Abstract:
:IL-12 has been shown to play a central role in cell-mediated inflammatory reactions through direct activation of T cells and NK cells. IL-12 also strongly influences humoral immunity but these effects have been thought to be indirect and caused by intermediary cytokines. Using flow cytometry, we now show that IL-12 directly interacts with B cells. Freshly isolated murine peritoneal B-1 and conventional B lymphocytes bound IL-12, but splenic B cells failed to react unless first stimulated with lipopolysaccharide. All murine B cell sources were found to express IL-12R beta 1 subunit transcripts as detected by PCR and RNase protection assays. IL-12 binding was also detected on phytohemagglutinin-stimulated human T cell blasts and Staphylococcus aureusl IL-2-stimulated B cell blasts but not on freshly isolated peripheral blood lymphocytes. Similarly, IL-12 directly bound to the human SKW6.4 Burkitt's B cell lymphoma line. In all cases positive staining was ablated by omitting IL-12 from the procedure, showing that it was not due to detection of endogenous IL-12. These findings indicate that B cells represent another major target for IL-12 in addition to T and NK cells, and that IL-12 can directly affect humoral immunity.
journal_name
Int Immunoljournal_title
International immunologyauthors
Vogel LA,Showe LC,Lester TL,McNutt RM,Van Cleave VH,Metzger DWdoi
10.1093/intimm/8.12.1955subject
Has Abstractpub_date
1996-12-01 00:00:00pages
1955-62issue
12eissn
0953-8178issn
1460-2377journal_volume
8pub_type
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pub_type: 杂志文章,评审
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更新日期:2000-03-01 00:00:00
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doi:10.1093/intimm/dxh213
更新日期:2005-04-01 00:00:00
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更新日期:1995-02-01 00:00:00
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更新日期:1990-01-01 00:00:00
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