Abstract:
:In this study, we explored the potential of human naive CD4(+) T cells to acquire regulatory properties upon stimulation. We demonstrated that, in vitro, pre-activated naive CD4(+)CD25(-)CD45RA(+) T cells could become anergic and suppressive CD4(+)CD25(+) T cells upon lower intensity TCR stimulation. These CD4(+)CD25(+) T cells generated in vitro potently suppress the proliferation of allogenic CD4(+)CD25(-) T cells independently of cytokines and in a contact-dependent manner. Our data indicate that expression of Foxp3 is not necessary to induce the suppressive T cell activity. We demonstrate that these CD4(+)CD25(+) T cells are unresponsive upon re-stimulation and that their suppressive activity is transient. However, we showed that the anergy and the suppressive function could be reversed by increasing the stimulus and their level of activation. We concluded from our data that these anergy and suppressive activities are related to a fine tuning of TCR activation threshold.
journal_name
Int Immunoljournal_title
International immunologyauthors
Noël G,Brinster C,Semana G,Bruniquel Ddoi
10.1093/intimm/dxp068subject
Has Abstractpub_date
2009-09-01 00:00:00pages
1025-36issue
9eissn
0953-8178issn
1460-2377pii
dxp068journal_volume
21pub_type
杂志文章abstract::Earlier studies have demonstrated an over-expression of 9-O-acetylated sialoglycoconjugates (9-OAcSGs) on lymphoblasts, concomitant with high titers of anti-9-OAcSGs in childhood acute lymphoblastic leukemia (ALL). The present study was aimed to evaluate whether this high induction of anti-9-OAcSGs at disease presenta...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxh198
更新日期:2005-02-01 00:00:00
abstract::We have followed CD4 and CD8 TCR V beta repertoires during the acute phase of Trypanosoma cruzi infection in a resistant mouse strain (C57BL/6). No major changes were found in the V beta TCR distributions analyzed (covering roughly 40% of the TCR repertoire) in peripheral CD4 T lymphocytes, confirming the polyclonal n...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.3.387
更新日期:1994-03-01 00:00:00
abstract::The role of B7 co-stimulatory signaling in in vivo tumor rejection remains incompletely characterized. In particular, the relative competence of B7-1 (CD80) and B7-2 (CD86) to provide effective co-stimulus is not well defined, and the identification of the T cell co-stimulatory receptor that mediates B7 co-stimulation...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.6.791
更新日期:1998-06-01 00:00:00
abstract::Mercury-induced autoimmunity is characterized by a T cell-dependent B cell activation (mainly of IgG1 and IgE isotypes), production of anti-nucleolar autoantibodies (ANolA) and the formation of renal IgG deposits. The autoimmunity is to a large extent controlled by genetic factors. We studied 15 different inbred mouse...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/12.10.1425
更新日期:2000-10-01 00:00:00
abstract::The class II transactivator (CIITA) controls both the constitutive and IFN-gamma inducible expression of HLA-D genes. In addition, through the squelching of another transactivator CREB-binding protein, CIITA was more recently shown to have a wider cellular function, including cell cycle control or cellular response to...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxf060
更新日期:2002-08-01 00:00:00
abstract::HLA-B*0702 transgenic mice (expressing a chimeric heavy chain with a murine alpha 3 domain: HLA-B7(m alpha 3)) in which the H-2K(b) and H-2D(b) class I-a (Cl I-a(-/-)) genes have been inactivated were compared with H-2K(b)D(b) Cl I-a(+/+) positive controls. Expression of the HLA-B7(m alpha 3) molecules resulted in a 3...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg073
更新日期:2003-06-01 00:00:00
abstract::Insulitis develops in non-obese diabetic (NOD) mice as a multicentric and asynchronous process. In an effort to understand how this T cell mediated process expands within each islet and propagates between the islets of the same pancreas, we have analyzed the junctional diversity of TCR V beta 6 and V beta 8.2 transcri...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.1.139
更新日期:1995-01-01 00:00:00
abstract::The utilization of individual VH7183 genes in the autoimmune non-obese diabetic (NOD) mouse and the normal C57BL/6 mouse, both carrying the IgHb haplotype, was investigated. Nucleotide sequence analyses of a large number of VHDJH rearrangements utilizing VH7183 gene segments allowed us to identify 10 individual member...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.4.623
更新日期:1994-04-01 00:00:00
