Abstract:
:Notch2 and Delta-like 1 (Dll1) have been implicated in the development of marginal zone B (MZB) cells. In the present study, we characterized the expression and function of mouse Notch receptors and ligands in the spleen by using newly generated mAbs. Although Notch2 was expressed on both B and T cells in the spleen, the highest expression was observed on precursors of marginal zone B and MZB cells. Dll1 was expressed on macrophage and erythroblasts in the red pulp, but not on B cells or marginal zone macrophage. Administration of a blocking mAb against Dll1 not only blocked the development of MZB cells in juvenile mice but also gradually depleted the pre-established MZB cells in adult mice, indicating a critical role for Dll1 in the maintenance of MZB cells in the spleen of normal mice. Interestingly, Dll1 was not necessary for the maintenance of MZB cells in lupus-prone (NZB x NZW) F1 mice particularly after the onset of the disease, suggesting that the Dll1 independence may be a feature of dysregulated MZB cells producing auto-antibodies.
journal_name
Int Immunoljournal_title
International immunologyauthors
Moriyama Y,Sekine C,Koyanagi A,Koyama N,Ogata H,Chiba S,Hirose S,Okumura K,Yagita Hdoi
10.1093/intimm/dxn034subject
Has Abstractpub_date
2008-06-01 00:00:00pages
763-73issue
6eissn
0953-8178issn
1460-2377pii
dxn034journal_volume
20pub_type
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