Abstract:
:In this study, we show that IFNalpha increases the chemotaxis of human B cells to CCL20, CCL21 and CXCL12 in a dose- and time-dependent manner. The effect was maximal with 2000 IU ml(-1) IFNalpha. It peaked at 24 h and decreased thereafter. At 24 h, IFNalpha had increased B-cell chemotaxis to CCL20 by 20 +/- 6.2% (n = 9, P < 0.002), to CCL21 by 20 +/- 8.5% (n = 14, P < 0.0001) and to CXCL12 by 16.3 +/- 4.2% (n = 12, P < 0.003) without changing CCR6, CCR7 or CXCR4 expression. IFNalpha enhanced the migration of memory B cells to CCL20, CCL21 and CXCL12 2.6-fold more strongly than that of naive B cells. The triggering of chemokine receptors by their ligands resulted in the activation of phosphatidylinositide-3 kinase (PI3K)/protein kinase B (PKB), inhibitory NF-kappaB (IkappaBalpha) RhoA and extracellular signal-regulated protein kinase 1/2 (ERK1/2). All these effectors except ERK1/2 are crucial for B-cell chemotaxis. IFNalpha modulated the requirements for B-cell chemotaxis, which became dependent on ERK1/2, more dependent on PI3K, RhoA and nuclear factor-kappaB but less dependent on Gbetagamma and phospholipase C activation. IFNalpha also decreased ligand-induced chemokine receptor internalization in a manner dependent on PI3K/AKT and RhoA but not on IkappaBalpha and ERK1/2. Our data characterize chemokine receptor signaling in human B cells and clarify the relevance of downstream pathways in B-cell chemotaxis and chemokine receptor internalization. They also suggest that non-class I PI3K are involved in B-cell chemotaxis.
journal_name
Int Immunoljournal_title
International immunologyauthors
Badr G,Borhis G,Treton D,Richard Ydoi
10.1093/intimm/dxh227subject
Has Abstractpub_date
2005-04-01 00:00:00pages
459-67issue
4eissn
0953-8178issn
1460-2377pii
dxh227journal_volume
17pub_type
杂志文章abstract::The co-existence of tumor specific immunity with a progressing tumor is observed in a variety of experimental systems and remains one of the major paradoxes of tumor immunology. We now report that a human melanoma cell line (SMC) expressing MHC class II was able to induce clonal anergy in a specific, MHC-restricted CD...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.12.1501
更新日期:1993-12-01 00:00:00
abstract::IgE plus antigen-stimulated mast cells degranulate, synthesize leukotrienes and secrete cytokines. According to the coalescence model this process is initiated in specific membrane compartments termed rafts. There, enhanced levels of glycosphingolipids and cholesterol stabilize the interaction of FcepsilonRI and Lyn, ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg120
更新日期:2003-10-01 00:00:00
abstract::The role of adjuvant on the T(h)1 and T(h)2 immune responses to Abeta-immunotherapy (Abeta(42 )peptide) was examined in wild-type mice. Fine epitope analysis with overlapping oligomers of the Abeta(42) sequence identified the 1-15 region as a dominant B cell epitope. The 6-20 peptide was recognized only weakly by anti...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg049
更新日期:2003-04-01 00:00:00
abstract::The ability of cytokines to steer CD4(+) T(h) cell responses toward a T(h)1 or T(h)2 phenotype and enhance the magnitude of both CD8(+) cytotoxic T lymphocytes (CTL) and antibody responses has clearly been demonstrated by our lab and others, but the influence of cytokines on protective immune responses is much less cl...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.7.897
更新日期:2001-07-01 00:00:00
abstract::Absence of transforming growth factor-beta (TGF-beta) signaling to T cells in mice results in an increase in T cell numbers, an activated CD44 high, CD69-, CD25- T cell phenotype and a T cell-mediated injury to many organs. It is not known if such T cell activation in the absence of TGF-beta signaling is spontaneous o...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh233
更新日期:2005-05-01 00:00:00
abstract::The chicken represents the best-characterized animal model for B cell development in the so-called gut-associated lymphoid tissue (GALT) and the molecular processes leading to B cell receptor diversification in this species are well investigated. However, the mechanisms regulating B cell development and homeostasis in...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl137
更新日期:2007-02-01 00:00:00
abstract::Naive and primed alpha beta T cells can be distinguished on the basis of their differential expression of CD45RA and CD45RO, respectively. The present study indicates that these CD45-isoforms also identify naive and primed maturational stages of gamma delta T cells and natural killer (NK) cells. In peripheral blood, a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.7.691
