Abstract:
:IgE plus antigen-stimulated mast cells degranulate, synthesize leukotrienes and secrete cytokines. According to the coalescence model this process is initiated in specific membrane compartments termed rafts. There, enhanced levels of glycosphingolipids and cholesterol stabilize the interaction of FcepsilonRI and Lyn, and thus facilitate the first steps of signal transduction. Enforced changes in raft architecture by cholesterol deprivation and exogenous application of glycosphingolipids influence these early events by loss of tyrosine kinase activity or receptor-independent signal initiation respectively. Here we show that exogenously added cholesterol accumulates in rafts and activates mast cells. An investigation of the signaling events reveals that in contrast to IgE plus antigen-mediated stimulation, cholesterol triggers p38 mitogen-activated protein kinase and preferentially induces expression of FosB. Consequently, a comparative large-scale microarray analysis demonstrates that a number of IgE plus antigen-induced immediate early genes (peak expression at 30 min after induction) are repressed by cholesterol. These changes further translate into altered expression levels and time kinetics of a number of early genes (peak expression at 2 h after stimulation). As the most prominent example for cholesterol-dependent genes, we identified PAI1 (plasminogen activator inhibitor 1), a protein regarded as a risk factor for atherosclerosis.
journal_name
Int Immunoljournal_title
International immunologyauthors
Baumruker T,Csonga R,Pursch E,Pfeffer A,Urtz N,Sutton S,Bofill-Cardona E,Cooke M,Prieschl Edoi
10.1093/intimm/dxg120subject
Has Abstractpub_date
2003-10-01 00:00:00pages
1207-18issue
10eissn
0953-8178issn
1460-2377journal_volume
15pub_type
杂志文章abstract::CD4 and CD8 T cell subsets involved in primary allo-MLR responses were re-evaluated in the present study. The slope analysis of B6 allo-MLR responses to MHC class I-disparate bm1 and class II-disparate bm12 revealed the presence of a T-T cell interaction between CD4 and CD8 T cell subsets. The allo-MLR response of B6 ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.12.1203
更新日期:1990-01-01 00:00:00
abstract::The extracellular signal-regulated kinase (ERK) signaling pathway is strongly activated in response to TCR stimulation in normal T cells. However, the extent to which activation of the ERK pathway is necessary for TCR-stimulated cytokine production is not clear. We have addressed this question by use of two separate m...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.2.223
更新日期:1998-02-01 00:00:00
abstract::Germline CH transcripts initiate from a non-coding I exon and terminate downstream of the associated CH exons. Ig heavy chain class switch recombination from the VDJ-C mu gene to particular downstream CH genes appears to be regulated by a process that involves mitogen and/or cytokine induction of germline CH transcrip...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.4.491
更新日期:1994-04-01 00:00:00
abstract::Deletion of alpha i2 subunit of heterotrimeric G proteins induces a 2-4-fold increase in the proportions of CD4 and CD8 single-positive (SP) thymocytes as compared with wild-type littermates, but how G alpha i2 is involved in thymocyte development is unknown. To determine a role for G alpha i2 in a specific developmen...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh204
更新日期:2005-03-01 00:00:00
abstract::NKT cells are characterized by their expression of an NKT-cell-specific invariant antigen-receptor α chain encoded by Vα14Jα18 gene segments. These NKT cells bridge the innate and acquired immune systems to mediate effective and augmented responses; however, the limited number of NKT cells in vivo hampers their analys...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu057
更新日期:2014-10-01 00:00:00
abstract::The efficacy of cytotoxic T lymphocytes (CTL) has been shown to be highly dependent upon their functional avidity (the sensitivity of their cellular response to MHC-peptide complexes). To examine this relationship, we employed target cell lysis as a quantitative measure and established a set of four CTL clones that ex...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.6.817
更新日期:2001-06-01 00:00:00
abstract::Whether astrocytes utilize B7:CD28 co-stimulation to activate T cells mediating CNS inflammatory disease is controversial. In this report, primary astrocytes and murine astrocyte lines, generated by immortalization at two different times, day 7 or 45 of culture, were examined for their capability to express B7 co-stim...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.7.1169
更新日期:1999-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn078
更新日期:2008-09-01 00:00:00
abstract::After a 3 week course (approximately), during which there is marked lymphoid hyperplasia, Trypanosoma musculi infections in young-adult mice are cured by an immune mechanism involving antibodies of the IgG2a isotype. Both the lymphoid hyperplasia and IgG2a antibody response are T-cell-dependent events and both process...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1107
更新日期:1994-08-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.2.151
更新日期:2000-02-01 00:00:00
abstract::A gelatin sponge model of concomitant tumor immunity was employed in order to examine the clonality of T cells associated with progressing and rejected tumor sites. Here we show that freshly isolated T cells bearing TCR V(beta)1, CDR3 RPGTGN, J(beta)1.1 and TCR V(beta)8, CDR3 GD, J(beta)1.6 predominated progressing an...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.5.639
更新日期:2000-05-01 00:00:00
abstract::T(h)17 cells, an inflammatory T helper cell subset, are involved in the pathogenesis of various inflammatory, autoimmune and allergic diseases. Recent evidence supports the idea that immune cell functions and the inflammatory response are finely regulated by various physiological substances. Calcitonin gene-related pe...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs075
