Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation.


:Recent studies point to glycolipid-enriched membrane (GEM) microdomains as the critical sites for TCR-mediated signal transduction. However, whether the TCR complex is localized in the GEM domain is not well-defined. In the present study, we analyzed localization of the TCR-CD3 complex in the GEM domain by isolating the GEM fraction with sucrose density gradient centrifugation. Although 10% of TCRzeta chains was localized in the GEM fraction, most of the TCR complexes were excluded from the GEM before and after T cell activation, and the amount of TCRzeta in the GEM was not increased after activation. However, the tyrosine-phosphorylated form of TCRzeta was strongly concentrated in the GEM fraction upon TCR engagement. A kinetic study revealed that tyrosine phosphorylation of TCRzeta occurred initially in the Triton X-100-soluble membrane fraction followed by the accumulation of phosphorylated TCRzeta in the GEM. Thus, these results indicate that phosphorylated TCRzeta migrates into the GEM domains on T cell activation. We speculate that the GEM microdomains may function as a reservoir of activation signals from triggered TCR.


Int Immunol


International immunology


Kosugi A,Saitoh S,Noda S,Yasuda K,Hayashi F,Ogata M,Hamaoka T




Has Abstract


1999-09-01 00:00:00












  • Interleukin 6 in inflammation, autoimmunity and cancer.

    abstract::Interleukin 6 (IL-6) is involved both in immune responses and in inflammation, hematopoiesis, bone metabolism and embryonic development. IL-6 plays roles in chronic inflammation (closely related to chronic inflammatory diseases, autoimmune diseases and cancer) and even in the cytokine storm of corona virus disease 201...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Hirano T

    更新日期:2020-12-18 00:00:00

  • IL-2 production by myofibroblasts from post-radiation fibrosis in breast cancer patients.

    abstract::The origin of cell activation in post-radiation fibrosis and its chronic extension are still poorly understood. Since local IL-2 cancer treatment sometimes triggers intraperitoneal fibrosis we have analyzed three myofibroblastic cell strains from post-radiation skin fibrosis (FPR7, FPR10 and FPR15) for their interacti...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Alileche A,Han D,Plaisance S,Assier E,Sahraoui Y,Clemanceau C,Metivier D,Brouty-Boyer D,Jasmin C,Azzarone B

    更新日期:1994-10-01 00:00:00

  • Molecular cloning of cDNA encoding MS2 antigen, a novel cell surface antigen strongly expressed in murine monocytic lineage.

    abstract::cDNA clones which strongly hybridized with a 3.1 kb mRNA from mouse macrophages and macrophage cell lines and weakly with mRNA from P815 but not from a variety of other cell lines and tissues were isolated from cDNA libraries constructed using mRNA from murine macrophage cell lines and peritoneal macrophages. Treatmen...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Yoshida S,Setoguchi M,Higuchi Y,Akizuki S,Yamamoto S

    更新日期:1990-01-01 00:00:00

  • PD-1 and PD-1 ligands: from discovery to clinical application.

    abstract::Programmed cell death-1 (PD-1, Pdcd1), an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. Because of the wide range of ligand distribution in the body, its b...

    journal_title:International immunology

    pub_type: 杂志文章,评审


    authors: Okazaki T,Honjo T

    更新日期:2007-07-01 00:00:00

  • Human milk proteins including secretory IgA fail to elicit tolerance after feeding.

    abstract::Oral administration of large doses of protein antigen generally induces a state of systemic unresponsiveness currently termed mucosally induced tolerance. In this study, we used human milk protein (HMP) without casein as a multi-protein antigen for the study of mucosally induced tolerance. The HMP utilized in this stu...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Yuki Y,Fujihashi K,Yamamoto M,McGhee JR,Kiyono H

    更新日期:1998-04-01 00:00:00

  • Glycosyltransferase regulation mediated by pre-TCR signaling in early thymocyte development.

    abstract::CT1 is a carbohydrate moiety of CD45 that is expressed on fetal thymocytes in vivo. Examination of CT1 expression on thymocyte subsets revealed that primarily pro-T cells (CD44+ CD25+) and pre-T cells (CD44- CD25+) expressed CT1. Interestingly, non-T-lineage committed lymphoid progenitors (CD44+ CD25-) lacked CT1 indi...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Reed DS,Olson S,Lefrançois L

    更新日期:1998-04-01 00:00:00

  • Arid5a-deficient mice are highly resistant to bleomycin-induced lung injury.