更新日期:1991-07-01 00:00:00
abstract::Mice with a targeted deletion of the T-bet gene exhibit spontaneous airway hyperresponsiveness (AHR), airway inflammation, enhanced recovery of T(h)2 cytokines from bronchoalveolar lavage fluid, sub-epithelial collagen deposition and myofibroblast transformation. Here we analyze the mechanisms responsible for the chro...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh281
更新日期:2005-08-01 00:00:00
abstract::Liver fibrosis is commonly observed in chronic liver disease. However, the immunological mechanisms underlying hepatic fibrosis due to chronic inflammation are not well defined, mainly because suitable experimental models have not been established. We have found that weekly i.v. administration of concanavalin A (Con A...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.9.1491
更新日期:1999-09-01 00:00:00
abstract::Apoptosis is a major mechanism whereby HIV-1 depletes uninfected CD4+ and CD8+ T cells. We previously showed that resting peripheral blood T cells derived from healthy donors were killed by an apoptotic mechanism after adsorption to gp120-containing, protease-defective HIV-1 (L-2) particles, more effectively than pare...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.10.1453
更新日期:1997-10-01 00:00:00
abstract::Semaphorin 3A (Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A bot...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxv014
更新日期:2015-09-01 00:00:00
abstract::CD9 is a member of the tetraspanin superfamily which is characterized by four transmembrane (TM) domains and associates with other surface molecules. This tetraspanin was recently found to be expressed on mature T cells. Here, we investigated which molecules associate with CD9 on T cells and which CD9 domains are requ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.12.2043
更新日期:1999-12-01 00:00:00
abstract::The specificity of self MHC-restricted T cells responding to peptide fragments of processed antigen has been well characterized. However, the means by which alloreactive T lymphocytes recognize MHC alloantigen remain poorly understood. We now provide evidence that the recognition of class I human leukocyte antigen (HL...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.1.103
更新日期:1993-01-01 00:00:00
abstract::Ig are multifunctional molecules with distinct properties assigned to individual domains. To assess the importance of IgM domain assembly in B cell development we generated two transgenic mouse lines with truncated muH chains by homologous integration of the neomycin resistance gene (neo(r)) into exons C(mu)1 and C(mu...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.12.1489
更新日期:2001-12-01 00:00:00
abstract::Lymphocyte migration from the blood into specific tissues is directed by their expression of adhesion molecules referred to as homing receptors. The homing receptor L-selectin, for example, directs the migration of lymphocytes into peripheral lymph nodes (PLN). Since bovine gamma delta T cells, a major lymphocyte subs...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.1.81
更新日期:1994-01-01 00:00:00
abstract::We asked herein whether functional dendritic-like cells could be generated from t(9;22) acute lymphoblastic leukemia (ALL) blasts. We first determined that the combination of interleukin (IL)-1beta, IL-3, IL-7, tumor necrosis factor-alpha, stem cell factor and CD40 ligand was optimal for generating dendritic-like cell...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh139
更新日期:2004-10-01 00:00:00
abstract::Incompatibility of human minor histocompatibility (hmH) antigens induces rejection of grafts in organ transplantation and graft versus host disease in bone marrow transplantation if donor and recipient are matched for human leukocyte antigen (HLA) genes. These antigens are recognized only by T cells. We describe here ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.2.301
更新日期:1992-02-01 00:00:00
abstract::Antibody responses to ingested antigens can be inhibited by a mechanism known as oral tolerance which acts to prevent excessive stimulation from luminal contents. Local IgA responses can be induced in this non-responsive environment and during intestinal inflammation, mucosal IgG responses can also be increased. The p...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.9.975
更新日期:1992-09-01 00:00:00
abstract::Exosomes are small vesicles (60-100 nm) secreted by various cell types upon the fusion of endosomal compartments with the plasma membrane. Exosomes from antigen-presenting cells (APC), such as B lymphocytes and dendritic cells (DC), bear MHC class II molecules. In addition, the injection of DC-derived exosomes was rep...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf048
