2017-02-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.11.1687
更新日期:1997-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.6.957
更新日期:1999-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.11.1303
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