    abstract::Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are among the major causes of death worldwide due to acute inflammation in the lung. AT-rich interactive domain-containing 5a (Arid5a) is an RNA-binding protein involved in inflammatory autoimmune disease through post-transcriptional control of Il6...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Dubey PK,Masuda K,Nyati KK,-Uz Zaman MM,Chalise JP,Millrine D,Kai W,Ripley B,Kishimoto T

    更新日期:2017-02-01 00:00:00

  • S1P lyase in thymic perivascular spaces promotes egress of mature thymocytes via up-regulation of S1P receptor 1.

    abstract::Sphingosine 1-phosphate (S1P) and S1P receptor 1 (S1P1) play an important role in the egress of mature CD4 or CD8 single-positive (SP) thymocytes from the thymus. Fingolimod hydrochloride (FTY720), an S1P1 functional antagonist, induced significant accumulation of CD62L(high)CD69(low) mature SP thymocytes in the thymi...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Maeda Y,Yagi H,Takemoto K,Utsumi H,Fukunari A,Sugahara K,Masuko T,Chiba K

    更新日期:2014-05-01 00:00:00

  • Preferred CDRH3 lengths for antibodies with defined specificities.

    abstract::The third complementarity determining regions of heavy chain variable regions (CDRH3) confer the fine specificities of antibodies. The CDRH3 length distribution of all human or all mouse antibodies appears to be more or less random, with the human distribution having a wider spread. Since the Kabat database of aligned...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Johnson G,Wu TT

    更新日期:1998-12-01 00:00:00

  • MHC class II and invariant chain biosynthesis and transport during maturation of human precursor dendritic cells.

    abstract::Dendritic cells (DC) are highly potent activators of the immune response. The precise mechanisms that give rise to the DC phenotype are not known. To investigate the mechanisms that contribute to the generation of the DC phenotype, precursor DC were freshly isolated from human blood and allowed to mature in vitro. The...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Engering AJ,Richters CD,Fluitsma DM,van Pelt AM,Kamperdijk EW,Hoefsmit EC,Pieters J

    更新日期:1998-11-01 00:00:00

  • Transendothelial migration of effector T cells across inflamed endothelial barriers does not require heparan sulfate proteoglycans.

    abstract::Leukocyte diapedesis is a chemotactic multistep process that requires optimal chemoattractant presentation by the endothelial barrier. Recent studies have described a critical role for heparan sulfate glycosaminoglycans (HSGAGs) in the presentation and functions of chemokines essential for lymphocyte interactions with...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Stoler-Barak L,Barzilai S,Zauberman A,Alon R

    更新日期:2014-06-01 00:00:00

  • The involvement of the proto-oncogene p120 c-Cbl and ZAP-70 in CD2-mediated T cell activation.

    abstract::The CD2 co-receptor expressed on the surface of T lymphocytes is able to stimulate T cell activation, proliferation and cytokine production in the absence of direct engagement of the antigen-specific TCR. Engagement of human CD2 by mitogenic pairs of anti-CD2 mAb induces tyrosine phosphorylation of a number of intrace...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Lin H,Martelli MP,Bierer BE

    更新日期:2001-01-01 00:00:00

  • Protein kinase D2 contributes to either IL-2 promoter regulation or induction of cell death upon TCR stimulation depending on its activity in Jurkat cells.

    abstract::Members of protein kinase D (PKD) family serine/threonine kinases (PKD1, PKD2 and PKD3) are expressed in wide range of cells and regulate various cellular responses including immune responses. We have previously shown that PKD is involved in the signaling pathways of a human CD4(+) T cell clone stimulated with its cog...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Irie A,Harada K,Tsukamoto H,Kim JR,Araki N,Nishimura Y

    更新日期:2006-12-01 00:00:00

  • Expression of KIR2DS1 does not significantly contribute to NK cell cytotoxicity in HLA-C1/C2 heterozygous haplotype B donors.

    abstract::NK cells are functionally controlled by the killer immunoglobulin-like receptor (KIR) family that comprises inhibitory (iKIR) and activating (aKIR) members. Genetic association studies suggest that donors expressing aKIRs next to iKIRs will be superior donors in the setting of hematopoietic stem cell transplantation o...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Baltner K,Kübler A,Pal M,Balvociute M,Mezger M,Handgretinger R,André MC

    更新日期:2017-11-01 00:00:00

  • CD28 co-stimulatory signals induce IL-2 receptor expression on antigen-stimulated virgin T cells by an IL-2-independent mechanism.

