Abstract:
:The lectin complement pathway is initiated by binding of mannose-binding lectin (MBL) and MBL-associated serine protease (MASP) to carbohydrates. In the human lectin pathway, MASP-1 and MASP-2 are involved in the proteolysis of C4, C2 and C3. Here we report that the human MBL-MASP complex contains a new 22 kDa protein [small MBL-associated protein (sMAP)] bound to MASP-1. Analysis of the nucleotide sequence of sMAP cDNA revealed that it is a truncated form of MASP-2, consisting of the first two domains (i.e. the first internal repeat and the epidermal growth factor-like domain) with four different C-terminal amino acids. sMAP mRNAs are expressed in liver by alternative polyadenylation of the MASP-2 gene, in which a sMAP-specific exon containing an in-frame stop codon and a polyadenylation signal is used. The involvement of sMAP in the MBL-MASP complex suggests that the activation mechanism of the lectin pathway is more complicated than that of the classical pathway.
journal_name
Int Immunoljournal_title
International immunologyauthors
Takahashi M,Endo Y,Fujita T,Matsushita Mdoi
10.1093/intimm/11.5.859subject
Has Abstractpub_date
1999-05-01 00:00:00pages
859-63issue
5eissn
0953-8178issn
1460-2377journal_volume
11pub_type
杂志文章abstract::In vaccine development, a major objective is to induce strong, specific T cell responses. This might be obtained by targeting antigen to cell surface molecules that efficiently channel the antigen into endocytic compartments for loading of MHC molecules. Antibodies have been used to deliver antigen; however, it is imp...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf110
更新日期:2002-12-01 00:00:00
abstract::Liver fibrosis is commonly observed in chronic liver disease. However, the immunological mechanisms underlying hepatic fibrosis due to chronic inflammation are not well defined, mainly because suitable experimental models have not been established. We have found that weekly i.v. administration of concanavalin A (Con A...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.9.1491
更新日期:1999-09-01 00:00:00
abstract::To understand the nature of long-term Th immune responses, we investigated in the present study the TCRBV gene repertoire of CD4(+) T cells specific for the recall antigen tetanus toxoid (TT) in recipients of an allogeneic bone marrow transplantation (allo-BMT) at several time points after transplantation and in their...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.4.507
更新日期:2001-04-01 00:00:00
abstract::Specific antibodies increase antigen uptake and presentation by antigen-presenting cells via the B cell receptor in B cells or FcgammaR in dendritic cells. To determine whether the interaction between antibody and antigen could influence the set of peptides presented by MHC II molecules, we analyzed the presentation o...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.11.1863
更新日期:1999-11-01 00:00:00
abstract::Sera of patients suffering from systemic lupus erythematosus (SLE) frequently contain oligoclonal IgG autoantibodies with high affinity for the ribosomal protein L7 (rpL7). The humoral autoimmune response to rpL7 apparently is driven by antigen and T cell dependent. In order to analyze the T cell response to rpL7 we c...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.2.125
更新日期:1999-02-01 00:00:00
abstract::Dendritic cells (DC) are professional antigen-presenting cells that play a central role in the control of immunity. Mature DC are characterized by high expression levels of MHC and co-stimulatory molecules, and by the secretion of IL-12, a key cytokine for the priming of cytotoxic T lymphocytes. Here, we have compared...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh077
更新日期:2004-05-01 00:00:00
abstract::Mice with a targeted deletion of the T-bet gene exhibit spontaneous airway hyperresponsiveness (AHR), airway inflammation, enhanced recovery of T(h)2 cytokines from bronchoalveolar lavage fluid, sub-epithelial collagen deposition and myofibroblast transformation. Here we analyze the mechanisms responsible for the chro...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh281
更新日期:2005-08-01 00:00:00
abstract::One of the characteristic features of asthma is chronic airway inflammation typically with eosinophil infiltration. Most asthmatics can be treated successfully with conventional treatment appropriate for their severity, but in some severe cases, asthma cannot be well controlled even with thorough treatment and this co...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxx045
更新日期:2017-07-01 00:00:00