更新日期:2012-11-01 00:00:00
abstract::T lymphocyte responses to the MHC of an evolutionarily distant species are known to be weak compared with responses against allogeneic MHC products within a species. This fact was used to examine the regions of human MHC class II molecules required for the stimulation of strong primary immune responses against MHC all...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.8.935
更新日期:1992-08-01 00:00:00
abstract::NK cells limit the emergence of cancers and viral infections by surveillance of 'missing-self' and 'induced-self' ligands, and by direct recognition of pathogen-associated molecules. We examined individual roles for Toll-like receptors (TLRs)-7 and -8 in human NK-cell activation using synthetic, small molecule agonist...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl046
更新日期:2006-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.10.1529
更新日期:1998-10-01 00:00:00
abstract::Recent studies point to glycolipid-enriched membrane (GEM) microdomains as the critical sites for TCR-mediated signal transduction. However, whether the TCR complex is localized in the GEM domain is not well-defined. In the present study, we analyzed localization of the TCR-CD3 complex in the GEM domain by isolating t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.9.1395
更新日期:1999-09-01 00:00:00
abstract::Toll-like receptor 7 (TLR7) and TLR9 sense microbial single-stranded RNA (ssRNA) and ssDNA in endolysosomes. Nucleic acid (NA)-sensing in endolysosomes is thought to be important for avoiding TLR7/9 responses to self-derived NAs. Aberrant self-derived NA transportation to endolysosomes predisposes to autoimmune diseas...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu086
更新日期:2015-02-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.12.1489
更新日期:2001-12-01 00:00:00
abstract::Auto-antibodies against heat shock proteins (HSPs) are frequently found in the sera of patients with rheumatic and other autoimmune diseases. However, it is unclear whether these auto-antibodies play a role in the pathophysiology and etiology of these diseases. We found that a murine anti-HSP60 mAb enhanced the produc...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh399
更新日期:2006-04-01 00:00:00
abstract::Previously we observed elevation of the serum concentration of two acute-phase protein (AFP) complement components (C9 and C1-inhibitor) in patients with chronic hepatitis C who responded (R) to IFN-alpha therapy, but not in non-responders (NR). In the present study we investigated the effect of high-dose IFN-alpha th...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh024
更新日期:2004-01-01 00:00:00
abstract::The origin of anti-DNA antibodies remains speculative. We argue that some of these antibodies may arise inadvertently in nature during the course of a normal immune response due to their induction by antibodies which bear structures (mimotopes) that mimic DNA. These antibodies are not necessarily DNA specific but, lik...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.9.1099
更新日期:2001-09-01 00:00:00
abstract::CD70 and CD80 are co-stimulatory molecules which belong to the tumor necrosis factor family and the B7 family respectively. When they are co-expressed by gene-modified TS/A tumor cells, they provide an efficient protective and long-lasting T-dependent antitumor response. We first showed that when CD70 and CD80 were de...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg038
更新日期:2003-03-01 00:00:00
abstract::Activated macrophages exert strong arginase (ASE) activity that converts L-arginine into ornithine, the key precursor for putrescine and polyamine biosynthesis. Macrophages were previously also shown to generate nitric oxide that is derived from the guanido group of arginine by the oxidative deiminase (OAD) reaction. ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.5.413
更新日期:1991-05-01 00:00:00
abstract::The distribution of IgE FcR (Fc epsilon R)-positive and -negative B cells was examined in normal adult mice. Using three-color flow cytometry, the expression of the Fc epsilon R was analyzed on various B-cell subsets present in the peritoneum and spleen. The results demonstrate that in the peritoneal cavity, the Fc ep...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.4.305
更新日期:1991-04-01 00:00:00
abstract:BACKGROUND:Intranasal administration of high amount of allergen was shown to induce tolerance and to reverse the allergic phenotype. However, mechanisms of tolerance induction via the mucosal route are still unclear. OBJECTIVES:To characterize the therapeutic effects of intranasal application of ovalbumin (OVA) in a m...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq453
更新日期:2011-01-01 00:00:00
abstract::p40 was previously described as a regulatory molecule capable of inhibiting both the natural and the CD16-mediated cytotoxicity of NK cells. In this study, we analyze the effect of p40 molecule engagement on the NK cell triggering induced by activating HLA class I-specific NK receptors (NKR) or on TCR alpha beta-media...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.9.1271
更新日期:1997-09-01 00:00:00
abstract::It is increasingly evident that neutrophils are able to cross-talk with other leukocytes to shape ongoing inflammatory and immune responses. In this study, we analyzed whether human NK cells may influence the survival and activation of neutrophils under co-culture conditions. We report that NK cells exposed to either ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq434
更新日期:2010-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.6.807
更新日期:1996-06-01 00:00:00
abstract::BALB/c mice injected at birth with 10(8) semi-allogeneic (C57BL/6 x BALB.IgHb)F1 spleen cells develop a lupus-like syndrome in which autoantibodies bear exclusively the donor allotype. We have analyzed the evolution of donor B cell chimerism and the autoimmune manifestations during the first year of life in these mice...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.1.67
更新日期:1992-01-01 00:00:00