Abstract:
:We established an experimental system in vitro to examine the developmental capacity of individual hematopoietic progenitors to generate T, B and myeloid (M) cells. By using this system we analyzed the process of lineage commitment of hematopoietic progenitors in murine fetal liver (FL). It is known that small numbers of B and M cells, in addition to T cells, are generated in a co-culture of hematopoietic progenitors and a deoxyguanosine-treated fetal thymus (FT) lobe. We tried to enhance the growth of B and M cells by the addition of IL-7, IL-3 and stem cell factor into the co-culture. This cytokine-supplemented FT organ culture was used to examine the developmental capacity of individual hematopoietic progenitors in FL. Single cells of lineage marker (Lin)-c-kit+Sca-1+ (Sca-1+) and Lin-c-kit+Sca-1-(Sca-1-) populations from the FL harvested at day 12 of gestation were cultured for 10 days, and the phenotypes of cells generated in each lobe were analyzed with a flow cytometer. All progenitors in the Sca-1- population were shown to be committed to generate only T, B or M cells. On the other hand, multipotent progenitors, which are capable of generating T, B and M cells, as well as unipotent progenitors committed to the T, B or M lineage were found in the Sca-1+ population. Bipotent progenitors generating M and T cells and those generating M and B cells were also found in the Sca-1+ population, which probably represent progenitors in the process of commitment. However, no bipotent progenitors generating T and B cells were detected.
journal_name
Int Immunoljournal_title
International immunologyauthors
Kawamoto H,Ohmura K,Katsura Ydoi
10.1093/intimm/9.7.1011subject
Has Abstractpub_date
1997-07-01 00:00:00pages
1011-9issue
7eissn
0953-8178issn
1460-2377journal_volume
9pub_type
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journal_title:International immunology
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journal_title:International immunology
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journal_title:International immunology
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journal_title:International immunology
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更新日期:1994-03-01 00:00:00
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pub_type: 杂志文章
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2003-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/4.6.673
更新日期:1992-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.7.1011
更新日期:1994-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.9.1347
更新日期:2000-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章,评审
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更新日期:2020-06-26 00:00:00
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pub_type: 杂志文章
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更新日期:2001-02-01 00:00:00
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更新日期:1999-02-01 00:00:00
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更新日期:1996-12-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2020-10-20 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.12.1253
更新日期:1991-12-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.8.1131
更新日期:1997-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/4.9.975
更新日期:1992-09-01 00:00:00
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journal_title:International immunology
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更新日期:2009-10-01 00:00:00
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journal_title:International immunology
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更新日期:1992-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/5.2.231
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