abstract::Leishmania donovani resides within the host macrophages by dampening host defence mechanisms and thereby it modulates the host cell functions for its survival. Multiple host cell factors compete during the interplay between the host and the parasite. Roles for dual-specificity phosphatases (DUSPs) are implicated in va...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxw049
更新日期:2016-12-01 00:00:00
abstract::BALB.xid mice, carrying an X-linked mutation leading to the absence of CD5+ B cells, are highly resistant to Trypanosoma cruzi infection. These mice clear blood parasites in the acute phase of infection and do not develop the inflammatory infiltration characteristically observed in the chronic phase of susceptible str...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.8.1213
更新日期:1996-08-01 00:00:00
abstract::Taking advantage of the recently demonstrated presence of N-aminopeptidases and the serine protease dipeptidyl aminopeptidase IV (DPP IV) at the surface of human myeloblastic HL-60 cells, the regulation of these protease activities in HL-60 cell differentiation has been assessed using combined spectrophotometric and f...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.8.965
更新日期:1993-08-01 00:00:00
abstract::We established an experimental system in vitro to examine the developmental capacity of individual hematopoietic progenitors to generate T, B and myeloid (M) cells. By using this system we analyzed the process of lineage commitment of hematopoietic progenitors in murine fetal liver (FL). It is known that small numbers...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/9.7.1011
更新日期:1997-07-01 00:00:00
abstract::The transcription factor interferon regulatory factor-5 (IRF5) plays an important role in innate immune responses via the TLR-MyD88 (Toll-like receptor - myeloid differentiation primary response 88) pathway. IRF5 is also involved in the pathogenesis of the autoimmune disease systemic lupus erythematosus (SLE). Recent ...
journal_title:International immunology
pub_type: 杂志文章,评审
doi:10.1093/intimm/dxy032
更新日期:2018-10-29 00:00:00
abstract::Responsiveness of dendritic cells (DC) to inflammatory CC chemokines is down-regulated during their maturation. We analyzed the mechanism underlying these events. Cell-surface expression of CC chemokine receptor (CCR)-1, -3 and -5 was increased during differentiation of immature DC (iDC) from monocytes. In contrast, t...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/13.2.167
更新日期:2001-02-01 00:00:00
abstract::BALB/c mice injected at birth with 10(8) semi-allogeneic (C57BL/6 x BALB.IgHb)F1 spleen cells develop a lupus-like syndrome in which autoantibodies bear exclusively the donor allotype. We have analyzed the evolution of donor B cell chimerism and the autoimmune manifestations during the first year of life in these mice...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.1.67
更新日期:1992-01-01 00:00:00
abstract::We report here that different organ grafts are not equally competent to induce tolerance of the host even if they are performed early in development, i.e. before the host's immune system has started to develop. We have grafted the epithelio-mesenchymal rudiment of the bursa of Fabricius between histoincompatible chick...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/6.6.795
更新日期:1994-06-01 00:00:00
abstract::Analyses of bone marrow (BM) lymphocytes in C57BL/6 mice homozygous for the lpr mutation (B6.lpr) disclosed low numbers of pre-B and B cells, as compared with age-matched control B6 mice. BM depletion in B6.lpr mice was selective for B-lineage cells, appeared in young adults, and developed markedly with age and diseas...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.2.247
更新日期:1996-02-01 00:00:00
abstract::The immunomodulator FTY720 inhibits lymph node (LN) and thymic egress, thereby constraining T cell circulation and reducing peripheral T cell numbers. Here, we analyzed in mouse models the as yet scarcely characterized impact of long-term (up to 6 months) FTY720 exposure on T cell homeostasis and possible consequences...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxn023
更新日期:2008-05-01 00:00:00
abstract::Plasmid DNA vectors have been constructed with minigenes encoding a single cytotoxic T lymphocyte (CTL) epitope from either the M2 protein of respiratory syncytial virus (RSV) or from the nucleoprotein of measles virus (MV) with or without a signal sequence (also called secretory or leader sequence). Following intrade...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/10.10.1441
更新日期:1998-10-01 00:00:00