    abstract::Intravenous sensitization of C57BL/6 (B6) mice with class II H-2-disparate B6-C-H-2bm12(bm12) resting B cells induced anti-bm12 CD4+ T cell tolerance as shown by hyporesponsiveness in the anti-bm12 mixed lymphocyte reaction (MLR). The present study investigated the mechanism(s) of the failure of bm12 B cells to stimul...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Toyooka K,Maruo S,Iwahori T,Yamamoto N,Tai XG,Abe R,Takahama Y,Murakami M,Uede T,Hamaoka T,Fujiwara H

    更新日期:1996-02-01 00:00:00

  • CD4 T cells inhibit the CD8 T cell response during low-dose virus infection.

    abstract::CD4 T cells are not thought to play a significant role in generating an effective primary CD8 T cell response to most viral infections. We have challenged this view by demonstrating that antigen-specific CD4 T cells can indeed suppress the proliferation of antigen-specific naive CD8 T cells in response to low doses of...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Cose S,Brammer C,Zammit DJ,Blair DA,Lefrançois L

    更新日期:2006-08-01 00:00:00

  • IL-18-independent cytotoxic T lymphocyte activation and IFN-gamma production during experimental acute graft-versus-host disease.

    abstract::Acute graft-versus-host disease (GvHD) is a serious complication after allogeneic bone marrow transplantation. Donor-derived T cells infiltrate recipient target organs and cause severe tissue damage, often leading to death of the affected patient. Tissue destruction is a direct result of donor CD8+ T cell activation a...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Arnold D,Wasem C,Juillard P,Graber P,Cima I,Frutschi C,Herren S,Jakob S,Alouani S,Mueller C,Chvatchko Y,Brunner T

    更新日期:2002-05-01 00:00:00

  • Self-restricted primary human histocompatibility leukocyte antigen (HLA)-specific cytotoxic T lymphocytes.

    abstract::The specificity of self MHC-restricted T cells responding to peptide fragments of processed antigen has been well characterized. However, the means by which alloreactive T lymphocytes recognize MHC alloantigen remain poorly understood. We now provide evidence that the recognition of class I human leukocyte antigen (HL...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Breur-Vriesendorp BS,Ivanyi P

    更新日期:1993-01-01 00:00:00

  • Longitudinal analysis of T cells responding to tetanus toxoid in healthy subjects as well as in pediatric patients after bone marrow transplantation: the identification of identical TCR-CDR3 regions in time suggests long-term stability of at least part of

    abstract::To understand the nature of long-term Th immune responses, we investigated in the present study the TCRBV gene repertoire of CD4(+) T cells specific for the recall antigen tetanus toxoid (TT) in recipients of an allogeneic bone marrow transplantation (allo-BMT) at several time points after transplantation and in their...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Godthelp BC,van Tol MJ,Vossen JM,van den Elsen PJ

    更新日期:2001-04-01 00:00:00

  • Requirement of high-affinity IL-2-IL-2R interaction for T cell anergy induction.

    abstract::Incomplete T cell antigen receptor-mediated signaling induces an unresponsive state known as anergy. Previously, we had shown that anergy can be induced in antigen-primed but not naive T cells. In this report, we found that in vitro primed T cells from IL-2R alpha-deficient mice were resistant to anergy induction in c...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Hayashi RJ,Kanagawa O

    更新日期:2006-05-01 00:00:00

  • Role of the CD5 molecule on TCR gammadelta T cell-mediated immune functions: development of germinal centers and chronic intestinal inflammation.

    abstract::Although CD4(+) T cells form a major subset of TCRalphabeta T cells, only a small number of TCRgammadelta T cells express CD4. Factors contributing to the down-regulation of CD4(+) TCRgammadelta T cells have not been identified. The CD5 molecule is expressed on most TCRgammadelta T cells in the spleen, whereas only a ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Mizoguchi A,Mizoguchi E,de Jong YP,Takedatsu H,Preffer FI,Terhorst C,Bhan AK

    更新日期:2003-01-01 00:00:00

  • Association of a tetraspanin CD9 with CD5 on the T cell surface: role of particular transmembrane domains in the association.

    abstract::CD9 is a member of the tetraspanin superfamily which is characterized by four transmembrane (TM) domains and associates with other surface molecules. This tetraspanin was recently found to be expressed on mature T cells. Here, we investigated which molecules associate with CD9 on T cells and which CD9 domains are requ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Toyo-oka K,Yashiro-Ohtani Y,Park CS,Tai XG,Miyake K,Hamaoka T,Fujiwara H

    更新日期:1999-12-01 00:00:00

  • Molecular and physiological properties of murine CD14.

