Abstract:
:Two major B cell subpopulations were identified in the IgD- compartment of tonsils and subsequently isolated. They displayed the following phenotypes: CD10+CD38+CD44- (CD38+ B cells) and CD10-CD38-CD44+ (CD38- B cells). Of the CD38- B cells, 70% also expressed CD24 and CD39, whereas CD77 was specifically distributed on 40% of CD38+ B cells, suggesting an additional level of heterogeneity in the cellular composition of these two B cell types. Whereas the majority of CD38+ B cells were in cycle, most CD38- B cells were quiescent. Conversely, Bcl-2 was expressed in CD38- B cells but was not detected in CD38+ B cells. Of the CD38- B cells, 30% bore the homing receptor Leu-8/Mel-14, whereas CD38+ B cells lacked this marker. Thus, CD38- B cells have both survival capacity and migratory competence. Both subsets expressed surface (s) Igs which were mainly of the IgG class, implying that most of these cells have already undergone isotype switching. CD38- B cells proliferated vigorously and produced large amounts of IgG in response to cytokines, following ligation of slgs or CD40. In contrast, CD38+ B cells were only stimulated for DNA synthesis by a combination of IL-4 and anti-CD40 antibodies, and failed to differentiate into Ig-secreting cells regardless of the stimulus applied. We propose that CD38- B cells represent an extra-follicular mature B cell population which has been positively selected and rescued from apoptosis, whereas the CD38+ B cell subset is composed of germinal centre B cells.
journal_name
Int Immunoljournal_title
International immunologyauthors
Lagresle C,Bella C,Defrance Tdoi
10.1093/intimm/5.10.1259subject
Has Abstractpub_date
1993-10-01 00:00:00pages
1259-68issue
10eissn
0953-8178issn
1460-2377journal_volume
5pub_type
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journal_title:International immunology
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更新日期:1996-12-01 00:00:00
abstract::Cross-linking of the IgM antigen receptor on an immature B cell lymphoma (BKS-2) induces growth arrest and apoptosis. This is accompanied by down-regulation of the immediate early genes, egr-1 and c-myc, and a reduction in NF-kappaB activity. Anti-IgM-induced growth arrest and apoptosis of this murine B cell lymphoma ...
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更新日期:2003-01-01 00:00:00
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journal_title:International immunology
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更新日期:1991-05-01 00:00:00
abstract::To develop a comprehensive catalogue of phenotypic and functional parameters of human CD4(+) T cell differentiation stages, we have performed microarray gene expression profiling on subpopulations of human thymocytes and circulating naive CD4(+) T cells, including CD3(-)CD4(+)CD8(-) intrathymic T progenitor cells, CD3...
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abstract::Mercury-induced autoimmunity is characterized by a T cell-dependent B cell activation (mainly of IgG1 and IgE isotypes), production of anti-nucleolar autoantibodies (ANolA) and the formation of renal IgG deposits. The autoimmunity is to a large extent controlled by genetic factors. We studied 15 different inbred mouse...
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更新日期:2000-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/intimm/dxn129
更新日期:2009-02-01 00:00:00
abstract::Blocking T cell co-stimulatory signals by anti-B7-1/B7-2 mAb is an attractive approach to treat autoimmune diseases. However, anti-B7-1/B7-2 mAb treatment is known to exacerbate autoimmune diseases through mechanisms not fully understood. Tumor necrosis factor alpha (TNF-alpha) and reactive oxygen species (ROS) also p...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm012
更新日期:2007-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2007-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章,评审
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更新日期:2018-08-30 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxq042
更新日期:2010-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg120
更新日期:2003-10-01 00:00:00
abstract::NKT cells are characterized by their expression of an NKT-cell-specific invariant antigen-receptor α chain encoded by Vα14Jα18 gene segments. These NKT cells bridge the innate and acquired immune systems to mediate effective and augmented responses; however, the limited number of NKT cells in vivo hampers their analys...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxu057
更新日期:2014-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.4.569
更新日期:1999-04-01 00:00:00
abstract::Under equilibrium conditions, the affinities of five anti-IgG2a mAb isolated from virus-infected mice were comparable to other high-affinity auto-antibodies. Similar to rheumatoid factors, these anti-IgG2a auto-antibodies bound to aggregated or complexed IgG2a with 50 to 1500-fold higher avidity than their monomeric c...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.7.1125
更新日期:1995-07-01 00:00:00
abstract::We have investigated the B cell response to Mycobacterium leprae in leprosy patients and healthy controls. A comparison of Western-blotted proteins separated by two-dimensional gel electrophoresis and probed with pooled sera from LL and BT patients revealed distinct antigen recognition patterns for the two classificat...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.9.803
更新日期:1990-01-01 00:00:00
abstract::We developed a novel approach to probe the molecular basis of TCR recognition of the MHC class I-peptide complex and to determine how constraints placed on peptide binding by the class I molecule influence T cell recognition. We synthesized peptide pairs derived from the N- and C-terminal regions of class I peptide li...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.1.81
更新日期:1997-01-01 00:00:00
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journal_title:International immunology
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更新日期:2016-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.11.1721
更新日期:1995-11-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.5.785
更新日期:1994-05-01 00:00:00
abstract::Using a complete series of overlapping peptides, we have identified the T cell epitopes of a malaria vaccine candidate, the circumsporozoite (CS) protein, that are recognized by sporozoite-exposed residents of a non-endemic country. This protein and subunits from it are being considered as malaria sporozoite vaccine c...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/2.10.945
更新日期:1990-01-01 00:00:00
abstract::The chaperone glucose-regulated protein 94 (GRP94) has long been used to augment peptide presentation to T cells. This chaperone binds antigenic peptides, binds to receptors on professional antigen-presenting cells (APCs), activates these cells and after internalization, transfers the peptides to MHC class I for activ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl049
更新日期:2006-07-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxm149
更新日期:2008-04-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.10.1229
更新日期:1993-10-01 00:00:00
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journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.7.1085
更新日期:2000-07-01 00:00:00
abstract::Non-obese diabetic mice spontaneously develop a type 1 diabetes. The entry of leukocytes in the islets of Langerhans was studied in untreated and in irradiated mice. FITC-labeled cells from spleen, lymph nodes or bone marrow of healthy or diabetic donors did home to the inflamed islets of unmanipulated recipients. B a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.12.1905
更新日期:1995-12-01 00:00:00
abstract::The profile of cytokines produced by CD4 T cells is profoundly influenced by the presence of IL-12. Here we demonstrate that during re-stimulation of antigen-specific immune responses in vitro, antigen-primed lymph node cells from DBA/2 mice produced 3- to 30-fold more IL-12 than did cells from BALB/c mice, which are ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.10.1511
更新日期:1996-10-01 00:00:00