abstract::The ability of Bcl-2 to inhibit apoptotic cell death is well established. Several homologues of the bcl-2 gene, such as bax, bcl-x or mcl-1, have recently been identified. Like Bcl-2, both Bcl-XL and Mcl-1 appear to function as repressors of apoptotic cell death, whereas Bax facilitates it, indicating possible interac...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.11.1817
更新日期:1995-11-01 00:00:00
abstract::P-selectin is a Ca(2+)-dependent lectin that participates in leukocyte adhesion to vascular endothelium and platelets. Myeloid cells and a subset of T lymphocytes express carbohydrate ligands at the cell surface. Previously, we suggested that heat stable antigen (HSA/mouse CD24), an extensively glycosylated cell surfa...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.10.1557
更新日期:1995-10-01 00:00:00
abstract::To delineate the role of the IgH 3' enhancer in the regulation of Ig heavy (IgH) chain gene expression, mice harbouring rearranged IgH transgenes, with (PSVmu3) and without (PSVmu1) this element, were produced. RNA and protein analysis from the different transgenic lines revealed a 5- to 7-fold increase in the express...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.7.1149
更新日期:1996-07-01 00:00:00
abstract::Recent data suggest that initiation of signal transduction via type 1 Fc epsilon receptor (Fc epsilon RI) and other immunoreceptors is spatially constrained to lipid rafts. In order to better understand the complexity and function of these structures, we prepared mAb against lipid rafts from the rat basophilic leukemi...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/14.2.213
更新日期:2002-02-01 00:00:00
abstract::Macrophages are heterogeneous and plastic, and play several diverse functions in immune responses. Emerging data provide evidence of multiple roles for metabolic pathways in the control of macrophage effector functions. The diverse functions of macrophages are categorized into two main subsets: classical activated mac...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxaa017
更新日期:2020-06-26 00:00:00
abstract::The human IL-3 gene, located on chromosome 5, contains several cis-acting DNA sequences, i.e. CLE (conserved lymphokine element) and a GC-rich region, similar to the GM-CSF gene. To investigate the role of these elements, the 5' flanking region of the IL-3 gene was attached to a bacterial chloramphenicol acetyltransfe...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.3.245
更新日期:1991-03-01 00:00:00
abstract::Novel biomarkers of rheumatoid arthritis (RA), in addition to antibodies against cyclic citrullinated peptides, are required. Metabolome analysis is a promising approach to identify metabolite biomarkers for clinical diagnosis. We adopted a comprehensive nontargeted metabolomics approach combining capillary electropho...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxaa059
更新日期:2020-08-31 00:00:00
abstract::IL-12 has been shown to play a central role in cell-mediated inflammatory reactions through direct activation of T cells and NK cells. IL-12 also strongly influences humoral immunity but these effects have been thought to be indirect and caused by intermediary cytokines. Using flow cytometry, we now show that IL-12 di...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.12.1955
更新日期:1996-12-01 00:00:00
abstract::Despite intense recent interest, the suppressive mechanisms of regulatory CD25+CD4 T cells remain poorly understood. One deficiency in the field is the lack of in vivo models where the effects of regulatory CD25+CD4 T cells on antigen-specific responder T cells can be measured quantitatively. We describe one such mode...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh213
更新日期:2005-04-01 00:00:00
abstract::Constitutive expression of p35 and p40 IL-12 mRNA was detected in splenic macrophages isolated from aged mice. Macrophages were also implicated as the cell type responsible for the dysregulated IL-6 and tumor necrosis factor (TNF)-alpha commonly observed to be constitutively produced by lymphoid cells from aged donors...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.5.745
更新日期:1997-05-01 00:00:00
abstract::Deletion of alpha i2 subunit of heterotrimeric G proteins induces a 2-4-fold increase in the proportions of CD4 and CD8 single-positive (SP) thymocytes as compared with wild-type littermates, but how G alpha i2 is involved in thymocyte development is unknown. To determine a role for G alpha i2 in a specific developmen...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh204
更新日期:2005-03-01 00:00:00