更新日期:2002-07-01 00:00:00
abstract::Neural stem cells (NSCs) transplantation has been proposed as a means of restoring damaged brain tissue, a possibility rendered more likely by reports of low NSCs immunogenicity in various experimental models because of low expression of MHC class I and II as well as co-stimulatory molecules. We investigated the immun...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm079
更新日期:2007-09-01 00:00:00
abstract::O-acetyl GD3 ganglioside is a cell surface molecule of some neural, neural crest and renal cells. Here we show, by using mAbs specific for O-acetyl GD3 (clone 27A) and flow-cytometric, biochemical or immunological techniques, that it is also expressed at high intensity level on the surface of 49.6% (median) of the CD3...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.9.1409
更新日期:1994-09-01 00:00:00
abstract::In order to better understand the role of intestinal CD1d, we sought to define the cellular localization and further characterize the biochemical structure of CD1d in human intestinal epithelial cells (IEC). Using a CD1d-specific rabbit anti-gst-CD1d antibody, immunoprecipitation of radiolabeled cell surface proteins ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.3.383
更新日期:1999-03-01 00:00:00
abstract::cDNA clones which strongly hybridized with a 3.1 kb mRNA from mouse macrophages and macrophage cell lines and weakly with mRNA from P815 but not from a variety of other cell lines and tissues were isolated from cDNA libraries constructed using mRNA from murine macrophage cell lines and peritoneal macrophages. Treatmen...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.6.585
更新日期:1990-01-01 00:00:00
abstract::Naturally arising CD25(+)CD4(+) regulatory T (T(R)) cells can be exploited to establish immunologic tolerance to non-self antigens. In vivo exposure of CD25(+)CD4(+) T cells from normal naive mice to alloantigen in a T cell-deficient environment elicited spontaneous expansion of alloantigen-specific CD25(+)CD4(+) T(R)...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh122
更新日期:2004-08-01 00:00:00
abstract::Fc receptors play important roles for a wide array of immune responses. In contrast to the well-defined Fcγ and Fcε receptors, the molecular and functional characteristics of Fc receptors for IgA and IgM have remained incompletely understood for years. Recent progress has unveiled the characteristics of Fc receptors f...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxx059
更新日期:2017-12-30 00:00:00
abstract::We have analyzed transcripts encoding the variable regions of Ig heavy chains from adult and fetal bovine splenocytes and bovine x mouse heterohybridomas. The 13 adult, seven fetal and two heterohybridomas transcripts as well as the six genes that were sequenced had > 83% identity to each other in the VH-encoded regio...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.1.189
更新日期:1997-01-01 00:00:00
abstract::One of the characteristic features of asthma is chronic airway inflammation typically with eosinophil infiltration. Most asthmatics can be treated successfully with conventional treatment appropriate for their severity, but in some severe cases, asthma cannot be well controlled even with thorough treatment and this co...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxx045
更新日期:2017-07-01 00:00:00
abstract::We investigated the role of IL-7 receptor alpha (IL-7Ralpha) signal in the formation of Peyer's patch (PP) anlage. Although pan-lymphopenia is a common phenotype of rag2-/- and il7ralpha-/- mice, a close inspection revealed nodules corresponding to PP in the adult rag2-/- but not in the il7ralpha-/- mouse. In our prev...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.1.1
更新日期:1998-01-01 00:00:00
abstract::Southern blot analysis of genomic DNA of normal mouse thymocytes with a JH4 probe, a probe for JH4 of Igh genes, consistently showed a second band in addition to a germline band. The same band was also observed in analysis with a 5' DQ probe, a 5'-flanking sequence of DQ52, thus suggesting the use of DQ52. By analyzin...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/1.6.643
更新日期:1989-01-01 00:00:00
abstract::Intravenous sensitization of C57BL/6 (B6) mice with class II H-2-disparate B6-C-H-2bm12(bm12) resting B cells induced anti-bm12 CD4+ T cell tolerance as shown by hyporesponsiveness in the anti-bm12 mixed lymphocyte reaction (MLR). The present study investigated the mechanism(s) of the failure of bm12 B cells to stimul...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.2.159
更新日期:1996-02-01 00:00:00