abstract::We have determined the origin and cell surface phenotype of B cells producing antibody in response to immunization with the non-self TI-2 antigen polyvinyl pyrrolidinone (PVP). We report that the responding cells are derived from precursors in adult bone marrow and display the phenotype characteristic of B-1 cells. By...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.4.533
更新日期:1996-04-01 00:00:00
abstract::Cyclosporin A (CsA) prevents most immature thymocytes from progressing to a mature phenotype and blocks the deletion of T cells that express self-specific TCR in the small population of cells that achieve maturity. The latter effect may explain the paradoxical observation that this immunosuppressive drug can induce au...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/4.12.1341
更新日期:1992-12-01 00:00:00
abstract::The TCR is responsible for the specificity of cytotoxic T lymphocytes (CTL) by recognizing peptides presented in the context of MHC. By producing recombinant soluble TCR, it is possible to study this interaction at the molecular level. We generated single-chain TCR (scTCR) from tumor infiltrating lymphocytes (TIL) and...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/11.5.745
更新日期:1999-05-01 00:00:00
abstract::Chemokine receptors (CCRs) are important co-stimulatory molecules found on many blood cells and associated with various diseases. The expression and function of CCRs on mast cells has been quite controversial. In this study, we report for the first time that murine bone marrow-derived mast cells (BMMC) express messeng...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxp066
更新日期:2009-08-01 00:00:00
abstract::NK cells limit the emergence of cancers and viral infections by surveillance of 'missing-self' and 'induced-self' ligands, and by direct recognition of pathogen-associated molecules. We examined individual roles for Toll-like receptors (TLRs)-7 and -8 in human NK-cell activation using synthetic, small molecule agonist...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxl046
更新日期:2006-07-01 00:00:00
abstract::In the final analysis, the self (S)-non-self (NS) discrimination is regulated by the sufficiency or insufficiency of effector T(h) (eT(h)) specific for the given antigen. We have described a model (Th genesis) for the origin of eT(h) based on an antigen-independent pathway from initial state T(h) (iT(h)) to eT(h), and...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxg059
更新日期:2003-05-01 00:00:00
abstract::The M54 transgenic mouse line, which carries the 17.2.25 Ig mu heavy chain gene, rearranges Ig heavy chains and expresses both transgenic and endogenous mu. B cell lineage development is selectively impaired in these mice and cells that simultaneously express transgenic and endogenous mu ('double-producers') are commo...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/5.9.1011
更新日期:1993-09-01 00:00:00
abstract::The profile of cytokines produced by CD4 T cells is profoundly influenced by the presence of IL-12. Here we demonstrate that during re-stimulation of antigen-specific immune responses in vitro, antigen-primed lymph node cells from DBA/2 mice produced 3- to 30-fold more IL-12 than did cells from BALB/c mice, which are ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/8.10.1511
更新日期:1996-10-01 00:00:00
abstract::Under equilibrium conditions, the affinities of five anti-IgG2a mAb isolated from virus-infected mice were comparable to other high-affinity auto-antibodies. Similar to rheumatoid factors, these anti-IgG2a auto-antibodies bound to aggregated or complexed IgG2a with 50 to 1500-fold higher avidity than their monomeric c...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/7.7.1125
更新日期:1995-07-01 00:00:00
abstract::In humans, the thymus is the primary lymphoid organ able to support the development of T cells through its three-dimensional (3D) organization of the thymic stromal cells. Since a remarkable number of similarities are shared between the thymic epithelial cells (TECs) and skin-derived keratinocytes and fibroblasts, in ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxt035
更新日期:2013-12-01 00:00:00
abstract::Natural killer (NK) cells are a major FcγRIIIA-expressing lymphocyte population that mediate antibody-dependent cellular cytotoxicity. Although NK cells are critical for immunity against viruses and tumors, they are also activated in the joints of patients with rheumatoid arthritis (RA) and may be involved in disease ...
journal_title:International immunology
pub_type: 杂志文章
doi:10.1093/intimm/dxz010
更新日期:2019-04-26 00:00:00