    abstract::We have previously cloned the murine homolog of cDNA for the human myelomonocytic differentiation antigen, CD14. We synthesized three hydrophilic peptides derived from the predicted amino acid sequence of murine CD14 (mCD14), designated MS7.1, MS7.2, and MS7.3, respectively, and raised antisera against them. Each anti...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Nasu N,Yoshida S,Akizuki S,Higuchi Y,Setoguchi M,Yamamoto S

    更新日期:1991-02-01 00:00:00

  • Multiple nuclear factors bind to novel positive and negative regulatory elements upstream of the human MHC class I gene HLA-A11.

    abstract::Quantitative expression of class I genes varies widely in different tissues, during development and in some neoplastic cells. Regulatory DNA sequences controlling levels of transcription of class I genes have been characterized in the murine and swine promoters. Although some regulatory features appear to be retained ...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Blanchet O,Gazin C,L'Haridon M,Tatari Z,Degos L,Sigaux F,Paul P

    更新日期:1994-10-01 00:00:00

  • Expression of a type II collagen-specific TCR transgene accelerates the onset of arthritis in mice.

    abstract::Animal models of autoimmune diseases have been instrumental in advancing our understanding of autoimmunity in humans. Collagen-induced arthritis in mice is an autoimmune disease model of rheumatoid arthritis, which is MHC class II restricted and CD4 T cell dependent. To better understand the fundamental role of T cell...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Osman GE,Cheunsuk S,Allen SE,Chi E,Liggitt HD,Hood LE,Ladiges WC

    更新日期:1998-11-01 00:00:00

  • Skewed V beta TCR repertoire of CD8+ T cells in murine Trypanosoma cruzi infection.

    abstract::We have followed CD4 and CD8 TCR V beta repertoires during the acute phase of Trypanosoma cruzi infection in a resistant mouse strain (C57BL/6). No major changes were found in the V beta TCR distributions analyzed (covering roughly 40% of the TCR repertoire) in peripheral CD4 T lymphocytes, confirming the polyclonal n...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Leite-de-Moraes MC,Coutinho A,Hontebeyrie-Joskowicz M,Minoprio P,Eisen H,Bandeira A

    更新日期:1994-03-01 00:00:00

  • Development of Th1 and not Th2 immune responses in mice lacking IFN-regulatory factor-4.

    abstract::IFN-regulatory factor (IRF)-4 is a member of the IRF family of transcription factors expressed in lymphocytes and macrophages. The previous studies using mice deficient in the IRF-4 gene showed profound defects in function of both B and T cells. To further investigate the role of IRF-4 in CD4(+) T cell function, IRF-4...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Tominaga N,Ohkusu-Tsukada K,Udono H,Abe R,Matsuyama T,Yui K

    更新日期:2003-01-01 00:00:00

  • HLA-DM and MHC class II molecules co-distribute with peptidase-containing lysosomal subcompartments.

    abstract::MHC class II molecules associate with peptides in the endocytic pathway. Different endosomal locations for peptide loading of class II molecules, varying from early endosomes (EE) to lysosomes, have been assigned on the basis of subcellular fractionation experiments. We have determined the intracellular location of HL...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Fernandez-Borja M,Verwoerd D,Sanderson F,Aerts H,Trowsdale J,Tulp A,Neefjes J

    更新日期:1996-05-01 00:00:00

  • Peptides based on the complementarity-determining regions of a pathogenic autoantibody mitigate lupus manifestations of (NZB x NZW)F1 mice via active suppression.

    abstract::Two peptides based on the complementarity-determining regions (CDR) 1 and 3 (pCDR1 and pCDR3) of a murine monoclonal anti-DNA autoantibody that expresses the common idiotype 16/6Id were shown to down-regulate systemic lupus erythematosus (SLE)-associated T cell responses and to prevent the development of clinical symp...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Zinger H,Eilat E,Meshorer A,Mozes E

    更新日期:2003-02-01 00:00:00

  • A novel approach to induce human DCs from monocytes by triggering 4-1BBL reverse signaling.

    abstract::Dendritic cells (DCs) are responsible for the initiation of immune responses. Our study demonstrates a new pathway for generating a large quantity of stimulatory monocyte-derived DCs (Mo-DCs) from human monocytes using anti-4-1BB ligand (4-1BBL) mAb to trigger reverse signaling. The anti-4-1BBL-driven Mo-DCs (DCs(alph...

    journal_title:International immunology

    pub_type: 杂志文章


    authors: Ju S,Ju S,Ge Y,Qiu H,Lu B,Qiu Y,Fu J,Liu G,Wang Q,Hu Y,Shu Y,Zhang X

    更新日期:2009-10-01 00:00:00