Abstract:
:The chaperone glucose-regulated protein 94 (GRP94) has long been used to augment peptide presentation to T cells. This chaperone binds antigenic peptides, binds to receptors on professional antigen-presenting cells (APCs), activates these cells and after internalization, transfers the peptides to MHC class I for activation of T cells. Here we show that all these activities reside within amino acids 1-355 of GRP94. This small fragment is sufficient to bind peptides, to bind and be taken up by the receptors CD91 and scavenger receptor type A on either dendritic cells or macrophages. The minimal construct can augment peptide presentation in culture and induce antigen-specific CTL in naive mice only because it loads APCs with the relevant peptide. Thus, the sequence 1-355 is the immunologically sufficient module of GRP94 and we propose that this 'mini-chaperone' can be used in immunotherapy of tumors and vaccine development.
journal_name
Int Immunoljournal_title
International immunologyauthors
Biswas C,Sriram U,Ciric B,Ostrovsky O,Gallucci S,Argon Ydoi
10.1093/intimm/dxl049subject
Has Abstractpub_date
2006-07-01 00:00:00pages
1147-57issue
7eissn
0953-8178issn
1460-2377pii
dxl049journal_volume
18pub_type
杂志文章abstract::The previously defined binding motif of MHC class I H-2Kd-restricted antigenic peptides consists of a Y residue in position P2 and a hydrophobic residue with a large aliphatic side chain (L, I, or V) in position P9/P10 of optimal 9- or 10-mer peptides. We show now that the presence of a charged or a F residue in posit...
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journal_title:International immunology
pub_type: 杂志文章
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