Abstract:
:Taking advantage of the recently demonstrated presence of N-aminopeptidases and the serine protease dipeptidyl aminopeptidase IV (DPP IV) at the surface of human myeloblastic HL-60 cells, the regulation of these protease activities in HL-60 cell differentiation has been assessed using combined spectrophotometric and flow cytometric assays. Addition of human recombinant granulocyte macrophage colony stimulating factor (rHu-GM-CSF) to HL-60 cells to induce differentiation into macrophages led to a time- and dose-dependent increase in both cell surface N-aminopeptidase and DPP IV activities. Protease up-regulation was due to an enhancement in cell surface protease number, associated with a slight rise in apparent affinities of the enzymes for their substrates. In contrast, in HL-60 cells induced to differentiate into neutrophils in the presence of retinoic acid, expression of cell surface N-aminopeptidases was almost completely abolished in a time- and dose-dependent fashion, and this down-regulation was accompanied by a weak but significant decrease in affinity. However, no noticeable difference was seen in serine DPP IV expression between retinoic acid-treated and untreated HL-60 cells. Retinoic acid treatment also reduced soluble protease activity in vitro indicating that down-regulation of membrane aminopeptidases was not due to their proteolytic clip. No modulation in the activity of any of the enzymes tested was seen with human recombinant tumor necrosis factor-alpha or retinol which do not induce HL-60 cell differentiation.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Int Immunoljournal_title
International immunologyauthors
Laouar A,Wietzerbin J,Bauvois Bdoi
10.1093/intimm/5.8.965subject
Has Abstractpub_date
1993-08-01 00:00:00pages
965-73issue
8eissn
0953-8178issn
1460-2377journal_volume
5pub_type
杂志文章abstract::Three MHC class II-derived synthetic peptides (I-A beta (g7)1-16, I-A beta (g7)52-77 and I-A alpha (g7)63-82YC) were analyzed for their ability to bind to syngeneic and allogeneic MHC class II molecules using a whole cell, competitive peptide binding assay. These studies demonstrated that the A beta (g7)1-16 peptide w...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.12.1857
更新日期:1996-12-01 00:00:00
abstract::Rheumatic fever (RF) follows a throat infection with different M-serotypes of beta-hemolytic group A streptococci (GAS) and can affect different tissues, predominantly the heart. It is thought to be an autoimmune illness. Although histological examination of affected heart shows an infiltrate consisting mainly of T ce...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.8.1235
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abstract::Dendritic cells (DC) are key players in the initiation and modulation of adaptive immune responses due to their ability to acquire and present antigen and stimulate T cells. For the induction of effector T cell functions, antigen must be presented by activated DC. In this study, we have compared uptake of antigen by m...
journal_title:International immunology
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doi:10.1093/intimm/dxp053
更新日期:2009-07-01 00:00:00
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journal_title:International immunology
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journal_title:International immunology
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doi:10.1093/intimm/7.8.1331
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更新日期:1999-10-01 00:00:00
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journal_title:International immunology
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doi:10.1093/intimm/dxh326
更新日期:2005-11-01 00:00:00
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更新日期:1998-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2020-10-20 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.2.281
更新日期:1997-02-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.10.1259
更新日期:1993-10-01 00:00:00
abstract::Investigation of the process of Peyer's patch (PP) formation has been hampered by difficulties in identifying its initial step in the embryo. In this study, we overcame this problem by means of whole-mount immunohistochemistry using mAb against the molecules which were expressed in the cells accumulated at the site of...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.4.507
更新日期:1997-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxv051
更新日期:2016-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.12.1705
更新日期:2000-12-01 00:00:00
abstract::Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A bot...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxv014
更新日期:2015-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.4.331
更新日期:1993-04-01 00:00:00
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journal_title:International immunology
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doi:10.1093/intimm/12.7.1085
更新日期:2000-07-01 00:00:00
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更新日期:1998-03-01 00:00:00
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更新日期:1993-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.11.1751
更新日期:1994-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2004-02-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:1998-02-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:International immunology
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.10.1485
更新日期:1994-10-01 00:00:00
abstract::A gelatin sponge model of concomitant tumor immunity was employed in order to examine the clonality of T cells associated with progressing and rejected tumor sites. Here we show that freshly isolated T cells bearing TCR V(beta)1, CDR3 RPGTGN, J(beta)1.1 and TCR V(beta)8, CDR3 GD, J(beta)1.6 predominated progressing an...
journal_title:International immunology
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doi:10.1093/intimm/12.5.639
更新日期:2000-05-01 00:00:00