abstract::The anti-CD20 chimeric monoclonal antibody rituximab mediates cytotoxicity in malignant B cells via multiple mechanisms, including complement-dependent cytotoxicity (CDC), antibody-dependent cellular cytotoxicity (ADCC) and direct induction of apoptosis. To optimize treatment of non-Hodgkin's lymphoma, a fuller unders...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxs048
更新日期:2012-08-01 00:00:00
abstract::We have evaluated the capacity of a novel, nanoparticle-based tumor vaccine to eradicate established tumors in mice. C57BL/6 mice were intradermally (i.d.) inoculated with ovalbumin (OVA)-expressing EG-7 tumor cells. When the tumor size reached 7-8 mm, the tumor-bearing mice were i.d. injected near the tumor-draining ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh381
更新日期:2006-03-01 00:00:00
abstract::Interleukin 3 (IL-3) promotes the survival and proliferation of hematopoietic cells of various lineages in culture. Like most other hematopoietic colony stimulating factors, its mode of action is unknown. However, binding of the lymphokine induces protein tyrosine phosphorylation and enhanced glucose transport in some...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/3.8.827
更新日期:1991-08-01 00:00:00
abstract::An alloreactive CTL clone, 27.B2, produced in the mouse strain combination B6.C-H-2bm1 (bm1) against C57BL/6 (B6) and nominally directed against the Kb molecule has been shown to be peptide dependent. The clone recognizes intact B6, but not intact bm1 or bm3 target cells. However, peptides recognized by 27.B2 CTL can ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.3.321
更新日期:1992-03-01 00:00:00
abstract::Recent studies point to glycolipid-enriched membrane (GEM) microdomains as the critical sites for TCR-mediated signal transduction. However, whether the TCR complex is localized in the GEM domain is not well-defined. In the present study, we analyzed localization of the TCR-CD3 complex in the GEM domain by isolating t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.9.1395
更新日期:1999-09-01 00:00:00
abstract::In this study, we explored the potential of human naive CD4(+) T cells to acquire regulatory properties upon stimulation. We demonstrated that, in vitro, pre-activated naive CD4(+)CD25(-)CD45RA(+) T cells could become anergic and suppressive CD4(+)CD25(+) T cells upon lower intensity TCR stimulation. These CD4(+)CD25(...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp068
更新日期:2009-09-01 00:00:00
abstract::Several cytokines and chemokines including chemokine (C-C motif) ligand-2 (CCL2) are induced in HIV-1 infection. However, the impact of HIV-1 viremia on CCL2 regulation is largely unknown. We utilized a DNA oligonucleotide microarray covering 110 inflammatory genes. Five genes were induced by at least 2-fold in PBMCs ...
journal_title:International immunology
pub_type: 临床试验,杂志文章
doi:10.1093/intimm/dxl078
更新日期:2006-10-01 00:00:00
abstract::Two peptides based on the complementarity-determining regions (CDR) 1 and 3 (pCDR1 and pCDR3) of a murine monoclonal anti-DNA autoantibody that expresses the common idiotype 16/6Id were shown to down-regulate systemic lupus erythematosus (SLE)-associated T cell responses and to prevent the development of clinical symp...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg026
更新日期:2003-02-01 00:00:00
abstract::MHC class II molecules associate with peptides in the endocytic pathway. Different endosomal locations for peptide loading of class II molecules, varying from early endosomes (EE) to lysosomes, have been assigned on the basis of subcellular fractionation experiments. We have determined the intracellular location of HL...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.5.625
更新日期:1996-05-01 00:00:00
abstract::In this study, we show that IFNalpha increases the chemotaxis of human B cells to CCL20, CCL21 and CXCL12 in a dose- and time-dependent manner. The effect was maximal with 2000 IU ml(-1) IFNalpha. It peaked at 24 h and decreased thereafter. At 24 h, IFNalpha had increased B-cell chemotaxis to CCL20 by 20 +/- 6.2% (n =...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh227
更新日期:2005-04-01 00:00:00
abstract::The class II trans-activator (CIITA) is a bi- or multi-functional domain protein which plays a critical role in the expression of MHC class II genes. We report that removal of the N-terminal 151 amino acids, encompassing all of the acidic domain but leaving intact the proline/serine/threonine-rich domain, results in a...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.10.1545
更新日期:1997-10-01 00:00:00